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Kalypsys Inc pintool kalypsys-gnf
Pintool Kalypsys Gnf, supplied by Kalypsys Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pintool/pintool+kalypsys/pm39824442-74-15-16
Average 90 stars, based on 1 article reviews
pintool kalypsys-gnf - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Control:

Article Title: Large-scale human myeloma cell line small molecule compound screen dataset.
Article Snippet: As previously described7, a Multidrop Combi dispenser (Thermo Fisher) was used to dispense a total of 10^3 cells/well in 5μl of media (described above) into 1536 well solid-bottom white Greiner Bio-one tissue culture plates. .. A Kalypsys pintool was used to dispense 23 nl of control compound (bortezomib) and library compounds to generate the dose response curves. .. The 1536 well plates were covered with Kalypsys stainless steel cell culture lids and incubated at 37°C with 5% CO2 and 95% humidity.

Incubation:

Article Title: Small-molecule screening identifies Syk kinase inhibition and rutaecarpine as modulators of macrophage training and SARS-CoV-2 infection.
Article Snippet: Cells were lifted using cell strip buffer and scraping, counted and plated into 1536 well plates (catalog # 789173-F) using a small cassette and a Multidrop Combi dispenser (Thermo Fisher Scientific Inc.,Waltham, MA) in 4 mL volume/well at a density of 6,000 cells per well. .. Following overnight incubation, compounds were added using a Kalypsys pintool (Columns 5–48) in serial dilution over a 7-point dose range. .. After 30 min, cells were stimulated with LPS (100 ng/mL) for 4 h at 37 C, 5%CO2, 95% humidity covered with low evaporation stainless steel lids from Kalypsys.

Serial Dilution:

Article Title: Small-molecule screening identifies Syk kinase inhibition and rutaecarpine as modulators of macrophage training and SARS-CoV-2 infection.
Article Snippet: Cells were lifted using cell strip buffer and scraping, counted and plated into 1536 well plates (catalog # 789173-F) using a small cassette and a Multidrop Combi dispenser (Thermo Fisher Scientific Inc.,Waltham, MA) in 4 mL volume/well at a density of 6,000 cells per well. .. Following overnight incubation, compounds were added using a Kalypsys pintool (Columns 5–48) in serial dilution over a 7-point dose range. .. After 30 min, cells were stimulated with LPS (100 ng/mL) for 4 h at 37 C, 5%CO2, 95% humidity covered with low evaporation stainless steel lids from Kalypsys.

other:

Article Title: Method of identifying a compound which affects the multienzyme metabolic assembly of glucose metabolism and its association with cell cycle progression in cancer cells
Article Snippet: Again, 23 nL of compounds were transferred by a pintool (Kalypsys) in the final concentration range of 974 pM to 57.5 μM along with DMSO.

Article Title: A Comprehensive Phenotypic Screening Strategy to Identify Modulators of Cargo Translocation by the Bacterial Type IVB Secretion System
Article Snippet: The compounds were transferred into 1,536-well plates using a Kalypsys pintool or manually into 96-well plates.

Article Title: A high throughput assay for phosphoribosylformylglycinamidine synthase.
Article Snippet: After addition of 30 nL of vehicle (75 % v/v DMSO) or compounds using a pintool (Kalypsys-GNF), 2.5 μL of assay buffer and PFAS were dispended into column 1–3 and 4–48, respectively.

Article Title: Cytotoxic Profiling of Annotated and Diverse Chemical Libraries Using Quantitative High-Throughput Screening.
Article Snippet: A pin tool (Kalypsys, San Diego, CA) was used to transfer 23 nL of compound solution to the 1536-well assay plates.

Concentration Assay:

Article Title: Physicochemical Characterization of a Thermostable Alcohol Dehydrogenase from Pyrobaculum aerophilum
Article Snippet: .. Using a Kalypsys pin tool equipped with 1,536-pins, 23 nL of compound solution was transferred to the assay plate, resulting in a final DMSO concentration <0.5%. ..

Magnetic Beads:

Article Title: Identification of Small Molecule Inhibitors of a Mir155 Transcriptional Reporter in Th17 Cells
Article Snippet: For the HTS assay, 2000 differentiated Th17 cells/well in Th17 polarizing media were added to Greiner-one high base solid bottom white tissue culture treated 1536 well plates (catalog # 789173-F) using a small cassette and a Multidrop Combi dispenser (Thermo Fisher Scientific Inc.,Waltham, MA). .. Compounds were added using a Kalypsys pintool (Columns 5–48) and magnetic beads coupled to anti-CD3 and anti-CD28 (CD3/CD28 microbeads) were added to mediate T cell activation. ..

Activation Assay:

Article Title: Identification of Small Molecule Inhibitors of a Mir155 Transcriptional Reporter in Th17 Cells
Article Snippet: For the HTS assay, 2000 differentiated Th17 cells/well in Th17 polarizing media were added to Greiner-one high base solid bottom white tissue culture treated 1536 well plates (catalog # 789173-F) using a small cassette and a Multidrop Combi dispenser (Thermo Fisher Scientific Inc.,Waltham, MA). .. Compounds were added using a Kalypsys pintool (Columns 5–48) and magnetic beads coupled to anti-CD3 and anti-CD28 (CD3/CD28 microbeads) were added to mediate T cell activation. ..



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