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Proteintech phosphorylated c src
Phosphorylated C Src, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 2375 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phosphorylated+src/CEL+Antibody/pm41399133-75-5-12
Average 96 stars, based on 2375 article reviews

phosphorylated c src - by Bioz Stars, 2026-10

96/100 stars

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Incubation:

Article Title: Monochromatic light affects intestinal barrier function of chick via an anti-oxidation pathway involving melatonin and its receptor Mel1b
Article Snippet: .. Paraffin jejunal sections were incubated with primary antibodies at 4°C overnight: proliferating cell nuclear antigen (PCNA, 1:300; Proteintech, Wuhan, China), IgA (1:300; Abcam, Cambridge, England), Mel 1a (1:200; Abmart, Shanghai, China), Mel 1b (1:300; Abcam, USA) and Mel 1c (1:100; Abmart, Shanghai, China). .. Then, the sections were rinsed with PBS (0.01 M, pH = 7.4) and incubated with biotinylated secondary antibodies (Goat-anti-Mouse/Goat-anti-Rabbit, 1:300, CWBio, Taizhou, China) and streptavidin-horseradish peroxidase (1:300, Vector Laboratories, Burlingame, CA, USA) successively for 2 h at room temperature.

Article Title: A paracrine-to-autocrine shunt of GREM1 fuels colorectal cancer metastasis via ACVR1C.
Article Snippet: .. Subsequently, the samples were incubated with goat anti-GREM1 (3 μg/mL, R&D, AF956), mouse anti-β-Catenin (1:100, BD, 610154), rabbit anti-β-Catenin (1:100, Absea, RC-6352), rabbit anti-Vimentin (1:100, CST, 5741), rabbit anti-CD68 (1:100, CST, 26042), rabbit anti-FAP (1:50, Proteintech, 15384-1-AP), rabbit anti-α-SMA (1:100, Abcam, ab5694), rabbit anti-E-Cadherin (1:200, CST, 3195), rabbit antiACVR1C (1:50, Thermo, PA587475), rabbit anti-Snail (1:200, Abcam, ab224731) and mouse anti-CDX2 (1:200, Servicebio, GB151501) overnight at 4 °C. .. The tissues were incubated with Alexa-Fluor-conjugated secondary antibodies (Invitrogen) in AR TIC LE IN PR ES S PBS with 1 % normal donkey serum for 1 h at room temperature.

Article Title: Islet regeneration protein Reg3g promotes macrophage clearance of β cell-derived dysfunctional mitochondria-rich vesicles to mitigate T2DM
Article Snippet: .. And then cell slides were incubated with primary antibodies, including LC3A/B (#12741, CST), and P2RX7(28207-1-AP, Proteintech) at 4 °C overnight. ..

Article Title: Remote Pancreatic Response to Severe Polytrauma Correlates With Clinical Outcome
Article Snippet: After UV activation using a Chemidoc XRS+ (Biorad), proteins were blotted onto a PVDF membrane and total lane protein was measured using the Chemidoc imager. .. Membranes were blocked with 5% BSA in 0.1% Tween20 (Sigma, Taufkirchen, Germany) in TBS (TBS‐T) for 1 h at 4°C, and incubated with anti‐Cel (Proteintech, 15384‐1‐AP, 1:2000) and anti‐Pnlip (Proteintech, 11209‐1‐AP, 1:2000) antibodies o/n at 4°C. .. After washing three times with TBS‐T, 1:5000 ECL anti‐rabbit horseradish peroxidase (HRP) IgG secondary antibody (GE Healthcare, Duesseldorf, Germany) was added for 1 h at RT.

Cell Culture:

Article Title: The sphingolipid metabolite sphingosine protects against hypertension by targeting metabolic-inflammatory crosstalk via the NLRP3 inflammasome
Article Snippet: .. The concentrations of secreted proinflammatory cytokines in the cell culture supernatant were quantified using commercial ELISA kits for human IL-1β (Proteintech, Cat# KE00021) and human IL-18 (Proteintech, Cat# KE00193), following the manufacturer's instructions. ..

Enzyme-linked Immunosorbent Assay:

Purification:

Article Title: E2F1 K117 methylation by SETD6 disrupts BRD4–E2F1 binding and modulates E2F1 chromatin binding and gene regulation in prostate cancer cells
Article Snippet: Next, cells were washed with 1× Phosphate Buffered Saline (PBS) buffer (Gibco) and harvested by centrifugation at 500 g for 5 min. After harvesting, the amount of proteins in whole cell lysate was determined by western-blot. .. For protein/protein interaction analysis, the GFP-fused proteins were purified from the cell extract using GFP-trap A beads (Chromotek) according to the manufacturer’s instructions. ..

Transcranial Magnetic Stimulation:

Blocking Assay:

Article Title: TLR2-PI3K/Akt mediated microbe-mimetic priming boosts the therapeutic paracrine function of GelMA-Encapsulated MSCs for diabetic wound regeneration
Article Snippet: After being centrifuged, quantified and denatured, 80 μg protein samples were separated by 12 % sodium dodecyl sulfate polyacrylamide gel electrophoresis and were then transferred onto methanol-activated polyvinylidene difluoride membranes (GE Healthcare, USA). .. Bovine serum albumin solutions (5 %) were used to block non-specific antigens for 1.5 h. The membranes were probed with anti-PI3K rabbit monoclonal antibody (1:500, C67E7, Cell siginting), anti-Phospho-Akt (Ser473) rabbit monoclonal antibody (1:500, 9271T, Cell siginting), anti- P13K rabbit monoclonal antibody (1:500, C73F8, Cell siginting), anti-p-PI3K rabbit monoclonal antibody (1:500, ab278545, Abcam) or anti-GAPDH mouse monoclonal antibody (1:1000, 60 004–1-Ig, Proteintech). .. Protein bands were visualized using a UVITEC Alliance MINI HD9 instrument (UVITEC, UK) with an enhanced chemiluminescence detection system.

Image Search Results

DAPA and alirocumab inhibited lipid droplet formation and cell stress signaling depended on suppressing the expression of receptor CD36. (A) Protein expression of Fyn, * vs. other groups with different symbols (†, ‡), P < 0.0001. (B) Protein expression of Lyn, * vs. other groups with different symbols (†, ‡), P < 0.0001. (C) Protein expression of phosphorylated (p)-Src, * vs. other groups with different symbols (†, ‡), P < 0.0001. (D) Protein expression of p-MKKK, * vs. other groups with different symbols (†, ‡), P < 0.0001. (E) Protein expression of p-JNK, * vs. other groups with different symbols (†, ‡), P < 0.0001. (F) Protein expression of p-c-JUN, * vs. other groups with different symbols (†, ‡), P < 0.0001. (G) Protein expression of p-p38, * vs. other groups with different symbols (†, ‡), P < 0.0001. (H) Protein expression of p-ERK1/2, * vs. other groups with different symbols (†, ‡), P < 0.0001. (I) Protein expression of NF-κB, * vs. other groups with different symbols (†, ‡), P < 0.0001. (J) Protein expression of PPAR-γ, * vs. other groups with different symbols (†, ‡), P < 0.0001. (K) Protein expression of NOX2, * vs. other groups with different symbols (†, ‡), P < 0.0001. (L) Analytical result of ANGPTL4 activity by ELISA, * vs. other groups with different symbols (†, ‡, §, ¶, α, γ), P < 0.001. (M) Analytical result of lipoprotein lipase activity (LPLa) by ELISA, * vs. other groups with different symbols (†, ‡, §, ¶, α, γ), P < 0.0001. All statistical analyses were performed by one-way ANOVA, followed by Bonferroni multiple comparison post hoc test ( n =3 for WB in each group). Symbols (*, †, ‡, §, ¶, α, γ) indicate significance (at 0.05 level). (N–T) Illustrating the lipid droplet formation in a macrophage (gray color) in different conditions. (U) Analytical result of lipid droplet formation * vs. other groups with different symbols (†, ‡), P < 0.0001 ( n = 5). Cell grouping : F1 = RAW 264.7 cells, F2 = RAW 264.7 cells + FFA (TG), F3 = RAW 264.7 cells + FFA (TG) + DAPA (50 μM), F4 = RAW 264.7 cells + FFA (TG) + Alirocumab (200 nM), F5 = siRNA CD36 in RAW 264.7 cells + FFA, F6 = siRNA CD36 in RAW 264.7 cells + FFA + DAPA (50 μM), F7 = siRNACD36 in RAW 264.7 cells + FFA + Alirocumab (200 nM). FFA, free fatty acid.

Journal: International Journal of Surgery (London, England)

Article Title: CD36 serves as a signaling receptor and free fatty acid (FFA) transporter in dyslipidemia: the role of dapagliflozin and alirocumab in downregulating atherogenic dyslipidemia via FFA uptake inhibition—experimental study

doi: 10.1097/JS9.0000000000003451

Figure Lengend Snippet: DAPA and alirocumab inhibited lipid droplet formation and cell stress signaling depended on suppressing the expression of receptor CD36. (A) Protein expression of Fyn, * vs. other groups with different symbols (†, ‡), P < 0.0001. (B) Protein expression of Lyn, * vs. other groups with different symbols (†, ‡), P < 0.0001. (C) Protein expression of phosphorylated (p)-Src, * vs. other groups with different symbols (†, ‡), P < 0.0001. (D) Protein expression of p-MKKK, * vs. other groups with different symbols (†, ‡), P < 0.0001. (E) Protein expression of p-JNK, * vs. other groups with different symbols (†, ‡), P < 0.0001. (F) Protein expression of p-c-JUN, * vs. other groups with different symbols (†, ‡), P < 0.0001. (G) Protein expression of p-p38, * vs. other groups with different symbols (†, ‡), P < 0.0001. (H) Protein expression of p-ERK1/2, * vs. other groups with different symbols (†, ‡), P < 0.0001. (I) Protein expression of NF-κB, * vs. other groups with different symbols (†, ‡), P < 0.0001. (J) Protein expression of PPAR-γ, * vs. other groups with different symbols (†, ‡), P < 0.0001. (K) Protein expression of NOX2, * vs. other groups with different symbols (†, ‡), P < 0.0001. (L) Analytical result of ANGPTL4 activity by ELISA, * vs. other groups with different symbols (†, ‡, §, ¶, α, γ), P < 0.001. (M) Analytical result of lipoprotein lipase activity (LPLa) by ELISA, * vs. other groups with different symbols (†, ‡, §, ¶, α, γ), P < 0.0001. All statistical analyses were performed by one-way ANOVA, followed by Bonferroni multiple comparison post hoc test ( n =3 for WB in each group). Symbols (*, †, ‡, §, ¶, α, γ) indicate significance (at 0.05 level). (N–T) Illustrating the lipid droplet formation in a macrophage (gray color) in different conditions. (U) Analytical result of lipid droplet formation * vs. other groups with different symbols (†, ‡), P < 0.0001 ( n = 5). Cell grouping : F1 = RAW 264.7 cells, F2 = RAW 264.7 cells + FFA (TG), F3 = RAW 264.7 cells + FFA (TG) + DAPA (50 μM), F4 = RAW 264.7 cells + FFA (TG) + Alirocumab (200 nM), F5 = siRNA CD36 in RAW 264.7 cells + FFA, F6 = siRNA CD36 in RAW 264.7 cells + FFA + DAPA (50 μM), F7 = siRNACD36 in RAW 264.7 cells + FFA + Alirocumab (200 nM). FFA, free fatty acid.

Article Snippet: The membranes were incubated with the indicated primary antibodies [CD36 (1:4000, ABCAM), PPARr (1:1000, Cell signaling), Fyn (1:1000, Cell signaling), Lyn (1:1000, Cell signaling), phosphorylated (p)-Src (1:1000, Cell signaling), p-ERK1/2 (1:3000, Merck), NOX2 (1:4000, Sigma), p-JNK (1:3000, Abcam), p-c-Jun (1:3000, Abcam), IL-1β (1:1000, Cell signaling), p-nuclear factor keppa B (p-NF-κB) (1:1000, Cell signaling), p-MMK4 (1:4000, Abcam), p-p38 (1:4000, Sigma), ANGPTL4 (1:4000, Abcam), NOD-like receptor protein 3 (NLRP3) (1:4000, Abcam), cleaved caspase1 (1:4000, novusbio), interleukin (IL)-18 (1:3000, Abcam), and Actin (1:12 000, Merck)] for 1 hour at room temperature.

Techniques: Expressing, Activity Assay, Enzyme-linked Immunosorbent Assay, Comparison

FIGURE 2 Dose-dependent effects of chronic administration of clozapine on expression of pannexin1 and phosphorylated Src in the mPFC. Rats were chronically administered with clozapine (0, 5 and 10 mg kg1 day1 for 28 days). In the upper histograms, ordinate: mean ± SD (n = 6) of the relative levels of pannexin1 (PANX) per GAPDH (a) and phosphorylated-Src (pSrc) per Src (b). *P < 0.05: relative to control (clozapine free) by one-way ANOVA with Scheffe's post hoc test. F-values of the chronic clozapine administration on the expression of pannexin1 and pSrc in the rat frontal cortex using one-way ANOVA were [F(3,15) = 17.3 (P < 0.01)] and [F(3,15) = 18.0 (P < 0.01)], respectively. The lower panels indicate pseudo-gel images of capillary immunoblotting.

Journal: British journal of pharmacology

Article Title: Exploring the pathophysiology underlying clozapine-induced enhancement of glutamatergic transmission through L-glutamate and D-serine release associated with pannexin1 hemichannels.

doi: 10.1111/bph.70112

Figure Lengend Snippet: FIGURE 2 Dose-dependent effects of chronic administration of clozapine on expression of pannexin1 and phosphorylated Src in the mPFC. Rats were chronically administered with clozapine (0, 5 and 10 mg kg1 day1 for 28 days). In the upper histograms, ordinate: mean ± SD (n = 6) of the relative levels of pannexin1 (PANX) per GAPDH (a) and phosphorylated-Src (pSrc) per Src (b). *P < 0.05: relative to control (clozapine free) by one-way ANOVA with Scheffe's post hoc test. F-values of the chronic clozapine administration on the expression of pannexin1 and pSrc in the rat frontal cortex using one-way ANOVA were [F(3,15) = 17.3 (P < 0.01)] and [F(3,15) = 18.0 (P < 0.01)], respectively. The lower panels indicate pseudo-gel images of capillary immunoblotting.

Article Snippet: Primary antibodies against GAPDH (NB300-327, RRID:AB_10001915, 1:300; Novus Biologicals, Littleton, CO, USA), pannexin1 (12595-1-AP, RRID:AB_ 2159186, 1:100; Proteintech, Rosemont, IL, USA), Src (2109, RRID: AB_2106059,1:50; Cell Signalling Technology, Danvers, MA, USA) and phosphorylated Src (pSrc; MAB2685, RRID:AB_3657975, 1 μg ml 1, R&D Systems, Minneapolis, MN, USA) were diluted in an antibody diluent (Immuno Shot Platinum; CosmoBio, Tokyo, Japan).

Techniques: Expressing, Control, Western Blot

$FIGURE 6 Concentration-dependent effects of chronic administration of clozapine on expression of pannexin1 and phosphorylated Src in cultured astrocytes. (a and b) Concentration-dependent effects of chronic exposure to clozapine (3 and 10 μM for 28 days) on expression of pannexin1 and phosphorylated Src in the astroglial plasma membrane fraction, respectively. In the upper histograms, ordinate: mean ± SD (n = 6) of the relative levels of pannexin1 (PANX) per GAPDH or phosphorylated Src (pSrc) per Src. *P < 0.05: relative to control (clozapine free) by one- way ANOVA with Scheffe's post hoc test. F-values of the concentration-dependent effects of chronic clozapine administration on the expression of pannexin1 and pSrc using one-way ANOVA were [F(2,15) = 21.3 (P < 0.01)] and [F(2,15) = 14.9 (P < 0.01)], respectively. The lower panels indicate pseudo-gel images of capillary immunoblotting.$

Journal: British journal of pharmacology

doi: 10.1111/bph.70112

Figure Lengend Snippet: FIGURE 6 Concentration-dependent effects of chronic administration of clozapine on expression of pannexin1 and phosphorylated Src in cultured astrocytes. (a and b) Concentration-dependent effects of chronic exposure to clozapine (3 and 10 μM for 28 days) on expression of pannexin1 and phosphorylated Src in the astroglial plasma membrane fraction, respectively. In the upper histograms, ordinate: mean ± SD (n = 6) of the relative levels of pannexin1 (PANX) per GAPDH or phosphorylated Src (pSrc) per Src. *P < 0.05: relative to control (clozapine free) by one- way ANOVA with Scheffe's post hoc test. F-values of the concentration-dependent effects of chronic clozapine administration on the expression of pannexin1 and pSrc using one-way ANOVA were [F(2,15) = 21.3 (P < 0.01)] and [F(2,15) = 14.9 (P < 0.01)], respectively. The lower panels indicate pseudo-gel images of capillary immunoblotting.

Techniques: Concentration Assay, Expressing, Cell Culture, Clinical Proteomics, Membrane, Control, Western Blot

FIGURE 7 Interaction among clozapine and inhibitors of Src and III-mGluR on the expression of pannexin1 and phosphorylated Src in the cultured astrocytes. (a) Interaction between 10 μM PP2 (Src inhibitor) and 10 μM clozapine on pannexin1 expression in the astroglial plasma membrane. (b) Interaction between 100 μM CPPG (III-mGluR inhibitor) and 10 μM clozapine on phosphorylated-Src expression in the astroglial plasma membrane. In the upper histograms, ordinate: mean ± SD (n = 6) of the relative levels of pannexin1 (PANX) per GAPDH or phosphorylated Src (pSrc) per Src. *P < 0.05: relative to control (clozapine free) by two-way ANOVA with Scheffe's post hoc test. @: P < 0.05: relative to 10 μM clozapine exposure by two-way ANOVA with Scheffe's post hoc test. F-values of the interaction between 10 μM PP2 and chronic exposure to 10 μM clozapine on the expression of pannexin1 using two-way ANOVA were [Fclozapine(1,20) = 55.5 (P < 0.01), FPP2(1,20) = 0.2 (P > 0.05), Fclozapine*PP2(1,20) = 1.1 (P > 0.05)]. F- values of the interaction between 100 μM CPPG and chronic exposure to 10 μM clozapine on the expression of pSrc using two-way ANOVA were [Fclozapine(1,20) = 56.0 (P < 0.01), FCPPG(1,20) = 27.7 (P < 0.01), Fclozapine*CPPG(1,20) = 0.4 (P > 0.05)]. The lower panels indicate pseudo-gel images of capillary immunoblotting.

Journal: British journal of pharmacology

doi: 10.1111/bph.70112

Figure Lengend Snippet: FIGURE 7 Interaction among clozapine and inhibitors of Src and III-mGluR on the expression of pannexin1 and phosphorylated Src in the cultured astrocytes. (a) Interaction between 10 μM PP2 (Src inhibitor) and 10 μM clozapine on pannexin1 expression in the astroglial plasma membrane. (b) Interaction between 100 μM CPPG (III-mGluR inhibitor) and 10 μM clozapine on phosphorylated-Src expression in the astroglial plasma membrane. In the upper histograms, ordinate: mean ± SD (n = 6) of the relative levels of pannexin1 (PANX) per GAPDH or phosphorylated Src (pSrc) per Src. *P < 0.05: relative to control (clozapine free) by two-way ANOVA with Scheffe's post hoc test. @: P < 0.05: relative to 10 μM clozapine exposure by two-way ANOVA with Scheffe's post hoc test. F-values of the interaction between 10 μM PP2 and chronic exposure to 10 μM clozapine on the expression of pannexin1 using two-way ANOVA were [Fclozapine(1,20) = 55.5 (P < 0.01), FPP2(1,20) = 0.2 (P > 0.05), Fclozapine*PP2(1,20) = 1.1 (P > 0.05)]. F- values of the interaction between 100 μM CPPG and chronic exposure to 10 μM clozapine on the expression of pSrc using two-way ANOVA were [Fclozapine(1,20) = 56.0 (P < 0.01), FCPPG(1,20) = 27.7 (P < 0.01), Fclozapine*CPPG(1,20) = 0.4 (P > 0.05)]. The lower panels indicate pseudo-gel images of capillary immunoblotting.

Techniques: Expressing, Cell Culture, Clinical Proteomics, Membrane, Control, Western Blot

FIGURE 9 Effects of chronic administration of L-BAIBA on Src signalling in the cultured astrocytes. (a and b) Interaction among chronic exposure to 30 μM L-BAIBA for 28 days and inhibitors of III-mGluR (100 μM CPPG) and NMDAR (1 μM MK801), respectively. In the upper side histogram, ordinate: mean ± SD (n = 6) of the relative levels of phosphorylated-Src (pSrc) per Src. *P < 0.05: relative to control (L-BAIBA free) by two-way ANOVA with Scheffe's post hoc test. @: P < 0.05: relative to 30 μM L-BAIBA exposure alone. F-values of the interaction between 100 μM CPPG and chronic exposure to 30 μM L-BAIBA on the expression of pSrc using two-way ANOVA were [FL-BAIBA(1,20) = 27.6 (P < 0.01), FCPPG(1,20) = 59.3 (P < 0.01), FL-BAIBA*CPPG(1,20) = 2.7 (P > 0.05)]. F-values of the interaction between 1 μM MK801 and chronic exposure to 30 μM L-BAIBA on the expression of pSrc using two-way ANOVA were [FL-BAIBA(1,20) = 46.3 (P < 0.01), FMK801(1,20) = 1.0 (P > 0.05), FL-

Journal: British journal of pharmacology

doi: 10.1111/bph.70112

Figure Lengend Snippet: FIGURE 9 Effects of chronic administration of L-BAIBA on Src signalling in the cultured astrocytes. (a and b) Interaction among chronic exposure to 30 μM L-BAIBA for 28 days and inhibitors of III-mGluR (100 μM CPPG) and NMDAR (1 μM MK801), respectively. In the upper side histogram, ordinate: mean ± SD (n = 6) of the relative levels of phosphorylated-Src (pSrc) per Src. *P < 0.05: relative to control (L-BAIBA free) by two-way ANOVA with Scheffe's post hoc test. @: P < 0.05: relative to 30 μM L-BAIBA exposure alone. F-values of the interaction between 100 μM CPPG and chronic exposure to 30 μM L-BAIBA on the expression of pSrc using two-way ANOVA were [FL-BAIBA(1,20) = 27.6 (P < 0.01), FCPPG(1,20) = 59.3 (P < 0.01), FL-BAIBA*CPPG(1,20) = 2.7 (P > 0.05)]. F-values of the interaction between 1 μM MK801 and chronic exposure to 30 μM L-BAIBA on the expression of pSrc using two-way ANOVA were [FL-BAIBA(1,20) = 46.3 (P < 0.01), FMK801(1,20) = 1.0 (P > 0.05), FL-

Techniques: Cell Culture, Control, Expressing

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phosphorylated c src (Proteintech)

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