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fosfomycin  (Thermo Fisher)


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    Structured Review

    Thermo Fisher fosfomycin
    Fosfomycin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphonomycin/Fosfomycin+disodium%2C+90%25/pmc13086064-87-49-56
    Average 94 stars, based on 1 article reviews
    fosfomycin - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Diffusion-based Assay:

    Article Title: IncL plasmid-mediated dissemination of OXA-48 β-lactamase and bla CTX-M-15 gene amplification identified via long-read sequencing in carbapenem-resistant Enterobacterales
    Article Snippet: Clinical and Laboratory Standards Institute (CLSI) breakpoints were applied for cefoperazone, netilmicin and tetracycline., Additionally, in carbapenemase-producing Enterobacterales (CPE) isolates, susceptibility to novel β-lactam-β-lactamase inhibitor combinations (βL-βLIs), including meropenem–vaborbactam, imipenem–relebactam, ceftazidime–avibactam and ceftolozane–tazobactam, was determined using the MIC test strip (bioMérieux, France). .. Susceptibility to cefiderocol was tested by the disc diffusion (Oxoid, UK) and to fosfomycin using the MIC test strip (Liofilchem, Italy). ..

    Stripping Membranes:

    Article Title: IncL plasmid-mediated dissemination of OXA-48 β-lactamase and bla CTX-M-15 gene amplification identified via long-read sequencing in carbapenem-resistant Enterobacterales
    Article Snippet: Clinical and Laboratory Standards Institute (CLSI) breakpoints were applied for cefoperazone, netilmicin and tetracycline., Additionally, in carbapenemase-producing Enterobacterales (CPE) isolates, susceptibility to novel β-lactam-β-lactamase inhibitor combinations (βL-βLIs), including meropenem–vaborbactam, imipenem–relebactam, ceftazidime–avibactam and ceftolozane–tazobactam, was determined using the MIC test strip (bioMérieux, France). .. Susceptibility to cefiderocol was tested by the disc diffusion (Oxoid, UK) and to fosfomycin using the MIC test strip (Liofilchem, Italy). ..

    AST Assay:

    Article Title: High prevalence of Panton-Valentine Leucocidin (PVL) toxin carrying MRSA and multidrug resistant gram negative bacteria in late onset neonatal sepsis indicate nosocomial spread in a Pakistani tertiary care hospital.
    Article Snippet: .. Commercially available antibiotic discs of azithromycin (ATM 15 μg), ceftazidime (30 μg), ceftriaxone (CRO 30 μg), nitrofurantoin (NIF 50 μg), cefaclor (CFC 30 μg), amoxicillin-clavulanic acid (AMC 20 +10 μg), gentamicin (CN 10 or 120 μg), cefuroxime (CXM 30 μg), ciprofloxacin (CIP 5 μg), penicillin (AST 6 μg), Piperacillin- tazobactam (TZP 75 +10 μg), Imipenem (IPM 10 μg) / meropenem (MEM 10 μg), levofloxacin (LVX 5 μg), amoxicillin (AMX 25 μg), tetracycline (TE 30 μg), tigecycline (TGC 15 μg), erythromycin (E 15 μg), vancomycin (V 5 μg), fosfomycin (FOS 50 μg), ampicillin (AMP 10 μg), doxycycline (DO 30 μg), amikacin (AK 30 μg) and clindamycin (DA 2 μg) purchased from Oxoid, UK were used. .. The discs were applied to Mueller Hinton (MH) agar, (Oxoid, UK) plates inoculate with pure isolates in form of lawn culture.



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    Effects of IFA (400 μg/mL) combined with antibiotics on bacterial viability of E. coli O157:H7. (A) <t>Fosfomycin</t> sodium. (B) Ceftriaxone. (C) Gentamicin. (D) Polymyxin B. (E) Streptomycin. (F) Chloramphenicol. (G) Tetracycline. (H) Azithromycin. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
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    Effects of IFA (400 μg/mL) combined with antibiotics on bacterial viability of E. coli O157:H7. (A) <t>Fosfomycin</t> sodium. (B) Ceftriaxone. (C) Gentamicin. (D) Polymyxin B. (E) Streptomycin. (F) Chloramphenicol. (G) Tetracycline. (H) Azithromycin. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
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    Effects of IFA (400 μg/mL) combined with antibiotics on bacterial viability of E. coli O157:H7. (A) <t>Fosfomycin</t> sodium. (B) Ceftriaxone. (C) Gentamicin. (D) Polymyxin B. (E) Streptomycin. (F) Chloramphenicol. (G) Tetracycline. (H) Azithromycin. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
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    ( A ) Phase contrast images of C. crescentus wild-type, Δ anmK (PR252), and Δ amgK (PR262) cells, harvested in the exponential and stationary growth phase. Scale bar: 5 µm. ( B ) Superplots showing the distribution of cell lengths in populations of C. crescentus wild-type, Δ anmK (PR252), and Δ amgK (PR262) cells in the exponential (exp) and stationary (stat) growth phase. Data (n=100 cells per replicate) are presented as described for . The statistical significance ( p -value) of differences between conditions was assessed using a two-sided Welch’s t -test. ns indicates p -values >0.1. ( C ) Serial dilution spot assay investigating the growth of C. crescentus Δ anmK (PR252) and Δ amgK (PR262) on plates containing different concentrations of ampicillin. The cells were spotted on the same plates as those depicted in . See for all replicates (n=3 independent experiments). ( D ) Levels of N-acetylmuramic acid (Mur N Ac) in the cytoplasm of C. crescentus wild-type and Δ amgK (PR262) cells, measured by metabolomics analysis through quantification of the corresponding mass spectrometric peak areas. The mass spectrometric peak areas were normalized against the mean obtained for the Δ amgK mutant. The statistical significance of differences between the two strains was determined using a two-sided Welch’s t -test. ( E ) Analysis of the growth of C. crescentus wild-type, Δ nagK (PR255), and Δ amgK (PR262) cells on agar containing a <t>fosfomycin</t> gradient. The fosfomycin concentrations on the <t>MIC</t> test strip are indicated in the legend on the right.
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    fosfomycin mic test stripes  (Liofilchem)
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    ( A ) Phase contrast images of C. crescentus wild-type, Δ anmK (PR252), and Δ amgK (PR262) cells, harvested in the exponential and stationary growth phase. Scale bar: 5 µm. ( B ) Superplots showing the distribution of cell lengths in populations of C. crescentus wild-type, Δ anmK (PR252), and Δ amgK (PR262) cells in the exponential (exp) and stationary (stat) growth phase. Data (n=100 cells per replicate) are presented as described for . The statistical significance ( p -value) of differences between conditions was assessed using a two-sided Welch’s t -test. ns indicates p -values >0.1. ( C ) Serial dilution spot assay investigating the growth of C. crescentus Δ anmK (PR252) and Δ amgK (PR262) on plates containing different concentrations of ampicillin. The cells were spotted on the same plates as those depicted in . See for all replicates (n=3 independent experiments). ( D ) Levels of N-acetylmuramic acid (Mur N Ac) in the cytoplasm of C. crescentus wild-type and Δ amgK (PR262) cells, measured by metabolomics analysis through quantification of the corresponding mass spectrometric peak areas. The mass spectrometric peak areas were normalized against the mean obtained for the Δ amgK mutant. The statistical significance of differences between the two strains was determined using a two-sided Welch’s t -test. ( E ) Analysis of the growth of C. crescentus wild-type, Δ nagK (PR255), and Δ amgK (PR262) cells on agar containing a <t>fosfomycin</t> gradient. The fosfomycin concentrations on the <t>MIC</t> test strip are indicated in the legend on the right.
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    ( A ) Phase contrast images of C. crescentus wild-type, Δ anmK (PR252), and Δ amgK (PR262) cells, harvested in the exponential and stationary growth phase. Scale bar: 5 µm. ( B ) Superplots showing the distribution of cell lengths in populations of C. crescentus wild-type, Δ anmK (PR252), and Δ amgK (PR262) cells in the exponential (exp) and stationary (stat) growth phase. Data (n=100 cells per replicate) are presented as described for . The statistical significance ( p -value) of differences between conditions was assessed using a two-sided Welch’s t -test. ns indicates p -values >0.1. ( C ) Serial dilution spot assay investigating the growth of C. crescentus Δ anmK (PR252) and Δ amgK (PR262) on plates containing different concentrations of ampicillin. The cells were spotted on the same plates as those depicted in . See for all replicates (n=3 independent experiments). ( D ) Levels of N-acetylmuramic acid (Mur N Ac) in the cytoplasm of C. crescentus wild-type and Δ amgK (PR262) cells, measured by metabolomics analysis through quantification of the corresponding mass spectrometric peak areas. The mass spectrometric peak areas were normalized against the mean obtained for the Δ amgK mutant. The statistical significance of differences between the two strains was determined using a two-sided Welch’s t -test. ( E ) Analysis of the growth of C. crescentus wild-type, Δ nagK (PR255), and Δ amgK (PR262) cells on agar containing a <t>fosfomycin</t> gradient. The fosfomycin concentrations on the <t>MIC</t> test strip are indicated in the legend on the right.
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    ( A ) Phase contrast images of C. crescentus wild-type, Δ anmK (PR252), and Δ amgK (PR262) cells, harvested in the exponential and stationary growth phase. Scale bar: 5 µm. ( B ) Superplots showing the distribution of cell lengths in populations of C. crescentus wild-type, Δ anmK (PR252), and Δ amgK (PR262) cells in the exponential (exp) and stationary (stat) growth phase. Data (n=100 cells per replicate) are presented as described for . The statistical significance ( p -value) of differences between conditions was assessed using a two-sided Welch’s t -test. ns indicates p -values >0.1. ( C ) Serial dilution spot assay investigating the growth of C. crescentus Δ anmK (PR252) and Δ amgK (PR262) on plates containing different concentrations of ampicillin. The cells were spotted on the same plates as those depicted in . See for all replicates (n=3 independent experiments). ( D ) Levels of N-acetylmuramic acid (Mur N Ac) in the cytoplasm of C. crescentus wild-type and Δ amgK (PR262) cells, measured by metabolomics analysis through quantification of the corresponding mass spectrometric peak areas. The mass spectrometric peak areas were normalized against the mean obtained for the Δ amgK mutant. The statistical significance of differences between the two strains was determined using a two-sided Welch’s t -test. ( E ) Analysis of the growth of C. crescentus wild-type, Δ nagK (PR255), and Δ amgK (PR262) cells on agar containing a <t>fosfomycin</t> gradient. The fosfomycin concentrations on the <t>MIC</t> test strip are indicated in the legend on the right.
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    ( A ) Phase contrast images of C. crescentus wild-type, Δ anmK (PR252), and Δ amgK (PR262) cells, harvested in the exponential and stationary growth phase. Scale bar: 5 µm. ( B ) Superplots showing the distribution of cell lengths in populations of C. crescentus wild-type, Δ anmK (PR252), and Δ amgK (PR262) cells in the exponential (exp) and stationary (stat) growth phase. Data (n=100 cells per replicate) are presented as described for . The statistical significance ( p -value) of differences between conditions was assessed using a two-sided Welch’s t -test. ns indicates p -values >0.1. ( C ) Serial dilution spot assay investigating the growth of C. crescentus Δ anmK (PR252) and Δ amgK (PR262) on plates containing different concentrations of ampicillin. The cells were spotted on the same plates as those depicted in . See for all replicates (n=3 independent experiments). ( D ) Levels of N-acetylmuramic acid (Mur N Ac) in the cytoplasm of C. crescentus wild-type and Δ amgK (PR262) cells, measured by metabolomics analysis through quantification of the corresponding mass spectrometric peak areas. The mass spectrometric peak areas were normalized against the mean obtained for the Δ amgK mutant. The statistical significance of differences between the two strains was determined using a two-sided Welch’s t -test. ( E ) Analysis of the growth of C. crescentus wild-type, Δ nagK (PR255), and Δ amgK (PR262) cells on agar containing a <t>fosfomycin</t> gradient. The fosfomycin concentrations on the <t>MIC</t> test strip are indicated in the legend on the right.
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    fosfomycin  (Liofilchem)
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    ( A ) Phase contrast images of C. crescentus wild-type, Δ anmK (PR252), and Δ amgK (PR262) cells, harvested in the exponential and stationary growth phase. Scale bar: 5 µm. ( B ) Superplots showing the distribution of cell lengths in populations of C. crescentus wild-type, Δ anmK (PR252), and Δ amgK (PR262) cells in the exponential (exp) and stationary (stat) growth phase. Data (n=100 cells per replicate) are presented as described for . The statistical significance ( p -value) of differences between conditions was assessed using a two-sided Welch’s t -test. ns indicates p -values >0.1. ( C ) Serial dilution spot assay investigating the growth of C. crescentus Δ anmK (PR252) and Δ amgK (PR262) on plates containing different concentrations of ampicillin. The cells were spotted on the same plates as those depicted in . See for all replicates (n=3 independent experiments). ( D ) Levels of N-acetylmuramic acid (Mur N Ac) in the cytoplasm of C. crescentus wild-type and Δ amgK (PR262) cells, measured by metabolomics analysis through quantification of the corresponding mass spectrometric peak areas. The mass spectrometric peak areas were normalized against the mean obtained for the Δ amgK mutant. The statistical significance of differences between the two strains was determined using a two-sided Welch’s t -test. ( E ) Analysis of the growth of C. crescentus wild-type, Δ nagK (PR255), and Δ amgK (PR262) cells on agar containing a <t>fosfomycin</t> gradient. The fosfomycin concentrations on the <t>MIC</t> test strip are indicated in the legend on the right.
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    Image Search Results


    Effects of IFA (400 μg/mL) combined with antibiotics on bacterial viability of E. coli O157:H7. (A) Fosfomycin sodium. (B) Ceftriaxone. (C) Gentamicin. (D) Polymyxin B. (E) Streptomycin. (F) Chloramphenicol. (G) Tetracycline. (H) Azithromycin. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Journal: Frontiers in Microbiology

    Article Title: Isoferulic acid suppresses Escherichia coli biofilm formation via LuxS/AI-2 quorum sensing inhibition and synergizes with fosfomycin

    doi: 10.3389/fmicb.2026.1837128

    Figure Lengend Snippet: Effects of IFA (400 μg/mL) combined with antibiotics on bacterial viability of E. coli O157:H7. (A) Fosfomycin sodium. (B) Ceftriaxone. (C) Gentamicin. (D) Polymyxin B. (E) Streptomycin. (F) Chloramphenicol. (G) Tetracycline. (H) Azithromycin. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Article Snippet: Fosfomycin sodium, cefquinome, gentamicin, polymyxin B, streptomycin, tetracycline, chloramphenicol, and azithromycin were purchased from MCE.

    Techniques:

    In vivo evaluation of the IFA/fosfomycin sodium combination strategy. (A) Mouse survival rate during the treatment period. (B) Bacterial load in ileum after different treatments. (C) Bacterial load in liver after different treatments. (D) Bacterial load in spleen after different treatments. (E) H&E staining of liver, spleen, and ileum tissues. Yellow arrows: shedding of intestinal villous epithelial cells, green arrows: cellular swelling, blue arrows: lymphocytes, purple arrows: granulocytes, black arrows: congestion, orange arrows: nuclear fragmentation, light green arrows: pigment deposition. (F) Fluorescence staining of tight junction proteins (ZO-1 and Occludin) in ileum tissues. **** p < 0.0001.

    Journal: Frontiers in Microbiology

    Article Title: Isoferulic acid suppresses Escherichia coli biofilm formation via LuxS/AI-2 quorum sensing inhibition and synergizes with fosfomycin

    doi: 10.3389/fmicb.2026.1837128

    Figure Lengend Snippet: In vivo evaluation of the IFA/fosfomycin sodium combination strategy. (A) Mouse survival rate during the treatment period. (B) Bacterial load in ileum after different treatments. (C) Bacterial load in liver after different treatments. (D) Bacterial load in spleen after different treatments. (E) H&E staining of liver, spleen, and ileum tissues. Yellow arrows: shedding of intestinal villous epithelial cells, green arrows: cellular swelling, blue arrows: lymphocytes, purple arrows: granulocytes, black arrows: congestion, orange arrows: nuclear fragmentation, light green arrows: pigment deposition. (F) Fluorescence staining of tight junction proteins (ZO-1 and Occludin) in ileum tissues. **** p < 0.0001.

    Article Snippet: Fosfomycin sodium, cefquinome, gentamicin, polymyxin B, streptomycin, tetracycline, chloramphenicol, and azithromycin were purchased from MCE.

    Techniques: In Vivo, Staining, Fluorescence

    ( A ) Phase contrast images of C. crescentus wild-type, Δ anmK (PR252), and Δ amgK (PR262) cells, harvested in the exponential and stationary growth phase. Scale bar: 5 µm. ( B ) Superplots showing the distribution of cell lengths in populations of C. crescentus wild-type, Δ anmK (PR252), and Δ amgK (PR262) cells in the exponential (exp) and stationary (stat) growth phase. Data (n=100 cells per replicate) are presented as described for . The statistical significance ( p -value) of differences between conditions was assessed using a two-sided Welch’s t -test. ns indicates p -values >0.1. ( C ) Serial dilution spot assay investigating the growth of C. crescentus Δ anmK (PR252) and Δ amgK (PR262) on plates containing different concentrations of ampicillin. The cells were spotted on the same plates as those depicted in . See for all replicates (n=3 independent experiments). ( D ) Levels of N-acetylmuramic acid (Mur N Ac) in the cytoplasm of C. crescentus wild-type and Δ amgK (PR262) cells, measured by metabolomics analysis through quantification of the corresponding mass spectrometric peak areas. The mass spectrometric peak areas were normalized against the mean obtained for the Δ amgK mutant. The statistical significance of differences between the two strains was determined using a two-sided Welch’s t -test. ( E ) Analysis of the growth of C. crescentus wild-type, Δ nagK (PR255), and Δ amgK (PR262) cells on agar containing a fosfomycin gradient. The fosfomycin concentrations on the MIC test strip are indicated in the legend on the right.

    Journal: eLife

    Article Title: Peptidoglycan recycling is critical for cell division, cell wall integrity, and β-lactam resistance in Caulobacter crescentus

    doi: 10.7554/eLife.109465

    Figure Lengend Snippet: ( A ) Phase contrast images of C. crescentus wild-type, Δ anmK (PR252), and Δ amgK (PR262) cells, harvested in the exponential and stationary growth phase. Scale bar: 5 µm. ( B ) Superplots showing the distribution of cell lengths in populations of C. crescentus wild-type, Δ anmK (PR252), and Δ amgK (PR262) cells in the exponential (exp) and stationary (stat) growth phase. Data (n=100 cells per replicate) are presented as described for . The statistical significance ( p -value) of differences between conditions was assessed using a two-sided Welch’s t -test. ns indicates p -values >0.1. ( C ) Serial dilution spot assay investigating the growth of C. crescentus Δ anmK (PR252) and Δ amgK (PR262) on plates containing different concentrations of ampicillin. The cells were spotted on the same plates as those depicted in . See for all replicates (n=3 independent experiments). ( D ) Levels of N-acetylmuramic acid (Mur N Ac) in the cytoplasm of C. crescentus wild-type and Δ amgK (PR262) cells, measured by metabolomics analysis through quantification of the corresponding mass spectrometric peak areas. The mass spectrometric peak areas were normalized against the mean obtained for the Δ amgK mutant. The statistical significance of differences between the two strains was determined using a two-sided Welch’s t -test. ( E ) Analysis of the growth of C. crescentus wild-type, Δ nagK (PR255), and Δ amgK (PR262) cells on agar containing a fosfomycin gradient. The fosfomycin concentrations on the MIC test strip are indicated in the legend on the right.

    Article Snippet: Resistance against fosfomycin was assessed using Fosfomycin MIC Test Strips (Liofilchem, Italy).

    Techniques: Serial Dilution, Spot Test, Mutagenesis, Stripping Membranes

    ( A ) Phase contrast images of C. crescentus wild-type, Δ anmK (PR252), and Δ amgK (PR262) cells, harvested in the exponential and stationary growth phase. Scale bar: 5 µm. ( B ) Superplots showing the distribution of cell lengths in populations of C. crescentus wild-type, Δ anmK (PR252), and Δ amgK (PR262) cells in the exponential (exp) and stationary (stat) growth phase. Data (n=100 cells per replicate) are presented as described for . The statistical significance ( p -value) of differences between conditions was assessed using a two-sided Welch’s t -test. ns indicates p -values >0.1. ( C ) Serial dilution spot assay investigating the growth of C. crescentus Δ anmK (PR252) and Δ amgK (PR262) on plates containing different concentrations of ampicillin. The cells were spotted on the same plates as those depicted in . See for all replicates (n=3 independent experiments). ( D ) Levels of N-acetylmuramic acid (Mur N Ac) in the cytoplasm of C. crescentus wild-type and Δ amgK (PR262) cells, measured by metabolomics analysis through quantification of the corresponding mass spectrometric peak areas. The mass spectrometric peak areas were normalized against the mean obtained for the Δ amgK mutant. The statistical significance of differences between the two strains was determined using a two-sided Welch’s t -test. ( E ) Analysis of the growth of C. crescentus wild-type, Δ nagK (PR255), and Δ amgK (PR262) cells on agar containing a fosfomycin gradient. The fosfomycin concentrations on the MIC test strip are indicated in the legend on the right.

    Journal: eLife

    Article Title: Peptidoglycan recycling is critical for cell division, cell wall integrity, and β-lactam resistance in Caulobacter crescentus

    doi: 10.7554/eLife.109465

    Figure Lengend Snippet: ( A ) Phase contrast images of C. crescentus wild-type, Δ anmK (PR252), and Δ amgK (PR262) cells, harvested in the exponential and stationary growth phase. Scale bar: 5 µm. ( B ) Superplots showing the distribution of cell lengths in populations of C. crescentus wild-type, Δ anmK (PR252), and Δ amgK (PR262) cells in the exponential (exp) and stationary (stat) growth phase. Data (n=100 cells per replicate) are presented as described for . The statistical significance ( p -value) of differences between conditions was assessed using a two-sided Welch’s t -test. ns indicates p -values >0.1. ( C ) Serial dilution spot assay investigating the growth of C. crescentus Δ anmK (PR252) and Δ amgK (PR262) on plates containing different concentrations of ampicillin. The cells were spotted on the same plates as those depicted in . See for all replicates (n=3 independent experiments). ( D ) Levels of N-acetylmuramic acid (Mur N Ac) in the cytoplasm of C. crescentus wild-type and Δ amgK (PR262) cells, measured by metabolomics analysis through quantification of the corresponding mass spectrometric peak areas. The mass spectrometric peak areas were normalized against the mean obtained for the Δ amgK mutant. The statistical significance of differences between the two strains was determined using a two-sided Welch’s t -test. ( E ) Analysis of the growth of C. crescentus wild-type, Δ nagK (PR255), and Δ amgK (PR262) cells on agar containing a fosfomycin gradient. The fosfomycin concentrations on the MIC test strip are indicated in the legend on the right.

    Article Snippet: Chemical compound, drug , fosfomycin MIC test stripes , Liofilchem, Italy , Cat. #: 92078 , .

    Techniques: Serial Dilution, Spot Test, Mutagenesis, Stripping Membranes