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fosfomycin mic test strips  (Liofilchem)

 
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    Liofilchem fosfomycin mic test strips
    ( A ) Phase contrast images of C. crescentus wild-type, Δ anmK (PR252), and Δ amgK (PR262) cells, harvested in the exponential and stationary growth phase. Scale bar: 5 µm. ( B ) Superplots showing the distribution of cell lengths in populations of C. crescentus wild-type, Δ anmK (PR252), and Δ amgK (PR262) cells in the exponential (exp) and stationary (stat) growth phase. Data (n=100 cells per replicate) are presented as described for . The statistical significance ( p -value) of differences between conditions was assessed using a two-sided Welch’s t -test. ns indicates p -values >0.1. ( C ) Serial dilution spot assay investigating the growth of C. crescentus Δ anmK (PR252) and Δ amgK (PR262) on plates containing different concentrations of ampicillin. The cells were spotted on the same plates as those depicted in . See for all replicates (n=3 independent experiments). ( D ) Levels of N-acetylmuramic acid (Mur N Ac) in the cytoplasm of C. crescentus wild-type and Δ amgK (PR262) cells, measured by metabolomics analysis through quantification of the corresponding mass spectrometric peak areas. The mass spectrometric peak areas were normalized against the mean obtained for the Δ amgK mutant. The statistical significance of differences between the two strains was determined using a two-sided Welch’s t -test. ( E ) Analysis of the growth of C. crescentus wild-type, Δ nagK (PR255), and Δ amgK (PR262) cells on agar containing a <t>fosfomycin</t> gradient. The fosfomycin concentrations on the <t>MIC</t> test strip are indicated in the legend on the right.
    Fosfomycin Mic Test Strips, supplied by Liofilchem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphonomycin/mic+strip+test+%D0%BE%D0%BF%D1%80%D0%B5%D0%B4%D0%B5%D0%BB%D1%8F%D0%BB%D0%B8+%D0%BF%D0%BE%D0%BC%D0%BE%D1%89%D1%8C%D1%8E+%D1%81/pmc13046382-377-6-10
    Average 86 stars, based on 1 article reviews
    fosfomycin mic test strips - by Bioz Stars, 2026-11
    86/100 stars

    Images

    1) Product Images from "Peptidoglycan recycling is critical for cell division, cell wall integrity, and β-lactam resistance in Caulobacter crescentus"

    Article Title: Peptidoglycan recycling is critical for cell division, cell wall integrity, and β-lactam resistance in Caulobacter crescentus

    Journal: eLife

    doi: 10.7554/eLife.109465

    ( A ) Phase contrast images of C. crescentus wild-type, Δ anmK (PR252), and Δ amgK (PR262) cells, harvested in the exponential and stationary growth phase. Scale bar: 5 µm. ( B ) Superplots showing the distribution of cell lengths in populations of C. crescentus wild-type, Δ anmK (PR252), and Δ amgK (PR262) cells in the exponential (exp) and stationary (stat) growth phase. Data (n=100 cells per replicate) are presented as described for . The statistical significance ( p -value) of differences between conditions was assessed using a two-sided Welch’s t -test. ns indicates p -values >0.1. ( C ) Serial dilution spot assay investigating the growth of C. crescentus Δ anmK (PR252) and Δ amgK (PR262) on plates containing different concentrations of ampicillin. The cells were spotted on the same plates as those depicted in . See for all replicates (n=3 independent experiments). ( D ) Levels of N-acetylmuramic acid (Mur N Ac) in the cytoplasm of C. crescentus wild-type and Δ amgK (PR262) cells, measured by metabolomics analysis through quantification of the corresponding mass spectrometric peak areas. The mass spectrometric peak areas were normalized against the mean obtained for the Δ amgK mutant. The statistical significance of differences between the two strains was determined using a two-sided Welch’s t -test. ( E ) Analysis of the growth of C. crescentus wild-type, Δ nagK (PR255), and Δ amgK (PR262) cells on agar containing a fosfomycin gradient. The fosfomycin concentrations on the MIC test strip are indicated in the legend on the right.
    Figure Legend Snippet: ( A ) Phase contrast images of C. crescentus wild-type, Δ anmK (PR252), and Δ amgK (PR262) cells, harvested in the exponential and stationary growth phase. Scale bar: 5 µm. ( B ) Superplots showing the distribution of cell lengths in populations of C. crescentus wild-type, Δ anmK (PR252), and Δ amgK (PR262) cells in the exponential (exp) and stationary (stat) growth phase. Data (n=100 cells per replicate) are presented as described for . The statistical significance ( p -value) of differences between conditions was assessed using a two-sided Welch’s t -test. ns indicates p -values >0.1. ( C ) Serial dilution spot assay investigating the growth of C. crescentus Δ anmK (PR252) and Δ amgK (PR262) on plates containing different concentrations of ampicillin. The cells were spotted on the same plates as those depicted in . See for all replicates (n=3 independent experiments). ( D ) Levels of N-acetylmuramic acid (Mur N Ac) in the cytoplasm of C. crescentus wild-type and Δ amgK (PR262) cells, measured by metabolomics analysis through quantification of the corresponding mass spectrometric peak areas. The mass spectrometric peak areas were normalized against the mean obtained for the Δ amgK mutant. The statistical significance of differences between the two strains was determined using a two-sided Welch’s t -test. ( E ) Analysis of the growth of C. crescentus wild-type, Δ nagK (PR255), and Δ amgK (PR262) cells on agar containing a fosfomycin gradient. The fosfomycin concentrations on the MIC test strip are indicated in the legend on the right.

    Techniques Used: Serial Dilution, Spot Test, Mutagenesis, Stripping Membranes

    Related Articles

    other:

    Article Title: Comprehensive Evaluation of Safety and Probiotic Potential of Lactiplantibacillus plantarum HY7715 Isolated from Korean kimchi.
    Article Snippet: dermatologic, oral, and mental health conditions [8–10].. However, probiotic effects are strain–specific and context–dependent.. Moreover, probiotic use may be critical in vulnerable populations, such as preterm infants, immunocompromised patients, and critically ill individuals, as rare cases have been reported of probiotic–associated bacteremia or sepsis [11, 12].

    Article Title: Fluoroquinolone resistance-conferring gyrA variants alter the fitness cost and potentiate the resistance of the zoliflodacin resistance mutation gyrB D429N in Neisseria gonorrhoeae
    Article Snippet: Antibiotic susceptibility testing was performed on GCB-K agar via MIC test strips (Bio-Merieux and Liofilchem) for ciprofloxacin or agar dilution for zoliflodacin, gepotidacin, and nalidixic acid.

    Article Title: β-lactamase genes in clinical isolates of Capnocytophaga canimorsus and description of a novel class D β-lactamase, OXA-1422.
    Article Snippet: Minimum inhibitory concentrations (MICs) of penicillin, amoxicillin, piperacillin-tazobactam, oxacillin, cefoxitin, cefotaxime, ceftazidime, aztreonam, ertapenem, imipenem, and meropenem were determined using ETEST strips (bioMérieux, Marcy l’Etoile, France).


    Mutagenesis:

    Article Title: Glycerol-Driven Energy and Proteostasis Underpin Antibiotic Tolerance in Escherichia coli
    Article Snippet: .. The minimum inhibitory concentrations (MICs) of antibiotics for WT and mutant strains were determined using Liofilchem MTS (MIC Test Strips) (Fisher Scientific, Atlanta, GA). ..

    Stripping Membranes:

    Article Title: Exploiting outer-membrane protein promiscuity to induce transient collateral sensitivity via efflux pump competition
    Article Snippet: .. MICs of ciprofloxacin and tobramycin were determined at 37 oC, in Mueller Hinton (MH) agar, using E-test strips (MIC Test Strip, Liofilchem®) in the presence or absence of 10 μg/mL of DC, as previously described . ..

    Article Title: The IL-17A/Neutrophil axis play a critical role in lethal infection induced by an emerging ultra-virulent Streptococcus suis serotype 5 strain.
    Article Snippet: .. To determine whether the AR genes in genomes conferred the predicted resistance to the corresponding bacteria, we used the MIC-test strip (Liofilchem, Roseto degli Abruzzi, Italy) to assess the antimicrobial susceptibility of clindamycin (Cat. No. 920721, 0.016-256μg/mL), erythromycin (Cat. No. 920511, 0.016-256μg/mL), azithromycin (Cat. No. 920301, 0.016-256μg/mL), tetracycline (Cat. No. 921141, 0.016-256μg/mL), cefotaxime (Cat. No. 920431, 0.016-256μg/mL), vancomycin (Cat. No. 920571, 0.016-256μg/mL), penicillin (Cat. No. 921021, 0.016-256μg/mL), and spectinomycin (Cat. No. 920141, 0.016-1024μg/mL) according to the methods and resistance breakpoints previously described [9]. ..

    Bacteria:

    Article Title: The IL-17A/Neutrophil axis play a critical role in lethal infection induced by an emerging ultra-virulent Streptococcus suis serotype 5 strain.
    Article Snippet: .. To determine whether the AR genes in genomes conferred the predicted resistance to the corresponding bacteria, we used the MIC-test strip (Liofilchem, Roseto degli Abruzzi, Italy) to assess the antimicrobial susceptibility of clindamycin (Cat. No. 920721, 0.016-256μg/mL), erythromycin (Cat. No. 920511, 0.016-256μg/mL), azithromycin (Cat. No. 920301, 0.016-256μg/mL), tetracycline (Cat. No. 921141, 0.016-256μg/mL), cefotaxime (Cat. No. 920431, 0.016-256μg/mL), vancomycin (Cat. No. 920571, 0.016-256μg/mL), penicillin (Cat. No. 921021, 0.016-256μg/mL), and spectinomycin (Cat. No. 920141, 0.016-1024μg/mL) according to the methods and resistance breakpoints previously described [9]. ..



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    ( A ) Phase contrast images of C. crescentus wild-type, Δ anmK (PR252), and Δ amgK (PR262) cells, harvested in the exponential and stationary growth phase. Scale bar: 5 µm. ( B ) Superplots showing the distribution of cell lengths in populations of C. crescentus wild-type, Δ anmK (PR252), and Δ amgK (PR262) cells in the exponential (exp) and stationary (stat) growth phase. Data (n=100 cells per replicate) are presented as described for . The statistical significance ( p -value) of differences between conditions was assessed using a two-sided Welch’s t -test. ns indicates p -values >0.1. ( C ) Serial dilution spot assay investigating the growth of C. crescentus Δ anmK (PR252) and Δ amgK (PR262) on plates containing different concentrations of ampicillin. The cells were spotted on the same plates as those depicted in . See for all replicates (n=3 independent experiments). ( D ) Levels of N-acetylmuramic acid (Mur N Ac) in the cytoplasm of C. crescentus wild-type and Δ amgK (PR262) cells, measured by metabolomics analysis through quantification of the corresponding mass spectrometric peak areas. The mass spectrometric peak areas were normalized against the mean obtained for the Δ amgK mutant. The statistical significance of differences between the two strains was determined using a two-sided Welch’s t -test. ( E ) Analysis of the growth of C. crescentus wild-type, Δ nagK (PR255), and Δ amgK (PR262) cells on agar containing a <t>fosfomycin</t> gradient. The fosfomycin concentrations on the <t>MIC</t> test strip are indicated in the legend on the right.
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    ( A ) Phase contrast images of C. crescentus wild-type, Δ anmK (PR252), and Δ amgK (PR262) cells, harvested in the exponential and stationary growth phase. Scale bar: 5 µm. ( B ) Superplots showing the distribution of cell lengths in populations of C. crescentus wild-type, Δ anmK (PR252), and Δ amgK (PR262) cells in the exponential (exp) and stationary (stat) growth phase. Data (n=100 cells per replicate) are presented as described for . The statistical significance ( p -value) of differences between conditions was assessed using a two-sided Welch’s t -test. ns indicates p -values >0.1. ( C ) Serial dilution spot assay investigating the growth of C. crescentus Δ anmK (PR252) and Δ amgK (PR262) on plates containing different concentrations of ampicillin. The cells were spotted on the same plates as those depicted in . See for all replicates (n=3 independent experiments). ( D ) Levels of N-acetylmuramic acid (Mur N Ac) in the cytoplasm of C. crescentus wild-type and Δ amgK (PR262) cells, measured by metabolomics analysis through quantification of the corresponding mass spectrometric peak areas. The mass spectrometric peak areas were normalized against the mean obtained for the Δ amgK mutant. The statistical significance of differences between the two strains was determined using a two-sided Welch’s t -test. ( E ) Analysis of the growth of C. crescentus wild-type, Δ nagK (PR255), and Δ amgK (PR262) cells on agar containing a <t>fosfomycin</t> gradient. The fosfomycin concentrations on the <t>MIC</t> test strip are indicated in the legend on the right.
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    ( A ) Phase contrast images of C. crescentus wild-type, Δ anmK (PR252), and Δ amgK (PR262) cells, harvested in the exponential and stationary growth phase. Scale bar: 5 µm. ( B ) Superplots showing the distribution of cell lengths in populations of C. crescentus wild-type, Δ anmK (PR252), and Δ amgK (PR262) cells in the exponential (exp) and stationary (stat) growth phase. Data (n=100 cells per replicate) are presented as described for . The statistical significance ( p -value) of differences between conditions was assessed using a two-sided Welch’s t -test. ns indicates p -values >0.1. ( C ) Serial dilution spot assay investigating the growth of C. crescentus Δ anmK (PR252) and Δ amgK (PR262) on plates containing different concentrations of ampicillin. The cells were spotted on the same plates as those depicted in . See for all replicates (n=3 independent experiments). ( D ) Levels of N-acetylmuramic acid (Mur N Ac) in the cytoplasm of C. crescentus wild-type and Δ amgK (PR262) cells, measured by metabolomics analysis through quantification of the corresponding mass spectrometric peak areas. The mass spectrometric peak areas were normalized against the mean obtained for the Δ amgK mutant. The statistical significance of differences between the two strains was determined using a two-sided Welch’s t -test. ( E ) Analysis of the growth of C. crescentus wild-type, Δ nagK (PR255), and Δ amgK (PR262) cells on agar containing a <t>fosfomycin</t> gradient. The fosfomycin concentrations on the <t>MIC</t> test strip are indicated in the legend on the right.
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    ( A ) Phase contrast images of C. crescentus wild-type, Δ anmK (PR252), and Δ amgK (PR262) cells, harvested in the exponential and stationary growth phase. Scale bar: 5 µm. ( B ) Superplots showing the distribution of cell lengths in populations of C. crescentus wild-type, Δ anmK (PR252), and Δ amgK (PR262) cells in the exponential (exp) and stationary (stat) growth phase. Data (n=100 cells per replicate) are presented as described for . The statistical significance ( p -value) of differences between conditions was assessed using a two-sided Welch’s t -test. ns indicates p -values >0.1. ( C ) Serial dilution spot assay investigating the growth of C. crescentus Δ anmK (PR252) and Δ amgK (PR262) on plates containing different concentrations of ampicillin. The cells were spotted on the same plates as those depicted in . See for all replicates (n=3 independent experiments). ( D ) Levels of N-acetylmuramic acid (Mur N Ac) in the cytoplasm of C. crescentus wild-type and Δ amgK (PR262) cells, measured by metabolomics analysis through quantification of the corresponding mass spectrometric peak areas. The mass spectrometric peak areas were normalized against the mean obtained for the Δ amgK mutant. The statistical significance of differences between the two strains was determined using a two-sided Welch’s t -test. ( E ) Analysis of the growth of C. crescentus wild-type, Δ nagK (PR255), and Δ amgK (PR262) cells on agar containing a <t>fosfomycin</t> gradient. The fosfomycin concentrations on the <t>MIC</t> test strip are indicated in the legend on the right.
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    Image Search Results


    Effects of IFA (400 μg/mL) combined with antibiotics on bacterial viability of E. coli O157:H7. (A) Fosfomycin sodium. (B) Ceftriaxone. (C) Gentamicin. (D) Polymyxin B. (E) Streptomycin. (F) Chloramphenicol. (G) Tetracycline. (H) Azithromycin. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Journal: Frontiers in Microbiology

    Article Title: Isoferulic acid suppresses Escherichia coli biofilm formation via LuxS/AI-2 quorum sensing inhibition and synergizes with fosfomycin

    doi: 10.3389/fmicb.2026.1837128

    Figure Lengend Snippet: Effects of IFA (400 μg/mL) combined with antibiotics on bacterial viability of E. coli O157:H7. (A) Fosfomycin sodium. (B) Ceftriaxone. (C) Gentamicin. (D) Polymyxin B. (E) Streptomycin. (F) Chloramphenicol. (G) Tetracycline. (H) Azithromycin. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Article Snippet: Fosfomycin sodium, cefquinome, gentamicin, polymyxin B, streptomycin, tetracycline, chloramphenicol, and azithromycin were purchased from MCE.

    Techniques:

    In vivo evaluation of the IFA/fosfomycin sodium combination strategy. (A) Mouse survival rate during the treatment period. (B) Bacterial load in ileum after different treatments. (C) Bacterial load in liver after different treatments. (D) Bacterial load in spleen after different treatments. (E) H&E staining of liver, spleen, and ileum tissues. Yellow arrows: shedding of intestinal villous epithelial cells, green arrows: cellular swelling, blue arrows: lymphocytes, purple arrows: granulocytes, black arrows: congestion, orange arrows: nuclear fragmentation, light green arrows: pigment deposition. (F) Fluorescence staining of tight junction proteins (ZO-1 and Occludin) in ileum tissues. **** p < 0.0001.

    Journal: Frontiers in Microbiology

    Article Title: Isoferulic acid suppresses Escherichia coli biofilm formation via LuxS/AI-2 quorum sensing inhibition and synergizes with fosfomycin

    doi: 10.3389/fmicb.2026.1837128

    Figure Lengend Snippet: In vivo evaluation of the IFA/fosfomycin sodium combination strategy. (A) Mouse survival rate during the treatment period. (B) Bacterial load in ileum after different treatments. (C) Bacterial load in liver after different treatments. (D) Bacterial load in spleen after different treatments. (E) H&E staining of liver, spleen, and ileum tissues. Yellow arrows: shedding of intestinal villous epithelial cells, green arrows: cellular swelling, blue arrows: lymphocytes, purple arrows: granulocytes, black arrows: congestion, orange arrows: nuclear fragmentation, light green arrows: pigment deposition. (F) Fluorescence staining of tight junction proteins (ZO-1 and Occludin) in ileum tissues. **** p < 0.0001.

    Article Snippet: Fosfomycin sodium, cefquinome, gentamicin, polymyxin B, streptomycin, tetracycline, chloramphenicol, and azithromycin were purchased from MCE.

    Techniques: In Vivo, Staining, Fluorescence

    ( A ) Phase contrast images of C. crescentus wild-type, Δ anmK (PR252), and Δ amgK (PR262) cells, harvested in the exponential and stationary growth phase. Scale bar: 5 µm. ( B ) Superplots showing the distribution of cell lengths in populations of C. crescentus wild-type, Δ anmK (PR252), and Δ amgK (PR262) cells in the exponential (exp) and stationary (stat) growth phase. Data (n=100 cells per replicate) are presented as described for . The statistical significance ( p -value) of differences between conditions was assessed using a two-sided Welch’s t -test. ns indicates p -values >0.1. ( C ) Serial dilution spot assay investigating the growth of C. crescentus Δ anmK (PR252) and Δ amgK (PR262) on plates containing different concentrations of ampicillin. The cells were spotted on the same plates as those depicted in . See for all replicates (n=3 independent experiments). ( D ) Levels of N-acetylmuramic acid (Mur N Ac) in the cytoplasm of C. crescentus wild-type and Δ amgK (PR262) cells, measured by metabolomics analysis through quantification of the corresponding mass spectrometric peak areas. The mass spectrometric peak areas were normalized against the mean obtained for the Δ amgK mutant. The statistical significance of differences between the two strains was determined using a two-sided Welch’s t -test. ( E ) Analysis of the growth of C. crescentus wild-type, Δ nagK (PR255), and Δ amgK (PR262) cells on agar containing a fosfomycin gradient. The fosfomycin concentrations on the MIC test strip are indicated in the legend on the right.

    Journal: eLife

    Article Title: Peptidoglycan recycling is critical for cell division, cell wall integrity, and β-lactam resistance in Caulobacter crescentus

    doi: 10.7554/eLife.109465

    Figure Lengend Snippet: ( A ) Phase contrast images of C. crescentus wild-type, Δ anmK (PR252), and Δ amgK (PR262) cells, harvested in the exponential and stationary growth phase. Scale bar: 5 µm. ( B ) Superplots showing the distribution of cell lengths in populations of C. crescentus wild-type, Δ anmK (PR252), and Δ amgK (PR262) cells in the exponential (exp) and stationary (stat) growth phase. Data (n=100 cells per replicate) are presented as described for . The statistical significance ( p -value) of differences between conditions was assessed using a two-sided Welch’s t -test. ns indicates p -values >0.1. ( C ) Serial dilution spot assay investigating the growth of C. crescentus Δ anmK (PR252) and Δ amgK (PR262) on plates containing different concentrations of ampicillin. The cells were spotted on the same plates as those depicted in . See for all replicates (n=3 independent experiments). ( D ) Levels of N-acetylmuramic acid (Mur N Ac) in the cytoplasm of C. crescentus wild-type and Δ amgK (PR262) cells, measured by metabolomics analysis through quantification of the corresponding mass spectrometric peak areas. The mass spectrometric peak areas were normalized against the mean obtained for the Δ amgK mutant. The statistical significance of differences between the two strains was determined using a two-sided Welch’s t -test. ( E ) Analysis of the growth of C. crescentus wild-type, Δ nagK (PR255), and Δ amgK (PR262) cells on agar containing a fosfomycin gradient. The fosfomycin concentrations on the MIC test strip are indicated in the legend on the right.

    Article Snippet: Resistance against fosfomycin was assessed using Fosfomycin MIC Test Strips (Liofilchem, Italy).

    Techniques: Serial Dilution, Spot Test, Mutagenesis, Stripping Membranes

    ( A ) Phase contrast images of C. crescentus wild-type, Δ anmK (PR252), and Δ amgK (PR262) cells, harvested in the exponential and stationary growth phase. Scale bar: 5 µm. ( B ) Superplots showing the distribution of cell lengths in populations of C. crescentus wild-type, Δ anmK (PR252), and Δ amgK (PR262) cells in the exponential (exp) and stationary (stat) growth phase. Data (n=100 cells per replicate) are presented as described for . The statistical significance ( p -value) of differences between conditions was assessed using a two-sided Welch’s t -test. ns indicates p -values >0.1. ( C ) Serial dilution spot assay investigating the growth of C. crescentus Δ anmK (PR252) and Δ amgK (PR262) on plates containing different concentrations of ampicillin. The cells were spotted on the same plates as those depicted in . See for all replicates (n=3 independent experiments). ( D ) Levels of N-acetylmuramic acid (Mur N Ac) in the cytoplasm of C. crescentus wild-type and Δ amgK (PR262) cells, measured by metabolomics analysis through quantification of the corresponding mass spectrometric peak areas. The mass spectrometric peak areas were normalized against the mean obtained for the Δ amgK mutant. The statistical significance of differences between the two strains was determined using a two-sided Welch’s t -test. ( E ) Analysis of the growth of C. crescentus wild-type, Δ nagK (PR255), and Δ amgK (PR262) cells on agar containing a fosfomycin gradient. The fosfomycin concentrations on the MIC test strip are indicated in the legend on the right.

    Journal: eLife

    Article Title: Peptidoglycan recycling is critical for cell division, cell wall integrity, and β-lactam resistance in Caulobacter crescentus

    doi: 10.7554/eLife.109465

    Figure Lengend Snippet: ( A ) Phase contrast images of C. crescentus wild-type, Δ anmK (PR252), and Δ amgK (PR262) cells, harvested in the exponential and stationary growth phase. Scale bar: 5 µm. ( B ) Superplots showing the distribution of cell lengths in populations of C. crescentus wild-type, Δ anmK (PR252), and Δ amgK (PR262) cells in the exponential (exp) and stationary (stat) growth phase. Data (n=100 cells per replicate) are presented as described for . The statistical significance ( p -value) of differences between conditions was assessed using a two-sided Welch’s t -test. ns indicates p -values >0.1. ( C ) Serial dilution spot assay investigating the growth of C. crescentus Δ anmK (PR252) and Δ amgK (PR262) on plates containing different concentrations of ampicillin. The cells were spotted on the same plates as those depicted in . See for all replicates (n=3 independent experiments). ( D ) Levels of N-acetylmuramic acid (Mur N Ac) in the cytoplasm of C. crescentus wild-type and Δ amgK (PR262) cells, measured by metabolomics analysis through quantification of the corresponding mass spectrometric peak areas. The mass spectrometric peak areas were normalized against the mean obtained for the Δ amgK mutant. The statistical significance of differences between the two strains was determined using a two-sided Welch’s t -test. ( E ) Analysis of the growth of C. crescentus wild-type, Δ nagK (PR255), and Δ amgK (PR262) cells on agar containing a fosfomycin gradient. The fosfomycin concentrations on the MIC test strip are indicated in the legend on the right.

    Article Snippet: Chemical compound, drug , fosfomycin MIC test stripes , Liofilchem, Italy , Cat. #: 92078 , .

    Techniques: Serial Dilution, Spot Test, Mutagenesis, Stripping Membranes