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kam-880 microarray kit  (Kinex Pharmaceuticals)

 
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    Kinex Pharmaceuticals kam-880 microarray kit
    FOXO1 expression is reduced in HSC-5 NW cells. ( A ) Individual duplicate arrays of phospho-Ser319 FOXO1 for the HSC-5 CN (red) and HSC-5 NW (green) cell lysate protein microarrays; the complete Kinex <t>KAM-880</t> microarray is shown in . The average values of HSC-5 CN and HSC-5 NW cell phospho-Ser319 FOXO1 arrays are shown. ( B ) Representative Western blots of FOXO1 and GAPDH in HSC-5 CN and HSC-5 NW cells; n = 3. ( C ) Densitometric quantification of the FOXO1/GAPDH ratio in HSC-5 CN and HSC-5 NW cells normalized to HSC-5 CN cells. ( D ) Total RNA was extracted from HSC-5 CN and HSC-5 NW cells and reverse-transcribed to cDNA for quantifying FOXO1 expression relative to MRPL-27 using real-time PCR, normalized to HSC-5 CN cells. ( E ) Representative Western blots of FOXO1 and GAPDH in HSC-5 CN and HSC-5 NW cells treated with 10 µM MG132 or DMSO; n = 3. ( F ) Densitometric quantification of the FOXO1/GAPDH ratio in HSC-5 CN and HSC-5 NW cells treated as in ( E ) normalized to DMSO-treated HSC-5 CN cells. ( G ) Representative immunofluorescence images of HSC-5 CN and HSC-5 NW cells treated as in ( E ) stained for FOXO1 (green). Nuclei were counterstained with DAPI (blue). Scale bar represents 20 µm, n = 3. All values are the mean ± SD, n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001.
    Kam 880 Microarray Kit, supplied by Kinex Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phospho-specific+protein+microarray+analysis/kam+880+antibody+microarray/pmc09029996-119-14-13
    Average 90 stars, based on 1 article reviews
    kam-880 microarray kit - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "N-WASP Attenuates Cell Proliferation and Migration through ERK2-Dependent Enhanced Expression of TXNIP"

    Article Title: N-WASP Attenuates Cell Proliferation and Migration through ERK2-Dependent Enhanced Expression of TXNIP

    Journal: Biology

    doi: 10.3390/biology11040582

    FOXO1 expression is reduced in HSC-5 NW cells. ( A ) Individual duplicate arrays of phospho-Ser319 FOXO1 for the HSC-5 CN (red) and HSC-5 NW (green) cell lysate protein microarrays; the complete Kinex KAM-880 microarray is shown in . The average values of HSC-5 CN and HSC-5 NW cell phospho-Ser319 FOXO1 arrays are shown. ( B ) Representative Western blots of FOXO1 and GAPDH in HSC-5 CN and HSC-5 NW cells; n = 3. ( C ) Densitometric quantification of the FOXO1/GAPDH ratio in HSC-5 CN and HSC-5 NW cells normalized to HSC-5 CN cells. ( D ) Total RNA was extracted from HSC-5 CN and HSC-5 NW cells and reverse-transcribed to cDNA for quantifying FOXO1 expression relative to MRPL-27 using real-time PCR, normalized to HSC-5 CN cells. ( E ) Representative Western blots of FOXO1 and GAPDH in HSC-5 CN and HSC-5 NW cells treated with 10 µM MG132 or DMSO; n = 3. ( F ) Densitometric quantification of the FOXO1/GAPDH ratio in HSC-5 CN and HSC-5 NW cells treated as in ( E ) normalized to DMSO-treated HSC-5 CN cells. ( G ) Representative immunofluorescence images of HSC-5 CN and HSC-5 NW cells treated as in ( E ) stained for FOXO1 (green). Nuclei were counterstained with DAPI (blue). Scale bar represents 20 µm, n = 3. All values are the mean ± SD, n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001.
    Figure Legend Snippet: FOXO1 expression is reduced in HSC-5 NW cells. ( A ) Individual duplicate arrays of phospho-Ser319 FOXO1 for the HSC-5 CN (red) and HSC-5 NW (green) cell lysate protein microarrays; the complete Kinex KAM-880 microarray is shown in . The average values of HSC-5 CN and HSC-5 NW cell phospho-Ser319 FOXO1 arrays are shown. ( B ) Representative Western blots of FOXO1 and GAPDH in HSC-5 CN and HSC-5 NW cells; n = 3. ( C ) Densitometric quantification of the FOXO1/GAPDH ratio in HSC-5 CN and HSC-5 NW cells normalized to HSC-5 CN cells. ( D ) Total RNA was extracted from HSC-5 CN and HSC-5 NW cells and reverse-transcribed to cDNA for quantifying FOXO1 expression relative to MRPL-27 using real-time PCR, normalized to HSC-5 CN cells. ( E ) Representative Western blots of FOXO1 and GAPDH in HSC-5 CN and HSC-5 NW cells treated with 10 µM MG132 or DMSO; n = 3. ( F ) Densitometric quantification of the FOXO1/GAPDH ratio in HSC-5 CN and HSC-5 NW cells treated as in ( E ) normalized to DMSO-treated HSC-5 CN cells. ( G ) Representative immunofluorescence images of HSC-5 CN and HSC-5 NW cells treated as in ( E ) stained for FOXO1 (green). Nuclei were counterstained with DAPI (blue). Scale bar represents 20 µm, n = 3. All values are the mean ± SD, n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001.

    Techniques Used: Expressing, Microarray, Western Blot, Real-time Polymerase Chain Reaction, Immunofluorescence, Staining

    Related Articles

    Activation Assay:

    Article Title: Hepatitis A Virus Cellular Receptor 1 (HAVcr-1) Initiates Prostate Cancer Progression in Human Cells via Hepatocyte Growth Factor (HGF)-Induced Changes in Junctional Integrity
    Article Snippet: .. There has been some research to indicate that HGF is important in HAVcR-1 activation and, therefore, it would be interesting to investigate the effect of a combination of HGF and HAVcR-1 on cell behaviours and TJ integrity [ ] The Kinex™ KAM880 protein array analysis indicated 64 proteins as being statistically altered, either in expression or phosphorylation. ..

    Protein Array:

    Article Title: Hepatitis A Virus Cellular Receptor 1 (HAVcr-1) Initiates Prostate Cancer Progression in Human Cells via Hepatocyte Growth Factor (HGF)-Induced Changes in Junctional Integrity
    Article Snippet: .. There has been some research to indicate that HGF is important in HAVcR-1 activation and, therefore, it would be interesting to investigate the effect of a combination of HGF and HAVcR-1 on cell behaviours and TJ integrity [ ] The Kinex™ KAM880 protein array analysis indicated 64 proteins as being statistically altered, either in expression or phosphorylation. ..

    Expressing:

    Article Title: Hepatitis A Virus Cellular Receptor 1 (HAVcr-1) Initiates Prostate Cancer Progression in Human Cells via Hepatocyte Growth Factor (HGF)-Induced Changes in Junctional Integrity
    Article Snippet: .. There has been some research to indicate that HGF is important in HAVcR-1 activation and, therefore, it would be interesting to investigate the effect of a combination of HGF and HAVcR-1 on cell behaviours and TJ integrity [ ] The Kinex™ KAM880 protein array analysis indicated 64 proteins as being statistically altered, either in expression or phosphorylation. ..

    other:

    Article Title: Hepatitis A Virus Cellular Receptor 1 (HAVcr-1) Initiates Prostate Cancer Progression in Human Cells via Hepatocyte Growth Factor (HGF)-Induced Changes in Junctional Integrity
    Article Snippet: These results, therefore, support the Kinex™ KAM880 protein array and the theory that HAVcR-1 could lead to the phosphorylation of α-catenin, which, subsequently, results in the disassociation of β-catenin from AJs and nuclear accumulation.

    Article Title: Tretinoin synergistically enhances the antitumor effect of combined BRAF, MEK, and EGFR inhibition in BRAF V600E colorectal cancer.
    Article Snippet: Next, to elucidate whether the addition of TRE to ENC/BIN affected the expression or phosphorylation of specific proteins, we used the Kinex antibody microarray KAM- 2000 to analyze the expression of total proteins including unphosphorylated and phosphorylated proteins, and phosphorylated proteins by RKO cells with each treatment (Table S13).

    Article Title: N-WASP Attenuates Cell Proliferation and Migration through ERK2-Dependent Enhanced Expression of TXNIP
    Article Snippet: To identify the signaling pathways altered in HSC-5 NW cells, we used the Kinex KAM-880 microarray kit, which detects 518 pan-specific proteins and 359 phospho-specific proteins critical for cell signaling pathways.

    Article Title: Tretinoin synergistically enhances the antitumor effect of combined BRAF, MEK, and EGFR inhibition in BRAF V600E colorectal cancer
    Article Snippet: Next, to elucidate whether the addition of TRE to ENC/BIN affected the expression or phosphorylation of specific proteins, we used the Kinex antibody microarray KAM‐2000 to analyze the expression of total proteins including unphosphorylated and phosphorylated proteins, and phosphorylated proteins by RKO cells with each treatment (Table ).

    Article Title: EPLIN, a Putative Tumour Suppressor in Colorectal Cancer, Implications in Drug Resistance
    Article Snippet: Kinex TM KAM-880 antibody-based protein microarray (Kinexus Bioinformatics Ltd., Vancouver, BC, Canada) was performed on two pairs of patient’s protein (normal: ID126 & 128; tumour: ID127 & 129) to investigate potential interacting partners.

    Article Title: Hepatitis A Virus Cellular Receptor 1 (HAVcr-1) Initiates Prostate Cancer Progression in Human Cells via Hepatocyte Growth Factor (HGF)-Induced Changes in Junctional Integrity
    Article Snippet: We found an increase in the β-catenin nuclear staining, and since nuclear staining is indicative of β-catenin activation, this, therefore, supports the Kinex™ KAM880 protein array data and the theory that HAVcR-1 is involved in β-catenin signalling [ ].

    Article Title: Deletion of Tac1 gene impact kinase phosphorylation involved in signaling pathways associated with pain
    Article Snippet: We performed phosphoproteomics using the Kinex KAM-850 antibody microarray. displays up- and downregulated site-specific phosphoproteins (i.e., Z-ratio ≥ 1.2 and % error ≤ 30) using a heatmap feature.



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    Image Search Results


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    Techniques: Phospho-proteomics, Control, Expressing