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human mtor signalling phospho-specific antibody microarray  (Full Moon BioSystems)

 
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    Full Moon BioSystems human mtor signalling phospho-specific antibody microarray
    Dysregulation of the <t>mTOR</t> signaling pathway. (A) . Heat map representation of phosphoprotein ratio detected in full moon Biosystems mTOR phospho-protein array. Fold change was calculated after normalizing the average signal cor-responding to the median signal for each group, and the ratio has been calculated as KO vs. WT signals. Proteins that have shown at least 2-fold up or downregulation were included. (B) . KO cells show less co-localization of mTOR (green) with the lysosome (LAMP1, red) in refed and starved conditions than WT cortical cells. Scale bar represents 10 um. (C,D) The total intensity of mTOR and LAMP1 was measured with cell profiler using immunostaining images in WT (C) and (D) KO. Graphs represent mean ± SEM; * p < 0.05, ** p < 0.01, Student’s t-test ( n = 15–17 images for each condition). (E–G) Data representation of the total mean of intensity of (E) mTOR and (F) LAMP1 in an area of interest in the cytoplasm (doughnut) of WT cells at different conditions, respectively. Whispers max and min plot represent total mTOR and total LAMP1 intensity in the cell. (G) mTOR and LAMP1 correlation in basal, starved, and refed conditions. The graph rep-resents Pearson’s co-efficient correlation between LAMP1 and total mTOR in WT and KO cells. (H–L) Bar graph represents changed upstream protein phosphorylation changes at basal (H) , starved (J) , and refed (L) conditions. Further repre-sentation of phosphorylated target of downstream regulators altered at basal (I) , starved (K) , and refed conditions (M) . (N,O) Further targeted protein changed from the transition from basal to starved upstream regulators (N) and downstream regulators (O) . (I,K) represents affected proteins between trans ion from starved to refed, upstream regulators (P) and downstream regulators (Q) .
    Human Mtor Signalling Phospho Specific Antibody Microarray, supplied by Full Moon BioSystems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phospho-specific+protein+microarray+analysis/mtor+phospho+antibody+microarray/pmc09017388-255-0-9
    Average 90 stars, based on 1 article reviews
    human mtor signalling phospho-specific antibody microarray - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "SLC38A10 Regulate Glutamate Homeostasis and Modulate the AKT/TSC2/mTOR Pathway in Mouse Primary Cortex Cells"

    Article Title: SLC38A10 Regulate Glutamate Homeostasis and Modulate the AKT/TSC2/mTOR Pathway in Mouse Primary Cortex Cells

    Journal: Frontiers in Cell and Developmental Biology

    doi: 10.3389/fcell.2022.854397

    Dysregulation of the mTOR signaling pathway. (A) . Heat map representation of phosphoprotein ratio detected in full moon Biosystems mTOR phospho-protein array. Fold change was calculated after normalizing the average signal cor-responding to the median signal for each group, and the ratio has been calculated as KO vs. WT signals. Proteins that have shown at least 2-fold up or downregulation were included. (B) . KO cells show less co-localization of mTOR (green) with the lysosome (LAMP1, red) in refed and starved conditions than WT cortical cells. Scale bar represents 10 um. (C,D) The total intensity of mTOR and LAMP1 was measured with cell profiler using immunostaining images in WT (C) and (D) KO. Graphs represent mean ± SEM; * p < 0.05, ** p < 0.01, Student’s t-test ( n = 15–17 images for each condition). (E–G) Data representation of the total mean of intensity of (E) mTOR and (F) LAMP1 in an area of interest in the cytoplasm (doughnut) of WT cells at different conditions, respectively. Whispers max and min plot represent total mTOR and total LAMP1 intensity in the cell. (G) mTOR and LAMP1 correlation in basal, starved, and refed conditions. The graph rep-resents Pearson’s co-efficient correlation between LAMP1 and total mTOR in WT and KO cells. (H–L) Bar graph represents changed upstream protein phosphorylation changes at basal (H) , starved (J) , and refed (L) conditions. Further repre-sentation of phosphorylated target of downstream regulators altered at basal (I) , starved (K) , and refed conditions (M) . (N,O) Further targeted protein changed from the transition from basal to starved upstream regulators (N) and downstream regulators (O) . (I,K) represents affected proteins between trans ion from starved to refed, upstream regulators (P) and downstream regulators (Q) .
    Figure Legend Snippet: Dysregulation of the mTOR signaling pathway. (A) . Heat map representation of phosphoprotein ratio detected in full moon Biosystems mTOR phospho-protein array. Fold change was calculated after normalizing the average signal cor-responding to the median signal for each group, and the ratio has been calculated as KO vs. WT signals. Proteins that have shown at least 2-fold up or downregulation were included. (B) . KO cells show less co-localization of mTOR (green) with the lysosome (LAMP1, red) in refed and starved conditions than WT cortical cells. Scale bar represents 10 um. (C,D) The total intensity of mTOR and LAMP1 was measured with cell profiler using immunostaining images in WT (C) and (D) KO. Graphs represent mean ± SEM; * p < 0.05, ** p < 0.01, Student’s t-test ( n = 15–17 images for each condition). (E–G) Data representation of the total mean of intensity of (E) mTOR and (F) LAMP1 in an area of interest in the cytoplasm (doughnut) of WT cells at different conditions, respectively. Whispers max and min plot represent total mTOR and total LAMP1 intensity in the cell. (G) mTOR and LAMP1 correlation in basal, starved, and refed conditions. The graph rep-resents Pearson’s co-efficient correlation between LAMP1 and total mTOR in WT and KO cells. (H–L) Bar graph represents changed upstream protein phosphorylation changes at basal (H) , starved (J) , and refed (L) conditions. Further repre-sentation of phosphorylated target of downstream regulators altered at basal (I) , starved (K) , and refed conditions (M) . (N,O) Further targeted protein changed from the transition from basal to starved upstream regulators (N) and downstream regulators (O) . (I,K) represents affected proteins between trans ion from starved to refed, upstream regulators (P) and downstream regulators (Q) .

    Techniques Used: Protein Array, Immunostaining, Phospho-proteomics

    The response in mTOR pathway and amino acid regualtion following SLC38A10 knockout. Complete transcriptomic data and changed gene list subjected to IPA software analysis. (A) Regulation in the mTOR pathway in response to SLC38A10 knockout in Basal condition, blue symbols indicate down regulation and red symbols up regulation. (B) Heatmap describing changes in transcript levels from the mTOR pathway during Basal, Starved and Refed conditions. (C) Heatmap describing changes in transcript levels from the p70S6 pathway during Basal, Starved and Refed conditions. (D) Regulation in the mTOR pathway in response to SLC38A10 knockout in Starved condition, blue symbols indicate down regulation and red symbols up regulation. (E) Regulation in the mTOR pathway in response to SLC38A10 knockout in Refed condition, blue symbols indicate down regulation and red symbols up regulation. (F) Neurological diseases predicted by Path Designer, in response to SLC38A10 knockout.
    Figure Legend Snippet: The response in mTOR pathway and amino acid regualtion following SLC38A10 knockout. Complete transcriptomic data and changed gene list subjected to IPA software analysis. (A) Regulation in the mTOR pathway in response to SLC38A10 knockout in Basal condition, blue symbols indicate down regulation and red symbols up regulation. (B) Heatmap describing changes in transcript levels from the mTOR pathway during Basal, Starved and Refed conditions. (C) Heatmap describing changes in transcript levels from the p70S6 pathway during Basal, Starved and Refed conditions. (D) Regulation in the mTOR pathway in response to SLC38A10 knockout in Starved condition, blue symbols indicate down regulation and red symbols up regulation. (E) Regulation in the mTOR pathway in response to SLC38A10 knockout in Refed condition, blue symbols indicate down regulation and red symbols up regulation. (F) Neurological diseases predicted by Path Designer, in response to SLC38A10 knockout.

    Techniques Used: Knock-Out, Software

    Related Articles

    other:

    Article Title: SLC38A10 Regulate Glutamate Homeostasis and Modulate the AKT/TSC2/mTOR Pathway in Mouse Primary Cortex Cells
    Article Snippet: Human mTOR signalling Phospho-Specific Antibody Microarray was purchased from Full moon Biosystems.

    Article Title: Engineered extracellular vesicles antagonize SARS-CoV-2 infection by inhibiting mTOR signaling.
    Article Snippet: TOR phospho-antibody microarray Postranscriptional effects of ASTEX on SARS-CoV-2-infected Calucells was performed using the mTOR phospho-antibody microarray Full Moon Biosystems; cat# PMT138).

    Article Title: Engineered extracellular vesicles antagonize SARS-CoV-2 infection by inhibiting mTOR signaling
    Article Snippet: Postranscriptional effects of ASTEX on SARS-CoV-2-infected Calu-3 cells was performed using the mTOR phospho-antibody microarray (Full Moon Biosystems; cat# PMT138).

    Ab Array:

    Article Title: Mitochondrial pyruvate carrier inhibition initiates metabolic crosstalk to stimulate branched chain amino acid catabolism.
    Article Snippet: .. The following day, cells were starved in DMEM containing no serum, glucose, or amino acids with indicated treatments for 2 h then samples used for the mTOR Phospho Antibody Array (Full Moon Biosystems PMT138) according to manufacturer’s instructions. ..

    Article Title: Resf1 is a compound G4 quadruplex-associated tumor suppressor for triple negative breast cancer.
    Article Snippet: .. Subnuclear fractionation assays were performed with a Thermo Scientific Subcellular Fractionation Kit for Cultured Cells (cat. No. 78840), following the manufacturers suggested protocol. mTOR pathway analysis mTOR pathway analysis was performed with the Full Moon Biosystems mTOR Phospho Antibody array (cat. No. PMT138) using shScramble and shG5 MDA-MB-231 cell lysates, following the manufacturers recommended protocol. ..

    Article Title: Resf1 is a compound G4 quadruplex-associated tumor suppressor for triple negative breast cancer
    Article Snippet: Subnuclear fractionation assays were performed with a Thermo Scientific Subcellular Fractionation Kit for Cultured Cells (cat. No. 78840), following the manufacturers suggested protocol. .. mTOR pathway analysis was performed with the Full Moon Biosystems mTOR Phospho Antibody array (cat. No. PMT138) using shScramble and shG5 MDA-MB-231 cell lysates, following the manufacturers recommended protocol. ..


    Fractionation:

    Article Title: Resf1 is a compound G4 quadruplex-associated tumor suppressor for triple negative breast cancer.
    Article Snippet: .. Subnuclear fractionation assays were performed with a Thermo Scientific Subcellular Fractionation Kit for Cultured Cells (cat. No. 78840), following the manufacturers suggested protocol. mTOR pathway analysis mTOR pathway analysis was performed with the Full Moon Biosystems mTOR Phospho Antibody array (cat. No. PMT138) using shScramble and shG5 MDA-MB-231 cell lysates, following the manufacturers recommended protocol. ..

    Cell Culture:

    Article Title: Resf1 is a compound G4 quadruplex-associated tumor suppressor for triple negative breast cancer.
    Article Snippet: .. Subnuclear fractionation assays were performed with a Thermo Scientific Subcellular Fractionation Kit for Cultured Cells (cat. No. 78840), following the manufacturers suggested protocol. mTOR pathway analysis mTOR pathway analysis was performed with the Full Moon Biosystems mTOR Phospho Antibody array (cat. No. PMT138) using shScramble and shG5 MDA-MB-231 cell lysates, following the manufacturers recommended protocol. ..

    Multiple Displacement Amplification:

    Article Title: Resf1 is a compound G4 quadruplex-associated tumor suppressor for triple negative breast cancer.
    Article Snippet: .. Subnuclear fractionation assays were performed with a Thermo Scientific Subcellular Fractionation Kit for Cultured Cells (cat. No. 78840), following the manufacturers suggested protocol. mTOR pathway analysis mTOR pathway analysis was performed with the Full Moon Biosystems mTOR Phospho Antibody array (cat. No. PMT138) using shScramble and shG5 MDA-MB-231 cell lysates, following the manufacturers recommended protocol. ..

    Article Title: Resf1 is a compound G4 quadruplex-associated tumor suppressor for triple negative breast cancer
    Article Snippet: Subnuclear fractionation assays were performed with a Thermo Scientific Subcellular Fractionation Kit for Cultured Cells (cat. No. 78840), following the manufacturers suggested protocol. .. mTOR pathway analysis was performed with the Full Moon Biosystems mTOR Phospho Antibody array (cat. No. PMT138) using shScramble and shG5 MDA-MB-231 cell lysates, following the manufacturers recommended protocol. ..



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    Image Search Results


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