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phosphate buffered saline  (Thermo Fisher)


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    Structured Review

    Thermo Fisher phosphate buffered saline
    Phosphate Buffered Saline, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphate+buffer/Phosphate-buffered+saline+(PBS%2C+1X)%2C+sterile-filtered/pmc12907503-219-34-43
    Average 99 stars, based on 1 article reviews
    phosphate buffered saline - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Saline:

    Article Title: Multiplexed Detection of Membranous Nephropathy Antigens by Multi-Reaction Monitoring Mass Spectrometry
    Article Snippet: .. Frozen kidney biopsy tissue remnants were washed in phosphate buffered saline, followed by bead-beating in 400 μl Pierce IP lysis buffer (Thermo Scientific #87787) containing protease and phosphatase inhibitors (Halt protease/phosphatase inhibitor at 1:100 dilution, Thermo Scientific #87786) to produce protein extracts. .. Protein extracts were immunoprecipitated with protein A/G beads (Pierce #88803) in the presence of 50 ng stable isotope-labeled universal monoclonal antibody (SILuMab K1;Sigma-Aldrich #MSQC6), and 50 ng SILuMab K4 (Sigma-Aldrich #MSQC7).

    Article Title: AdMSC spheroids encapsulating antioxidant hybrid protein carrier for irradiation-damaged salivary gland repair
    Article Snippet: To evaluate changes in secondary protein structure, CD spectra were acquired using a circular dichroism spectropolarimeter (J-815; Jasco, Japan). .. Gel and GG were each prepared at a concentration of 0.1 mg mL −1 in 1X phosphate-buffered saline (PBS, pH 7.4, Thermo Fisher Scientific). ..

    Article Title: Early nasal and lung transcriptomic profiles reveal pathways associated with divergent clinical outcomes following H7N1 high pathogenicity avian influenza virus infection
    Article Snippet: Whole blood samples were preserved in TRIzol Reagent (Invitrogen). .. Swabs were placed in 1 ml of sterile phosphate-buffered saline (PBS) containing 1% penicillin-streptomycin (Thermo Fisher Scientific) and nystatin (Sigma-Aldrich). ..

    Article Title: Obtention of conditional media from Wharton’s gelatin mesenchymal stromal cells in a dynamic culture system
    Article Snippet: .. Luego, los cordones se transportaron al laboratorio, debidamente refrigerados e inmersos en una solución salina tampón (Phosphate Buffered Saline, PBS) (PBS 1X, GIBCO), con suplemento al 1 % de antibiótico y antimicótico (Sigma Aldrich Inc.). ..

    Article Title: Collagen II hydrogel-mediated sustained delivery of lacosamide attenuates cartilage degeneration and pain in osteoarthritis
    Article Snippet: .. Their initial weight (W 0 ), was recorded, and they were then immersed in 1x phosphate-buffered saline (PBS, Gibco) at a constant temperature of 37 °C. ..

    Article Title: Polyplex of peptide-mannan and RNA for intranasal delivery of TGF-β siRNA in treatment of pulmonary fibrosis
    Article Snippet: GAPDH reverse and forward primers were purchased from Integrated DNA Technologies, Inc (Coralville, IA). .. Zeba Spin Desalting Columns (7k MOCO), RiboGreen RNA Assay Kit, PowerUp SYBR Green Master Mix, High-Capacity cDNA Reverse Transcription Kit, DMEM/F-12, CHCA MALDI Matrix, and fetal bovine serum (FBS), Alexa Fluor 647 Dye (AF647), phosphate-buffered saline (PBS, pH = 7.4) were purchased from ThermoFisher Scientific (Waltham, MA). .. TGF-β siRNA and Cy5-labeled TGF-β siRNA were purchased from Horizon discovery (Cambridge, UK).

    Article Title: Synergistic Induction of Caspase-8-Mediated Leukaemic Cell Death by Fisetin and Pinocembrin.
    Article Snippet: .. Jurkat cells were seeded at a density of 1 × 105 cells/mL and treated with fisetin at concentrations of 5, 10, 15 and 20 μM for 48 h. After treatment, cells were collected, washed twice with cold 1X phosphate-buffered saline (PBS), and lysed in RIPA buffer (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 1X protease inhibitor cocktail (Thermo Fisher Scientific, Rockford, IL, USA) on ice for 15 min. .. The total protein concentrations were quantified using a bicinchoninic acid (BCA) protein assay (Thermo Scientific, Waltham, MA, USA).

    Lysis:

    Article Title: Multiplexed Detection of Membranous Nephropathy Antigens by Multi-Reaction Monitoring Mass Spectrometry
    Article Snippet: .. Frozen kidney biopsy tissue remnants were washed in phosphate buffered saline, followed by bead-beating in 400 μl Pierce IP lysis buffer (Thermo Scientific #87787) containing protease and phosphatase inhibitors (Halt protease/phosphatase inhibitor at 1:100 dilution, Thermo Scientific #87786) to produce protein extracts. .. Protein extracts were immunoprecipitated with protein A/G beads (Pierce #88803) in the presence of 50 ng stable isotope-labeled universal monoclonal antibody (SILuMab K1;Sigma-Aldrich #MSQC6), and 50 ng SILuMab K4 (Sigma-Aldrich #MSQC7).

    Concentration Assay:

    Article Title: AdMSC spheroids encapsulating antioxidant hybrid protein carrier for irradiation-damaged salivary gland repair
    Article Snippet: To evaluate changes in secondary protein structure, CD spectra were acquired using a circular dichroism spectropolarimeter (J-815; Jasco, Japan). .. Gel and GG were each prepared at a concentration of 0.1 mg mL −1 in 1X phosphate-buffered saline (PBS, pH 7.4, Thermo Fisher Scientific). ..

    Sterility:

    Article Title: Early nasal and lung transcriptomic profiles reveal pathways associated with divergent clinical outcomes following H7N1 high pathogenicity avian influenza virus infection
    Article Snippet: Whole blood samples were preserved in TRIzol Reagent (Invitrogen). .. Swabs were placed in 1 ml of sterile phosphate-buffered saline (PBS) containing 1% penicillin-streptomycin (Thermo Fisher Scientific) and nystatin (Sigma-Aldrich). ..

    Blocking Assay:

    Article Title: Hyperlipidemia induces hippocampal inflammation and loss of vascularity and can be rescued by silencing RIPK1.
    Article Snippet: Serial crosssections Ten μm-thick sections were sectioned with a Thermo Cryostat (Scientific #HM550). .. Slides were rinsed 3x with PBS 1x and permeabilized with PBS-T 5% (5% Triton-X in PBS) solution 3x for 5 min and block for 1.5 h in a humid slide chamber with blocking solution (10% normal donkey serum and 5% fish gelatin in PBS-T 5%), followed by primary antibodies in blocking solution for 24 hr at 4°C overnight, and finally with Alexa Fluor-conjugated secondary antibodies (1:500, Invitrogen) in blocking solution for 2h at room temperature in a humid slide chamber. .. Slides were mounted with aqueous mounting medium (Dako #s3023).

    SYBR Green Assay:

    Article Title: Polyplex of peptide-mannan and RNA for intranasal delivery of TGF-β siRNA in treatment of pulmonary fibrosis
    Article Snippet: GAPDH reverse and forward primers were purchased from Integrated DNA Technologies, Inc (Coralville, IA). .. Zeba Spin Desalting Columns (7k MOCO), RiboGreen RNA Assay Kit, PowerUp SYBR Green Master Mix, High-Capacity cDNA Reverse Transcription Kit, DMEM/F-12, CHCA MALDI Matrix, and fetal bovine serum (FBS), Alexa Fluor 647 Dye (AF647), phosphate-buffered saline (PBS, pH = 7.4) were purchased from ThermoFisher Scientific (Waltham, MA). .. TGF-β siRNA and Cy5-labeled TGF-β siRNA were purchased from Horizon discovery (Cambridge, UK).

    Reverse Transcription:

    Article Title: Polyplex of peptide-mannan and RNA for intranasal delivery of TGF-β siRNA in treatment of pulmonary fibrosis
    Article Snippet: GAPDH reverse and forward primers were purchased from Integrated DNA Technologies, Inc (Coralville, IA). .. Zeba Spin Desalting Columns (7k MOCO), RiboGreen RNA Assay Kit, PowerUp SYBR Green Master Mix, High-Capacity cDNA Reverse Transcription Kit, DMEM/F-12, CHCA MALDI Matrix, and fetal bovine serum (FBS), Alexa Fluor 647 Dye (AF647), phosphate-buffered saline (PBS, pH = 7.4) were purchased from ThermoFisher Scientific (Waltham, MA). .. TGF-β siRNA and Cy5-labeled TGF-β siRNA were purchased from Horizon discovery (Cambridge, UK).

    Protease Inhibitor:

    Article Title: Synergistic Induction of Caspase-8-Mediated Leukaemic Cell Death by Fisetin and Pinocembrin.
    Article Snippet: .. Jurkat cells were seeded at a density of 1 × 105 cells/mL and treated with fisetin at concentrations of 5, 10, 15 and 20 μM for 48 h. After treatment, cells were collected, washed twice with cold 1X phosphate-buffered saline (PBS), and lysed in RIPA buffer (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 1X protease inhibitor cocktail (Thermo Fisher Scientific, Rockford, IL, USA) on ice for 15 min. .. The total protein concentrations were quantified using a bicinchoninic acid (BCA) protein assay (Thermo Scientific, Waltham, MA, USA).



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    Methodological workflow from sample preparation to measuring the cellular response capacity (CRC). Following blood collection, samples are prepared for flow cytometric analysis either unstimulated <t>(PBS</t> as buffer control) or stimulated with an inflammatory cocktail (cocktail of N-formylmethionyl-leucyl-phenylalanine, platelet-activating factor, and tumor necrosis factor). The CRC is calculated as the ratio of median fluorescence intensity (MFI) between stimulated and unstimulated neutrophils. Three approaches, classic, simple, and kinetic CRC, offer distinct advantages and limitations based on technical aspects such as manual processing steps (e.g., centrifugation) and incubation time. n =13–14. Statistical analysis was performed using the Kruskal-Wallis test with Dunn’s post-hoc test, comparing patients with sepsis at all time points (shown in the figure: 0 h, not shown in the figure: 24, 72, 120 h) with healthy volunteers (HV). P -values are indicated above the respective data points. Asterisks indicate significant differences between HV and patients at 0 h only. ⁎ P <0.05, ⁎⁎ P <0.01, ⁎⁎⁎ P <0.001. AUC. Area under the curve; AU. Arbitrary units; <t>PBS.</t> <t>Phosphate-buffered</t> saline; E. coli Escherichia coli .
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    Nacalai phosphate buffered saline
    Characterization of extracellular vesicles (EVs) derived from conditioned medium (CM). (a) Particle size distribution and concentration of EVs measured by nanoparticle tracking analysis. Median particle diameter: 143.6 nm; particle concentration: 1.6 × 10 9 particles/mL per 1 mL of unpurified CM prior to EV isolation. (b) Zeta potential of EVs measured <t>in</t> <t>phosphate-buffered</t> saline, showing a negatively charged surface of −36.38 mV. (c) Quantification of CD63-positive EVs using a Tim4–CD63 sandwich enzyme-linked immunosorbent assay. EV concentration is expressed per 1 mL of pre-purified CM. Statistical analysis using Welch's t -test demonstrated a significant difference between CM and EV samples ( n = 3 per group, ∗ p < 0.001).
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    Thermo Fisher dulbecco s phosphate buffered saline dpbs
    Characterization of extracellular vesicles (EVs) derived from conditioned medium (CM). (a) Particle size distribution and concentration of EVs measured by nanoparticle tracking analysis. Median particle diameter: 143.6 nm; particle concentration: 1.6 × 10 9 particles/mL per 1 mL of unpurified CM prior to EV isolation. (b) Zeta potential of EVs measured <t>in</t> <t>phosphate-buffered</t> saline, showing a negatively charged surface of −36.38 mV. (c) Quantification of CD63-positive EVs using a Tim4–CD63 sandwich enzyme-linked immunosorbent assay. EV concentration is expressed per 1 mL of pre-purified CM. Statistical analysis using Welch's t -test demonstrated a significant difference between CM and EV samples ( n = 3 per group, ∗ p < 0.001).
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    Thermo Fisher dpbs
    Characterization of extracellular vesicles (EVs) derived from conditioned medium (CM). (a) Particle size distribution and concentration of EVs measured by nanoparticle tracking analysis. Median particle diameter: 143.6 nm; particle concentration: 1.6 × 10 9 particles/mL per 1 mL of unpurified CM prior to EV isolation. (b) Zeta potential of EVs measured <t>in</t> <t>phosphate-buffered</t> saline, showing a negatively charged surface of −36.38 mV. (c) Quantification of CD63-positive EVs using a Tim4–CD63 sandwich enzyme-linked immunosorbent assay. EV concentration is expressed per 1 mL of pre-purified CM. Statistical analysis using Welch's t -test demonstrated a significant difference between CM and EV samples ( n = 3 per group, ∗ p < 0.001).
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    Thermo Fisher 1x phosphate buffered saline
    Characterization of extracellular vesicles (EVs) derived from conditioned medium (CM). (a) Particle size distribution and concentration of EVs measured by nanoparticle tracking analysis. Median particle diameter: 143.6 nm; particle concentration: 1.6 × 10 9 particles/mL per 1 mL of unpurified CM prior to EV isolation. (b) Zeta potential of EVs measured <t>in</t> <t>phosphate-buffered</t> saline, showing a negatively charged surface of −36.38 mV. (c) Quantification of CD63-positive EVs using a Tim4–CD63 sandwich enzyme-linked immunosorbent assay. EV concentration is expressed per 1 mL of pre-purified CM. Statistical analysis using Welch's t -test demonstrated a significant difference between CM and EV samples ( n = 3 per group, ∗ p < 0.001).
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    Image Search Results


    Methodological workflow from sample preparation to measuring the cellular response capacity (CRC). Following blood collection, samples are prepared for flow cytometric analysis either unstimulated (PBS as buffer control) or stimulated with an inflammatory cocktail (cocktail of N-formylmethionyl-leucyl-phenylalanine, platelet-activating factor, and tumor necrosis factor). The CRC is calculated as the ratio of median fluorescence intensity (MFI) between stimulated and unstimulated neutrophils. Three approaches, classic, simple, and kinetic CRC, offer distinct advantages and limitations based on technical aspects such as manual processing steps (e.g., centrifugation) and incubation time. n =13–14. Statistical analysis was performed using the Kruskal-Wallis test with Dunn’s post-hoc test, comparing patients with sepsis at all time points (shown in the figure: 0 h, not shown in the figure: 24, 72, 120 h) with healthy volunteers (HV). P -values are indicated above the respective data points. Asterisks indicate significant differences between HV and patients at 0 h only. ⁎ P <0.05, ⁎⁎ P <0.01, ⁎⁎⁎ P <0.001. AUC. Area under the curve; AU. Arbitrary units; PBS. Phosphate-buffered saline; E. coli Escherichia coli .

    Journal: Military Medical Research

    Article Title: The cellular response capacity (CRC) as a novel immunomonitoring approach in sepsis

    doi: 10.1016/j.mmr.2026.100010

    Figure Lengend Snippet: Methodological workflow from sample preparation to measuring the cellular response capacity (CRC). Following blood collection, samples are prepared for flow cytometric analysis either unstimulated (PBS as buffer control) or stimulated with an inflammatory cocktail (cocktail of N-formylmethionyl-leucyl-phenylalanine, platelet-activating factor, and tumor necrosis factor). The CRC is calculated as the ratio of median fluorescence intensity (MFI) between stimulated and unstimulated neutrophils. Three approaches, classic, simple, and kinetic CRC, offer distinct advantages and limitations based on technical aspects such as manual processing steps (e.g., centrifugation) and incubation time. n =13–14. Statistical analysis was performed using the Kruskal-Wallis test with Dunn’s post-hoc test, comparing patients with sepsis at all time points (shown in the figure: 0 h, not shown in the figure: 24, 72, 120 h) with healthy volunteers (HV). P -values are indicated above the respective data points. Asterisks indicate significant differences between HV and patients at 0 h only. ⁎ P <0.05, ⁎⁎ P <0.01, ⁎⁎⁎ P <0.001. AUC. Area under the curve; AU. Arbitrary units; PBS. Phosphate-buffered saline; E. coli Escherichia coli .

    Article Snippet: Subsequently, the monovettes were exposed to either phosphate-buffered saline (PBS) with calcium and magnesium (PBS +/+ , #14080055, Gibco, Thermo Fisher Scientific, Waltham, USA) and the bacterial culture media [buffer control of Escherichia coli ( E. coli ) suspension, hereafter referred to as BuC], viable E. coli bacteria (ATCC line 25922, DSMZ, Braunschweig, Germany), or LPS (100 ng/ml, from E. coli O55:B5, #L2637, Sigma Aldrich, Steinheim, Germany).

    Techniques: Sample Prep, Control, Fluorescence, Centrifugation, Incubation, Saline

    Comparison of different approaches to measure the cellular response capacity (CRC) for CD11b on neutrophil granulocytes. Blood from healthy volunteers was incubated with either PBS (Control) or 100 ng/ml LPS for 60 min in the ex vivo whole blood model. a Analysis of the median fluorescence intensity (MFI). b Evaluation of the CRC as determined by different approaches (classic, simple, and kinetic CRC). c, d Change in fluorescence intensity and change in CRC using the kinetic CRC approach, comparing blood with previous exposure to LPS or PBS (buffer control) from the ex vivo whole blood model. In both c and d , the single-cell values measured by flow cytometry were condensed using a moving median with a window of 9 cells. This moving median was then approximated with a 5 th -degree polynomial function. The plots display these polynomial functions together with the baseline (median before stimulation) and a connecting line from the baseline to the polynomial function for 30 s after stimulation. Values are shown as median and interquartile range. n= 10. Statistical analysis was performed using the Mann-Whitney U test. ⁎⁎⁎ P <0.001. AU. Arbitrary units; LPS. Lipopolysaccharide; PBS. Phosphate-buffered saline.

    Journal: Military Medical Research

    Article Title: The cellular response capacity (CRC) as a novel immunomonitoring approach in sepsis

    doi: 10.1016/j.mmr.2026.100010

    Figure Lengend Snippet: Comparison of different approaches to measure the cellular response capacity (CRC) for CD11b on neutrophil granulocytes. Blood from healthy volunteers was incubated with either PBS (Control) or 100 ng/ml LPS for 60 min in the ex vivo whole blood model. a Analysis of the median fluorescence intensity (MFI). b Evaluation of the CRC as determined by different approaches (classic, simple, and kinetic CRC). c, d Change in fluorescence intensity and change in CRC using the kinetic CRC approach, comparing blood with previous exposure to LPS or PBS (buffer control) from the ex vivo whole blood model. In both c and d , the single-cell values measured by flow cytometry were condensed using a moving median with a window of 9 cells. This moving median was then approximated with a 5 th -degree polynomial function. The plots display these polynomial functions together with the baseline (median before stimulation) and a connecting line from the baseline to the polynomial function for 30 s after stimulation. Values are shown as median and interquartile range. n= 10. Statistical analysis was performed using the Mann-Whitney U test. ⁎⁎⁎ P <0.001. AU. Arbitrary units; LPS. Lipopolysaccharide; PBS. Phosphate-buffered saline.

    Article Snippet: Subsequently, the monovettes were exposed to either phosphate-buffered saline (PBS) with calcium and magnesium (PBS +/+ , #14080055, Gibco, Thermo Fisher Scientific, Waltham, USA) and the bacterial culture media [buffer control of Escherichia coli ( E. coli ) suspension, hereafter referred to as BuC], viable E. coli bacteria (ATCC line 25922, DSMZ, Braunschweig, Germany), or LPS (100 ng/ml, from E. coli O55:B5, #L2637, Sigma Aldrich, Steinheim, Germany).

    Techniques: Comparison, Incubation, Control, Ex Vivo, Fluorescence, Single Cell, Flow Cytometry, MANN-WHITNEY, Saline

    Characterization of extracellular vesicles (EVs) derived from conditioned medium (CM). (a) Particle size distribution and concentration of EVs measured by nanoparticle tracking analysis. Median particle diameter: 143.6 nm; particle concentration: 1.6 × 10 9 particles/mL per 1 mL of unpurified CM prior to EV isolation. (b) Zeta potential of EVs measured in phosphate-buffered saline, showing a negatively charged surface of −36.38 mV. (c) Quantification of CD63-positive EVs using a Tim4–CD63 sandwich enzyme-linked immunosorbent assay. EV concentration is expressed per 1 mL of pre-purified CM. Statistical analysis using Welch's t -test demonstrated a significant difference between CM and EV samples ( n = 3 per group, ∗ p < 0.001).

    Journal: Regenerative Therapy

    Article Title: Dental pulp cell–derived conditioned culture media promotes periodontal repair through immunomodulation and bone regeneration

    doi: 10.1016/j.reth.2026.101144

    Figure Lengend Snippet: Characterization of extracellular vesicles (EVs) derived from conditioned medium (CM). (a) Particle size distribution and concentration of EVs measured by nanoparticle tracking analysis. Median particle diameter: 143.6 nm; particle concentration: 1.6 × 10 9 particles/mL per 1 mL of unpurified CM prior to EV isolation. (b) Zeta potential of EVs measured in phosphate-buffered saline, showing a negatively charged surface of −36.38 mV. (c) Quantification of CD63-positive EVs using a Tim4–CD63 sandwich enzyme-linked immunosorbent assay. EV concentration is expressed per 1 mL of pre-purified CM. Statistical analysis using Welch's t -test demonstrated a significant difference between CM and EV samples ( n = 3 per group, ∗ p < 0.001).

    Article Snippet: For subsequent passaging, the medium was removed and the dishes were rinsed twice with phosphate-buffered saline (Nacalai Tesque).

    Techniques: Derivative Assay, Concentration Assay, Isolation, Zeta Potential Analyzer, Saline, Sandwich ELISA, Purification