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phosphate buffered saline solution  (Thermo Fisher)


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    Structured Review

    Thermo Fisher phosphate buffered saline solution
    Phosphate Buffered Saline Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphate+buffer+solution+dpbs/Phosphate-buffered+saline+(DPBS%2C+10X)%2C+Dulbecco's+formula%2C+without+calcium%2C+without+magnesium/pmc13249459-145-14-19
    Average 96 stars, based on 1 article reviews
    phosphate buffered saline solution - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Saline:

    Article Title: Endothelial soluble APP/APLP2 promote heart repair through KIT-mediated angiogenesis
    Article Snippet: HisTrap TALON columns (Cytiva, 28-9537-66, USA) were used for affinity purification, with elution in 3 ml of buffer [50 mM NaPO 4 , 300 mM NaCl, and 150 mM imidazole (pH 7.4)]. .. Eluates were desalted using PD-10 columns (Cytiva, USA), eluted in Dulbecco’s phosphate-buffered saline (PBS; Life Technologies, USA), and concentrated with a VivaspinR20 (30-kDa molecular weight cutoff) centrifugal concentrator (Sigma-Aldrich, USA), following the manufacturer’s instructions. .. Protein concentration was measured using the Bicinchoninic Acid Assay (Sigma-Aldrich, USA).

    Article Title: Intestinal resident effector-memory CD4 T cells on the adaptive-innate spectrum comprise IL-18 reactivity and adaptive CMV specificity.
    Article Snippet: .. Tissues were rinsed with phosphate- buffered saline (PBS) and processed in parallel in sterile petri dishes containing RPMI 1640 cell culture media (Gibco) supplemented with heat- inactivated fetal calf serum (10% FCS, MilliporeSigma), l- glutamine (1 mM, Invitrogen), penicillin (100 U/ml), and streptomycin (50 μg/ml) containing deoxyribonuclease I (DNase I, 1 nM, Sigma- Aldrich) (R10). ..

    Article Title: Ether lipid remodeling during neuronal differentiation prevents ferroptosis
    Article Snippet: .. Cells were washed with Dulbecco’s Phosphate-Buffered Saline (DPBS without calcium/magnesium, Gibco) two times and detached with trypsin-EDTA (Gibco), which was stopped by the addition of complete medium. .. Cells were counted using Countess II automated Cell Counter (Invitrogen) following manufacturer instructions.

    Article Title: Nitazoxanide cooperates with cytarabine to inhibit cytarabine-resistant acute myeloid leukemia progression via mitochondrial dysfunction and PLK1 suppression.
    Article Snippet: Acute myeloid leukemia (AML) remains a therapeutic challenge due to frequent resistance to cytarabine (Ara-C), the standard chemotherapeutic agent.. Recent studies implicate mitochondrial oxidative phosphorylation (OXPHOS) as a pivotal driver of this resistance, conferring metabolic adaptability and survival advantages to leukemic cells.. In this study, we evaluated the therapeutic potential of repurposing nitazoxanide (NTZ), an FDAapproved antiparasitic drug, to to overcome Ara-C resistance in AML.

    Article Title: Gpnmb defines a phagocytic state of microglia linked to cell death in prion disease mouse model.
    Article Snippet: .. The following reagent and materials were employed: Sodium phosphotungstic acid (NaPTA) (Sigma-Aldrich), OptiPrep density gradinet medium (STEMCELL Technologies), Protease type XIV from Streptomyces griseus (Sigma-Aldrich), benzonase nuclease (Sigma-Aldrich), EDTA 0.5 M, pH 8.0, RNA-free (Invitrogen), Sodium lauroylsarcosine (Sigma-Aldrich), Dulbecco's phosphate-buffered saline (D-PBS) lacking Mg2+ and Ca2+ (Gibco), Ultrafree-MC microcentrifuge filtration unit (Millipore, UFC30HV00). ..

    Article Title: A Potent CRISPR-Cas12l Double-Strand Break Gene Editor.
    Article Snippet: .. On the day of the experiment, cells were washed using Dulbecco’s phosphate saline buffer (DPBS) (Thermo Fisher Scientific) and trypsinized using TrypLE Express Enzyme (1X) (Thermo Fisher Scientific). .. On the day of the experiment, cells were washed using Dulbecco’s phosphate saline buffer (DPBS) (Thermo Fisher Scientific) and trypsinized using TrypLE Express Enzyme (1X) (Thermo Fisher Scientific).

    Article Title: Protocol for differentiation and efficient AAV-mediated gene delivery to hiPSC-derived microglia for functional studies
    Article Snippet: DMEM/F12 GlutaMax x1 , Thermo Fisher Scientific , 31331–028. .. Dulbecco’s phosphate buffered saline without Ca 2+ and Mg 2+ , DPBS (−/−) , Thermo Fisher Scientific , 14190–086. .. GlutaMax , Thermo Fisher Scientific , 35050038.

    Molecular Weight:

    Article Title: Endothelial soluble APP/APLP2 promote heart repair through KIT-mediated angiogenesis
    Article Snippet: HisTrap TALON columns (Cytiva, 28-9537-66, USA) were used for affinity purification, with elution in 3 ml of buffer [50 mM NaPO 4 , 300 mM NaCl, and 150 mM imidazole (pH 7.4)]. .. Eluates were desalted using PD-10 columns (Cytiva, USA), eluted in Dulbecco’s phosphate-buffered saline (PBS; Life Technologies, USA), and concentrated with a VivaspinR20 (30-kDa molecular weight cutoff) centrifugal concentrator (Sigma-Aldrich, USA), following the manufacturer’s instructions. .. Protein concentration was measured using the Bicinchoninic Acid Assay (Sigma-Aldrich, USA).

    Sterility:

    Article Title: Intestinal resident effector-memory CD4 T cells on the adaptive-innate spectrum comprise IL-18 reactivity and adaptive CMV specificity.
    Article Snippet: .. Tissues were rinsed with phosphate- buffered saline (PBS) and processed in parallel in sterile petri dishes containing RPMI 1640 cell culture media (Gibco) supplemented with heat- inactivated fetal calf serum (10% FCS, MilliporeSigma), l- glutamine (1 mM, Invitrogen), penicillin (100 U/ml), and streptomycin (50 μg/ml) containing deoxyribonuclease I (DNase I, 1 nM, Sigma- Aldrich) (R10). ..

    Cell Culture:

    Article Title: Intestinal resident effector-memory CD4 T cells on the adaptive-innate spectrum comprise IL-18 reactivity and adaptive CMV specificity.
    Article Snippet: .. Tissues were rinsed with phosphate- buffered saline (PBS) and processed in parallel in sterile petri dishes containing RPMI 1640 cell culture media (Gibco) supplemented with heat- inactivated fetal calf serum (10% FCS, MilliporeSigma), l- glutamine (1 mM, Invitrogen), penicillin (100 U/ml), and streptomycin (50 μg/ml) containing deoxyribonuclease I (DNase I, 1 nM, Sigma- Aldrich) (R10). ..

    Incubation:

    Article Title: Stem Cell Differentiation Disperses Transcriptional Clusters via a Conserved Surface-Condensate Trajectory.
    Article Snippet: Early neuronal differentiation was carried out as described in [ 45 ]. mESCs were plated with high density (1.5 × 10 5 cells/ cm 2 ) in serum-free ESGRO Complete Clonal Grade Medium (Millipore) to which 1,000 U/mL LIF was added. .. After 24 h, mESCs were washed 3 times with PBS (without magnesium and calcium), incubated in PBS for 3 min at room temperature, and then dissociated by incubating in 0.05% (v/v) Trypsin (Gibco) for 2 min at 37 ◦C . mESCs were plated onto 0.1% (v/v) gelatin-coated 10-cm dishes (Nunc) at 1.6 ×106 cells/dish in RHB-A (Takara– Clontech), changing media every 24 h. These differentiating cells were collected after 3, 6, 12, 24, and 48 h in RHB-A. ..

    Transcranial Magnetic Stimulation:

    Article Title: Nitazoxanide cooperates with cytarabine to inhibit cytarabine-resistant acute myeloid leukemia progression via mitochondrial dysfunction and PLK1 suppression.
    Article Snippet: Acute myeloid leukemia (AML) remains a therapeutic challenge due to frequent resistance to cytarabine (Ara-C), the standard chemotherapeutic agent.. Recent studies implicate mitochondrial oxidative phosphorylation (OXPHOS) as a pivotal driver of this resistance, conferring metabolic adaptability and survival advantages to leukemic cells.. In this study, we evaluated the therapeutic potential of repurposing nitazoxanide (NTZ), an FDAapproved antiparasitic drug, to to overcome Ara-C resistance in AML.

    Filtration:

    Article Title: Gpnmb defines a phagocytic state of microglia linked to cell death in prion disease mouse model.
    Article Snippet: .. The following reagent and materials were employed: Sodium phosphotungstic acid (NaPTA) (Sigma-Aldrich), OptiPrep density gradinet medium (STEMCELL Technologies), Protease type XIV from Streptomyces griseus (Sigma-Aldrich), benzonase nuclease (Sigma-Aldrich), EDTA 0.5 M, pH 8.0, RNA-free (Invitrogen), Sodium lauroylsarcosine (Sigma-Aldrich), Dulbecco's phosphate-buffered saline (D-PBS) lacking Mg2+ and Ca2+ (Gibco), Ultrafree-MC microcentrifuge filtration unit (Millipore, UFC30HV00). ..



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