1x pbs buffer (GE Healthcare)

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GE Healthcare 1x pbs buffer
1x Pbs Buffer, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 97/100, based on 4255 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phosphate+buffer+solution+1xpbs/PBS+Buffer/pmc07370531-4-19-5
Average 97 stars, based on 4255 article reviews

1x pbs buffer - by Bioz Stars, 2026-11

97/100 stars

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Purification:

Article Title: Directed evolution of a neutrophilic and mesophilic methanol dehydrogenase based on high-throughput and accurate measurement of formaldehyde
Article Snippet: .. Enzymes were purified using His SpinTrap columns (GE Healthcare, USA), and then dialyzed with PBS buffer. .. The protein concentration was quantified using the Pierce BCA method.

Article Title: Resolving sepsis-induced immunoparalysis via trained immunity by targeting interleukin-4 to myeloid cells
Article Snippet: .. Eluate was collected, concentrated and further purified and buffer-exchanged via size exclusion chromatography (HiLoad 16/600 Superdex 75 Increase; GE Healthcare) equilibrated with PBS storage buffer. .. The fixed cells were washed three times with ice-cold PBS, lysed at approximately 15 × 106 cells per ml in lysis buffer (20 mM HEPES pH 7.6, 1% SDS, 1× protease inhibitor cocktail (Roche)), sonicated (Bioruptor Pico, Diagenode) and centrifuged (10 min, 16,060 g, at RT).

Article Title: The UBX domain in UBXD1 organizes ubiquitin binding at the C-terminus of the VCP/p97 AAA-ATPase
Article Snippet: .. Reaction products were diluted 1:20 in 25 mM ammonium acetate (pH 4.6) and purified by strong cation exchange (SP HP 5 mL, GE Healthcare, elution with 200 mM NaCl, pH 7.5), followed by a size exclusion chromatography (HiLoad 26/60 Superdex 75 pg, GE Healthcare) in PBS buffer for final purification. .. The reaction products were analyzed by SDS-PAGE (4–20%, Mini-PROTEAN® TGXTM Precast Protein Gel, BioRad, California, USA) and scanned by using a Typhoon imager prior to Coomassie staining.

Size-exclusion Chromatography:

Pore Size:

Article Title: “Click” Reaction-Mediated Silk Fibroin-Functionalized Thiol-Branched Graphene Oxide Quantum Dots for Smart Sensing of Tetracycline
Article Snippet: .. Whatman filter paper grade 4 (pore size 20–25 μm) and PBS buffer (pH 7.4) were used for the experiments. ..

Filtration:

Article Title: HspB8 interacts with BAG3 in a "native-like" conformation forming a complex that displays chaperone-like activity.
Article Snippet: .. Before each experiment, proteins were loaded onto a Superose 12 10/300 GL gel filtration column (GE Healthcare, Life Sciences, Little Chalfont, England), pre-equilibrated with PBS buffer. ..

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