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Valiant Co Ltd component
Component, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 971 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phosphate+buffer+components/Phosphate+Buffered+Saline+(PBS)/pmc10745690-229-2-21
Average 96 stars, based on 971 article reviews
component - by Bioz Stars, 2026-10
96/100 stars

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Related Articles

Over Expression:

Article Title: Integrative Mendelian randomization and experimental validation prioritize KLF4 in the gut microbiota–pyroptosis–barrier axis of ulcerative colitis
Article Snippet: .. To achieve body-wide overexpression of KLF4, mice received an intravenous injection of AAV9-KLF4 (control: AAV9-Empty; GENCEFE Biotech, Wuxi, China) via the tail vein (~1 × 10 11 vg per 20–25 g mouse in 150–200 μL sterile phosphate-buffered saline (PBS)) 7 days before dextran sulfate sodium (DSS; MP Biomedicals, Solon, OH, USA) administration. ..

Injection:

Article Title: Integrative Mendelian randomization and experimental validation prioritize KLF4 in the gut microbiota–pyroptosis–barrier axis of ulcerative colitis
Article Snippet: .. To achieve body-wide overexpression of KLF4, mice received an intravenous injection of AAV9-KLF4 (control: AAV9-Empty; GENCEFE Biotech, Wuxi, China) via the tail vein (~1 × 10 11 vg per 20–25 g mouse in 150–200 μL sterile phosphate-buffered saline (PBS)) 7 days before dextran sulfate sodium (DSS; MP Biomedicals, Solon, OH, USA) administration. ..

Control:

Article Title: Integrative Mendelian randomization and experimental validation prioritize KLF4 in the gut microbiota–pyroptosis–barrier axis of ulcerative colitis
Article Snippet: .. To achieve body-wide overexpression of KLF4, mice received an intravenous injection of AAV9-KLF4 (control: AAV9-Empty; GENCEFE Biotech, Wuxi, China) via the tail vein (~1 × 10 11 vg per 20–25 g mouse in 150–200 μL sterile phosphate-buffered saline (PBS)) 7 days before dextran sulfate sodium (DSS; MP Biomedicals, Solon, OH, USA) administration. ..

Sterility:

Article Title: Integrative Mendelian randomization and experimental validation prioritize KLF4 in the gut microbiota–pyroptosis–barrier axis of ulcerative colitis
Article Snippet: .. To achieve body-wide overexpression of KLF4, mice received an intravenous injection of AAV9-KLF4 (control: AAV9-Empty; GENCEFE Biotech, Wuxi, China) via the tail vein (~1 × 10 11 vg per 20–25 g mouse in 150–200 μL sterile phosphate-buffered saline (PBS)) 7 days before dextran sulfate sodium (DSS; MP Biomedicals, Solon, OH, USA) administration. ..

Article Title: Genome sequence of the dimorphic Dothioraceae fungal isolate EMM_F3.
Article Snippet: .. Leaf strips, 4 cm by 10 cm long, were cut with flame-sterilized scissors into a sterile 50 mL tube and vortexed in 1× phosphate-buffered saline for 1 min. One hundred microliters of leaf wash were spread onto Petri dishes containing Difco malt extract agar medium (BD Biosciences, NJ, USA) amended with 1 g of yeast extract (MP Biomedicals, OH, USA), rifampicin (0.01 mg/mL), and chloramphenicol (0.1 mg/mL). ..

Article Title: Methods for controlling treatment volumes, thermal gradients, muscle stimulation, and immune responses in pulsed electric field treatments
Article Snippet: .. Stock solutions of 1 mg/mL propidium iodide (MP Biomedicals, Santa Ana, CA) were prepared by adding 25 mL of sterile PBS (MP Biomedicals, Santa Ana, CA) to 25 mg of powdered dye and stored at 4° C. Stock solutions of 4 μM Calcein AM (Invitrogen, Carlsbad, CA) were prepared by adding 125 μL of sterile DMSO (MP Biomedicals, Santa Ana, CA) to 50 of powdered dye and stored at −20° C. A mixture containing 2 μL Calcein AM (live stain, green), 100 μL of PI (dead stain, red) stock solutions, and 100 μL of Phosphate Buffered Saline (PBS, MP Biomedicals, Santa Ana, CA) was added to each well 24 hours after treatments. ..

Article Title: Preliminary study on the effects of sub-MIC concentrations of octenidine and polyhexanidine on biofilms produced by animal isolates of Pseudomonas aeruginosa.
Article Snippet: .. For the final analysis, samples were stained with crystal violet (0.2% aqueous solution, AQUA-MED, Łódź, Poland) The wells were rinsed three times with sterile phosphate-buffered saline (PBS) (MP Biomedicals Europe, Warszawa, Poland) to remove non-adherent cells. ..

Saline:

Article Title: Integrative Mendelian randomization and experimental validation prioritize KLF4 in the gut microbiota–pyroptosis–barrier axis of ulcerative colitis
Article Snippet: .. To achieve body-wide overexpression of KLF4, mice received an intravenous injection of AAV9-KLF4 (control: AAV9-Empty; GENCEFE Biotech, Wuxi, China) via the tail vein (~1 × 10 11 vg per 20–25 g mouse in 150–200 μL sterile phosphate-buffered saline (PBS)) 7 days before dextran sulfate sodium (DSS; MP Biomedicals, Solon, OH, USA) administration. ..

Article Title: Genome sequence of the dimorphic Dothioraceae fungal isolate EMM_F3.
Article Snippet: .. Leaf strips, 4 cm by 10 cm long, were cut with flame-sterilized scissors into a sterile 50 mL tube and vortexed in 1× phosphate-buffered saline for 1 min. One hundred microliters of leaf wash were spread onto Petri dishes containing Difco malt extract agar medium (BD Biosciences, NJ, USA) amended with 1 g of yeast extract (MP Biomedicals, OH, USA), rifampicin (0.01 mg/mL), and chloramphenicol (0.1 mg/mL). ..

Article Title: Methods for controlling treatment volumes, thermal gradients, muscle stimulation, and immune responses in pulsed electric field treatments
Article Snippet: .. Stock solutions of 1 mg/mL propidium iodide (MP Biomedicals, Santa Ana, CA) were prepared by adding 25 mL of sterile PBS (MP Biomedicals, Santa Ana, CA) to 25 mg of powdered dye and stored at 4° C. Stock solutions of 4 μM Calcein AM (Invitrogen, Carlsbad, CA) were prepared by adding 125 μL of sterile DMSO (MP Biomedicals, Santa Ana, CA) to 50 of powdered dye and stored at −20° C. A mixture containing 2 μL Calcein AM (live stain, green), 100 μL of PI (dead stain, red) stock solutions, and 100 μL of Phosphate Buffered Saline (PBS, MP Biomedicals, Santa Ana, CA) was added to each well 24 hours after treatments. ..

Article Title: Negative-Pressure Induces Epithelial-Mesenchymal Transition via Thrombospondin-1 Upregulation in Intact Diabetic Skin.
Article Snippet: .. Circular skin biopsies (1 cm in diameter) were homogenised in 1000 μL of cold phosphate- buffered saline containing 1% Triton X- 100 (MP Biomedicals, Irvine, California, USA) and 0.1% protease inhibitor cocktail (Sigma- Aldrich, Burlington, Massachusetts, USA) using a μT- 12 tissue homogeniser (TAITEC, Saitama, Japan) at 3200 rpm for 2 min. Homogenates were centrifuged at 15000 ×g for 5 min, and the resulting supernatants were collected for quantification of THBS1, TGF- β1, PAI- 1, HIF- 1α and vascular endothelial growth factor (VEGF) using specific ELISA kits (Table S1). ..

Article Title: Preliminary study on the effects of sub-MIC concentrations of octenidine and polyhexanidine on biofilms produced by animal isolates of Pseudomonas aeruginosa.
Article Snippet: .. For the final analysis, samples were stained with crystal violet (0.2% aqueous solution, AQUA-MED, Łódź, Poland) The wells were rinsed three times with sterile phosphate-buffered saline (PBS) (MP Biomedicals Europe, Warszawa, Poland) to remove non-adherent cells. ..

Article Title: Etiological and immunological aspects of equine hoof canker
Article Snippet: Subsequently, erythrocytes were lysed by adding 1 mL of BD FACS Lysing Solution (BD Biosciences, USA) and incubating for an additional 20 min under the same conditions. .. Samples were then centrifuged at 300 × g for 5 min, and the resulting cell pellets were washed twice with 1 mL phosphate-buffered saline (PBS; MP Biomedicals, France), with centrifugation at 300 × g for 5 min after each wash. ..

Article Title: Protein-saliva interactions govern the structure and lubrication of salivary films.
Article Snippet: .. Bovine serum albumin (BSA, ≥98%, Sigma-Aldrich, Co., USA) is dissolved at 50 ◦C in 70 mM phosphate buffered saline (PBS, pH 7.4, without calcium and magnesium, MP Biomedicals, LLC,USA) at four concentrations: 0.6 mg/ml (the concentration of BSA in skim milk [43]), 1.3 mg/ml (the average protein level in saliva [44]), 5.2 mg/ml and 10.4 mg/ml. ..

Article Title: Membranes arrest the coarsening of mitochondrial condensates in human cells.
Article Snippet: For imaging, slides were cured with Prolong Gold (Invitrogen P36930) at least 24 hours prior to imaging. .. Cells were fixed in 4% paraformaldehyde (PFA) (Electron microscopy sciences, 15710) by diluting 16% PFA 1:1 in phosphate-buffered saline (PBS) (MP-Biomedicals,1860454) (8%) and 1:1 (final 4% PFA) directly in cell culture media for 10 min. Then cells were washed with PBS before permeabilization with 0.5% Triton X-100 (Sigma-aldrich, T8787) in PBS (v/v) for 10 min at room temperature (RT). .. After the activation, cells were immediately fixed in 4% PFA (Electron Microscopy Science Cat.15710) for 10 min at room temperature.

Staining:

Article Title: Methods for controlling treatment volumes, thermal gradients, muscle stimulation, and immune responses in pulsed electric field treatments
Article Snippet: .. Stock solutions of 1 mg/mL propidium iodide (MP Biomedicals, Santa Ana, CA) were prepared by adding 25 mL of sterile PBS (MP Biomedicals, Santa Ana, CA) to 25 mg of powdered dye and stored at 4° C. Stock solutions of 4 μM Calcein AM (Invitrogen, Carlsbad, CA) were prepared by adding 125 μL of sterile DMSO (MP Biomedicals, Santa Ana, CA) to 50 of powdered dye and stored at −20° C. A mixture containing 2 μL Calcein AM (live stain, green), 100 μL of PI (dead stain, red) stock solutions, and 100 μL of Phosphate Buffered Saline (PBS, MP Biomedicals, Santa Ana, CA) was added to each well 24 hours after treatments. ..

Article Title: Preliminary study on the effects of sub-MIC concentrations of octenidine and polyhexanidine on biofilms produced by animal isolates of Pseudomonas aeruginosa.
Article Snippet: .. For the final analysis, samples were stained with crystal violet (0.2% aqueous solution, AQUA-MED, Łódź, Poland) The wells were rinsed three times with sterile phosphate-buffered saline (PBS) (MP Biomedicals Europe, Warszawa, Poland) to remove non-adherent cells. ..

Protease Inhibitor:

Article Title: Negative-Pressure Induces Epithelial-Mesenchymal Transition via Thrombospondin-1 Upregulation in Intact Diabetic Skin.
Article Snippet: .. Circular skin biopsies (1 cm in diameter) were homogenised in 1000 μL of cold phosphate- buffered saline containing 1% Triton X- 100 (MP Biomedicals, Irvine, California, USA) and 0.1% protease inhibitor cocktail (Sigma- Aldrich, Burlington, Massachusetts, USA) using a μT- 12 tissue homogeniser (TAITEC, Saitama, Japan) at 3200 rpm for 2 min. Homogenates were centrifuged at 15000 ×g for 5 min, and the resulting supernatants were collected for quantification of THBS1, TGF- β1, PAI- 1, HIF- 1α and vascular endothelial growth factor (VEGF) using specific ELISA kits (Table S1). ..

Enzyme-linked Immunosorbent Assay:

Article Title: Negative-Pressure Induces Epithelial-Mesenchymal Transition via Thrombospondin-1 Upregulation in Intact Diabetic Skin.
Article Snippet: .. Circular skin biopsies (1 cm in diameter) were homogenised in 1000 μL of cold phosphate- buffered saline containing 1% Triton X- 100 (MP Biomedicals, Irvine, California, USA) and 0.1% protease inhibitor cocktail (Sigma- Aldrich, Burlington, Massachusetts, USA) using a μT- 12 tissue homogeniser (TAITEC, Saitama, Japan) at 3200 rpm for 2 min. Homogenates were centrifuged at 15000 ×g for 5 min, and the resulting supernatants were collected for quantification of THBS1, TGF- β1, PAI- 1, HIF- 1α and vascular endothelial growth factor (VEGF) using specific ELISA kits (Table S1). ..

Centrifugation:

Article Title: Etiological and immunological aspects of equine hoof canker
Article Snippet: Subsequently, erythrocytes were lysed by adding 1 mL of BD FACS Lysing Solution (BD Biosciences, USA) and incubating for an additional 20 min under the same conditions. .. Samples were then centrifuged at 300 × g for 5 min, and the resulting cell pellets were washed twice with 1 mL phosphate-buffered saline (PBS; MP Biomedicals, France), with centrifugation at 300 × g for 5 min after each wash. ..

Concentration Assay:

Article Title: Protein-saliva interactions govern the structure and lubrication of salivary films.
Article Snippet: .. Bovine serum albumin (BSA, ≥98%, Sigma-Aldrich, Co., USA) is dissolved at 50 ◦C in 70 mM phosphate buffered saline (PBS, pH 7.4, without calcium and magnesium, MP Biomedicals, LLC,USA) at four concentrations: 0.6 mg/ml (the concentration of BSA in skim milk [43]), 1.3 mg/ml (the average protein level in saliva [44]), 5.2 mg/ml and 10.4 mg/ml. ..

Electron Microscopy:

Article Title: Membranes arrest the coarsening of mitochondrial condensates in human cells.
Article Snippet: For imaging, slides were cured with Prolong Gold (Invitrogen P36930) at least 24 hours prior to imaging. .. Cells were fixed in 4% paraformaldehyde (PFA) (Electron microscopy sciences, 15710) by diluting 16% PFA 1:1 in phosphate-buffered saline (PBS) (MP-Biomedicals,1860454) (8%) and 1:1 (final 4% PFA) directly in cell culture media for 10 min. Then cells were washed with PBS before permeabilization with 0.5% Triton X-100 (Sigma-aldrich, T8787) in PBS (v/v) for 10 min at room temperature (RT). .. After the activation, cells were immediately fixed in 4% PFA (Electron Microscopy Science Cat.15710) for 10 min at room temperature.

Cell Culture:

Article Title: Membranes arrest the coarsening of mitochondrial condensates in human cells.
Article Snippet: For imaging, slides were cured with Prolong Gold (Invitrogen P36930) at least 24 hours prior to imaging. .. Cells were fixed in 4% paraformaldehyde (PFA) (Electron microscopy sciences, 15710) by diluting 16% PFA 1:1 in phosphate-buffered saline (PBS) (MP-Biomedicals,1860454) (8%) and 1:1 (final 4% PFA) directly in cell culture media for 10 min. Then cells were washed with PBS before permeabilization with 0.5% Triton X-100 (Sigma-aldrich, T8787) in PBS (v/v) for 10 min at room temperature (RT). .. After the activation, cells were immediately fixed in 4% PFA (Electron Microscopy Science Cat.15710) for 10 min at room temperature.



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