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phenylephrine hydrochloride ophthalmic solution  (Valeant Pharmaceuticals)

 
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    Valeant Pharmaceuticals phenylephrine hydrochloride ophthalmic solution
    Phenylephrine Hydrochloride Ophthalmic Solution, supplied by Valeant Pharmaceuticals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phenylephrine/ophthalmic+solution/pm41800844-72-14-20
    Average 86 stars, based on 1 article reviews
    phenylephrine hydrochloride ophthalmic solution - by Bioz Stars, 2026-10
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    Article Title: Photodynamic therapy with infracyanine green induces keratocyte depopulation in the normal equine cornea.
    Article Snippet: Funding information ACVO (American College of Veterinary Ophthalmologists) Vision for Animals Foundation, Grant/Award Number: VAF2024-5 Abstract Background: Use of photodynamic therapy (PDT) for the treatment of immunemediated keratitis (IMMK) in horses is becoming common.. The safety and morphologic impact on the normal equine cornea have not been investigated, and the mechanism of its efficacy is unknown.. Objective: To investigate the morphologic effects and safety of in vivo PDT on healthy equine corneas.

    Eye Drops:

    Article Title: Enhancing Experience-Dependent Plasticity Accelerates Vision Loss in a Murine Model of Retinitis Pigmentosa.
    Article Snippet: .. Pupils were dilated and accommodation relaxed with the application of eye drops, with 2.5% phenylephrine hydrochloride ophthalmic solution (NDC 82260-102-10; Bausch + Lomb, Bridgewater, NJ, USA) and 0.5% tropicamide ophthalmic solution (NDC 24208-590-64; Bausch + Lomb) for 30 seconds. ..

    Article Title: Enhancing Experience-Dependent Plasticity Accelerates Vision Loss in a Murine Model of Retinitis Pigmentosa
    Article Snippet: .. Pupils were dilated and accommodation relaxed with the application of eye drops, with 2.5% phenylephrine hydrochloride ophthalmic solution (NDC 82260-102-10; Bausch + Lomb, Bridgewater, NJ, USA) and 0.5% tropicamide ophthalmic solution (NDC 24208-590-64; Bausch + Lomb) for 30 seconds. ..

    Article Title: A mouse model for cerebral/cortical visual impairment (CVI) impairs vision and disrupts the spatial frequency tuning of neurons in visual cortex.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.



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    2SM promotes cardiomyocyte proliferation in vitro. A Schematic illustrating the screening strategy for simplified dual-compound combinations derived from the original five-compound cocktail (5SM). The 5SM formulation includes <t>phenylephrine</t> <t>hydrochloride</t> (PE), baricitinib (BA), harmine (HM), VO-OHpic trihydrate (VO), and AZD3965 (AZ). Excluding AZ, the remaining four compounds were tested in six pairwise combinations. Among them, the PE + HM combination (termed 2SM) was selected for its robust proliferative effect. B Representative images of FUCCI-positive P3 NRVMs. NRVMs were infected with Adeno- Tnnt2 -FUCCI adenovirus and then treated with DMSO (control), single compounds or indicated compound combinations for 48 h. Scale bar, 100 μm. C Quantification of FUCCI-positive cardiomyocytes (CMs) from ( B ). n = 3 per group. Data represent mean ± SEM, with individual data points shown as dots overlaid on the bar graphs. Statistical analysis by one-way ANOVA (ns, not significant; ** P < 0.01, *** P < 0.001, **** P < 0.0001). D Representative images and quantification of Aurora kinase B (AURKB)-positive midbodies in NRVMs after treatment with DMSO, single compounds or indicated compound combinations for 48 h. NRVMs were identified by TNNT2 (cardiac troponin T) staining (red). Scale bar, 25 μm. n = 3 per group. Data represent mean ± SEM, with individual data points shown as dots overlaid on the bar graphs. Statistical analysis by one-way ANOVA (**** P < 0.0001). E – G Representative images and quantification of KI67-positive ( E ), pH3-positive ( F ) and BrdU-positive ( G ) NRVMs after treatment with DMSO, PE, HM or 2SM for 48 h. Scale bar, 50 μm. n = 3 per group. Data represent mean ± SEM, with individual data points shown as dots overlaid on the bar graphs. Statistical analysis by one-way ANOVA (ns, not significant; * P < 0.05, *** P < 0.001, **** P < 0.0001). H Representative images and quantification of single-colored MADM mouse cardiomyocytes. MADM cardiomyocytes were infected with Adeno- Tnnt2 -Cre adenovirus and then treated with DMSO, PE, HM or 2SM for 72 h. Scale bar, 100 μm. n = 3 per group. Data represent mean ± SEM, with individual data points shown as dots overlaid on the bar graphs. Statistical analysis by one-way ANOVA (ns, not significant; **** P < 0.0001)
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    hy-b0769  (MedChemExpress)
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    2SM promotes cardiomyocyte proliferation in vitro. A Schematic illustrating the screening strategy for simplified dual-compound combinations derived from the original five-compound cocktail (5SM). The 5SM formulation includes <t>phenylephrine</t> <t>hydrochloride</t> (PE), baricitinib (BA), harmine (HM), VO-OHpic trihydrate (VO), and AZD3965 (AZ). Excluding AZ, the remaining four compounds were tested in six pairwise combinations. Among them, the PE + HM combination (termed 2SM) was selected for its robust proliferative effect. B Representative images of FUCCI-positive P3 NRVMs. NRVMs were infected with Adeno- Tnnt2 -FUCCI adenovirus and then treated with DMSO (control), single compounds or indicated compound combinations for 48 h. Scale bar, 100 μm. C Quantification of FUCCI-positive cardiomyocytes (CMs) from ( B ). n = 3 per group. Data represent mean ± SEM, with individual data points shown as dots overlaid on the bar graphs. Statistical analysis by one-way ANOVA (ns, not significant; ** P < 0.01, *** P < 0.001, **** P < 0.0001). D Representative images and quantification of Aurora kinase B (AURKB)-positive midbodies in NRVMs after treatment with DMSO, single compounds or indicated compound combinations for 48 h. NRVMs were identified by TNNT2 (cardiac troponin T) staining (red). Scale bar, 25 μm. n = 3 per group. Data represent mean ± SEM, with individual data points shown as dots overlaid on the bar graphs. Statistical analysis by one-way ANOVA (**** P < 0.0001). E – G Representative images and quantification of KI67-positive ( E ), pH3-positive ( F ) and BrdU-positive ( G ) NRVMs after treatment with DMSO, PE, HM or 2SM for 48 h. Scale bar, 50 μm. n = 3 per group. Data represent mean ± SEM, with individual data points shown as dots overlaid on the bar graphs. Statistical analysis by one-way ANOVA (ns, not significant; * P < 0.05, *** P < 0.001, **** P < 0.0001). H Representative images and quantification of single-colored MADM mouse cardiomyocytes. MADM cardiomyocytes were infected with Adeno- Tnnt2 -Cre adenovirus and then treated with DMSO, PE, HM or 2SM for 72 h. Scale bar, 100 μm. n = 3 per group. Data represent mean ± SEM, with individual data points shown as dots overlaid on the bar graphs. Statistical analysis by one-way ANOVA (ns, not significant; **** P < 0.0001)
    Hy B0769, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    phenylephrine hydrochloride  (Akorn Inc)
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    2SM promotes cardiomyocyte proliferation in vitro. A Schematic illustrating the screening strategy for simplified dual-compound combinations derived from the original five-compound cocktail (5SM). The 5SM formulation includes <t>phenylephrine</t> <t>hydrochloride</t> (PE), baricitinib (BA), harmine (HM), VO-OHpic trihydrate (VO), and AZD3965 (AZ). Excluding AZ, the remaining four compounds were tested in six pairwise combinations. Among them, the PE + HM combination (termed 2SM) was selected for its robust proliferative effect. B Representative images of FUCCI-positive P3 NRVMs. NRVMs were infected with Adeno- Tnnt2 -FUCCI adenovirus and then treated with DMSO (control), single compounds or indicated compound combinations for 48 h. Scale bar, 100 μm. C Quantification of FUCCI-positive cardiomyocytes (CMs) from ( B ). n = 3 per group. Data represent mean ± SEM, with individual data points shown as dots overlaid on the bar graphs. Statistical analysis by one-way ANOVA (ns, not significant; ** P < 0.01, *** P < 0.001, **** P < 0.0001). D Representative images and quantification of Aurora kinase B (AURKB)-positive midbodies in NRVMs after treatment with DMSO, single compounds or indicated compound combinations for 48 h. NRVMs were identified by TNNT2 (cardiac troponin T) staining (red). Scale bar, 25 μm. n = 3 per group. Data represent mean ± SEM, with individual data points shown as dots overlaid on the bar graphs. Statistical analysis by one-way ANOVA (**** P < 0.0001). E – G Representative images and quantification of KI67-positive ( E ), pH3-positive ( F ) and BrdU-positive ( G ) NRVMs after treatment with DMSO, PE, HM or 2SM for 48 h. Scale bar, 50 μm. n = 3 per group. Data represent mean ± SEM, with individual data points shown as dots overlaid on the bar graphs. Statistical analysis by one-way ANOVA (ns, not significant; * P < 0.05, *** P < 0.001, **** P < 0.0001). H Representative images and quantification of single-colored MADM mouse cardiomyocytes. MADM cardiomyocytes were infected with Adeno- Tnnt2 -Cre adenovirus and then treated with DMSO, PE, HM or 2SM for 72 h. Scale bar, 100 μm. n = 3 per group. Data represent mean ± SEM, with individual data points shown as dots overlaid on the bar graphs. Statistical analysis by one-way ANOVA (ns, not significant; **** P < 0.0001)
    Phenylephrine Hydrochloride, supplied by Akorn Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    pe59  (MedChemExpress)
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    2SM promotes cardiomyocyte proliferation in vitro. A Schematic illustrating the screening strategy for simplified dual-compound combinations derived from the original five-compound cocktail (5SM). The 5SM formulation includes <t>phenylephrine</t> <t>hydrochloride</t> (PE), baricitinib (BA), harmine (HM), VO-OHpic trihydrate (VO), and AZD3965 (AZ). Excluding AZ, the remaining four compounds were tested in six pairwise combinations. Among them, the PE + HM combination (termed 2SM) was selected for its robust proliferative effect. B Representative images of FUCCI-positive P3 NRVMs. NRVMs were infected with Adeno- Tnnt2 -FUCCI adenovirus and then treated with DMSO (control), single compounds or indicated compound combinations for 48 h. Scale bar, 100 μm. C Quantification of FUCCI-positive cardiomyocytes (CMs) from ( B ). n = 3 per group. Data represent mean ± SEM, with individual data points shown as dots overlaid on the bar graphs. Statistical analysis by one-way ANOVA (ns, not significant; ** P < 0.01, *** P < 0.001, **** P < 0.0001). D Representative images and quantification of Aurora kinase B (AURKB)-positive midbodies in NRVMs after treatment with DMSO, single compounds or indicated compound combinations for 48 h. NRVMs were identified by TNNT2 (cardiac troponin T) staining (red). Scale bar, 25 μm. n = 3 per group. Data represent mean ± SEM, with individual data points shown as dots overlaid on the bar graphs. Statistical analysis by one-way ANOVA (**** P < 0.0001). E – G Representative images and quantification of KI67-positive ( E ), pH3-positive ( F ) and BrdU-positive ( G ) NRVMs after treatment with DMSO, PE, HM or 2SM for 48 h. Scale bar, 50 μm. n = 3 per group. Data represent mean ± SEM, with individual data points shown as dots overlaid on the bar graphs. Statistical analysis by one-way ANOVA (ns, not significant; * P < 0.05, *** P < 0.001, **** P < 0.0001). H Representative images and quantification of single-colored MADM mouse cardiomyocytes. MADM cardiomyocytes were infected with Adeno- Tnnt2 -Cre adenovirus and then treated with DMSO, PE, HM or 2SM for 72 h. Scale bar, 100 μm. n = 3 per group. Data represent mean ± SEM, with individual data points shown as dots overlaid on the bar graphs. Statistical analysis by one-way ANOVA (ns, not significant; **** P < 0.0001)
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    concentration response curves to phenylephrine  (MedChemExpress)
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    2SM promotes cardiomyocyte proliferation in vitro. A Schematic illustrating the screening strategy for simplified dual-compound combinations derived from the original five-compound cocktail (5SM). The 5SM formulation includes <t>phenylephrine</t> <t>hydrochloride</t> (PE), baricitinib (BA), harmine (HM), VO-OHpic trihydrate (VO), and AZD3965 (AZ). Excluding AZ, the remaining four compounds were tested in six pairwise combinations. Among them, the PE + HM combination (termed 2SM) was selected for its robust proliferative effect. B Representative images of FUCCI-positive P3 NRVMs. NRVMs were infected with Adeno- Tnnt2 -FUCCI adenovirus and then treated with DMSO (control), single compounds or indicated compound combinations for 48 h. Scale bar, 100 μm. C Quantification of FUCCI-positive cardiomyocytes (CMs) from ( B ). n = 3 per group. Data represent mean ± SEM, with individual data points shown as dots overlaid on the bar graphs. Statistical analysis by one-way ANOVA (ns, not significant; ** P < 0.01, *** P < 0.001, **** P < 0.0001). D Representative images and quantification of Aurora kinase B (AURKB)-positive midbodies in NRVMs after treatment with DMSO, single compounds or indicated compound combinations for 48 h. NRVMs were identified by TNNT2 (cardiac troponin T) staining (red). Scale bar, 25 μm. n = 3 per group. Data represent mean ± SEM, with individual data points shown as dots overlaid on the bar graphs. Statistical analysis by one-way ANOVA (**** P < 0.0001). E – G Representative images and quantification of KI67-positive ( E ), pH3-positive ( F ) and BrdU-positive ( G ) NRVMs after treatment with DMSO, PE, HM or 2SM for 48 h. Scale bar, 50 μm. n = 3 per group. Data represent mean ± SEM, with individual data points shown as dots overlaid on the bar graphs. Statistical analysis by one-way ANOVA (ns, not significant; * P < 0.05, *** P < 0.001, **** P < 0.0001). H Representative images and quantification of single-colored MADM mouse cardiomyocytes. MADM cardiomyocytes were infected with Adeno- Tnnt2 -Cre adenovirus and then treated with DMSO, PE, HM or 2SM for 72 h. Scale bar, 100 μm. n = 3 per group. Data represent mean ± SEM, with individual data points shown as dots overlaid on the bar graphs. Statistical analysis by one-way ANOVA (ns, not significant; **** P < 0.0001)
    Concentration Response Curves To Phenylephrine, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    phenylephrine  (Valeant Pharmaceuticals)
    86
    Valeant Pharmaceuticals phenylephrine
    2SM promotes cardiomyocyte proliferation in vitro. A Schematic illustrating the screening strategy for simplified dual-compound combinations derived from the original five-compound cocktail (5SM). The 5SM formulation includes <t>phenylephrine</t> <t>hydrochloride</t> (PE), baricitinib (BA), harmine (HM), VO-OHpic trihydrate (VO), and AZD3965 (AZ). Excluding AZ, the remaining four compounds were tested in six pairwise combinations. Among them, the PE + HM combination (termed 2SM) was selected for its robust proliferative effect. B Representative images of FUCCI-positive P3 NRVMs. NRVMs were infected with Adeno- Tnnt2 -FUCCI adenovirus and then treated with DMSO (control), single compounds or indicated compound combinations for 48 h. Scale bar, 100 μm. C Quantification of FUCCI-positive cardiomyocytes (CMs) from ( B ). n = 3 per group. Data represent mean ± SEM, with individual data points shown as dots overlaid on the bar graphs. Statistical analysis by one-way ANOVA (ns, not significant; ** P < 0.01, *** P < 0.001, **** P < 0.0001). D Representative images and quantification of Aurora kinase B (AURKB)-positive midbodies in NRVMs after treatment with DMSO, single compounds or indicated compound combinations for 48 h. NRVMs were identified by TNNT2 (cardiac troponin T) staining (red). Scale bar, 25 μm. n = 3 per group. Data represent mean ± SEM, with individual data points shown as dots overlaid on the bar graphs. Statistical analysis by one-way ANOVA (**** P < 0.0001). E – G Representative images and quantification of KI67-positive ( E ), pH3-positive ( F ) and BrdU-positive ( G ) NRVMs after treatment with DMSO, PE, HM or 2SM for 48 h. Scale bar, 50 μm. n = 3 per group. Data represent mean ± SEM, with individual data points shown as dots overlaid on the bar graphs. Statistical analysis by one-way ANOVA (ns, not significant; * P < 0.05, *** P < 0.001, **** P < 0.0001). H Representative images and quantification of single-colored MADM mouse cardiomyocytes. MADM cardiomyocytes were infected with Adeno- Tnnt2 -Cre adenovirus and then treated with DMSO, PE, HM or 2SM for 72 h. Scale bar, 100 μm. n = 3 per group. Data represent mean ± SEM, with individual data points shown as dots overlaid on the bar graphs. Statistical analysis by one-way ANOVA (ns, not significant; **** P < 0.0001)
    Phenylephrine, supplied by Valeant Pharmaceuticals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    phenylephrine hydrochloride ophthalmic solution  (Akorn Inc)
    86
    Akorn Inc phenylephrine hydrochloride ophthalmic solution
    2SM promotes cardiomyocyte proliferation in vitro. A Schematic illustrating the screening strategy for simplified dual-compound combinations derived from the original five-compound cocktail (5SM). The 5SM formulation includes <t>phenylephrine</t> <t>hydrochloride</t> (PE), baricitinib (BA), harmine (HM), VO-OHpic trihydrate (VO), and AZD3965 (AZ). Excluding AZ, the remaining four compounds were tested in six pairwise combinations. Among them, the PE + HM combination (termed 2SM) was selected for its robust proliferative effect. B Representative images of FUCCI-positive P3 NRVMs. NRVMs were infected with Adeno- Tnnt2 -FUCCI adenovirus and then treated with DMSO (control), single compounds or indicated compound combinations for 48 h. Scale bar, 100 μm. C Quantification of FUCCI-positive cardiomyocytes (CMs) from ( B ). n = 3 per group. Data represent mean ± SEM, with individual data points shown as dots overlaid on the bar graphs. Statistical analysis by one-way ANOVA (ns, not significant; ** P < 0.01, *** P < 0.001, **** P < 0.0001). D Representative images and quantification of Aurora kinase B (AURKB)-positive midbodies in NRVMs after treatment with DMSO, single compounds or indicated compound combinations for 48 h. NRVMs were identified by TNNT2 (cardiac troponin T) staining (red). Scale bar, 25 μm. n = 3 per group. Data represent mean ± SEM, with individual data points shown as dots overlaid on the bar graphs. Statistical analysis by one-way ANOVA (**** P < 0.0001). E – G Representative images and quantification of KI67-positive ( E ), pH3-positive ( F ) and BrdU-positive ( G ) NRVMs after treatment with DMSO, PE, HM or 2SM for 48 h. Scale bar, 50 μm. n = 3 per group. Data represent mean ± SEM, with individual data points shown as dots overlaid on the bar graphs. Statistical analysis by one-way ANOVA (ns, not significant; * P < 0.05, *** P < 0.001, **** P < 0.0001). H Representative images and quantification of single-colored MADM mouse cardiomyocytes. MADM cardiomyocytes were infected with Adeno- Tnnt2 -Cre adenovirus and then treated with DMSO, PE, HM or 2SM for 72 h. Scale bar, 100 μm. n = 3 per group. Data represent mean ± SEM, with individual data points shown as dots overlaid on the bar graphs. Statistical analysis by one-way ANOVA (ns, not significant; **** P < 0.0001)
    Phenylephrine Hydrochloride Ophthalmic Solution, supplied by Akorn Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    2SM promotes cardiomyocyte proliferation in vitro. A Schematic illustrating the screening strategy for simplified dual-compound combinations derived from the original five-compound cocktail (5SM). The 5SM formulation includes phenylephrine hydrochloride (PE), baricitinib (BA), harmine (HM), VO-OHpic trihydrate (VO), and AZD3965 (AZ). Excluding AZ, the remaining four compounds were tested in six pairwise combinations. Among them, the PE + HM combination (termed 2SM) was selected for its robust proliferative effect. B Representative images of FUCCI-positive P3 NRVMs. NRVMs were infected with Adeno- Tnnt2 -FUCCI adenovirus and then treated with DMSO (control), single compounds or indicated compound combinations for 48 h. Scale bar, 100 μm. C Quantification of FUCCI-positive cardiomyocytes (CMs) from ( B ). n = 3 per group. Data represent mean ± SEM, with individual data points shown as dots overlaid on the bar graphs. Statistical analysis by one-way ANOVA (ns, not significant; ** P < 0.01, *** P < 0.001, **** P < 0.0001). D Representative images and quantification of Aurora kinase B (AURKB)-positive midbodies in NRVMs after treatment with DMSO, single compounds or indicated compound combinations for 48 h. NRVMs were identified by TNNT2 (cardiac troponin T) staining (red). Scale bar, 25 μm. n = 3 per group. Data represent mean ± SEM, with individual data points shown as dots overlaid on the bar graphs. Statistical analysis by one-way ANOVA (**** P < 0.0001). E – G Representative images and quantification of KI67-positive ( E ), pH3-positive ( F ) and BrdU-positive ( G ) NRVMs after treatment with DMSO, PE, HM or 2SM for 48 h. Scale bar, 50 μm. n = 3 per group. Data represent mean ± SEM, with individual data points shown as dots overlaid on the bar graphs. Statistical analysis by one-way ANOVA (ns, not significant; * P < 0.05, *** P < 0.001, **** P < 0.0001). H Representative images and quantification of single-colored MADM mouse cardiomyocytes. MADM cardiomyocytes were infected with Adeno- Tnnt2 -Cre adenovirus and then treated with DMSO, PE, HM or 2SM for 72 h. Scale bar, 100 μm. n = 3 per group. Data represent mean ± SEM, with individual data points shown as dots overlaid on the bar graphs. Statistical analysis by one-way ANOVA (ns, not significant; **** P < 0.0001)

    Journal: Cell Regeneration

    Article Title: Small-molecule cocktail 2SM promotes heart regeneration via inducing embryonic-like cardiomyocyte state

    doi: 10.1186/s13619-026-00301-0

    Figure Lengend Snippet: 2SM promotes cardiomyocyte proliferation in vitro. A Schematic illustrating the screening strategy for simplified dual-compound combinations derived from the original five-compound cocktail (5SM). The 5SM formulation includes phenylephrine hydrochloride (PE), baricitinib (BA), harmine (HM), VO-OHpic trihydrate (VO), and AZD3965 (AZ). Excluding AZ, the remaining four compounds were tested in six pairwise combinations. Among them, the PE + HM combination (termed 2SM) was selected for its robust proliferative effect. B Representative images of FUCCI-positive P3 NRVMs. NRVMs were infected with Adeno- Tnnt2 -FUCCI adenovirus and then treated with DMSO (control), single compounds or indicated compound combinations for 48 h. Scale bar, 100 μm. C Quantification of FUCCI-positive cardiomyocytes (CMs) from ( B ). n = 3 per group. Data represent mean ± SEM, with individual data points shown as dots overlaid on the bar graphs. Statistical analysis by one-way ANOVA (ns, not significant; ** P < 0.01, *** P < 0.001, **** P < 0.0001). D Representative images and quantification of Aurora kinase B (AURKB)-positive midbodies in NRVMs after treatment with DMSO, single compounds or indicated compound combinations for 48 h. NRVMs were identified by TNNT2 (cardiac troponin T) staining (red). Scale bar, 25 μm. n = 3 per group. Data represent mean ± SEM, with individual data points shown as dots overlaid on the bar graphs. Statistical analysis by one-way ANOVA (**** P < 0.0001). E – G Representative images and quantification of KI67-positive ( E ), pH3-positive ( F ) and BrdU-positive ( G ) NRVMs after treatment with DMSO, PE, HM or 2SM for 48 h. Scale bar, 50 μm. n = 3 per group. Data represent mean ± SEM, with individual data points shown as dots overlaid on the bar graphs. Statistical analysis by one-way ANOVA (ns, not significant; * P < 0.05, *** P < 0.001, **** P < 0.0001). H Representative images and quantification of single-colored MADM mouse cardiomyocytes. MADM cardiomyocytes were infected with Adeno- Tnnt2 -Cre adenovirus and then treated with DMSO, PE, HM or 2SM for 72 h. Scale bar, 100 μm. n = 3 per group. Data represent mean ± SEM, with individual data points shown as dots overlaid on the bar graphs. Statistical analysis by one-way ANOVA (ns, not significant; **** P < 0.0001)

    Article Snippet: Compounds and their working concentrations were as follows: phenylephrine hydrochloride (PE, TargetMol, T0453), 2 μmol/L; harmine (HM, MCE, HYN0737A), 5 μmol/L; baricitinib (BA, TargetMol, T2485), 4 μmol/L; Vo-ohpic trihydrate (VO, MCE, HY13074), 2 μmol/L; AZD3965 (AZ, MCE, HY12750), 2 μmol/L; rapamycin (MCE, HY10219), 20 nmol/L; and M2N12 (MCE, HY128769 ), 0.5 μmol/L.

    Techniques: In Vitro, Derivative Assay, Formulation, Infection, Control, Staining