biolog phenotype microarray screening platform  (Biolog Inc)

Journal: Biochemical Journal

Article Title: On the function of TRAP substrate-binding proteins: the isethionate-specific binding protein IseP

doi: 10.1042/BCJ20240540

Figure Lengend Snippet: ( A ) Thermal shift heatmap for Oa IseP displaying changes in melting temperature ( Δ T m D , °C) with various phosphate and sulfur metabolites. The mean from technical quadruplicates of each condition is shown. Isethionate (position H10) was the only hit ( Δ T m D > 2°C) amongst 94 metabolites from the Phenotype MicroArray PM4A screen. These data are also tabulated in . ( B ) Oa IseP binding curve and affinity ( K D ) for isethionate determined by ITC. This experiment was conducted with technical triplicates and a representative result is shown. When error bars are not apparent, they are smaller than the data points. These data are also tabulated in .

Article Snippet: For experiments using the Phenotype MicroArray PM4A Biolog screen, the compounds were dissolved in 50 μl of 50 mM Tris pH 8.0, 150 mM NaCl buffer, before 7.5 μl was used in each replicate (Biolog does not disclose the exact concentrations in their screens).

Techniques: Microarray, Binding Assay

Journal: Biochemical Journal

Article Title: On the function of TRAP substrate-binding proteins: the isethionate-specific binding protein IseP

doi: 10.1042/BCJ20240540

Figure Lengend Snippet: Thermal stability and isothermal titration calorimetry for isethionate binding

Article Snippet: For experiments using the Phenotype MicroArray PM4A Biolog screen, the compounds were dissolved in 50 μl of 50 mM Tris pH 8.0, 150 mM NaCl buffer, before 7.5 μl was used in each replicate (Biolog does not disclose the exact concentrations in their screens).

Techniques: Isothermal Titration Calorimetry, Control, Binding Assay