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phalloidin af488  (Thermo Fisher)


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    Structured Review

    Thermo Fisher phalloidin af488
    Phalloidin Af488, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phalloidin+buffer/BSA+blocking+buffer/pm40745192-201-44-47
    Average 99 stars, based on 1 article reviews
    phalloidin af488 - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    FACS:

    Article Title: Ex vivo allergen specific type 2 immune responses in house dust mite-driven acute airway inflammation in mice depend on allergen extract characteristics
    Article Snippet: 50 μL BALF cell suspension or 1 × 10 6 lung cells per well were plated in 96-well microplates (Falcon ® , Corning, USA) and centrifuged (300 g, 5 min, 4 °C). .. Cells were washed with PBS, followed by addition of Fixable Viability Dye (eFluor 780, Thermo Fisher Scientific, USA) for 30 min. After washing with FACS buffer, PBS blocking buffer containing 1% BSA, 5% FCS and 1% CD16/CD32 blocking solution (Thermo Fisher Scientific) was added for 15 min to block a-specific background. .. After another round of washing, the following antibodies were added: Panel 1: BALF cells - CD3e-Pe Cy7, MHCII-FITC, CD11c-APC, B220-Pe Cy7 (Thermo Fisher Scientific) and CCR3-PE (BioLegend, USA) and matching isotypes.

    Article Title: Ex vivo allergen specific type 2 immune responses in house dust mite-driven acute airway inflammation in mice depend on allergen extract characteristics.
    Article Snippet: 50 μL BALF cell suspension or 1 × 106 lung cells per well were plated in 96-well microplates (Falcon®, Corning, USA) and centrifuged (300 g, 5 min, 4 °C). .. Cells were washed with PBS, followed by addition of Fixable Viability Dye (eFluor 780, Thermo Fisher Scientific, USA) for 30 min. After washing with FACS buffer, PBS blocking buffer containing 1% BSA, 5% FCS and 1% CD16/CD32 blocking solution (Thermo Fisher Scientific) was added for 15 min to block a-specific background. .. After another round of washing, the following antibodies were added: Panel 1: BALF cells - CD3e-Pe Cy7, MHCII-FITC, CD11c-APC, B220-Pe Cy7 (Thermo Fisher Scientific) and CCR3-PE (BioLegend, USA) and matching isotypes.

    Blocking Assay:

    Article Title: Ex vivo allergen specific type 2 immune responses in house dust mite-driven acute airway inflammation in mice depend on allergen extract characteristics
    Article Snippet: 50 μL BALF cell suspension or 1 × 10 6 lung cells per well were plated in 96-well microplates (Falcon ® , Corning, USA) and centrifuged (300 g, 5 min, 4 °C). .. Cells were washed with PBS, followed by addition of Fixable Viability Dye (eFluor 780, Thermo Fisher Scientific, USA) for 30 min. After washing with FACS buffer, PBS blocking buffer containing 1% BSA, 5% FCS and 1% CD16/CD32 blocking solution (Thermo Fisher Scientific) was added for 15 min to block a-specific background. .. After another round of washing, the following antibodies were added: Panel 1: BALF cells - CD3e-Pe Cy7, MHCII-FITC, CD11c-APC, B220-Pe Cy7 (Thermo Fisher Scientific) and CCR3-PE (BioLegend, USA) and matching isotypes.

    Article Title: Computational and Proteomic Analyses Reveal Cardiac Dysfunction and Heart Failure-Associated Biomarker Secretion from Venezuelan Equine Encephalitis Virus TC83-infected human IPSC-derived cardiomyocytes
    Article Snippet: After migration, SDS-Page gel was transferred to a PVDF membrane (0.45 μm, ThermoFisher Scientific, Cat. 88518) with a wet system using the NuPAGETM Transfer Buffer (ThermoFisher Scientific, Cat. NP0006) and a blot module (ThermoFisher Scientific, Cat. B1000). .. After the transfer, the membrane was blocked for 1 hour at room temperature with a BSA blocking buffer (ThermoFisher Scientific, Cat. 37520), followed by primary antibody incubation (with a dilution 1:3,000) except for GAPDH (dilution 1:10,000) overnight at 4 °C or 2 hours at room temperature in 1% BSA blocking buffer. ..

    Article Title: Ex vivo allergen specific type 2 immune responses in house dust mite-driven acute airway inflammation in mice depend on allergen extract characteristics.
    Article Snippet: 50 μL BALF cell suspension or 1 × 106 lung cells per well were plated in 96-well microplates (Falcon®, Corning, USA) and centrifuged (300 g, 5 min, 4 °C). .. Cells were washed with PBS, followed by addition of Fixable Viability Dye (eFluor 780, Thermo Fisher Scientific, USA) for 30 min. After washing with FACS buffer, PBS blocking buffer containing 1% BSA, 5% FCS and 1% CD16/CD32 blocking solution (Thermo Fisher Scientific) was added for 15 min to block a-specific background. .. After another round of washing, the following antibodies were added: Panel 1: BALF cells - CD3e-Pe Cy7, MHCII-FITC, CD11c-APC, B220-Pe Cy7 (Thermo Fisher Scientific) and CCR3-PE (BioLegend, USA) and matching isotypes.

    Article Title: Single-nucleus brain transcriptomics reveals microglia dysfunction in multiple system atrophy.
    Article Snippet: The tissue was homogenized in a precooled Dounce homogenizer containing ice-cooled homogenization buffer (250 mM sucrose, 25 mM KCl, 5 mM MgCl2, 10 mM Tris buffer, pH 8.0, 1 μM DTT, 1x protease inhibitor (Roche, Switzerland), 0.4 U/μL RNAse inhibitor (Takara, USA), 0.2 U/μL Superasin inhibitor (Invitrogen, USA), and 0.1% Triton X-100) using both loose and tight pestles before filtering using a 70 μm filter (Millipore). .. Nuclei were collected (4 °C, 1,000 rcf, 9 min) and the pellet resuspended in a blocking buffer (RNase-free PBS, 0.5% BSA (VWR, USA) and 0.2 U/μL RNase inhibitor (Takara, USA)) containing 2 μg/mL 7-amino actinomycin D (7-AAD; ThermoFisher Scientific) to stain nuclei before sorting on a BD FACSAria III sorter using a 75 μm nozzle and controlled by BD FACSDiva 8.0.1 software. ..

    Article Title: A senescent iCAF-like fibroblast state governs therapy resistance in rheumatoid arthritis
    Article Snippet: Equal amounts of protein (10 μg) were separated by SDS-PAGE on 4-15% gradient precast gels (Bio-Rad, 4561086) using Tris-glycine-SDS running buffer (Nippon Gene, 312-90321) and subsequently transferred onto PVDF membranes (Millipore, IPVH10100). .. Membranes were blocked with BSA-based blocking solution (Thermo Fisher Scientific, 37520) and incubated overnight at 4 °C with primary antibodies diluted in TBS containing 3% BSA. .. The following primary antibodies were used at a dilution of 1:1,000: anti-STAT3 (clone: D3Z2G, Cell Signaling Technology, 9145), anti-phospho-STAT3 (clone: EP2147Y, Abcam, ab76315), and anti-actin (clone: 13E5, Cell Signaling Technology, 4970S).

    Article Title: Differential Expression of LncRNA NEAT1 in 3D Tumoroids Compared to 2D Cultures Highlights Its Role in Glioblastoma Progression.
    Article Snippet: NEAT1 (Nuclear-Enriched Abundant Transcript1) is a long non-coding RNA (lncRNA) that critically regulates tumorigenesis, with growing recognition of its potential as a therapeutic target.. However, most functional in vitro studies of lncRNAs rely on 2D cell culture systems, which lack the architectural and physiological complexity of tumors.. Here, we demonstrate that 3D tumor architecture reshapes lncRNA-driven oncogenic programs.

    Article Title: Microfluidic toolbox using padlock probes and rolling circle amplification for direct detection and genotyping of viral RNA
    Article Snippet: .. 1% BSA blocking solution in PBS (Thermo Fisher) was also flowed at 5 μL min −1 for 10 min right before starting the on-chip RCA experiments. ..

    Membrane:

    Article Title: Computational and Proteomic Analyses Reveal Cardiac Dysfunction and Heart Failure-Associated Biomarker Secretion from Venezuelan Equine Encephalitis Virus TC83-infected human IPSC-derived cardiomyocytes
    Article Snippet: After migration, SDS-Page gel was transferred to a PVDF membrane (0.45 μm, ThermoFisher Scientific, Cat. 88518) with a wet system using the NuPAGETM Transfer Buffer (ThermoFisher Scientific, Cat. NP0006) and a blot module (ThermoFisher Scientific, Cat. B1000). .. After the transfer, the membrane was blocked for 1 hour at room temperature with a BSA blocking buffer (ThermoFisher Scientific, Cat. 37520), followed by primary antibody incubation (with a dilution 1:3,000) except for GAPDH (dilution 1:10,000) overnight at 4 °C or 2 hours at room temperature in 1% BSA blocking buffer. ..

    Incubation:

    Article Title: Computational and Proteomic Analyses Reveal Cardiac Dysfunction and Heart Failure-Associated Biomarker Secretion from Venezuelan Equine Encephalitis Virus TC83-infected human IPSC-derived cardiomyocytes
    Article Snippet: After migration, SDS-Page gel was transferred to a PVDF membrane (0.45 μm, ThermoFisher Scientific, Cat. 88518) with a wet system using the NuPAGETM Transfer Buffer (ThermoFisher Scientific, Cat. NP0006) and a blot module (ThermoFisher Scientific, Cat. B1000). .. After the transfer, the membrane was blocked for 1 hour at room temperature with a BSA blocking buffer (ThermoFisher Scientific, Cat. 37520), followed by primary antibody incubation (with a dilution 1:3,000) except for GAPDH (dilution 1:10,000) overnight at 4 °C or 2 hours at room temperature in 1% BSA blocking buffer. ..

    Article Title: A senescent iCAF-like fibroblast state governs therapy resistance in rheumatoid arthritis
    Article Snippet: Equal amounts of protein (10 μg) were separated by SDS-PAGE on 4-15% gradient precast gels (Bio-Rad, 4561086) using Tris-glycine-SDS running buffer (Nippon Gene, 312-90321) and subsequently transferred onto PVDF membranes (Millipore, IPVH10100). .. Membranes were blocked with BSA-based blocking solution (Thermo Fisher Scientific, 37520) and incubated overnight at 4 °C with primary antibodies diluted in TBS containing 3% BSA. .. The following primary antibodies were used at a dilution of 1:1,000: anti-STAT3 (clone: D3Z2G, Cell Signaling Technology, 9145), anti-phospho-STAT3 (clone: EP2147Y, Abcam, ab76315), and anti-actin (clone: 13E5, Cell Signaling Technology, 4970S).

    Article Title: Differential Expression of LncRNA NEAT1 in 3D Tumoroids Compared to 2D Cultures Highlights Its Role in Glioblastoma Progression.
    Article Snippet: NEAT1 (Nuclear-Enriched Abundant Transcript1) is a long non-coding RNA (lncRNA) that critically regulates tumorigenesis, with growing recognition of its potential as a therapeutic target.. However, most functional in vitro studies of lncRNAs rely on 2D cell culture systems, which lack the architectural and physiological complexity of tumors.. Here, we demonstrate that 3D tumor architecture reshapes lncRNA-driven oncogenic programs.

    Staining:

    Article Title: Single-nucleus brain transcriptomics reveals microglia dysfunction in multiple system atrophy.
    Article Snippet: The tissue was homogenized in a precooled Dounce homogenizer containing ice-cooled homogenization buffer (250 mM sucrose, 25 mM KCl, 5 mM MgCl2, 10 mM Tris buffer, pH 8.0, 1 μM DTT, 1x protease inhibitor (Roche, Switzerland), 0.4 U/μL RNAse inhibitor (Takara, USA), 0.2 U/μL Superasin inhibitor (Invitrogen, USA), and 0.1% Triton X-100) using both loose and tight pestles before filtering using a 70 μm filter (Millipore). .. Nuclei were collected (4 °C, 1,000 rcf, 9 min) and the pellet resuspended in a blocking buffer (RNase-free PBS, 0.5% BSA (VWR, USA) and 0.2 U/μL RNase inhibitor (Takara, USA)) containing 2 μg/mL 7-amino actinomycin D (7-AAD; ThermoFisher Scientific) to stain nuclei before sorting on a BD FACSAria III sorter using a 75 μm nozzle and controlled by BD FACSDiva 8.0.1 software. ..

    Software:

    Article Title: Single-nucleus brain transcriptomics reveals microglia dysfunction in multiple system atrophy.
    Article Snippet: The tissue was homogenized in a precooled Dounce homogenizer containing ice-cooled homogenization buffer (250 mM sucrose, 25 mM KCl, 5 mM MgCl2, 10 mM Tris buffer, pH 8.0, 1 μM DTT, 1x protease inhibitor (Roche, Switzerland), 0.4 U/μL RNAse inhibitor (Takara, USA), 0.2 U/μL Superasin inhibitor (Invitrogen, USA), and 0.1% Triton X-100) using both loose and tight pestles before filtering using a 70 μm filter (Millipore). .. Nuclei were collected (4 °C, 1,000 rcf, 9 min) and the pellet resuspended in a blocking buffer (RNase-free PBS, 0.5% BSA (VWR, USA) and 0.2 U/μL RNase inhibitor (Takara, USA)) containing 2 μg/mL 7-amino actinomycin D (7-AAD; ThermoFisher Scientific) to stain nuclei before sorting on a BD FACSAria III sorter using a 75 μm nozzle and controlled by BD FACSDiva 8.0.1 software. ..

    Marker:

    Article Title: Differential Expression of LncRNA NEAT1 in 3D Tumoroids Compared to 2D Cultures Highlights Its Role in Glioblastoma Progression.
    Article Snippet: NEAT1 (Nuclear-Enriched Abundant Transcript1) is a long non-coding RNA (lncRNA) that critically regulates tumorigenesis, with growing recognition of its potential as a therapeutic target.. However, most functional in vitro studies of lncRNAs rely on 2D cell culture systems, which lack the architectural and physiological complexity of tumors.. Here, we demonstrate that 3D tumor architecture reshapes lncRNA-driven oncogenic programs.



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    Controls for rapalog motility experiments and perimitochondrial actin recruitment. (A) Representative example neurites showing rapalog recruits mCherry-FRB to mitochondria in the presence of vehicle or AntA. Neurons were transfected with mito-GFP-FKBP and mCherry-FRB and pretreated for 1 h with either 4 nM AntA or vehicle before addition of 1 µM rapalog as in . (B) Representative images of hippocampal neurons that were fixed and stained with Alexa Fluor 488 <t>phalloidin</t> conjugate and Hoechst. Either control neurons or neurons pretreated with 4 nM AntA were exposed to 5 µM LatA. Note the persistence of a LatA-resistant pool of actin after pretreatment with AntA. (C) Quantification of filamentous actin from the average Alexa Fluor 488 phalloidin intensity of 60× imaging fields of neurons treated as in B. (D) Quantification of mitochondrial motility in hippocampal neurons pretreated for 1 h with 4 nM AntA and then 30 min with both 5 uM LatA and 4 nM AntA. Once arrested by treatment with AntA, LatA could not reverse the arrest. (E) Time-lapse series of fibroblasts treated for 1 h with 40 nM AntA and imaged as in . The arrowhead indicates a mitochondrion that appears to lack perimitochondrial actin and moves, while nearby mitochondria do not. (F) Representative images of rat embryonic fibroblasts transduced with GFP-F-tractin and mito-mRaspberry from the quantification in . Cells cultured in galactose were treated with 40 nM AntA or vehicle. One hour after drug treatment, cells either had glucose or galactose added to a concentration of 25 mM, or no further addition. Cells were imaged after 30 min. P values for C were calculated by two-tailed, unpaired t tests with Welch’s correction. A blocked one-way ANOVA was performed with Tukey’s multiple comparison correction for D. Select comparisons are shown for D. For supplemental images, 3A scale bars = 10 µm. The scale bar in 3E is 5 µm. For supplemental images, 3B and 3F scale bars = 20 μm.
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    Image Search Results


    Controls for rapalog motility experiments and perimitochondrial actin recruitment. (A) Representative example neurites showing rapalog recruits mCherry-FRB to mitochondria in the presence of vehicle or AntA. Neurons were transfected with mito-GFP-FKBP and mCherry-FRB and pretreated for 1 h with either 4 nM AntA or vehicle before addition of 1 µM rapalog as in . (B) Representative images of hippocampal neurons that were fixed and stained with Alexa Fluor 488 phalloidin conjugate and Hoechst. Either control neurons or neurons pretreated with 4 nM AntA were exposed to 5 µM LatA. Note the persistence of a LatA-resistant pool of actin after pretreatment with AntA. (C) Quantification of filamentous actin from the average Alexa Fluor 488 phalloidin intensity of 60× imaging fields of neurons treated as in B. (D) Quantification of mitochondrial motility in hippocampal neurons pretreated for 1 h with 4 nM AntA and then 30 min with both 5 uM LatA and 4 nM AntA. Once arrested by treatment with AntA, LatA could not reverse the arrest. (E) Time-lapse series of fibroblasts treated for 1 h with 40 nM AntA and imaged as in . The arrowhead indicates a mitochondrion that appears to lack perimitochondrial actin and moves, while nearby mitochondria do not. (F) Representative images of rat embryonic fibroblasts transduced with GFP-F-tractin and mito-mRaspberry from the quantification in . Cells cultured in galactose were treated with 40 nM AntA or vehicle. One hour after drug treatment, cells either had glucose or galactose added to a concentration of 25 mM, or no further addition. Cells were imaged after 30 min. P values for C were calculated by two-tailed, unpaired t tests with Welch’s correction. A blocked one-way ANOVA was performed with Tukey’s multiple comparison correction for D. Select comparisons are shown for D. For supplemental images, 3A scale bars = 10 µm. The scale bar in 3E is 5 µm. For supplemental images, 3B and 3F scale bars = 20 μm.

    Journal: The Journal of Cell Biology

    Article Title: Energy stress activates AMPK to arrest mitochondria via phosphorylation of TRAK1

    doi: 10.1083/jcb.202501023

    Figure Lengend Snippet: Controls for rapalog motility experiments and perimitochondrial actin recruitment. (A) Representative example neurites showing rapalog recruits mCherry-FRB to mitochondria in the presence of vehicle or AntA. Neurons were transfected with mito-GFP-FKBP and mCherry-FRB and pretreated for 1 h with either 4 nM AntA or vehicle before addition of 1 µM rapalog as in . (B) Representative images of hippocampal neurons that were fixed and stained with Alexa Fluor 488 phalloidin conjugate and Hoechst. Either control neurons or neurons pretreated with 4 nM AntA were exposed to 5 µM LatA. Note the persistence of a LatA-resistant pool of actin after pretreatment with AntA. (C) Quantification of filamentous actin from the average Alexa Fluor 488 phalloidin intensity of 60× imaging fields of neurons treated as in B. (D) Quantification of mitochondrial motility in hippocampal neurons pretreated for 1 h with 4 nM AntA and then 30 min with both 5 uM LatA and 4 nM AntA. Once arrested by treatment with AntA, LatA could not reverse the arrest. (E) Time-lapse series of fibroblasts treated for 1 h with 40 nM AntA and imaged as in . The arrowhead indicates a mitochondrion that appears to lack perimitochondrial actin and moves, while nearby mitochondria do not. (F) Representative images of rat embryonic fibroblasts transduced with GFP-F-tractin and mito-mRaspberry from the quantification in . Cells cultured in galactose were treated with 40 nM AntA or vehicle. One hour after drug treatment, cells either had glucose or galactose added to a concentration of 25 mM, or no further addition. Cells were imaged after 30 min. P values for C were calculated by two-tailed, unpaired t tests with Welch’s correction. A blocked one-way ANOVA was performed with Tukey’s multiple comparison correction for D. Select comparisons are shown for D. For supplemental images, 3A scale bars = 10 µm. The scale bar in 3E is 5 µm. For supplemental images, 3B and 3F scale bars = 20 μm.

    Article Snippet: The phalloidin buffer was washed off, and the sample was incubated for 30 min with AF488-conjugated phalloidin (sc-36379; Santa Cruz) dissolved in 1:1,000 in phalloidin buffer.

    Techniques: Transfection, Staining, Control, Imaging, Transduction, Cell Culture, Concentration Assay, Two Tailed Test, Comparison

    a) Schematic and photographic overview of the rat tail anatomy highlighting the location of interfascicular matrix (IFM) and fascicular matrix (FM) regions within the tendon, followed by a 3D schematic of bundle structure. b) Immunofluorescence of a rat tail quadrant (longitudinal cuts) stained with DAPI (nucleus) and phalloidin (actin cytoskeleton). IFM and FM regions are visually evident with different morphology and organisation of IFM and FM cells. c) The schematic depicts a transverse plane through an isolated fascicle surrounded by IFM as on removal from a tail tendon. Confocal images through the fascicle at different heights (shown on the left) demonstrate that it is feasible to detect when imaging within the IFM or FM region. Fascicles were digested in collagenase for 0 – 120 minutes, fixed, stained and imaged, and the thickness of the remaining IFM recorded at different time intervals, confirmed that IFM was consistently fully digested after 120 minutes. d) The digestion protocol was selected based on these data, discarding the digested cells collected between 90 minutes and 150 minutes to ensure IFM and FM digestions remained distinct for each cell population (n=3 rats, 2 fascicles/rat).

    Journal: bioRxiv

    Article Title: Unravelling the distinct phenotype and mechanosensitive properties of different tendon cell populations

    doi: 10.64898/2026.01.08.698354

    Figure Lengend Snippet: a) Schematic and photographic overview of the rat tail anatomy highlighting the location of interfascicular matrix (IFM) and fascicular matrix (FM) regions within the tendon, followed by a 3D schematic of bundle structure. b) Immunofluorescence of a rat tail quadrant (longitudinal cuts) stained with DAPI (nucleus) and phalloidin (actin cytoskeleton). IFM and FM regions are visually evident with different morphology and organisation of IFM and FM cells. c) The schematic depicts a transverse plane through an isolated fascicle surrounded by IFM as on removal from a tail tendon. Confocal images through the fascicle at different heights (shown on the left) demonstrate that it is feasible to detect when imaging within the IFM or FM region. Fascicles were digested in collagenase for 0 – 120 minutes, fixed, stained and imaged, and the thickness of the remaining IFM recorded at different time intervals, confirmed that IFM was consistently fully digested after 120 minutes. d) The digestion protocol was selected based on these data, discarding the digested cells collected between 90 minutes and 150 minutes to ensure IFM and FM digestions remained distinct for each cell population (n=3 rats, 2 fascicles/rat).

    Article Snippet: Blocking was performed using a 1% BSA solution for 1 hour, before cells were stained with phalloidin (Texas RedTM-X Phalloidin ThermoFisher diluted in 1% BSA solution) for an additional 45 minutes, followed by three PBS rinses.

    Techniques: Immunofluorescence, Staining, Isolation, Imaging

    a) IFM and FM cells fixed and stained with DAPI (nucleus) and phalloidin (actin), comparing cell morphology at day 2 of P1 and P3. b) Comparison of mean cell area, cell nucleus area and actin expression/cell at P0, P2 and P3 (N=200/group, mean ± S.E.), comparing IFM cells (green) and FM cells (red). ****p<0.001. Further associated data is provided in .

    Journal: bioRxiv

    Article Title: Unravelling the distinct phenotype and mechanosensitive properties of different tendon cell populations

    doi: 10.64898/2026.01.08.698354

    Figure Lengend Snippet: a) IFM and FM cells fixed and stained with DAPI (nucleus) and phalloidin (actin), comparing cell morphology at day 2 of P1 and P3. b) Comparison of mean cell area, cell nucleus area and actin expression/cell at P0, P2 and P3 (N=200/group, mean ± S.E.), comparing IFM cells (green) and FM cells (red). ****p<0.001. Further associated data is provided in .

    Article Snippet: Blocking was performed using a 1% BSA solution for 1 hour, before cells were stained with phalloidin (Texas RedTM-X Phalloidin ThermoFisher diluted in 1% BSA solution) for an additional 45 minutes, followed by three PBS rinses.

    Techniques: Staining, Comparison, Expressing

    a) Typical images of IFM and FM cells at day 2 of P3 culture comparing collagen-coated TCP with 900 kPa and 20kPa surfaces, illustrating the morphological recovery. Cells are stained with DAPI (nucleus; blue) and Phalloidin (cytoskeleton, red). b) Image semi-quantification of changes in cell body area, nucleus area, and actin expression/cell at P3 of culture on substrates of varying stiffness. Mean ± S.E. of >200 cells/condition are displayed for IFM cells (green) and FM cells (red). *= p<0.001 highlights a significant difference between the condition and the P0 TCP reference. A more detailed breakdown of the distribution of cell morphologies for each condition and correlations between cell body area, nucleus area and actin expression/cell are shown in & .

    Journal: bioRxiv

    Article Title: Unravelling the distinct phenotype and mechanosensitive properties of different tendon cell populations

    doi: 10.64898/2026.01.08.698354

    Figure Lengend Snippet: a) Typical images of IFM and FM cells at day 2 of P3 culture comparing collagen-coated TCP with 900 kPa and 20kPa surfaces, illustrating the morphological recovery. Cells are stained with DAPI (nucleus; blue) and Phalloidin (cytoskeleton, red). b) Image semi-quantification of changes in cell body area, nucleus area, and actin expression/cell at P3 of culture on substrates of varying stiffness. Mean ± S.E. of >200 cells/condition are displayed for IFM cells (green) and FM cells (red). *= p<0.001 highlights a significant difference between the condition and the P0 TCP reference. A more detailed breakdown of the distribution of cell morphologies for each condition and correlations between cell body area, nucleus area and actin expression/cell are shown in & .

    Article Snippet: Blocking was performed using a 1% BSA solution for 1 hour, before cells were stained with phalloidin (Texas RedTM-X Phalloidin ThermoFisher diluted in 1% BSA solution) for an additional 45 minutes, followed by three PBS rinses.

    Techniques: Staining, Expressing