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Promega phage vector lambda embl3
Phage Vector Lambda Embl3, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phage+vector+lambda+embl3/embl3/pmc02915743-194-30-34
Average 90 stars, based on 1 article reviews
phage vector lambda embl3 - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Clone Assay:

Article Title: Isolation of Sporothrix schenckii GDA1 and functional characterization of the encoded guanosine diphosphatase activity.
Article Snippet: The dimorphic fungus Sporothrix schenckii is the etiological agent of sporotrichosis, the rose-picker’s disease.. This is a subcutaneous mycosis distributed worldwide, affecting people with occupations related to soil handling (LopezAvalos et al. 2001; Lopes-Bezerra et al. 2006), and mainly caused by traumatic inoculation of the fungus, although inhalation of conidia can cause pulmonary sporotrichosis (Ramos-e-Silva et al. 2007).. The fungal wall protects the cell from drastic changes in the external environment, which is the first point of contact with the host cells and a main source of pathogen-associated molecular patterns recognized by the host immune system (Mora-Montes et al. 2009).

other:

Article Title: Transferrin receptor genes of
Article Snippet: Size-fractionated chromosomal DNA was ligated with BamHI-digested EMBL3 arms (Promega), using T4 DNA ligase in a final volume of 9 μL.

Article Title: The gene coding for a new transcription factor (ftf1) of Fusarium oxysporum is only expressed during infection of common bean.
Article Snippet: We report the isolation and analysis of the gene encoding ftf1 (Fusarium transcription factor 1), a previously undescribed putative transcription factor from highly virulent strains of Fusarium oxysporum f.sp. phaseoli that is transcribed specifically during early stages of infection of its host common bean (Phaseolus vulgaris L.).. The predicted 1080 amino acid ftf1 protein contains a Zn(II)2-Cys6 binuclear cluster DNA-binding motif. ftf1 expression during axenic growth in culture was not detected by either Northern or RT-PCR.. On the contrary, in planta transcription of ftf1 is increased about 24 h after plant inoculation, as detected by real-time RT-PCR.

Article Title: Alkane inducible proteins in Geobacillus thermoleovorans B23
Article Snippet: Then the DNA fragments were ligated with phage vector Lambda EMBL3 using Lambda EMBL3/ Bam HI arm (Promega, Madison, WI) and packaged in vitro by Maxplax packaging extract kit (Epicentre Technologies, Madison, WI).

Plasmid Preparation:

Article Title: Perilla-derived promoter functioning in petals
Article Snippet: This chromosomal DNA was partially decomposed with Sau3AI (Toyobo), and a fraction containing a 10 kb to 15 kb DNA fragment was recovered using a sucrose density gradient method. .. This fragment was then inserted into the BamHI site of EMBL3 (Promega), a type of lambda phage vector, using a known method to prepare a chromosomal DNA library. ..

Article Title: Estrogen-dependent Transcription of the NEL-like 2 ( NELL2 ) Gene and Its Role in Protection from Cell Death
Article Snippet: .. A mouse genomic DNA library (Invitrogen) was constructed in Escherichia coli LE392 after ligating partial Sau3AI DNA digests of mouse genomic DNA (strain 129/Sv) at the BamHI site of a phage vector lambda EMBL3 (Promega, Madison, WI). .. For screening clones containing the m NELL2 gene, ∼1 × 10 7 phage plaques were transferred to Hybond N + membrane (Amersham Biosciences) and hybridized to a [α- 32 P]dCTP (50 μCi/μl, Amersham Biosciences)-labeled m NELL2 cDNA probe; the cDNA fragment (a 680-bp fragment spanning nucleotides 21–700 in m NELL2 mRNA) was cloned by PCR amplification based on the deposited DNA sequence (NCBI GenBank TM accession number {"type":"entrez-nucleotide","attrs":{"text":"NM_016743","term_id":"133922560","term_text":"NM_016743"}} NM_016743 ).

Article Title: Method for production of chrysanthemum plant having delphinidin-containing petals
Article Snippet: This chromosomal DNA was partially decomposed with Sau3AI (Toyobo), and a fraction containing a 10 kb to 15 kb DNA fragment was recovered using a sucrose density gradient method. .. This fragment was then inserted into the BamHI site of EMBL3 (Promega), a type of lambda phage vector, using a known method to prepare a genomic DNA library. ..

Construct:

Article Title: Estrogen-dependent Transcription of the NEL-like 2 ( NELL2 ) Gene and Its Role in Protection from Cell Death
Article Snippet: .. A mouse genomic DNA library (Invitrogen) was constructed in Escherichia coli LE392 after ligating partial Sau3AI DNA digests of mouse genomic DNA (strain 129/Sv) at the BamHI site of a phage vector lambda EMBL3 (Promega, Madison, WI). .. For screening clones containing the m NELL2 gene, ∼1 × 10 7 phage plaques were transferred to Hybond N + membrane (Amersham Biosciences) and hybridized to a [α- 32 P]dCTP (50 μCi/μl, Amersham Biosciences)-labeled m NELL2 cDNA probe; the cDNA fragment (a 680-bp fragment spanning nucleotides 21–700 in m NELL2 mRNA) was cloned by PCR amplification based on the deposited DNA sequence (NCBI GenBank TM accession number {"type":"entrez-nucleotide","attrs":{"text":"NM_016743","term_id":"133922560","term_text":"NM_016743"}} NM_016743 ).



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