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lambda gt11 phage  (TaKaRa)


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    Structured Review

    TaKaRa lambda gt11 phage
    Lambda Gt11 Phage, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phage+lambda+gt11/us07329499-40-17-20
    Average 86 stars, based on 1 article reviews
    lambda gt11 phage - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Expressed ligand-vascular intercellular signaling molecule
    Article Snippet: .. A PCR band corresponding to TL3 was identified in a mouse uterus cDNA library in lambda gt-11. (Clontech). ..

    Article Title: Immediate response of myocardium to pressure overload includes transient regulation of genes associated with mitochondrial bioenergetics and calcium availability
    Article Snippet: The 5’ STRETCH PLUS rat heart cDNA Library (Clontech, USA) was used for isolation and purifica- tion of clones from phage lysis plates according to the manufacturer’s instructions. .. DNA amplification was made by PCR in 96-well microplates using the primers gt11 LD-Insert Screening Amplimers (Clontech, USA). ..

    cDNA Library Assay:

    Article Title: Expressed ligand-vascular intercellular signaling molecule
    Article Snippet: .. A PCR band corresponding to TL3 was identified in a mouse uterus cDNA library in lambda gt-11. (Clontech). ..

    Article Title: Human dopamine receptor and its uses
    Article Snippet: .. A human substantia nigra cDNA library construced in lambda gt11 (Clontech) and a pituitary cDNA library constructed in lambda gt10 as described in Example 2 were screened for clones encoding the D4 receptor. .. Approximately 0.5-1×106 plaque-forming units (p.f.u.) were transferred in duplicate to nylon filters (DuPont/NEN) and probed with a 32P-labeled 700 bp EcoRI-XhoI fragment encoding the cDNA isolated from the neuroepithelioma SK-N-MC under conditions as described in Example 2 above.

    Article Title: Reconstitution of functional tight junctions with individual claudin subtypes in epithelial cells.
    Article Snippet: .. Using each of the DNA fragments as a hybridization probe, a gt11 dog liver cDNA library (Clontech Laboratories, Inc.) was screened, and the obtained cDNA fragments containing the ORF of claudin-1 or claudin-4 were cloned into pBluescript SK(-). .. The full-length ORFs were amplified by PCR using KOD-Plus-Ver.2 DNA polymerase (TOYOBO) and subcloned into a mammalian expression vector, pCAGGS containing a neomycin-resistant gene (Niwa et al., 1991).

    Article Title: Use of a novel human ULIP6/CRMP protein in diagnosis of paraneoplastic neurological syndromes
    Article Snippet: .. In order to obtain a complete Ulip6 cDNA, the human spinal cord cDNA library, cloned into the lambda gt11 phage (Clontech, Palo Alto, U.S.A.), was screened with a radioactive probe. ..

    Article Title: A nonessential role for Arg 55 in cyclophilin18 for catalysis of proline isomerization during protein folding
    Article Snippet: .. Cloning, production, and purification of Cyp18 and Cyp18 R55A The human cyclophilin A (Cyp18) gene was isolated from a human lymphocyte cDNA library (CD4 positive) in the cloning vector lambda gt11 from Clontech and cloned into the expression vector, pACA. ..

    Construct:

    Article Title: Human dopamine receptor and its uses
    Article Snippet: .. A human substantia nigra cDNA library construced in lambda gt11 (Clontech) and a pituitary cDNA library constructed in lambda gt10 as described in Example 2 were screened for clones encoding the D4 receptor. .. Approximately 0.5-1×106 plaque-forming units (p.f.u.) were transferred in duplicate to nylon filters (DuPont/NEN) and probed with a 32P-labeled 700 bp EcoRI-XhoI fragment encoding the cDNA isolated from the neuroepithelioma SK-N-MC under conditions as described in Example 2 above.

    Clone Assay:

    Article Title: Human dopamine receptor and its uses
    Article Snippet: .. A human substantia nigra cDNA library construced in lambda gt11 (Clontech) and a pituitary cDNA library constructed in lambda gt10 as described in Example 2 were screened for clones encoding the D4 receptor. .. Approximately 0.5-1×106 plaque-forming units (p.f.u.) were transferred in duplicate to nylon filters (DuPont/NEN) and probed with a 32P-labeled 700 bp EcoRI-XhoI fragment encoding the cDNA isolated from the neuroepithelioma SK-N-MC under conditions as described in Example 2 above.

    Article Title: Reconstitution of functional tight junctions with individual claudin subtypes in epithelial cells.
    Article Snippet: .. Using each of the DNA fragments as a hybridization probe, a gt11 dog liver cDNA library (Clontech Laboratories, Inc.) was screened, and the obtained cDNA fragments containing the ORF of claudin-1 or claudin-4 were cloned into pBluescript SK(-). .. The full-length ORFs were amplified by PCR using KOD-Plus-Ver.2 DNA polymerase (TOYOBO) and subcloned into a mammalian expression vector, pCAGGS containing a neomycin-resistant gene (Niwa et al., 1991).

    Article Title: Use of a novel human ULIP6/CRMP protein in diagnosis of paraneoplastic neurological syndromes
    Article Snippet: .. In order to obtain a complete Ulip6 cDNA, the human spinal cord cDNA library, cloned into the lambda gt11 phage (Clontech, Palo Alto, U.S.A.), was screened with a radioactive probe. ..

    Article Title: A nonessential role for Arg 55 in cyclophilin18 for catalysis of proline isomerization during protein folding
    Article Snippet: .. Cloning, production, and purification of Cyp18 and Cyp18 R55A The human cyclophilin A (Cyp18) gene was isolated from a human lymphocyte cDNA library (CD4 positive) in the cloning vector lambda gt11 from Clontech and cloned into the expression vector, pACA. ..

    Hybridization:

    Article Title: Reconstitution of functional tight junctions with individual claudin subtypes in epithelial cells.
    Article Snippet: .. Using each of the DNA fragments as a hybridization probe, a gt11 dog liver cDNA library (Clontech Laboratories, Inc.) was screened, and the obtained cDNA fragments containing the ORF of claudin-1 or claudin-4 were cloned into pBluescript SK(-). .. The full-length ORFs were amplified by PCR using KOD-Plus-Ver.2 DNA polymerase (TOYOBO) and subcloned into a mammalian expression vector, pCAGGS containing a neomycin-resistant gene (Niwa et al., 1991).

    Expressing:

    Article Title: Co‐chaperone BAG3 and adenovirus penton base protein partnership
    Article Snippet: GST‐BAG3, GST‐BAG3ΔC and GST expressed in DH5α grown at 16°C and induced with 0.5 mM IPTG overnight, were purified from a sonicated bacteria pellet on a glutathione–sepharose column (Amersham Biosciences), using for elution 10 mM glutathione in 50 mM Tris, pH 7.5, containing 200 mM NaCl. .. A human lung cDNA expression library in lambda gt11 (Clontech) was used throughout this work. ..

    Article Title: A nonessential role for Arg 55 in cyclophilin18 for catalysis of proline isomerization during protein folding
    Article Snippet: .. Cloning, production, and purification of Cyp18 and Cyp18 R55A The human cyclophilin A (Cyp18) gene was isolated from a human lymphocyte cDNA library (CD4 positive) in the cloning vector lambda gt11 from Clontech and cloned into the expression vector, pACA. ..

    Cloning:

    Article Title: A nonessential role for Arg 55 in cyclophilin18 for catalysis of proline isomerization during protein folding
    Article Snippet: .. Cloning, production, and purification of Cyp18 and Cyp18 R55A The human cyclophilin A (Cyp18) gene was isolated from a human lymphocyte cDNA library (CD4 positive) in the cloning vector lambda gt11 from Clontech and cloned into the expression vector, pACA. ..

    Purification:

    Article Title: A nonessential role for Arg 55 in cyclophilin18 for catalysis of proline isomerization during protein folding
    Article Snippet: .. Cloning, production, and purification of Cyp18 and Cyp18 R55A The human cyclophilin A (Cyp18) gene was isolated from a human lymphocyte cDNA library (CD4 positive) in the cloning vector lambda gt11 from Clontech and cloned into the expression vector, pACA. ..

    Isolation:

    Article Title: A nonessential role for Arg 55 in cyclophilin18 for catalysis of proline isomerization during protein folding
    Article Snippet: .. Cloning, production, and purification of Cyp18 and Cyp18 R55A The human cyclophilin A (Cyp18) gene was isolated from a human lymphocyte cDNA library (CD4 positive) in the cloning vector lambda gt11 from Clontech and cloned into the expression vector, pACA. ..

    Plasmid Preparation:

    Article Title: A nonessential role for Arg 55 in cyclophilin18 for catalysis of proline isomerization during protein folding
    Article Snippet: .. Cloning, production, and purification of Cyp18 and Cyp18 R55A The human cyclophilin A (Cyp18) gene was isolated from a human lymphocyte cDNA library (CD4 positive) in the cloning vector lambda gt11 from Clontech and cloned into the expression vector, pACA. ..

    DNA Amplification:

    Article Title: Immediate response of myocardium to pressure overload includes transient regulation of genes associated with mitochondrial bioenergetics and calcium availability
    Article Snippet: The 5’ STRETCH PLUS rat heart cDNA Library (Clontech, USA) was used for isolation and purifica- tion of clones from phage lysis plates according to the manufacturer’s instructions. .. DNA amplification was made by PCR in 96-well microplates using the primers gt11 LD-Insert Screening Amplimers (Clontech, USA). ..



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