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s65t gfp gene from pgreenlantern  (Thermo Fisher)


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    Structured Review

    Thermo Fisher s65t gfp gene from pgreenlantern
    S65t Gfp Gene From Pgreenlantern, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgreenlantern+(gfp)/pm11312669-131-5-6
    Average 90 stars, based on 1 article reviews
    s65t gfp gene from pgreenlantern - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Type I interferon response against viral and non-viral gene transfer in human tumor and primary cell lines.
    Article Snippet: 1A.. I. Virtanen Institute for Molecular Sciences, Department of Biotechnology and Molecular Medicine, University of Kuopio, P.O.. Box 1627, FIN-70211 Kuopio, Finland 2Department of Viral Diseases and Immunology, National Public Health Institute, FIN-00300 Helsinki, Finland 3Åbo Akademi University, Department of Biochemistry and Pharmacy, FIN-20520 Turku, Finland 4Gene Therapy Unit, Kuopio University Hospital, Kuopio, Finland

    In Vitro:

    Article Title: Type I interferon response against viral and non-viral gene transfer in human tumor and primary cell lines.
    Article Snippet: 1A.. I. Virtanen Institute for Molecular Sciences, Department of Biotechnology and Molecular Medicine, University of Kuopio, P.O.. Box 1627, FIN-70211 Kuopio, Finland 2Department of Viral Diseases and Immunology, National Public Health Institute, FIN-00300 Helsinki, Finland 3Åbo Akademi University, Department of Biochemistry and Pharmacy, FIN-20520 Turku, Finland 4Gene Therapy Unit, Kuopio University Hospital, Kuopio, Finland

    Plasmid Preparation:

    Article Title: Type I interferon response against viral and non-viral gene transfer in human tumor and primary cell lines.
    Article Snippet: 1A.. I. Virtanen Institute for Molecular Sciences, Department of Biotechnology and Molecular Medicine, University of Kuopio, P.O.. Box 1627, FIN-70211 Kuopio, Finland 2Department of Viral Diseases and Immunology, National Public Health Institute, FIN-00300 Helsinki, Finland 3Åbo Akademi University, Department of Biochemistry and Pharmacy, FIN-20520 Turku, Finland 4Gene Therapy Unit, Kuopio University Hospital, Kuopio, Finland

    Article Title: Molecular cloning and partial functional characterization of Tsha3--a novel modulatory potassium channel alpha-subunit of trout CNS.
    Article Snippet: A novel Shaker-related potassium channel subunit termed Tsha3 that is widely expressed in the CNS of trout was PCR-cloned and sequenced: its deduced amino acid sequence showed an extended N-terminal domain with a high proportion of negatively charged residues and possessed highest similarity with KCNA10, a human epithelial potassium channel.. Upon heterologous expression in Sf21 cells, homomeric Tsha3 did not yield voltage-activated potassium channels but produced only ohmic currents that reversed at 15 mV.. After coexpression with Tsha1, a novel outward rectifier current was generated that differed from homomeric Tsha1 by its slower kinetics of activation, its partial current inactivation, and its partial blockade by 5 mM TEA as well as 1 AMDTX.

    Generated:

    Article Title: Molecular cloning and partial functional characterization of Tsha3--a novel modulatory potassium channel alpha-subunit of trout CNS.
    Article Snippet: A novel Shaker-related potassium channel subunit termed Tsha3 that is widely expressed in the CNS of trout was PCR-cloned and sequenced: its deduced amino acid sequence showed an extended N-terminal domain with a high proportion of negatively charged residues and possessed highest similarity with KCNA10, a human epithelial potassium channel.. Upon heterologous expression in Sf21 cells, homomeric Tsha3 did not yield voltage-activated potassium channels but produced only ohmic currents that reversed at 15 mV.. After coexpression with Tsha1, a novel outward rectifier current was generated that differed from homomeric Tsha1 by its slower kinetics of activation, its partial current inactivation, and its partial blockade by 5 mM TEA as well as 1 AMDTX.

    Article Title: Chimeric Composite Skin Substitutes for Delivery of Autologous Keratinocytes to Promote Tissue Regeneration
    Article Snippet: .. NIKS keratinocytes 7 stably expressing cytomegalovirus (CMV) promoter-driven GFP were generated by polycationic liposome-mediated transfection of pGreenLantern (Gibco-BRL, Rockville, MD) and pcDNA3neo (Invitrogen, Carlsbad, CA). .. NIKS cells cultivated on mitomycin C–treated Swiss mouse 3T3 fibroblast feeder layers 10 were transfected using GeneFector (VennNova, Miami, FL) in Dulbecco’s modified Eagle’s medium.

    Cloning:

    Article Title: Molecular cloning and partial functional characterization of Tsha3--a novel modulatory potassium channel alpha-subunit of trout CNS.
    Article Snippet: A novel Shaker-related potassium channel subunit termed Tsha3 that is widely expressed in the CNS of trout was PCR-cloned and sequenced: its deduced amino acid sequence showed an extended N-terminal domain with a high proportion of negatively charged residues and possessed highest similarity with KCNA10, a human epithelial potassium channel.. Upon heterologous expression in Sf21 cells, homomeric Tsha3 did not yield voltage-activated potassium channels but produced only ohmic currents that reversed at 15 mV.. After coexpression with Tsha1, a novel outward rectifier current was generated that differed from homomeric Tsha1 by its slower kinetics of activation, its partial current inactivation, and its partial blockade by 5 mM TEA as well as 1 AMDTX.

    Article Title: Sumoylation delays the ATF7 transcription factor subcellular localization and inhibits its transcriptional activity
    Article Snippet: .. The pATF-GFP was constructed by cloning the sequence encoding the N-terminal portion of ATF7 (amino acids 1–250) into an engineered Xho I site immediately 3′ to the start codon of GFP in pXRGG , a derivative of pGreenLantern (Life Technologies). ..

    Amplification:

    Article Title: Genome-wide RNAi screen reveals a specific sensitivity of IRES-containing RNA viruses to host translation inhibition
    Article Snippet: .. The gfp ORF was amplified by PCR from pGreenlantern (Invitrogen) and inserted between conserved VSV gene-start end sequences engineered at the leader-N gene junction. ..

    Polymerase Chain Reaction:

    Article Title: Genome-wide RNAi screen reveals a specific sensitivity of IRES-containing RNA viruses to host translation inhibition
    Article Snippet: .. The gfp ORF was amplified by PCR from pGreenlantern (Invitrogen) and inserted between conserved VSV gene-start end sequences engineered at the leader-N gene junction. ..

    Construct:

    Article Title: Sumoylation delays the ATF7 transcription factor subcellular localization and inhibits its transcriptional activity
    Article Snippet: .. The pATF-GFP was constructed by cloning the sequence encoding the N-terminal portion of ATF7 (amino acids 1–250) into an engineered Xho I site immediately 3′ to the start codon of GFP in pXRGG , a derivative of pGreenLantern (Life Technologies). ..

    Sequencing:

    Article Title: Sumoylation delays the ATF7 transcription factor subcellular localization and inhibits its transcriptional activity
    Article Snippet: .. The pATF-GFP was constructed by cloning the sequence encoding the N-terminal portion of ATF7 (amino acids 1–250) into an engineered Xho I site immediately 3′ to the start codon of GFP in pXRGG , a derivative of pGreenLantern (Life Technologies). ..

    Transfection:

    Article Title: Inositol 1,4,5-trisphosphate receptor movement is restricted by addition of elevated levels of O-linked sugar
    Article Snippet: GFP-polycystin-2-expressing MDCK cells were grown on glass bottom culture dishes (MatTek) in Dulbecco’s Modified Eagle Medium, supplemented with 10 % Fetal Bovine Serum, 10mM sodium pyruvate, and penicillin/streptomycin (Invitrogen). .. 2.2 Transfection Cells were transfected with pGreenLantern (GIBCO, BRL), pBFC013 or pBFC016 (generous gifts of T. Hughes) using Fugene 6 or Fugene HD (Roche Applied Science) reagent in Opti-MEM I (Invitrogen), according to the manufacturer’s instructions. ..

    Article Title: Chimeric Composite Skin Substitutes for Delivery of Autologous Keratinocytes to Promote Tissue Regeneration
    Article Snippet: .. NIKS keratinocytes 7 stably expressing cytomegalovirus (CMV) promoter-driven GFP were generated by polycationic liposome-mediated transfection of pGreenLantern (Gibco-BRL, Rockville, MD) and pcDNA3neo (Invitrogen, Carlsbad, CA). .. NIKS cells cultivated on mitomycin C–treated Swiss mouse 3T3 fibroblast feeder layers 10 were transfected using GeneFector (VennNova, Miami, FL) in Dulbecco’s modified Eagle’s medium.

    Stable Transfection:

    Article Title: Chimeric Composite Skin Substitutes for Delivery of Autologous Keratinocytes to Promote Tissue Regeneration
    Article Snippet: .. NIKS keratinocytes 7 stably expressing cytomegalovirus (CMV) promoter-driven GFP were generated by polycationic liposome-mediated transfection of pGreenLantern (Gibco-BRL, Rockville, MD) and pcDNA3neo (Invitrogen, Carlsbad, CA). .. NIKS cells cultivated on mitomycin C–treated Swiss mouse 3T3 fibroblast feeder layers 10 were transfected using GeneFector (VennNova, Miami, FL) in Dulbecco’s modified Eagle’s medium.

    Expressing:

    Article Title: Chimeric Composite Skin Substitutes for Delivery of Autologous Keratinocytes to Promote Tissue Regeneration
    Article Snippet: .. NIKS keratinocytes 7 stably expressing cytomegalovirus (CMV) promoter-driven GFP were generated by polycationic liposome-mediated transfection of pGreenLantern (Gibco-BRL, Rockville, MD) and pcDNA3neo (Invitrogen, Carlsbad, CA). .. NIKS cells cultivated on mitomycin C–treated Swiss mouse 3T3 fibroblast feeder layers 10 were transfected using GeneFector (VennNova, Miami, FL) in Dulbecco’s modified Eagle’s medium.



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    A. Representative micrographs of TH immunohistochemistry. Headings = Anterior-posterior coordinates of Paxinos and Watson Rat Atlas . 24WLUT = Untransfected rats with 24 weeks of 6-OHDA lesion. 12WL4WT = 12 weeks after lesion and 4 weeks after transfection, 12WL12WT = 12 weeks after lesion and 12 weeks after transfection. The scale bar of 300 μm is common for slices at different levels and of 50 μm for details. B. TH(+) neuron counting. C. Densitometry of TH(+) relative area. 4WT and 12 WT = 4 and 12 weeks after transfection. 24WL = untransfected rats with 24 weeks of lesion. 12WL = 12 weeks after lesion. The transfections of <t>pGreenLantern-1</t> (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion. All values are the mean ± SEM ( n = 3 independent rats for each experimental condition). One-way ANOVA and Newman-Keuls post-test. * P < 0.001, ** P < 0.0001. ns = no statistical significance, P > 0.05.
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    A. Representative micrographs of TH immunohistochemistry. Headings = Anterior-posterior coordinates of Paxinos and Watson Rat Atlas . 24WLUT = Untransfected rats with 24 weeks of 6-OHDA lesion. 12WL4WT = 12 weeks after lesion and 4 weeks after transfection, 12WL12WT = 12 weeks after lesion and 12 weeks after transfection. The scale bar of 300 μm is common for slices at different levels and of 50 μm for details. B. TH(+) neuron counting. C. Densitometry of TH(+) relative area. 4WT and 12 WT = 4 and 12 weeks after transfection. 24WL = untransfected rats with 24 weeks of lesion. 12WL = 12 weeks after lesion. The transfections of <t>pGreenLantern-1</t> (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion. All values are the mean ± SEM ( n = 3 independent rats for each experimental condition). One-way ANOVA and Newman-Keuls post-test. * P < 0.001, ** P < 0.0001. ns = no statistical significance, P > 0.05.
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    Thermo Fisher the modi®ed gfp-coding sequence was from vector pgreenlantern
    A. Representative micrographs of TH immunohistochemistry. Headings = Anterior-posterior coordinates of Paxinos and Watson Rat Atlas . 24WLUT = Untransfected rats with 24 weeks of 6-OHDA lesion. 12WL4WT = 12 weeks after lesion and 4 weeks after transfection, 12WL12WT = 12 weeks after lesion and 12 weeks after transfection. The scale bar of 300 μm is common for slices at different levels and of 50 μm for details. B. TH(+) neuron counting. C. Densitometry of TH(+) relative area. 4WT and 12 WT = 4 and 12 weeks after transfection. 24WL = untransfected rats with 24 weeks of lesion. 12WL = 12 weeks after lesion. The transfections of <t>pGreenLantern-1</t> (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion. All values are the mean ± SEM ( n = 3 independent rats for each experimental condition). One-way ANOVA and Newman-Keuls post-test. * P < 0.001, ** P < 0.0001. ns = no statistical significance, P > 0.05.
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    Image Search Results


    A. Representative micrographs of TH immunohistochemistry. Headings = Anterior-posterior coordinates of Paxinos and Watson Rat Atlas . 24WLUT = Untransfected rats with 24 weeks of 6-OHDA lesion. 12WL4WT = 12 weeks after lesion and 4 weeks after transfection, 12WL12WT = 12 weeks after lesion and 12 weeks after transfection. The scale bar of 300 μm is common for slices at different levels and of 50 μm for details. B. TH(+) neuron counting. C. Densitometry of TH(+) relative area. 4WT and 12 WT = 4 and 12 weeks after transfection. 24WL = untransfected rats with 24 weeks of lesion. 12WL = 12 weeks after lesion. The transfections of pGreenLantern-1 (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion. All values are the mean ± SEM ( n = 3 independent rats for each experimental condition). One-way ANOVA and Newman-Keuls post-test. * P < 0.001, ** P < 0.0001. ns = no statistical significance, P > 0.05.

    Journal: PLoS ONE

    Article Title: Neurturin overexpression in dopaminergic neurons induces presynaptic and postsynaptic structural changes in rats with chronic 6-hydroxydopamine lesion

    doi: 10.1371/journal.pone.0188239

    Figure Lengend Snippet: A. Representative micrographs of TH immunohistochemistry. Headings = Anterior-posterior coordinates of Paxinos and Watson Rat Atlas . 24WLUT = Untransfected rats with 24 weeks of 6-OHDA lesion. 12WL4WT = 12 weeks after lesion and 4 weeks after transfection, 12WL12WT = 12 weeks after lesion and 12 weeks after transfection. The scale bar of 300 μm is common for slices at different levels and of 50 μm for details. B. TH(+) neuron counting. C. Densitometry of TH(+) relative area. 4WT and 12 WT = 4 and 12 weeks after transfection. 24WL = untransfected rats with 24 weeks of lesion. 12WL = 12 weeks after lesion. The transfections of pGreenLantern-1 (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion. All values are the mean ± SEM ( n = 3 independent rats for each experimental condition). One-way ANOVA and Newman-Keuls post-test. * P < 0.001, ** P < 0.0001. ns = no statistical significance, P > 0.05.

    Article Snippet: Full NRTN sequence and correct Tag addition were confirmed by automatic sequencing (BigDye v3.1; Applied Biosystems; Foster City, CA, USA). pGreenLantern-1 (5,030 bp) coding for GFP under the control of CMV promoter (Gibco BRL; Grand Island, NY, USA) was used as a negative control.

    Techniques: Immunohistochemistry, Transfection

    A. Representative micrographs of TH immunohistochemistry. Headings = Anterior-posterior coordinates of Paxinos and Watson Rat Atlas . 24WLUT = Untransfected rats with 24 weeks of 6-OHDA lesion. 12WL4WT = 12 weeks after lesion and 4 weeks after transfection, 12WL12WT = 12 weeks after lesion and 12 weeks after transfection. The scale bar of 1 mm is for slices at different levels and of 100 μm for details. B. Densitometry of TH(+) relative area. 4WT and 12 WT = 4 and 12 weeks after transfection. 24WL = untransfected rats with 24 weeks of lesion. 12WL = 12 weeks after lesion. The transfections of pGreenLantern-1 (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion. One-way ANOVA and Newman-Keuls post-test. * P < 0.001, ** P < 0.0001. ns = no statistical significance, P > 0.05.

    Journal: PLoS ONE

    Article Title: Neurturin overexpression in dopaminergic neurons induces presynaptic and postsynaptic structural changes in rats with chronic 6-hydroxydopamine lesion

    doi: 10.1371/journal.pone.0188239

    Figure Lengend Snippet: A. Representative micrographs of TH immunohistochemistry. Headings = Anterior-posterior coordinates of Paxinos and Watson Rat Atlas . 24WLUT = Untransfected rats with 24 weeks of 6-OHDA lesion. 12WL4WT = 12 weeks after lesion and 4 weeks after transfection, 12WL12WT = 12 weeks after lesion and 12 weeks after transfection. The scale bar of 1 mm is for slices at different levels and of 100 μm for details. B. Densitometry of TH(+) relative area. 4WT and 12 WT = 4 and 12 weeks after transfection. 24WL = untransfected rats with 24 weeks of lesion. 12WL = 12 weeks after lesion. The transfections of pGreenLantern-1 (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion. One-way ANOVA and Newman-Keuls post-test. * P < 0.001, ** P < 0.0001. ns = no statistical significance, P > 0.05.

    Article Snippet: Full NRTN sequence and correct Tag addition were confirmed by automatic sequencing (BigDye v3.1; Applied Biosystems; Foster City, CA, USA). pGreenLantern-1 (5,030 bp) coding for GFP under the control of CMV promoter (Gibco BRL; Grand Island, NY, USA) was used as a negative control.

    Techniques: Immunohistochemistry, Transfection

    A. Representative micrographs of double immunofluorescence staining against NTSR1 and TH. 24WLUT = Untransfected rats with 24 weeks of 6-OHDA lesion. 12WL4WT = 12 weeks after lesion and 4 weeks after transfection, 12WL12WT = 12 weeks after lesion and 12 weeks after transfection. The scale is common for all micrographs. B. immunofluorescence area density (IFAD) for NTSR1 and TH was determined using ImageJ software v.1.46r (National Institutes of Health; Bethesda, MD). NRTN4WT and NRTN412WT = 4 and 12 weeks after transfection. UT = untransfected rats. The transfections of pGreenLantern-1 (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion. All values are the mean ± SEM ( n = 3 independent rats for each experimental condition). Two-way ANOVA and Bonferroni post-test. ns = no statistical significance.

    Journal: PLoS ONE

    Article Title: Neurturin overexpression in dopaminergic neurons induces presynaptic and postsynaptic structural changes in rats with chronic 6-hydroxydopamine lesion

    doi: 10.1371/journal.pone.0188239

    Figure Lengend Snippet: A. Representative micrographs of double immunofluorescence staining against NTSR1 and TH. 24WLUT = Untransfected rats with 24 weeks of 6-OHDA lesion. 12WL4WT = 12 weeks after lesion and 4 weeks after transfection, 12WL12WT = 12 weeks after lesion and 12 weeks after transfection. The scale is common for all micrographs. B. immunofluorescence area density (IFAD) for NTSR1 and TH was determined using ImageJ software v.1.46r (National Institutes of Health; Bethesda, MD). NRTN4WT and NRTN412WT = 4 and 12 weeks after transfection. UT = untransfected rats. The transfections of pGreenLantern-1 (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion. All values are the mean ± SEM ( n = 3 independent rats for each experimental condition). Two-way ANOVA and Bonferroni post-test. ns = no statistical significance.

    Article Snippet: Full NRTN sequence and correct Tag addition were confirmed by automatic sequencing (BigDye v3.1; Applied Biosystems; Foster City, CA, USA). pGreenLantern-1 (5,030 bp) coding for GFP under the control of CMV promoter (Gibco BRL; Grand Island, NY, USA) was used as a negative control.

    Techniques: Double Immunofluorescence Staining, Transfection, Immunofluorescence, Software

    A. Representative micrographs of double immunofluorescence staining against NTSR1 and TH. 24WLUT = Untransfected rats with 24 weeks of 6-OHDA lesion. 12WL4WT = 12 weeks after lesion and 4 weeks after transfection, 12WL12WT = 12 weeks after lesion and 12 weeks after transfection. The scale is common for all micrographs. B. immunofluorescence area density (IFAD) for NTSR1 and TH was determined using ImageJ software v.1.46r (National Institutes of Health; Bethesda, MD). NRTN4WT and NRTN412WT = 4 and 12 weeks after transfection. UT = untransfected rats. The transfections of pGreenLantern-1 (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion. All values are the mean ± SEM (n = 3 independent rats for each experimental condition). Two-way ANOVA and Bonferroni post-test. ns = no statistical significance.

    Journal: PLoS ONE

    Article Title: Neurturin overexpression in dopaminergic neurons induces presynaptic and postsynaptic structural changes in rats with chronic 6-hydroxydopamine lesion

    doi: 10.1371/journal.pone.0188239

    Figure Lengend Snippet: A. Representative micrographs of double immunofluorescence staining against NTSR1 and TH. 24WLUT = Untransfected rats with 24 weeks of 6-OHDA lesion. 12WL4WT = 12 weeks after lesion and 4 weeks after transfection, 12WL12WT = 12 weeks after lesion and 12 weeks after transfection. The scale is common for all micrographs. B. immunofluorescence area density (IFAD) for NTSR1 and TH was determined using ImageJ software v.1.46r (National Institutes of Health; Bethesda, MD). NRTN4WT and NRTN412WT = 4 and 12 weeks after transfection. UT = untransfected rats. The transfections of pGreenLantern-1 (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion. All values are the mean ± SEM (n = 3 independent rats for each experimental condition). Two-way ANOVA and Bonferroni post-test. ns = no statistical significance.

    Article Snippet: Full NRTN sequence and correct Tag addition were confirmed by automatic sequencing (BigDye v3.1; Applied Biosystems; Foster City, CA, USA). pGreenLantern-1 (5,030 bp) coding for GFP under the control of CMV promoter (Gibco BRL; Grand Island, NY, USA) was used as a negative control.

    Techniques: Double Immunofluorescence Staining, Transfection, Immunofluorescence, Software

    Representative micrographs of double immunofluorescence to TH and β-III-tubulin. 24WLUT = Untransfected rats with 24 weeks of 6-OHDA lesion. 12WL4WT = 12 weeks after lesion and 4 weeks after transfection, 12WL12WT = 12 weeks after lesion and 12 weeks after transfection. The transfections of pGreenLantern-1 (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion. The scale bar is common for all micrographs.

    Journal: PLoS ONE

    Article Title: Neurturin overexpression in dopaminergic neurons induces presynaptic and postsynaptic structural changes in rats with chronic 6-hydroxydopamine lesion

    doi: 10.1371/journal.pone.0188239

    Figure Lengend Snippet: Representative micrographs of double immunofluorescence to TH and β-III-tubulin. 24WLUT = Untransfected rats with 24 weeks of 6-OHDA lesion. 12WL4WT = 12 weeks after lesion and 4 weeks after transfection, 12WL12WT = 12 weeks after lesion and 12 weeks after transfection. The transfections of pGreenLantern-1 (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion. The scale bar is common for all micrographs.

    Article Snippet: Full NRTN sequence and correct Tag addition were confirmed by automatic sequencing (BigDye v3.1; Applied Biosystems; Foster City, CA, USA). pGreenLantern-1 (5,030 bp) coding for GFP under the control of CMV promoter (Gibco BRL; Grand Island, NY, USA) was used as a negative control.

    Techniques: Immunofluorescence, Transfection

    A . Sholl diagram for the morphological study of dendrites and dendritic spines. B . Representative micrograph of a medium spiny neuron (MSN) of a healthy striatum. The arrow shows the 50 μm segment (primary dendrite) where dendritic spines were analyzed. Upper calibration bar = 25 μm. The small panels show different types of spines (thin, mushroom, stubby and wide, from left to right) indicated by the arrow. Calibration bar = 5 μm. The transfections of pGreenLantern-1 (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion and the Sholl analysis was performed at the end of the study (12 weeks after transfection or 24 weeks after lesion). UT = untransfected rats with lesion. C. Sholl analysis of total number of intersections along dendritic trees in MSNs at all distances in 200-μm radius from the soma. D. Plot of mean spine density analyzed per 50-μm primary dendrite from cell body (proximal segment, upper arrow in B ) from six neurons per rat. All values represent the mean ± SEM ( n = 6 independent rats in each experimental condition). Statistical analysis ( C and D ) was performed using one-way ANOVA and Tukey post-hoc test.

    Journal: PLoS ONE

    Article Title: Neurturin overexpression in dopaminergic neurons induces presynaptic and postsynaptic structural changes in rats with chronic 6-hydroxydopamine lesion

    doi: 10.1371/journal.pone.0188239

    Figure Lengend Snippet: A . Sholl diagram for the morphological study of dendrites and dendritic spines. B . Representative micrograph of a medium spiny neuron (MSN) of a healthy striatum. The arrow shows the 50 μm segment (primary dendrite) where dendritic spines were analyzed. Upper calibration bar = 25 μm. The small panels show different types of spines (thin, mushroom, stubby and wide, from left to right) indicated by the arrow. Calibration bar = 5 μm. The transfections of pGreenLantern-1 (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion and the Sholl analysis was performed at the end of the study (12 weeks after transfection or 24 weeks after lesion). UT = untransfected rats with lesion. C. Sholl analysis of total number of intersections along dendritic trees in MSNs at all distances in 200-μm radius from the soma. D. Plot of mean spine density analyzed per 50-μm primary dendrite from cell body (proximal segment, upper arrow in B ) from six neurons per rat. All values represent the mean ± SEM ( n = 6 independent rats in each experimental condition). Statistical analysis ( C and D ) was performed using one-way ANOVA and Tukey post-hoc test.

    Article Snippet: Full NRTN sequence and correct Tag addition were confirmed by automatic sequencing (BigDye v3.1; Applied Biosystems; Foster City, CA, USA). pGreenLantern-1 (5,030 bp) coding for GFP under the control of CMV promoter (Gibco BRL; Grand Island, NY, USA) was used as a negative control.

    Techniques: Transfection

    Ipsilateral circling behavior ( A and C ) was activated by methamphetamine (8 mg/kg; i.p.), and contralateral circling behavior ( B and D ) was activated by apomorphine (0.5 mg/kg; i.p.) at weeks 0, 4 and 12 after transfection. The transfections of pGreenLantern-1 (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion (Week 0). All values represent the mean ± SEM ( n = 4 independent rats in each experimental condition). One-way ANOVA and Newman-Keuls post-test. * P <0.0005, ** P < 0.0001.

    Journal: PLoS ONE

    Article Title: Neurturin overexpression in dopaminergic neurons induces presynaptic and postsynaptic structural changes in rats with chronic 6-hydroxydopamine lesion

    doi: 10.1371/journal.pone.0188239

    Figure Lengend Snippet: Ipsilateral circling behavior ( A and C ) was activated by methamphetamine (8 mg/kg; i.p.), and contralateral circling behavior ( B and D ) was activated by apomorphine (0.5 mg/kg; i.p.) at weeks 0, 4 and 12 after transfection. The transfections of pGreenLantern-1 (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion (Week 0). All values represent the mean ± SEM ( n = 4 independent rats in each experimental condition). One-way ANOVA and Newman-Keuls post-test. * P <0.0005, ** P < 0.0001.

    Article Snippet: Full NRTN sequence and correct Tag addition were confirmed by automatic sequencing (BigDye v3.1; Applied Biosystems; Foster City, CA, USA). pGreenLantern-1 (5,030 bp) coding for GFP under the control of CMV promoter (Gibco BRL; Grand Island, NY, USA) was used as a negative control.

    Techniques: Transfection

    The transfections of pGreenLantern-1 (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion. UT = untransfected rats. The tests were performed at week 4 (12WL4WT) and week 12 (12WL12WT) after transfection. All values represent the mean ± SEM ( n = 4 independent rats in each experimental condition). * P < 0.001 when compared the values of the contralateral limb (Contra limb) with those of the ipsilateral limb (Ipsi limb), ** P < 0.001 when compared NRTN-His vs UT and § P < 0.001, NRTN-His vs GFP using two-way ANOVA. The post-hoc test was Newman-Keuls analysis.

    Journal: PLoS ONE

    Article Title: Neurturin overexpression in dopaminergic neurons induces presynaptic and postsynaptic structural changes in rats with chronic 6-hydroxydopamine lesion

    doi: 10.1371/journal.pone.0188239

    Figure Lengend Snippet: The transfections of pGreenLantern-1 (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion. UT = untransfected rats. The tests were performed at week 4 (12WL4WT) and week 12 (12WL12WT) after transfection. All values represent the mean ± SEM ( n = 4 independent rats in each experimental condition). * P < 0.001 when compared the values of the contralateral limb (Contra limb) with those of the ipsilateral limb (Ipsi limb), ** P < 0.001 when compared NRTN-His vs UT and § P < 0.001, NRTN-His vs GFP using two-way ANOVA. The post-hoc test was Newman-Keuls analysis.

    Article Snippet: Full NRTN sequence and correct Tag addition were confirmed by automatic sequencing (BigDye v3.1; Applied Biosystems; Foster City, CA, USA). pGreenLantern-1 (5,030 bp) coding for GFP under the control of CMV promoter (Gibco BRL; Grand Island, NY, USA) was used as a negative control.

    Techniques: Transfection