plasmids pgl3 basic (Addgene inc)
Addgene inc is a verified supplier 
93
Structured Review
Addgene inc
plasmids pgl3 basic
Plasmids Pgl3 Basic, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgl3-basic+plasmid/pRF%2B275Dux4+(Plasmid+%2321293)/pmc12666318-90-3-5
Average 93 stars, based on 29 article reviews
Plasmids Pgl3 Basic, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgl3-basic+plasmid/pRF%2B275Dux4+(Plasmid+%2321293)/pmc12666318-90-3-5
Average 93 stars, based on 29 article reviews
plasmids pgl3 basic - by Bioz Stars,
2026-10
93/100 stars
Images
Related Articles
Sequencing:Article Title: Paracrine stimulation of brown adipose tissue vascularization by adipocyte O-GlcNAc signaling. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Metabolomics RAW data This paper NMDR: ST004216; DIO: https://doi.org/ 10.21228/M82V85 Experimental models: Cell lines C3H/10T1/2 Procell Life Science & Technology CL-0325 3T3-L1 National Collection of Autjenticated Cell Cultures GNM25 HUVEC Procell Life Science & Technology N/A 293T Procell Life Science & Technology CL-0005 Experimental models: Organisms/strains Mouse: C57BL/6 mice Charles River Laboratories N/A Mouse: OGT-flox and Adipoq-Cre mice Jackson Laboratory N/A Mouse: Ucp1-CreER mice Shanghai Model Organisms Center NM-KI-190102 Oligonucleotides siRNA targeting sequence: OGT: GAUUAAGCCUGUUGAAGUC Peng et al.54 N/A siRNA targeting sequence: SP1: CCAGCAACAUGGGAAUUAUdTdT Lu et al.55 N/A siRNA targeting sequence: KCP#1: CTTCACCCTCCAAGAGAAT This paper N/A siRNA targeting sequence: KCP#2: CAACCTATGCACCTGCCTT This paper N/A Real-time qPCR primers This paper See Table S1 ChIP-PCR primers This paper See Table S2 Plasmid construction primers This paper See Tables S3 and S4 Recombinant DNA pGL3 Basic Vector Addgene Cat# Real-time Polymerase Chain Reaction:Article Title: Paracrine stimulation of brown adipose tissue vascularization by adipocyte O-GlcNAc signaling. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Metabolomics RAW data This paper NMDR: ST004216; DIO: https://doi.org/ 10.21228/M82V85 Experimental models: Cell lines C3H/10T1/2 Procell Life Science & Technology CL-0325 3T3-L1 National Collection of Autjenticated Cell Cultures GNM25 HUVEC Procell Life Science & Technology N/A 293T Procell Life Science & Technology CL-0005 Experimental models: Organisms/strains Mouse: C57BL/6 mice Charles River Laboratories N/A Mouse: OGT-flox and Adipoq-Cre mice Jackson Laboratory N/A Mouse: Ucp1-CreER mice Shanghai Model Organisms Center NM-KI-190102 Oligonucleotides siRNA targeting sequence: OGT: GAUUAAGCCUGUUGAAGUC Peng et al.54 N/A siRNA targeting sequence: SP1: CCAGCAACAUGGGAAUUAUdTdT Lu et al.55 N/A siRNA targeting sequence: KCP#1: CTTCACCCTCCAAGAGAAT This paper N/A siRNA targeting sequence: KCP#2: CAACCTATGCACCTGCCTT This paper N/A Real-time qPCR primers This paper See Table S1 ChIP-PCR primers This paper See Table S2 Plasmid construction primers This paper See Tables S3 and S4 Recombinant DNA pGL3 Basic Vector Addgene Cat# Chromatin Immunoprecipitation:Article Title: Paracrine stimulation of brown adipose tissue vascularization by adipocyte O-GlcNAc signaling. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Metabolomics RAW data This paper NMDR: ST004216; DIO: https://doi.org/ 10.21228/M82V85 Experimental models: Cell lines C3H/10T1/2 Procell Life Science & Technology CL-0325 3T3-L1 National Collection of Autjenticated Cell Cultures GNM25 HUVEC Procell Life Science & Technology N/A 293T Procell Life Science & Technology CL-0005 Experimental models: Organisms/strains Mouse: C57BL/6 mice Charles River Laboratories N/A Mouse: OGT-flox and Adipoq-Cre mice Jackson Laboratory N/A Mouse: Ucp1-CreER mice Shanghai Model Organisms Center NM-KI-190102 Oligonucleotides siRNA targeting sequence: OGT: GAUUAAGCCUGUUGAAGUC Peng et al.54 N/A siRNA targeting sequence: SP1: CCAGCAACAUGGGAAUUAUdTdT Lu et al.55 N/A siRNA targeting sequence: KCP#1: CTTCACCCTCCAAGAGAAT This paper N/A siRNA targeting sequence: KCP#2: CAACCTATGCACCTGCCTT This paper N/A Real-time qPCR primers This paper See Table S1 ChIP-PCR primers This paper See Table S2 Plasmid construction primers This paper See Tables S3 and S4 Recombinant DNA pGL3 Basic Vector Addgene Cat# Plasmid Preparation:Article Title: Paracrine stimulation of brown adipose tissue vascularization by adipocyte O-GlcNAc signaling. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Metabolomics RAW data This paper NMDR: ST004216; DIO: https://doi.org/ 10.21228/M82V85 Experimental models: Cell lines C3H/10T1/2 Procell Life Science & Technology CL-0325 3T3-L1 National Collection of Autjenticated Cell Cultures GNM25 HUVEC Procell Life Science & Technology N/A 293T Procell Life Science & Technology CL-0005 Experimental models: Organisms/strains Mouse: C57BL/6 mice Charles River Laboratories N/A Mouse: OGT-flox and Adipoq-Cre mice Jackson Laboratory N/A Mouse: Ucp1-CreER mice Shanghai Model Organisms Center NM-KI-190102 Oligonucleotides siRNA targeting sequence: OGT: GAUUAAGCCUGUUGAAGUC Peng et al.54 N/A siRNA targeting sequence: SP1: CCAGCAACAUGGGAAUUAUdTdT Lu et al.55 N/A siRNA targeting sequence: KCP#1: CTTCACCCTCCAAGAGAAT This paper N/A siRNA targeting sequence: KCP#2: CAACCTATGCACCTGCCTT This paper N/A Real-time qPCR primers This paper See Table S1 ChIP-PCR primers This paper See Table S2 Plasmid construction primers This paper See Tables S3 and S4 Recombinant DNA pGL3 Basic Vector Addgene Cat# Article Title: Bone Metastatic Progression of Prostate Cancer is Regulated by TRIM28-LDHA Mediated Metabolism Article Snippet: LDHA promoter luciferase reporter assays were conducted according to the manual of Dual-Luciferase Reporter Assay System from Promega. .. Briefly, C4–2B cells were seeded in a 24-well plate and co-transfected with the Renilla expression plasmid pRL-TK (RRID:Addgene_27163) and the reporter constructs for LDHA promoter in Article Title: UHRF1 restricts HCoV-229E infection through epigenetic silencing of the viral receptor APN Article Snippet: A Chemiluminescent EMSA kit (Beyotime #GS009) was used to detect interactions between the probes and nuclear proteins extracted from control and UHRF1 -knockout A549 cells using NE-PERTM Nuclear and Cytoplasmic Extraction Reagents (Thermo #78833). .. The APN promoter sequences (−153 to −1) were cloned into the Recombinant:Article Title: Paracrine stimulation of brown adipose tissue vascularization by adipocyte O-GlcNAc signaling. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Metabolomics RAW data This paper NMDR: ST004216; DIO: https://doi.org/ 10.21228/M82V85 Experimental models: Cell lines C3H/10T1/2 Procell Life Science & Technology CL-0325 3T3-L1 National Collection of Autjenticated Cell Cultures GNM25 HUVEC Procell Life Science & Technology N/A 293T Procell Life Science & Technology CL-0005 Experimental models: Organisms/strains Mouse: C57BL/6 mice Charles River Laboratories N/A Mouse: OGT-flox and Adipoq-Cre mice Jackson Laboratory N/A Mouse: Ucp1-CreER mice Shanghai Model Organisms Center NM-KI-190102 Oligonucleotides siRNA targeting sequence: OGT: GAUUAAGCCUGUUGAAGUC Peng et al.54 N/A siRNA targeting sequence: SP1: CCAGCAACAUGGGAAUUAUdTdT Lu et al.55 N/A siRNA targeting sequence: KCP#1: CTTCACCCTCCAAGAGAAT This paper N/A siRNA targeting sequence: KCP#2: CAACCTATGCACCTGCCTT This paper N/A Real-time qPCR primers This paper See Table S1 ChIP-PCR primers This paper See Table S2 Plasmid construction primers This paper See Tables S3 and S4 Recombinant DNA pGL3 Basic Vector Addgene Cat# Software:Article Title: Paracrine stimulation of brown adipose tissue vascularization by adipocyte O-GlcNAc signaling. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Metabolomics RAW data This paper NMDR: ST004216; DIO: https://doi.org/ 10.21228/M82V85 Experimental models: Cell lines C3H/10T1/2 Procell Life Science & Technology CL-0325 3T3-L1 National Collection of Autjenticated Cell Cultures GNM25 HUVEC Procell Life Science & Technology N/A 293T Procell Life Science & Technology CL-0005 Experimental models: Organisms/strains Mouse: C57BL/6 mice Charles River Laboratories N/A Mouse: OGT-flox and Adipoq-Cre mice Jackson Laboratory N/A Mouse: Ucp1-CreER mice Shanghai Model Organisms Center NM-KI-190102 Oligonucleotides siRNA targeting sequence: OGT: GAUUAAGCCUGUUGAAGUC Peng et al.54 N/A siRNA targeting sequence: SP1: CCAGCAACAUGGGAAUUAUdTdT Lu et al.55 N/A siRNA targeting sequence: KCP#1: CTTCACCCTCCAAGAGAAT This paper N/A siRNA targeting sequence: KCP#2: CAACCTATGCACCTGCCTT This paper N/A Real-time qPCR primers This paper See Table S1 ChIP-PCR primers This paper See Table S2 Plasmid construction primers This paper See Tables S3 and S4 Recombinant DNA pGL3 Basic Vector Addgene Cat# Imaging:Article Title: Paracrine stimulation of brown adipose tissue vascularization by adipocyte O-GlcNAc signaling. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Metabolomics RAW data This paper NMDR: ST004216; DIO: https://doi.org/ 10.21228/M82V85 Experimental models: Cell lines C3H/10T1/2 Procell Life Science & Technology CL-0325 3T3-L1 National Collection of Autjenticated Cell Cultures GNM25 HUVEC Procell Life Science & Technology N/A 293T Procell Life Science & Technology CL-0005 Experimental models: Organisms/strains Mouse: C57BL/6 mice Charles River Laboratories N/A Mouse: OGT-flox and Adipoq-Cre mice Jackson Laboratory N/A Mouse: Ucp1-CreER mice Shanghai Model Organisms Center NM-KI-190102 Oligonucleotides siRNA targeting sequence: OGT: GAUUAAGCCUGUUGAAGUC Peng et al.54 N/A siRNA targeting sequence: SP1: CCAGCAACAUGGGAAUUAUdTdT Lu et al.55 N/A siRNA targeting sequence: KCP#1: CTTCACCCTCCAAGAGAAT This paper N/A siRNA targeting sequence: KCP#2: CAACCTATGCACCTGCCTT This paper N/A Real-time qPCR primers This paper See Table S1 ChIP-PCR primers This paper See Table S2 Plasmid construction primers This paper See Tables S3 and S4 Recombinant DNA pGL3 Basic Vector Addgene Cat# Light Microscopy:Article Title: Paracrine stimulation of brown adipose tissue vascularization by adipocyte O-GlcNAc signaling. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Metabolomics RAW data This paper NMDR: ST004216; DIO: https://doi.org/ 10.21228/M82V85 Experimental models: Cell lines C3H/10T1/2 Procell Life Science & Technology CL-0325 3T3-L1 National Collection of Autjenticated Cell Cultures GNM25 HUVEC Procell Life Science & Technology N/A 293T Procell Life Science & Technology CL-0005 Experimental models: Organisms/strains Mouse: C57BL/6 mice Charles River Laboratories N/A Mouse: OGT-flox and Adipoq-Cre mice Jackson Laboratory N/A Mouse: Ucp1-CreER mice Shanghai Model Organisms Center NM-KI-190102 Oligonucleotides siRNA targeting sequence: OGT: GAUUAAGCCUGUUGAAGUC Peng et al.54 N/A siRNA targeting sequence: SP1: CCAGCAACAUGGGAAUUAUdTdT Lu et al.55 N/A siRNA targeting sequence: KCP#1: CTTCACCCTCCAAGAGAAT This paper N/A siRNA targeting sequence: KCP#2: CAACCTATGCACCTGCCTT This paper N/A Real-time qPCR primers This paper See Table S1 ChIP-PCR primers This paper See Table S2 Plasmid construction primers This paper See Tables S3 and S4 Recombinant DNA pGL3 Basic Vector Addgene Cat# Modification:Article Title: Paracrine stimulation of brown adipose tissue vascularization by adipocyte O-GlcNAc signaling. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Metabolomics RAW data This paper NMDR: ST004216; DIO: https://doi.org/ 10.21228/M82V85 Experimental models: Cell lines C3H/10T1/2 Procell Life Science & Technology CL-0325 3T3-L1 National Collection of Autjenticated Cell Cultures GNM25 HUVEC Procell Life Science & Technology N/A 293T Procell Life Science & Technology CL-0005 Experimental models: Organisms/strains Mouse: C57BL/6 mice Charles River Laboratories N/A Mouse: OGT-flox and Adipoq-Cre mice Jackson Laboratory N/A Mouse: Ucp1-CreER mice Shanghai Model Organisms Center NM-KI-190102 Oligonucleotides siRNA targeting sequence: OGT: GAUUAAGCCUGUUGAAGUC Peng et al.54 N/A siRNA targeting sequence: SP1: CCAGCAACAUGGGAAUUAUdTdT Lu et al.55 N/A siRNA targeting sequence: KCP#1: CTTCACCCTCCAAGAGAAT This paper N/A siRNA targeting sequence: KCP#2: CAACCTATGCACCTGCCTT This paper N/A Real-time qPCR primers This paper See Table S1 ChIP-PCR primers This paper See Table S2 Plasmid construction primers This paper See Tables S3 and S4 Recombinant DNA pGL3 Basic Vector Addgene Cat# Electroporation:Article Title: Amyotrophic Lateral Sclerosis-associated 3′ UTR enhancer embedded within CAV1 risk gene Article Snippet: .. Electroporation of SH-SY5Y cells with pGL3-Promoter (and derivatives, Promega #E1761), Derivative Assay:Article Title: Amyotrophic Lateral Sclerosis-associated 3′ UTR enhancer embedded within CAV1 risk gene Article Snippet: .. Electroporation of SH-SY5Y cells with pGL3-Promoter (and derivatives, Promega #E1761), Transfection:Article Title: Amyotrophic Lateral Sclerosis-associated 3′ UTR enhancer embedded within CAV1 risk gene Article Snippet: .. Electroporation of SH-SY5Y cells with pGL3-Promoter (and derivatives, Promega #E1761), Expressing:Article Title: Bone Metastatic Progression of Prostate Cancer is Regulated by TRIM28-LDHA Mediated Metabolism Article Snippet: LDHA promoter luciferase reporter assays were conducted according to the manual of Dual-Luciferase Reporter Assay System from Promega. .. Briefly, C4–2B cells were seeded in a 24-well plate and co-transfected with the Renilla expression plasmid pRL-TK (RRID:Addgene_27163) and the reporter constructs for LDHA promoter in Construct:Article Title: Bone Metastatic Progression of Prostate Cancer is Regulated by TRIM28-LDHA Mediated Metabolism Article Snippet: LDHA promoter luciferase reporter assays were conducted according to the manual of Dual-Luciferase Reporter Assay System from Promega. .. Briefly, C4–2B cells were seeded in a 24-well plate and co-transfected with the Renilla expression plasmid pRL-TK (RRID:Addgene_27163) and the reporter constructs for LDHA promoter in Clone Assay:Article Title: UHRF1 restricts HCoV-229E infection through epigenetic silencing of the viral receptor APN Article Snippet: A Chemiluminescent EMSA kit (Beyotime #GS009) was used to detect interactions between the probes and nuclear proteins extracted from control and UHRF1 -knockout A549 cells using NE-PERTM Nuclear and Cytoplasmic Extraction Reagents (Thermo #78833). .. The APN promoter sequences (−153 to −1) were cloned into the Luciferase:Article Title: tRF-21 LeuTAA Promotes Oxidative Stress by Altering Glutathione Metabolic Enzymes to Support Prostate Cancer Progression Article Snippet: Gene Ontology, Kyoto Encyclopedia of Genes and Genomes, and Reactome analyses and visualization were performed using OmicShare ( https://www.omicshare.com ) and SangerBox ( http://www.sangerbox.com/ ). .. The firefly luciferase–expressing Article Title: Activation of WDHD1 by DNA demethylases promotes MINPP1 transcription and glycolysis in lung adenocarcinoma progression Article Snippet: .. The MINPP1 promoter (chr10: 87504645–87504992) obtained from UCSC ( https://genome.ucsc.edu/index.html ) was amplified and inserted into the Amplification:Article Title: Activation of WDHD1 by DNA demethylases promotes MINPP1 transcription and glycolysis in lung adenocarcinoma progression Article Snippet: .. The MINPP1 promoter (chr10: 87504645–87504992) obtained from UCSC ( https://genome.ucsc.edu/index.html ) was amplified and inserted into the Control:Article Title: Activation of WDHD1 by DNA demethylases promotes MINPP1 transcription and glycolysis in lung adenocarcinoma progression Article Snippet: .. The MINPP1 promoter (chr10: 87504645–87504992) obtained from UCSC ( https://genome.ucsc.edu/index.html ) was amplified and inserted into the |
