pgl3 promega empty firefly luciferase vector rc203227 origene denr (Addgene inc)
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Pgl3 Promega Empty Firefly Luciferase Vector Rc203227 Origene Denr, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 71 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgl3+empty+vector/JDS246+(Plasmid+%2343861)/pm37862172-189-34-30
Average 94 stars, based on 71 article reviews
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Plasmid Preparation:Article Title: Characterization and inactivation of endogenous retroviruses in chinese hamster ovary cells Article Snippet: .. Plasmid Construction The mammalian Article Title: Cas9 variants and uses thereof Article Snippet: PCR was performed with 0.4 μL of 2 U/μL Phusion Hot Start Flex DNA polymerase (NEB) in 50 μL with 1×HF Buffer, 0.2 mM dNTP mix (0.2 mM dATP, 0.2 mM dCTP, 0.2 mM dGTP, 0.2 mM dTTP) (NEB), 0.5 μM of each primer and a program of: 98° C., 1 min; 35 cycles of [98° C. 15 s; 65° C., 15 s; 72° C., 30 s] unless otherwise noted. .. Construction of FokI-dCas9, Cas9 Nickase and gRNA Expression Plasmids The human codon-optimized Streptococcus pyogenes Cas9 nuclease with NLS and Article Title: CAS9 proteins including ligand-dependent inteins Article Snippet: .. A plasmid encoding the human codon-optimized Streptococcus pyogenes Cas9 nuclease with an NLS and Article Title: <i>Giardia's</i> domed ventral disc architecture is essential for attachment and contributes to epithelial barrier disruption Article Snippet: Giardia lamblia is a widespread anaerobic protistan parasite causing significant diarrheal disease worldwide.. Giardia trophozoites attach extracellularly to the host gastrointestinal epithelium using a unique microtubule organelle, the ventral disc.. The complex, dome-shaped disc is composed of microribbon-crossbridge (MR-CB) protein complexes scaffolded onto a spiral microtubule array. Article Title: Inhibitors of RNA-guided nuclease target binding and uses thereof Article Snippet: In the plasmid nucleofection method, cells were nucleofected either with 440 ng of SpCas9 (Addgene Plasmid #43861) or 400 ng of SpCas9 along with 40 ng of sgRNA (pFYF1320 eGFP Site #1, Addgene Plasmid #47511). .. In the plasmid nucleofection method, cells were nucleofected either with 440 ng of Expressing:Article Title: Characterization and inactivation of endogenous retroviruses in chinese hamster ovary cells Article Snippet: .. Plasmid Construction The mammalian Article Title: Cas9 variants and uses thereof Article Snippet: PCR was performed with 0.4 μL of 2 U/μL Phusion Hot Start Flex DNA polymerase (NEB) in 50 μL with 1×HF Buffer, 0.2 mM dNTP mix (0.2 mM dATP, 0.2 mM dCTP, 0.2 mM dGTP, 0.2 mM dTTP) (NEB), 0.5 μM of each primer and a program of: 98° C., 1 min; 35 cycles of [98° C. 15 s; 65° C., 15 s; 72° C., 30 s] unless otherwise noted. .. Construction of FokI-dCas9, Cas9 Nickase and gRNA Expression Plasmids The human codon-optimized Streptococcus pyogenes Cas9 nuclease with NLS and Article Title: CAS9 proteins including ligand-dependent inteins Article Snippet: .. A plasmid encoding the human codon-optimized Streptococcus pyogenes Cas9 nuclease with an NLS and Introduce:Article Title: Characterization and inactivation of endogenous retroviruses in chinese hamster ovary cells Article Snippet: .. Plasmid Construction The mammalian other:Article Title: Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants Article Snippet: Cytidine base editor (CBE) constructs were generated by subcloning the open Article Title: Inhibitors of RNA-guided nuclease target binding and uses thereof Article Snippet: NA Software and Algorithms GraphPad Prism GraphPad Prism Software NA Inc. Spotfire Tibco NA MetaXpress Molecular Device NA MATLAB MathWorks NA |

