pgl3 basic luciferase reporter plasmid (Addgene inc)
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Pgl3 Basic Luciferase Reporter Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 195 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgl3+basic/pGL3+(-BRE)+Luciferase+(Plasmid+%2345127)/pmc12757066-200-18-22
Average 95 stars, based on 195 article reviews
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Cell Culture:Article Title: Method for preparing Article Snippet: .. COS7 monkey kidney cells (ATCC) were cultured on a 24-well plate at 1.5×105 cells/well and then a Transfection:Article Title: Method for preparing Article Snippet: .. COS7 monkey kidney cells (ATCC) were cultured on a 24-well plate at 1.5×105 cells/well and then a Plasmid Preparation:Article Title: The natural L370F ERα variant confers endocrine resistance and sensitivity to ATRA in metastatic breast cancer cells Article Snippet: .. To generate the plasmid pGL2Basic_Neo_RARE-NLuc-PEST, the RARE cassette was KpnI and HindIII excised from the Article Title: TFEB coordinates autophagosome biogenesis and ribophagy during starvation via SQSTM1 Article Snippet: .. The promoter region of human SQSTM1 was amplified by PCR from the U2OS genome and cloned into the Article Title: The natural L370F ERα variant confers endocrine resistance and sensitivity to ATRA in metastatic breast cancer cells. Article Snippet: .. To generate the plasmid pGL2Basic_Neo_RARE-NLuc-PEST, the RARE cassette was KpnI and HindIII excised from the Article Title: Evidence for G-quadruplex-mediated transactivation by the immediate-early 2 protein of human cytomegalovirus Article Snippet: .. To produce the UL146 promoter-driven luciferase reporter plasmid (UL146p-Luc), the UL146 promoter region (−927 to +108) was PCR-amplified as an NheI/NcoI fragment and cloned into Article Title: METTL3 Methylation Induces Decay of Endogenous Retroelement Transcripts to Promote Tumor Immune Evasion Article Snippet: The genes of SMYD1-5, 177 SETD7, SETD8 were inserted into pcDNA3.1(+) vector (RRID:Addgene_52535). .. The 178 SETD1A and E2F4 promoter region, spanning nucleotides −2000 to +100 relative to 179 the transcription start site (TSS), was PCR-amplified and ligated into the Article Title: Ebselen Suppresses Breast Cancer Tumorigenesis by Inhibiting YTHDF1-Mediated c-Fos Expression. Article Snippet: To design the translation reporter construct, the putative m6A modification site of FOS (chr4:5278828–75282230) was PCR-amplified from MCF7 cDNA and cloned into the 3′-UTR of RLuc at the XhoI site of the psiCHECK3 vector (a gift from Anthony Leung; Addgene plasmid #136010). .. The Amplification:Article Title: TFEB coordinates autophagosome biogenesis and ribophagy during starvation via SQSTM1 Article Snippet: .. The promoter region of human SQSTM1 was amplified by PCR from the U2OS genome and cloned into the Polymerase Chain Reaction:Article Title: TFEB coordinates autophagosome biogenesis and ribophagy during starvation via SQSTM1 Article Snippet: .. The promoter region of human SQSTM1 was amplified by PCR from the U2OS genome and cloned into the Article Title: Evidence for G-quadruplex-mediated transactivation by the immediate-early 2 protein of human cytomegalovirus Article Snippet: .. To produce the UL146 promoter-driven luciferase reporter plasmid (UL146p-Luc), the UL146 promoter region (−927 to +108) was PCR-amplified as an NheI/NcoI fragment and cloned into Article Title: METTL3 Methylation Induces Decay of Endogenous Retroelement Transcripts to Promote Tumor Immune Evasion Article Snippet: The genes of SMYD1-5, 177 SETD7, SETD8 were inserted into pcDNA3.1(+) vector (RRID:Addgene_52535). .. The 178 SETD1A and E2F4 promoter region, spanning nucleotides −2000 to +100 relative to 179 the transcription start site (TSS), was PCR-amplified and ligated into the Clone Assay:Article Title: TFEB coordinates autophagosome biogenesis and ribophagy during starvation via SQSTM1 Article Snippet: .. The promoter region of human SQSTM1 was amplified by PCR from the U2OS genome and cloned into the Article Title: Evidence for G-quadruplex-mediated transactivation by the immediate-early 2 protein of human cytomegalovirus Article Snippet: .. To produce the UL146 promoter-driven luciferase reporter plasmid (UL146p-Luc), the UL146 promoter region (−927 to +108) was PCR-amplified as an NheI/NcoI fragment and cloned into Luciferase:Article Title: TFEB coordinates autophagosome biogenesis and ribophagy during starvation via SQSTM1 Article Snippet: .. The promoter region of human SQSTM1 was amplified by PCR from the U2OS genome and cloned into the Article Title: tRF-21 LeuTAA Promotes Oxidative Stress by Altering Glutathione Metabolic Enzymes to Support Prostate Cancer Progression Article Snippet: Gene Ontology, Kyoto Encyclopedia of Genes and Genomes, and Reactome analyses and visualization were performed using OmicShare ( https://www.omicshare.com ) and SangerBox ( http://www.sangerbox.com/ ). .. The firefly luciferase–expressing plasmids pGL3-basic (RRID:Addgene_212936), Article Title: Evidence for G-quadruplex-mediated transactivation by the immediate-early 2 protein of human cytomegalovirus Article Snippet: .. To produce the UL146 promoter-driven luciferase reporter plasmid (UL146p-Luc), the UL146 promoter region (−927 to +108) was PCR-amplified as an NheI/NcoI fragment and cloned into Article Title: METTL3 Methylation Induces Decay of Endogenous Retroelement Transcripts to Promote Tumor Immune Evasion Article Snippet: The genes of SMYD1-5, 177 SETD7, SETD8 were inserted into pcDNA3.1(+) vector (RRID:Addgene_52535). .. The 178 SETD1A and E2F4 promoter region, spanning nucleotides −2000 to +100 relative to 179 the transcription start site (TSS), was PCR-amplified and ligated into the |
