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pgl3 basic luciferase reporter plasmid  (Addgene inc)


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    Structured Review

    Addgene inc pgl3 basic luciferase reporter plasmid
    Pgl3 Basic Luciferase Reporter Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 195 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgl3+basic/pGL3+(-BRE)+Luciferase+(Plasmid+%2345127)/pmc12757066-200-18-22
    Average 95 stars, based on 195 article reviews
    pgl3 basic luciferase reporter plasmid - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Method for preparing
    Article Snippet: .. COS7 monkey kidney cells (ATCC) were cultured on a 24-well plate at 1.5×105 cells/well and then a pGL3-PGC-1α-Luc plasm id (Addgene, Cambridge, MA, USA) was transfected into the cells using Lipofector (Aptabio, Yongin, Korea). ..

    Transfection:

    Article Title: Method for preparing
    Article Snippet: .. COS7 monkey kidney cells (ATCC) were cultured on a 24-well plate at 1.5×105 cells/well and then a pGL3-PGC-1α-Luc plasm id (Addgene, Cambridge, MA, USA) was transfected into the cells using Lipofector (Aptabio, Yongin, Korea). ..

    Plasmid Preparation:

    Article Title: The natural L370F ERα variant confers endocrine resistance and sensitivity to ATRA in metastatic breast cancer cells
    Article Snippet: .. To generate the plasmid pGL2Basic_Neo_RARE-NLuc-PEST, the RARE cassette was KpnI and HindIII excised from the pGL3-RARE-luciferase [ ] purchased from Addgene (USA) and ligated into the corresponding restriction sites in the pGL2Basic_Neo_NLuc-PEST [ , ]. ..

    Article Title: TFEB coordinates autophagosome biogenesis and ribophagy during starvation via SQSTM1
    Article Snippet: .. The promoter region of human SQSTM1 was amplified by PCR from the U2OS genome and cloned into the pGL3-basic luciferase reporter plasmid (Addgene). ..

    Article Title: The natural L370F ERα variant confers endocrine resistance and sensitivity to ATRA in metastatic breast cancer cells.
    Article Snippet: .. To generate the plasmid pGL2Basic_Neo_RARE-NLuc-PEST, the RARE cassette was KpnI and HindIII excised from the pGL3-RAREluciferase [25] purchased from Addgene (USA) and ligated into the corresponding restriction sites in the pGL2Basic_Neo_NLuc-PEST [22, 23]. ..

    Article Title: Evidence for G-quadruplex-mediated transactivation by the immediate-early 2 protein of human cytomegalovirus
    Article Snippet: .. To produce the UL146 promoter-driven luciferase reporter plasmid (UL146p-Luc), the UL146 promoter region (−927 to +108) was PCR-amplified as an NheI/NcoI fragment and cloned into pGL3-basic (Addgene). ..

    Article Title: METTL3 Methylation Induces Decay of Endogenous Retroelement Transcripts to Promote Tumor Immune Evasion
    Article Snippet: The genes of SMYD1-5, 177 SETD7, SETD8 were inserted into pcDNA3.1(+) vector (RRID:Addgene_52535). .. The 178 SETD1A and E2F4 promoter region, spanning nucleotides −2000 to +100 relative to 179 the transcription start site (TSS), was PCR-amplified and ligated into the pGL3-Basic 180 luciferase reporter plasmid (RRID:Addgene_212936). ..

    Article Title: Ebselen Suppresses Breast Cancer Tumorigenesis by Inhibiting YTHDF1-Mediated c-Fos Expression.
    Article Snippet: To design the translation reporter construct, the putative m6A modification site of FOS (chr4:5278828–75282230) was PCR-amplified from MCF7 cDNA and cloned into the 3′-UTR of RLuc at the XhoI site of the psiCHECK3 vector (a gift from Anthony Leung; Addgene plasmid #136010). .. The pGL3-FOS-Luc plasmid was a gift from Ron Prywes (Addgene plasmid #11983). ..

    Amplification:

    Article Title: TFEB coordinates autophagosome biogenesis and ribophagy during starvation via SQSTM1
    Article Snippet: .. The promoter region of human SQSTM1 was amplified by PCR from the U2OS genome and cloned into the pGL3-basic luciferase reporter plasmid (Addgene). ..

    Polymerase Chain Reaction:

    Article Title: TFEB coordinates autophagosome biogenesis and ribophagy during starvation via SQSTM1
    Article Snippet: .. The promoter region of human SQSTM1 was amplified by PCR from the U2OS genome and cloned into the pGL3-basic luciferase reporter plasmid (Addgene). ..

    Article Title: Evidence for G-quadruplex-mediated transactivation by the immediate-early 2 protein of human cytomegalovirus
    Article Snippet: .. To produce the UL146 promoter-driven luciferase reporter plasmid (UL146p-Luc), the UL146 promoter region (−927 to +108) was PCR-amplified as an NheI/NcoI fragment and cloned into pGL3-basic (Addgene). ..

    Article Title: METTL3 Methylation Induces Decay of Endogenous Retroelement Transcripts to Promote Tumor Immune Evasion
    Article Snippet: The genes of SMYD1-5, 177 SETD7, SETD8 were inserted into pcDNA3.1(+) vector (RRID:Addgene_52535). .. The 178 SETD1A and E2F4 promoter region, spanning nucleotides −2000 to +100 relative to 179 the transcription start site (TSS), was PCR-amplified and ligated into the pGL3-Basic 180 luciferase reporter plasmid (RRID:Addgene_212936). ..

    Clone Assay:

    Article Title: TFEB coordinates autophagosome biogenesis and ribophagy during starvation via SQSTM1
    Article Snippet: .. The promoter region of human SQSTM1 was amplified by PCR from the U2OS genome and cloned into the pGL3-basic luciferase reporter plasmid (Addgene). ..

    Article Title: Evidence for G-quadruplex-mediated transactivation by the immediate-early 2 protein of human cytomegalovirus
    Article Snippet: .. To produce the UL146 promoter-driven luciferase reporter plasmid (UL146p-Luc), the UL146 promoter region (−927 to +108) was PCR-amplified as an NheI/NcoI fragment and cloned into pGL3-basic (Addgene). ..

    Luciferase:

    Article Title: TFEB coordinates autophagosome biogenesis and ribophagy during starvation via SQSTM1
    Article Snippet: .. The promoter region of human SQSTM1 was amplified by PCR from the U2OS genome and cloned into the pGL3-basic luciferase reporter plasmid (Addgene). ..

    Article Title: tRF-21 LeuTAA Promotes Oxidative Stress by Altering Glutathione Metabolic Enzymes to Support Prostate Cancer Progression
    Article Snippet: Gene Ontology, Kyoto Encyclopedia of Genes and Genomes, and Reactome analyses and visualization were performed using OmicShare ( https://www.omicshare.com ) and SangerBox ( http://www.sangerbox.com/ ). .. The firefly luciferase–expressing plasmids pGL3-basic (RRID:Addgene_212936), pGL3-promoter (RRID:Addgene_172006), and Renilla pRL-CMV (RRID:Addgene_41046) were purchased from Promega (TM033, E2261). ..

    Article Title: Evidence for G-quadruplex-mediated transactivation by the immediate-early 2 protein of human cytomegalovirus
    Article Snippet: .. To produce the UL146 promoter-driven luciferase reporter plasmid (UL146p-Luc), the UL146 promoter region (−927 to +108) was PCR-amplified as an NheI/NcoI fragment and cloned into pGL3-basic (Addgene). ..

    Article Title: METTL3 Methylation Induces Decay of Endogenous Retroelement Transcripts to Promote Tumor Immune Evasion
    Article Snippet: The genes of SMYD1-5, 177 SETD7, SETD8 were inserted into pcDNA3.1(+) vector (RRID:Addgene_52535). .. The 178 SETD1A and E2F4 promoter region, spanning nucleotides −2000 to +100 relative to 179 the transcription start site (TSS), was PCR-amplified and ligated into the pGL3-Basic 180 luciferase reporter plasmid (RRID:Addgene_212936). ..



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    Addgene inc pgl3 basic luciferase reporter
    Estrogen promotes HNRNPAB expression via SP1. (A) Immunoblot assessment of HNRNPAB in 2 human normal breast epithelial cell lines and 11 human BC cell lines. (B) CPTAC database analyzed the relative HNRNPAB and ERα protein level in 105 BC patients. P value was determined by Pearson correlation analysis. (C) The relative expression of TFF1 and HNRNPAB mRNAs after stimulated with 10 nM E 2 in estrogen-deprived MCF-7 cells for 48 h analyzed by qRT-PCR. (D) Immunoblot assessment of HNRNPAB expression in MCF-7 with or without 10 nM E 2 in estrogen-deprived MCF-7 cells for 72 h, or in short-term oestrogen deprivation (STED) MCF-7 cells for 7 days and parental cells. (E) The schematic of HNRNPAB promoter region sequence selected from the transcription start site of -2 kb~+100 bp. (F) MCF-7 cells were transfected with <t>pGL3</t> basic reporter vector containing HNRNPAB promoter, and pRL-TK reporter control vector containing luciferase activity, as well as control siRNA or different siRNA sequences of JUN , FOS , FOXA1 and SP1 . The relative fluorescence activity was measured. (G) CPTAC database analyzed the relative HNRNPAB and SP1 protein level in 68 ER+ BC patients. P value was determined by Pearson correlation analysis. (H) 293T cells were transfected with control or SP1 vector, and pRL-TK reporter control vector containing luciferase activity, as well as pGL3 basic reporter vector containing different regions of HNRNPAB promoter. The relative fluorescence activity was measured. (I) The schematic of three predicted conserved SP1 binding sites on the upstream -425 bp~+100 bp regions of the human HNRNPAB gene. Red boxes represent the predicted SP1 binding sites. (J) The DNA regions enriched by SP1 in ChIP assay were verified by PCR. (K) 293T cells were transfected with control or SP1 vector, and pRL-TK reporter control vector containing luciferase activity, as well as pGL3 basic reporter vector or containing “b” or “b” mutant regions. The relative fluorescence activity was measured. (L) The relative expression of SP1 and HNRNPAB mRNAs in MCF-7 cells bearing control or SP1 shRNAs analyzed by qRT-PCR. (M) Immunoblot assessment of SP1 and HNRNPAB expression in MCF-7 cells with or without SP1 shRNAs in the presence or absence of 10 nM E 2 for 48 h. Data was shown as mean ± S.D. from three independent experiments. Unpaired two-tailed Student's t test (C, H, K) and one-way ANOVA followed by Tukey's multiple comparisons test (F, L). ns, P >0.05; ***, P <0.001; ****, P <0.0001.
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    Image Search Results


    Estrogen promotes HNRNPAB expression via SP1. (A) Immunoblot assessment of HNRNPAB in 2 human normal breast epithelial cell lines and 11 human BC cell lines. (B) CPTAC database analyzed the relative HNRNPAB and ERα protein level in 105 BC patients. P value was determined by Pearson correlation analysis. (C) The relative expression of TFF1 and HNRNPAB mRNAs after stimulated with 10 nM E 2 in estrogen-deprived MCF-7 cells for 48 h analyzed by qRT-PCR. (D) Immunoblot assessment of HNRNPAB expression in MCF-7 with or without 10 nM E 2 in estrogen-deprived MCF-7 cells for 72 h, or in short-term oestrogen deprivation (STED) MCF-7 cells for 7 days and parental cells. (E) The schematic of HNRNPAB promoter region sequence selected from the transcription start site of -2 kb~+100 bp. (F) MCF-7 cells were transfected with pGL3 basic reporter vector containing HNRNPAB promoter, and pRL-TK reporter control vector containing luciferase activity, as well as control siRNA or different siRNA sequences of JUN , FOS , FOXA1 and SP1 . The relative fluorescence activity was measured. (G) CPTAC database analyzed the relative HNRNPAB and SP1 protein level in 68 ER+ BC patients. P value was determined by Pearson correlation analysis. (H) 293T cells were transfected with control or SP1 vector, and pRL-TK reporter control vector containing luciferase activity, as well as pGL3 basic reporter vector containing different regions of HNRNPAB promoter. The relative fluorescence activity was measured. (I) The schematic of three predicted conserved SP1 binding sites on the upstream -425 bp~+100 bp regions of the human HNRNPAB gene. Red boxes represent the predicted SP1 binding sites. (J) The DNA regions enriched by SP1 in ChIP assay were verified by PCR. (K) 293T cells were transfected with control or SP1 vector, and pRL-TK reporter control vector containing luciferase activity, as well as pGL3 basic reporter vector or containing “b” or “b” mutant regions. The relative fluorescence activity was measured. (L) The relative expression of SP1 and HNRNPAB mRNAs in MCF-7 cells bearing control or SP1 shRNAs analyzed by qRT-PCR. (M) Immunoblot assessment of SP1 and HNRNPAB expression in MCF-7 cells with or without SP1 shRNAs in the presence or absence of 10 nM E 2 for 48 h. Data was shown as mean ± S.D. from three independent experiments. Unpaired two-tailed Student's t test (C, H, K) and one-way ANOVA followed by Tukey's multiple comparisons test (F, L). ns, P >0.05; ***, P <0.001; ****, P <0.0001.

    Journal: International Journal of Biological Sciences

    Article Title: The Interaction of CircESR1 and HNRNPAB Regulates Cell Cycle Transition of Breast Cancer Cell

    doi: 10.7150/ijbs.126014

    Figure Lengend Snippet: Estrogen promotes HNRNPAB expression via SP1. (A) Immunoblot assessment of HNRNPAB in 2 human normal breast epithelial cell lines and 11 human BC cell lines. (B) CPTAC database analyzed the relative HNRNPAB and ERα protein level in 105 BC patients. P value was determined by Pearson correlation analysis. (C) The relative expression of TFF1 and HNRNPAB mRNAs after stimulated with 10 nM E 2 in estrogen-deprived MCF-7 cells for 48 h analyzed by qRT-PCR. (D) Immunoblot assessment of HNRNPAB expression in MCF-7 with or without 10 nM E 2 in estrogen-deprived MCF-7 cells for 72 h, or in short-term oestrogen deprivation (STED) MCF-7 cells for 7 days and parental cells. (E) The schematic of HNRNPAB promoter region sequence selected from the transcription start site of -2 kb~+100 bp. (F) MCF-7 cells were transfected with pGL3 basic reporter vector containing HNRNPAB promoter, and pRL-TK reporter control vector containing luciferase activity, as well as control siRNA or different siRNA sequences of JUN , FOS , FOXA1 and SP1 . The relative fluorescence activity was measured. (G) CPTAC database analyzed the relative HNRNPAB and SP1 protein level in 68 ER+ BC patients. P value was determined by Pearson correlation analysis. (H) 293T cells were transfected with control or SP1 vector, and pRL-TK reporter control vector containing luciferase activity, as well as pGL3 basic reporter vector containing different regions of HNRNPAB promoter. The relative fluorescence activity was measured. (I) The schematic of three predicted conserved SP1 binding sites on the upstream -425 bp~+100 bp regions of the human HNRNPAB gene. Red boxes represent the predicted SP1 binding sites. (J) The DNA regions enriched by SP1 in ChIP assay were verified by PCR. (K) 293T cells were transfected with control or SP1 vector, and pRL-TK reporter control vector containing luciferase activity, as well as pGL3 basic reporter vector or containing “b” or “b” mutant regions. The relative fluorescence activity was measured. (L) The relative expression of SP1 and HNRNPAB mRNAs in MCF-7 cells bearing control or SP1 shRNAs analyzed by qRT-PCR. (M) Immunoblot assessment of SP1 and HNRNPAB expression in MCF-7 cells with or without SP1 shRNAs in the presence or absence of 10 nM E 2 for 48 h. Data was shown as mean ± S.D. from three independent experiments. Unpaired two-tailed Student's t test (C, H, K) and one-way ANOVA followed by Tukey's multiple comparisons test (F, L). ns, P >0.05; ***, P <0.001; ****, P <0.0001.

    Article Snippet: For transcription factor mediated HNRNPAB expression, 0.2 μg pGL3 Basic luciferase reporter (RRID: Addgene_48743), 50 nmol JUN or FOS or FOXA1 or SP1 siRNAs and 0.02 μg pRL-TK plasmid (RRID: Addgene_11313) were transfected into cells using Lipofectamine 2000 (Invitrogen, Carlsbad, USA).

    Techniques: Expressing, Western Blot, Quantitative RT-PCR, Sequencing, Transfection, Plasmid Preparation, Control, Luciferase, Activity Assay, Fluorescence, Binding Assay, Mutagenesis, Two Tailed Test