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pgl2 basic vector  (OriGene)


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    Structured Review

    OriGene pgl2 basic vector
    (A, above) Western blot showing PC4, H3 and acetylated H3 levels in MCF7 cells transfected with scrambled or PC4 siRNA followed by treatment with or without TSA (500ng/ml) for 16 h revealed by PC4, H3 and specific H3 acetylated antibodies to H3 Lys residues (K9, K14, K18, K23, K27 and K36). (A, below) Western blot showing PC4 and H3.3 levels in MCF7 cells treated with scrambled or PC4 siRNA followed by treatment with or without TSA. β-actin is shown as a loading control. Results are representative of three independent experiments. (B) Effect of endogenous PC4 knock-down by siRNA on LHR promoter activity. Cells transfected with scrambled (control) or PC4 siRNA followed by transfection of LHR promoter construct or <t>PGL2</t> basic vector and later treated with or without TSA prior to measurement of promoter activity. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.001). (C) ChIP analysis illustrates relative enrichment of H3K9-Ac onto the LHR promoter of cells transfected with scrambled or PC4 siRNA cultured in the presence or absence of TSA. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.05). (D) H3.3 enrichment at the LHR promoter assessed in cells treated with and without TSA following PC4 knockdown. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.05). Representative western blot showing knock-down of PC4 protein in MCF7 cells following PC4 siRNA treatment. Data represent mean ± SE of two independent experiments performed in triplicates.
    Pgl2 Basic Vector, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgl2+basic+vector/pmc06668928-78-32-42?v=OriGene
    Average 92 stars, based on 1 article reviews
    pgl2 basic vector - by Bioz Stars, 2026-08
    92/100 stars

    Images

    1) Product Images from "Interaction of Positive Coactivator 4 with Histone 3.3 Protein is Essential for Transcriptional Activation of the Luteinizing Hormone Receptor Gene"

    Article Title: Interaction of Positive Coactivator 4 with Histone 3.3 Protein is Essential for Transcriptional Activation of the Luteinizing Hormone Receptor Gene

    Journal: Biochimica et biophysica acta. Gene regulatory mechanisms

    doi: 10.1016/j.bbagrm.2018.09.002

    (A, above) Western blot showing PC4, H3 and acetylated H3 levels in MCF7 cells transfected with scrambled or PC4 siRNA followed by treatment with or without TSA (500ng/ml) for 16 h revealed by PC4, H3 and specific H3 acetylated antibodies to H3 Lys residues (K9, K14, K18, K23, K27 and K36). (A, below) Western blot showing PC4 and H3.3 levels in MCF7 cells treated with scrambled or PC4 siRNA followed by treatment with or without TSA. β-actin is shown as a loading control. Results are representative of three independent experiments. (B) Effect of endogenous PC4 knock-down by siRNA on LHR promoter activity. Cells transfected with scrambled (control) or PC4 siRNA followed by transfection of LHR promoter construct or PGL2 basic vector and later treated with or without TSA prior to measurement of promoter activity. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.001). (C) ChIP analysis illustrates relative enrichment of H3K9-Ac onto the LHR promoter of cells transfected with scrambled or PC4 siRNA cultured in the presence or absence of TSA. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.05). (D) H3.3 enrichment at the LHR promoter assessed in cells treated with and without TSA following PC4 knockdown. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.05). Representative western blot showing knock-down of PC4 protein in MCF7 cells following PC4 siRNA treatment. Data represent mean ± SE of two independent experiments performed in triplicates.
    Figure Legend Snippet: (A, above) Western blot showing PC4, H3 and acetylated H3 levels in MCF7 cells transfected with scrambled or PC4 siRNA followed by treatment with or without TSA (500ng/ml) for 16 h revealed by PC4, H3 and specific H3 acetylated antibodies to H3 Lys residues (K9, K14, K18, K23, K27 and K36). (A, below) Western blot showing PC4 and H3.3 levels in MCF7 cells treated with scrambled or PC4 siRNA followed by treatment with or without TSA. β-actin is shown as a loading control. Results are representative of three independent experiments. (B) Effect of endogenous PC4 knock-down by siRNA on LHR promoter activity. Cells transfected with scrambled (control) or PC4 siRNA followed by transfection of LHR promoter construct or PGL2 basic vector and later treated with or without TSA prior to measurement of promoter activity. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.001). (C) ChIP analysis illustrates relative enrichment of H3K9-Ac onto the LHR promoter of cells transfected with scrambled or PC4 siRNA cultured in the presence or absence of TSA. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.05). (D) H3.3 enrichment at the LHR promoter assessed in cells treated with and without TSA following PC4 knockdown. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.05). Representative western blot showing knock-down of PC4 protein in MCF7 cells following PC4 siRNA treatment. Data represent mean ± SE of two independent experiments performed in triplicates.

    Techniques Used: Western Blot, Transfection, Activity Assay, Construct, Plasmid Preparation, Cell Culture



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