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pgl3 control vector  (Addgene inc)


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    Addgene inc pgl3 control vector
    Pgl3 Control Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgl+3+basic+vector/pGL3+Basic+Control+Vector+(Plasmid+%23137707)/pmc12172475-208-7-30
    Average 93 stars, based on 51 article reviews
    pgl3 control vector - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Binding Assay:

    Article Title: Bmal1 Regulates Vascular Calcification via Non-Canonical Circadian Pathway
    Article Snippet: The aortic expression of Runx2 and SMA in arteries from the STZ-treated Bmal1 flox/flox and Bmal1 Δ/Δ mice were determined by immunofluorescent staining of consecutive 7-μm aortic sections using specific primary antibodies (Runx2, MBL Cat# D130–3 and SMA, Sigma Cat# A5228). .. To assess a direct regulation of Bmal1 on Runx2 transcription, a 1,974-bp mouse Runx2 promoter (mm10: chr17:44,735,972–44,737,945) containing putative Bmal1 binding sites was insert into the pGL3-Basic vector (Addgene) to generate the wildtype mRunx2 promoter reporter construct (Bmal1 BS Mut −). .. Using this construct, the CACGTG Bmal1 binding site (chr17:44,737,386–44,737,391) within the mRunx2 promoter was deleted using the QuikChange Lightning Multi-Site-Directed Mutagenesis Kit (Agilent Technologies) to generate mutant mRunx2 promoter reporter construct (Bmal1 BS Mut +).

    Article Title: Targeted protein O-GlcNAc reveals transcriptional functions for O-GlcNAc.
    Article Snippet: Technology Targeted protein O-GlcNAc reveals transcriptional functions for O-GlcNAc

    Plasmid Preparation:

    Article Title: Bmal1 Regulates Vascular Calcification via Non-Canonical Circadian Pathway
    Article Snippet: The aortic expression of Runx2 and SMA in arteries from the STZ-treated Bmal1 flox/flox and Bmal1 Δ/Δ mice were determined by immunofluorescent staining of consecutive 7-μm aortic sections using specific primary antibodies (Runx2, MBL Cat# D130–3 and SMA, Sigma Cat# A5228). .. To assess a direct regulation of Bmal1 on Runx2 transcription, a 1,974-bp mouse Runx2 promoter (mm10: chr17:44,735,972–44,737,945) containing putative Bmal1 binding sites was insert into the pGL3-Basic vector (Addgene) to generate the wildtype mRunx2 promoter reporter construct (Bmal1 BS Mut −). .. Using this construct, the CACGTG Bmal1 binding site (chr17:44,737,386–44,737,391) within the mRunx2 promoter was deleted using the QuikChange Lightning Multi-Site-Directed Mutagenesis Kit (Agilent Technologies) to generate mutant mRunx2 promoter reporter construct (Bmal1 BS Mut +).

    Article Title: The NSP6-L260F substitution in SARS-CoV-2 BQ.1.1 and XBB.1.16 lineages compensates for the reduced viral polymerase activity caused by mutations in NSP13 and NSP14
    Article Snippet: .. The BACs encoding the replicative cDNA and pGL3-control vector were transfected into HEK293T cells using TransIT-LT1 (TaKaRa) according to the manufacturer’s instructions. pGL3 Control Vector, a gift from Debrya Groskreutz (Addgene plasmid #212937; http://n2t.net/addgene:212937 ; RRID: Addgene_212937), expressed firefly luciferase and served as an internal control. .. After incubation for 72 h at 37°C, the transfected cells were lysed with Passive Lysis buffer (Promega).

    Article Title: Quercetin suppresses endometrial cancer stem cells via ERα-mediated inhibition of STAT3 signaling
    Article Snippet: .. pGL3-human Oct4 PE-SV40-luc was purchase from Addgene; the vector pGL3 bought from Pomega was used as a control. .. The protein concentrations were ascertained through the Bradford protein assay (Bio-Rad Laboratories, Milano, Italy).

    Article Title: WNT3 promotes chemoresistance to oxaliplatin in oral squamous cell carcinoma via regulating ABCG2 expression
    Article Snippet: .. To assess Wnt/β-catenin signaling activity, cells were co-transfected with either WNT3 or control Vector plasmids, along with the TopFlash/FOPFlash plasmid and the Renilla luciferase internal control plasmid (Addgene, USA). ..

    Article Title: Supporting Information for Extrachromosomal Telomere DNA Derived from Excessive Strand Displacements
    Article Snippet: The siRNAs were transfected into the cells at a final concentration of 20 nM, along with 2 l of Lipofectamine 3000 (Thermo scientific, L3000001) according to the manufacturer's protocol. .. Lentiviruses were generated by transfecting shRNA vectors targeting human POLD3 (6), STN1 (7), ATRX(8), or the control vector, or sgRNA vectors targeting human PML exon 3 (sense: CACCGGcggtaccagcgcgactacg, antisense: AAACcgtagtcgcgctggtaccgCC) inserted in pLentiV2 vector (Addgene, 52961) along with pMD2.G and psPAX into 293T cells. .. Lentiviruses were generated by transfecting shRNA vectors targeting human POLD3 (6), STN1 (7), ATRX(8), or the control vector, or sgRNA vectors targeting human PML exon 3 (sense: CACCGGcggtaccagcgcgactacg, antisense: AAACcgtagtcgcgctggtaccgCC) inserted in pLentiV2 vector (Addgene, 52961) along with pMD2.G and psPAX into 293T cells.

    Construct:

    Article Title: Bmal1 Regulates Vascular Calcification via Non-Canonical Circadian Pathway
    Article Snippet: The aortic expression of Runx2 and SMA in arteries from the STZ-treated Bmal1 flox/flox and Bmal1 Δ/Δ mice were determined by immunofluorescent staining of consecutive 7-μm aortic sections using specific primary antibodies (Runx2, MBL Cat# D130–3 and SMA, Sigma Cat# A5228). .. To assess a direct regulation of Bmal1 on Runx2 transcription, a 1,974-bp mouse Runx2 promoter (mm10: chr17:44,735,972–44,737,945) containing putative Bmal1 binding sites was insert into the pGL3-Basic vector (Addgene) to generate the wildtype mRunx2 promoter reporter construct (Bmal1 BS Mut −). .. Using this construct, the CACGTG Bmal1 binding site (chr17:44,737,386–44,737,391) within the mRunx2 promoter was deleted using the QuikChange Lightning Multi-Site-Directed Mutagenesis Kit (Agilent Technologies) to generate mutant mRunx2 promoter reporter construct (Bmal1 BS Mut +).

    Article Title: Targeted protein O-GlcNAc reveals transcriptional functions for O-GlcNAc.
    Article Snippet: Technology Targeted protein O-GlcNAc reveals transcriptional functions for O-GlcNAc

    Article Title: β-catenin: A crucial transcriptional activator of KSHV latency genes and small molecule target in primary effusion lymphoma.
    Article Snippet: Latency-associated nuclear antigen (LANA)-induced β-catenin accumulation is one of the prime events in the Kaposi sarcoma-associated herpesvirus (KSHV)-associated primary effusion lymphoma (PEL).. LANA interacts with GSK3β, which in turn increases β-catenin pool in this lymphoma.. The intrinsic molecular mechanism and the effects of β-catenin accumulation in this lymphoma is still unclear. β-catenin acts as a major transcription factor that regulates a variety of proliferative signaling pathways and promotes oncogenesis.

    Clone Assay:

    Article Title: Targeted protein O-GlcNAc reveals transcriptional functions for O-GlcNAc.
    Article Snippet: Technology Targeted protein O-GlcNAc reveals transcriptional functions for O-GlcNAc

    Transfection:

    Article Title: The NSP6-L260F substitution in SARS-CoV-2 BQ.1.1 and XBB.1.16 lineages compensates for the reduced viral polymerase activity caused by mutations in NSP13 and NSP14
    Article Snippet: .. The BACs encoding the replicative cDNA and pGL3-control vector were transfected into HEK293T cells using TransIT-LT1 (TaKaRa) according to the manufacturer’s instructions. pGL3 Control Vector, a gift from Debrya Groskreutz (Addgene plasmid #212937; http://n2t.net/addgene:212937 ; RRID: Addgene_212937), expressed firefly luciferase and served as an internal control. .. After incubation for 72 h at 37°C, the transfected cells were lysed with Passive Lysis buffer (Promega).

    Control:

    Article Title: The NSP6-L260F substitution in SARS-CoV-2 BQ.1.1 and XBB.1.16 lineages compensates for the reduced viral polymerase activity caused by mutations in NSP13 and NSP14
    Article Snippet: .. The BACs encoding the replicative cDNA and pGL3-control vector were transfected into HEK293T cells using TransIT-LT1 (TaKaRa) according to the manufacturer’s instructions. pGL3 Control Vector, a gift from Debrya Groskreutz (Addgene plasmid #212937; http://n2t.net/addgene:212937 ; RRID: Addgene_212937), expressed firefly luciferase and served as an internal control. .. After incubation for 72 h at 37°C, the transfected cells were lysed with Passive Lysis buffer (Promega).

    Article Title: β-catenin: A crucial transcriptional activator of KSHV latency genes and small molecule target in primary effusion lymphoma.
    Article Snippet: Latency-associated nuclear antigen (LANA)-induced β-catenin accumulation is one of the prime events in the Kaposi sarcoma-associated herpesvirus (KSHV)-associated primary effusion lymphoma (PEL).. LANA interacts with GSK3β, which in turn increases β-catenin pool in this lymphoma.. The intrinsic molecular mechanism and the effects of β-catenin accumulation in this lymphoma is still unclear. β-catenin acts as a major transcription factor that regulates a variety of proliferative signaling pathways and promotes oncogenesis.

    Article Title: Quercetin suppresses endometrial cancer stem cells via ERα-mediated inhibition of STAT3 signaling
    Article Snippet: .. pGL3-human Oct4 PE-SV40-luc was purchase from Addgene; the vector pGL3 bought from Pomega was used as a control. .. The protein concentrations were ascertained through the Bradford protein assay (Bio-Rad Laboratories, Milano, Italy).

    Article Title: WNT3 promotes chemoresistance to oxaliplatin in oral squamous cell carcinoma via regulating ABCG2 expression
    Article Snippet: .. To assess Wnt/β-catenin signaling activity, cells were co-transfected with either WNT3 or control Vector plasmids, along with the TopFlash/FOPFlash plasmid and the Renilla luciferase internal control plasmid (Addgene, USA). ..

    Article Title: Supporting Information for Extrachromosomal Telomere DNA Derived from Excessive Strand Displacements
    Article Snippet: The siRNAs were transfected into the cells at a final concentration of 20 nM, along with 2 l of Lipofectamine 3000 (Thermo scientific, L3000001) according to the manufacturer's protocol. .. Lentiviruses were generated by transfecting shRNA vectors targeting human POLD3 (6), STN1 (7), ATRX(8), or the control vector, or sgRNA vectors targeting human PML exon 3 (sense: CACCGGcggtaccagcgcgactacg, antisense: AAACcgtagtcgcgctggtaccgCC) inserted in pLentiV2 vector (Addgene, 52961) along with pMD2.G and psPAX into 293T cells. .. Lentiviruses were generated by transfecting shRNA vectors targeting human POLD3 (6), STN1 (7), ATRX(8), or the control vector, or sgRNA vectors targeting human PML exon 3 (sense: CACCGGcggtaccagcgcgactacg, antisense: AAACcgtagtcgcgctggtaccgCC) inserted in pLentiV2 vector (Addgene, 52961) along with pMD2.G and psPAX into 293T cells.

    Luciferase:

    Article Title: The NSP6-L260F substitution in SARS-CoV-2 BQ.1.1 and XBB.1.16 lineages compensates for the reduced viral polymerase activity caused by mutations in NSP13 and NSP14
    Article Snippet: .. The BACs encoding the replicative cDNA and pGL3-control vector were transfected into HEK293T cells using TransIT-LT1 (TaKaRa) according to the manufacturer’s instructions. pGL3 Control Vector, a gift from Debrya Groskreutz (Addgene plasmid #212937; http://n2t.net/addgene:212937 ; RRID: Addgene_212937), expressed firefly luciferase and served as an internal control. .. After incubation for 72 h at 37°C, the transfected cells were lysed with Passive Lysis buffer (Promega).

    Article Title: WNT3 promotes chemoresistance to oxaliplatin in oral squamous cell carcinoma via regulating ABCG2 expression
    Article Snippet: .. To assess Wnt/β-catenin signaling activity, cells were co-transfected with either WNT3 or control Vector plasmids, along with the TopFlash/FOPFlash plasmid and the Renilla luciferase internal control plasmid (Addgene, USA). ..

    other:

    Article Title: E2F1 suppresses Epstein-Barr virus lytic reactivation through cellular and viral transcriptional networks
    Article Snippet: pGL3 Basic Vector , Gift from Debrya Groskreutz , RRID: Addgene_212936.

    Activity Assay:

    Article Title: WNT3 promotes chemoresistance to oxaliplatin in oral squamous cell carcinoma via regulating ABCG2 expression
    Article Snippet: .. To assess Wnt/β-catenin signaling activity, cells were co-transfected with either WNT3 or control Vector plasmids, along with the TopFlash/FOPFlash plasmid and the Renilla luciferase internal control plasmid (Addgene, USA). ..

    Generated:

    Article Title: Supporting Information for Extrachromosomal Telomere DNA Derived from Excessive Strand Displacements
    Article Snippet: The siRNAs were transfected into the cells at a final concentration of 20 nM, along with 2 l of Lipofectamine 3000 (Thermo scientific, L3000001) according to the manufacturer's protocol. .. Lentiviruses were generated by transfecting shRNA vectors targeting human POLD3 (6), STN1 (7), ATRX(8), or the control vector, or sgRNA vectors targeting human PML exon 3 (sense: CACCGGcggtaccagcgcgactacg, antisense: AAACcgtagtcgcgctggtaccgCC) inserted in pLentiV2 vector (Addgene, 52961) along with pMD2.G and psPAX into 293T cells. .. Lentiviruses were generated by transfecting shRNA vectors targeting human POLD3 (6), STN1 (7), ATRX(8), or the control vector, or sgRNA vectors targeting human PML exon 3 (sense: CACCGGcggtaccagcgcgactacg, antisense: AAACcgtagtcgcgctggtaccgCC) inserted in pLentiV2 vector (Addgene, 52961) along with pMD2.G and psPAX into 293T cells.

    shRNA:

    Article Title: Supporting Information for Extrachromosomal Telomere DNA Derived from Excessive Strand Displacements
    Article Snippet: The siRNAs were transfected into the cells at a final concentration of 20 nM, along with 2 l of Lipofectamine 3000 (Thermo scientific, L3000001) according to the manufacturer's protocol. .. Lentiviruses were generated by transfecting shRNA vectors targeting human POLD3 (6), STN1 (7), ATRX(8), or the control vector, or sgRNA vectors targeting human PML exon 3 (sense: CACCGGcggtaccagcgcgactacg, antisense: AAACcgtagtcgcgctggtaccgCC) inserted in pLentiV2 vector (Addgene, 52961) along with pMD2.G and psPAX into 293T cells. .. Lentiviruses were generated by transfecting shRNA vectors targeting human POLD3 (6), STN1 (7), ATRX(8), or the control vector, or sgRNA vectors targeting human PML exon 3 (sense: CACCGGcggtaccagcgcgactacg, antisense: AAACcgtagtcgcgctggtaccgCC) inserted in pLentiV2 vector (Addgene, 52961) along with pMD2.G and psPAX into 293T cells.



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    Promega pgl-3 luciferase reporter basic vector
    The (−2436/+149) region of the PAK3 promoter, containing several putative cJun/AP- binding sites, was cloned into the <t>pGL</t> <t>3</t> -Basic reporter vector. A: Luciferase promoter reporter assays performed in control cells, Rat1a-GFP, and Rat1a-J4 cells transiently transfected with empty vector, pGL 3 -Basic, and PAK3 promoter construct containing vector, pGL 3 -Basic-pPAK3 (−2436/+149). Cells were grown in the absence and presence of doxycycline. B: Luciferase promoter reporter assays performed in the parental rat fibroblast cell line, Rat1a, transfected with either pCMV-cJun or empty pCMV vector with the PAK3 promoter construct containing vector, pGL 3 -Basic-pPAK3 (−2436/+149). A plasmid containing four AP-1 binding sites, 4 X AP-1-Luc, was used as a control showing cJun activation. C: Luciferase reporter promoter assays for deletion constructs of the PAK3 promoter region (−2436/+149), (−2329/+149), (−684/+149) and (−179/+149) as well as a (−179/+149) with a mutated cJun/AP-1 binding site at position (+52/+60), in the presence and absence of doxycycline-induced cJun/AP-1 expression. D: Luciferase reporter assays, using the (−179/+149) PAK3 promoter construct, showing the effect of transient cJun inhibition on PAK3 promoter activity in the absence and presence of doxycycline induced cJun/AP-1 over-expression. Results show the mean±S.E. of experiments performed in triplicate and repeated at least three times. (*p≤0.05 and **p≤0.01).
    Pgl 3 Luciferase Reporter Basic Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgl+3+basic+vector/pgl3+basic/ppr0123714-41-34-38
    Average 90 stars, based on 1 article reviews
    pgl-3 luciferase reporter basic vector - by Bioz Stars, 2026-10
    90/100 stars
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    pgl-3 basic recombinant vector containing p53-re1 trim8 gene  (Promega)
    90
    Promega pgl-3 basic recombinant vector containing p53-re1 trim8 gene
    The (−2436/+149) region of the PAK3 promoter, containing several putative cJun/AP- binding sites, was cloned into the <t>pGL</t> <t>3</t> -Basic reporter vector. A: Luciferase promoter reporter assays performed in control cells, Rat1a-GFP, and Rat1a-J4 cells transiently transfected with empty vector, pGL 3 -Basic, and PAK3 promoter construct containing vector, pGL 3 -Basic-pPAK3 (−2436/+149). Cells were grown in the absence and presence of doxycycline. B: Luciferase promoter reporter assays performed in the parental rat fibroblast cell line, Rat1a, transfected with either pCMV-cJun or empty pCMV vector with the PAK3 promoter construct containing vector, pGL 3 -Basic-pPAK3 (−2436/+149). A plasmid containing four AP-1 binding sites, 4 X AP-1-Luc, was used as a control showing cJun activation. C: Luciferase reporter promoter assays for deletion constructs of the PAK3 promoter region (−2436/+149), (−2329/+149), (−684/+149) and (−179/+149) as well as a (−179/+149) with a mutated cJun/AP-1 binding site at position (+52/+60), in the presence and absence of doxycycline-induced cJun/AP-1 expression. D: Luciferase reporter assays, using the (−179/+149) PAK3 promoter construct, showing the effect of transient cJun inhibition on PAK3 promoter activity in the absence and presence of doxycycline induced cJun/AP-1 over-expression. Results show the mean±S.E. of experiments performed in triplicate and repeated at least three times. (*p≤0.05 and **p≤0.01).
    Pgl 3 Basic Recombinant Vector Containing P53 Re1 Trim8 Gene, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgl+3+basic+vector/pgl3+basic/pmc06856647-78-19-54
    Average 90 stars, based on 1 article reviews
    pgl-3 basic recombinant vector containing p53-re1 trim8 gene - by Bioz Stars, 2026-10
    90/100 stars
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    The (−2436/+149) region of the PAK3 promoter, containing several putative cJun/AP- binding sites, was cloned into the pGL 3 -Basic reporter vector. A: Luciferase promoter reporter assays performed in control cells, Rat1a-GFP, and Rat1a-J4 cells transiently transfected with empty vector, pGL 3 -Basic, and PAK3 promoter construct containing vector, pGL 3 -Basic-pPAK3 (−2436/+149). Cells were grown in the absence and presence of doxycycline. B: Luciferase promoter reporter assays performed in the parental rat fibroblast cell line, Rat1a, transfected with either pCMV-cJun or empty pCMV vector with the PAK3 promoter construct containing vector, pGL 3 -Basic-pPAK3 (−2436/+149). A plasmid containing four AP-1 binding sites, 4 X AP-1-Luc, was used as a control showing cJun activation. C: Luciferase reporter promoter assays for deletion constructs of the PAK3 promoter region (−2436/+149), (−2329/+149), (−684/+149) and (−179/+149) as well as a (−179/+149) with a mutated cJun/AP-1 binding site at position (+52/+60), in the presence and absence of doxycycline-induced cJun/AP-1 expression. D: Luciferase reporter assays, using the (−179/+149) PAK3 promoter construct, showing the effect of transient cJun inhibition on PAK3 promoter activity in the absence and presence of doxycycline induced cJun/AP-1 over-expression. Results show the mean±S.E. of experiments performed in triplicate and repeated at least three times. (*p≤0.05 and **p≤0.01).

    Journal: PLoS ONE

    Article Title: p21-Activated Kinase 3 (PAK3) Is an AP-1 Regulated Gene Contributing to Actin Organisation and Migration of Transformed Fibroblasts

    doi: 10.1371/journal.pone.0066892

    Figure Lengend Snippet: The (−2436/+149) region of the PAK3 promoter, containing several putative cJun/AP- binding sites, was cloned into the pGL 3 -Basic reporter vector. A: Luciferase promoter reporter assays performed in control cells, Rat1a-GFP, and Rat1a-J4 cells transiently transfected with empty vector, pGL 3 -Basic, and PAK3 promoter construct containing vector, pGL 3 -Basic-pPAK3 (−2436/+149). Cells were grown in the absence and presence of doxycycline. B: Luciferase promoter reporter assays performed in the parental rat fibroblast cell line, Rat1a, transfected with either pCMV-cJun or empty pCMV vector with the PAK3 promoter construct containing vector, pGL 3 -Basic-pPAK3 (−2436/+149). A plasmid containing four AP-1 binding sites, 4 X AP-1-Luc, was used as a control showing cJun activation. C: Luciferase reporter promoter assays for deletion constructs of the PAK3 promoter region (−2436/+149), (−2329/+149), (−684/+149) and (−179/+149) as well as a (−179/+149) with a mutated cJun/AP-1 binding site at position (+52/+60), in the presence and absence of doxycycline-induced cJun/AP-1 expression. D: Luciferase reporter assays, using the (−179/+149) PAK3 promoter construct, showing the effect of transient cJun inhibition on PAK3 promoter activity in the absence and presence of doxycycline induced cJun/AP-1 over-expression. Results show the mean±S.E. of experiments performed in triplicate and repeated at least three times. (*p≤0.05 and **p≤0.01).

    Article Snippet: PCR using high fidelity Expand Plus DNA Polymerase (Roche) was performed on rat genomic DNA, the products were sub-cloned into the pGEM-T Easy vector (Promega) and excised for cloning into the luciferase reported vector, pGL 3 -Basic (Promega) using Mlu1 and Xho1 restriction enzymes.

    Techniques: Binding Assay, Clone Assay, Plasmid Preparation, Luciferase, Transfection, Construct, Activation Assay, Expressing, Inhibition, Activity Assay, Over Expression