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Promega pgemex-2 e. coli expression vector
Pgemex 2 E. Coli Expression Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgemex-2+expression+vector/pgem+t+easy/pmc10693968-46-10-15
Average 90 stars, based on 1 article reviews
pgemex-2 e. coli expression vector - by Bioz Stars, 2026-09
90/100 stars

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other:

Article Title: MEG3 Enhances Survival of Developing Human Neurons with CLCN4 -Linked Autophagy Impairment
Article Snippet: PCR products were subcloned into pGEM-T easy (Promega, A1360) and total 10 clones were sequenced.

Article Title: VapA/Scs2 sustains polarized growth in Aspergillus nidulans by maintaining AP-2-mediated apical endocytosis
Article Snippet: Gene cassettes were generated by sequential cloning of the relevant fragments in the pGEM-T plasmid (Promega), which served as template to PCR-amplify the relevant linear cassettes.

Article Title: Tc BDF6 deficiency compromises intracellular amastigote development and infectivity in Trypanosoma cruzi
Article Snippet: A pGEM-T plasmid (Promega®) was used as a heterologous internal standard (IS) and all samples were mixed before DNA purification with 1 pg of the IS.

Article Title: HLA binding vaccine moieties and uses thereof
Article Snippet: The PCR products for the light chain and heavy chain were ligated into individual pGEM®-T Easy vectors (Promega, Madison, WI, USA).

Article Title: Supporting Information for Invasive mussels fashion silk-like byssus via mechanical processing of massive horizontally acquired coiled coils
Article Snippet: These fragments were cloned and sequenced using standard TA-cloning methods (pGEM-TA cloning system Promega #A1360).

Article Title: Synergistic DNA and RNA binding of the Hox transcription factor Ultrabithorax coordinates splicing and shapes in vivo homeotic functions.
Article Snippet: In vitro transcription with Cy3-UTP labelling For in vitro transcription, we employed selected fragments of alternatively spliced exons that were cloned into the pGEM®T Easy (Promega) Vector (listed in [ 21 ]).

Article Title: Supporting Information for FAN1 removes triplet repeat extrusions via a PCNA and RFC- dependent mechanism
Article Snippet: Plasmid name* Sense strand sequence Cloning site(s) pGB1mr77 5ʹTCGACCTAGCAGCTGCTGATCTCGAGTCTAGAAATTCG pGBmr78 5ʹTCGACCTAGCAGATCTCGAGTCTAGAAATTCG pGB1mr77 4xLacO 5ʹGCTAATTGTGAGCGGATAACAATTGTTAGGGAGGAATTGTGAGCGGATAACAATTTGGAG TTGATAATTGTGAGCGGATAACAATTGGCTTCAACGTAATTGTGAGCGGATAACAATTGCTCTTCC SapI pGB1mr78 4xLacO 5ʹGCTAATTGTGAGCGGATAACAATTGTTAGGGAGGAATTGTGAGCGGATAACAATTTGGAG TTGATAATTGTGAGCGGATAACAATTGGCTTCAACGTAATTGTGAGCGGATAACAATTGCTCTTCC SapI Entries 1 and 2 are phagemid pGEM-7Zf(-) (Promega) derivatives as described previously (9).

Purification:

Article Title: Allele editing and applications thereof
Article Snippet: .. 90 ng of the digested and purified 2 kb PGR amplicons were ligated for 1 h at 22° C. with 50 or 100 ng pGEM3Z plasmid which had been BamHI/Sall digested and purified (Promega), respectively. .. Transformation was performed by mixing 10 pl of the ligation reaction with 50 pl ice-cold chemically competent JM109 bacteria (purchased from Promega or made using the RbCI protocol http://openwetware.org/wiki/RbCLcompetent cell).

Plasmid Preparation:

Article Title: Allele editing and applications thereof
Article Snippet: .. 90 ng of the digested and purified 2 kb PGR amplicons were ligated for 1 h at 22° C. with 50 or 100 ng pGEM3Z plasmid which had been BamHI/Sall digested and purified (Promega), respectively. .. Transformation was performed by mixing 10 pl of the ligation reaction with 50 pl ice-cold chemically competent JM109 bacteria (purchased from Promega or made using the RbCI protocol http://openwetware.org/wiki/RbCLcompetent cell).



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