nbp1 04676 (Novus Biologicals)
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nbp1 04676
Nbp1 04676, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 96/100, based on 277 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgc1/PGC1+alpha+Antibody+-+BSA+Free/pmc13068818-9-2-3
Average 96 stars, based on 277 article reviews
Nbp1 04676, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 96/100, based on 277 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgc1/PGC1+alpha+Antibody+-+BSA+Free/pmc13068818-9-2-3
Average 96 stars, based on 277 article reviews
nbp1 04676 - by Bioz Stars,
2026-11
96/100 stars
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Cell-Signaling:Article Title: Strength training attenuates hepatic inflammation and potentiates mitochondrial adaptations in IL-10-deficient mice. Article Snippet: .. The following antibodies were used: microtubule-associated proteins 1 A/1B light chain 3B (LC3; #3868), total STAT3 (#30835S), phosphorylated STAT3 (p-STAT3, Tyr705; #4113S), Kelch-like ECH-associated protein 1 (Keap1; #8047), nuclear factor erythroid 2–related factor 2 (NRF2; #12721), and xanthine dehydrogenase/oxidase (XOR; #53370) from Cell Signaling Technology, MA, USA; Article Title: Peroxisome Proliferator-Activated Receptor α Deficiency Induces Vascular Pathologies through Endothelial Senescence in Diabetic Retinopathy Article Snippet: Chemiluminescent signals were developed by incubation with Pierce ECL Western Blotting Substrate (Thermo Fisher Scientific), quantified by densitometry using ImageJ version 1.54g, and normalized by β-actin levels. .. The primary antibodies included antibodies against PPARα (Thermo Fisher Scientific; catalog number MA1822; 1:1000), translocase of outer mitochondrial membrane 20 (TOMM20; Abcam, Waltham, MA; catalog number ab186735; 1:1000), Pyrolysis Gas Chromatography:Article Title: Strength training attenuates hepatic inflammation and potentiates mitochondrial adaptations in IL-10-deficient mice. Article Snippet: .. The following antibodies were used: microtubule-associated proteins 1 A/1B light chain 3B (LC3; #3868), total STAT3 (#30835S), phosphorylated STAT3 (p-STAT3, Tyr705; #4113S), Kelch-like ECH-associated protein 1 (Keap1; #8047), nuclear factor erythroid 2–related factor 2 (NRF2; #12721), and xanthine dehydrogenase/oxidase (XOR; #53370) from Cell Signaling Technology, MA, USA; Article Title: The effects of the “living high–training low” model on key proteins involved in cellular aerobic and anaerobic metabolism in male C 57 BL /6 J mice Article Snippet: After this, membranes were blocked with 1% milk‐PBS (Nonfat dry milk, cat# 9999, Cell Signaling Technology®) for 1 h at room temperature. .. Regarding primary antibodies, the membranes were incubated with 5% milk in PBS‐Tween for 1 h at room temperature (OXR1, cat# 13514‐1‐AP, Proteintech) or overnight at 4°C (HIF‐1α alpha antibody, cat# NB100‐479, Novus Biologicals and Article Title: Peroxisome Proliferator-Activated Receptor α Deficiency Induces Vascular Pathologies through Endothelial Senescence in Diabetic Retinopathy Article Snippet: Chemiluminescent signals were developed by incubation with Pierce ECL Western Blotting Substrate (Thermo Fisher Scientific), quantified by densitometry using ImageJ version 1.54g, and normalized by β-actin levels. .. The primary antibodies included antibodies against PPARα (Thermo Fisher Scientific; catalog number MA1822; 1:1000), translocase of outer mitochondrial membrane 20 (TOMM20; Abcam, Waltham, MA; catalog number ab186735; 1:1000), Phospho-proteomics:Article Title: Strength training attenuates hepatic inflammation and potentiates mitochondrial adaptations in IL-10-deficient mice. Article Snippet: .. The following antibodies were used: microtubule-associated proteins 1 A/1B light chain 3B (LC3; #3868), total STAT3 (#30835S), phosphorylated STAT3 (p-STAT3, Tyr705; #4113S), Kelch-like ECH-associated protein 1 (Keap1; #8047), nuclear factor erythroid 2–related factor 2 (NRF2; #12721), and xanthine dehydrogenase/oxidase (XOR; #53370) from Cell Signaling Technology, MA, USA; Electrophoresis:Article Title: The Effect of Mechanical Loading on Mitophagy in Aged Myoblasts Article Snippet: Total protein extracts were collected from C2C12 myoblasts using RIPA Buffer (Sigma-Aldrich, St.Louis, MO, USA, R0278-50ML) mixed with phosphatase inhibitor (Cell Signaling Technology, Danvers, MA, USA, #5870S) and protease inhibitor (Cell Signaling Technology, #5871S) Cocktail. .. The extracts underwent electrophoresis (SDS-PAGE), were transferred to a polyvinylidene fluoride (PVDF) membrane, and were blotted with primary [AMPK Rabbit Ab (1:100; Cell Signaling Technology, #5831); p-Ulk1 (Ser 555) Rabbit Ab (1:1000; Cell Signaling Technology, #5869), BNIP3L/Nix Rabbit Ab (1:1000; Cell Signaling Technology, #12396), Parkin Mouse Ab (1:1000; Cell Signaling Technology, #4211); Article Title: The Effect of Mechanical Loading on Mitophagy in Aged Myoblasts. Article Snippet: Total protein extracts were collected from C2C12 myoblasts using RIPA Buffer (SigmaAldrich, St.Louis, MO, USA, R0278-50ML) mixed with phosphatase inhibitor (Cell Signaling Technology, Danvers, MA, USA, #5870S) and protease inhibitor (Cell Signaling Technology, #5871S) Cocktail. .. The extracts underwent electrophoresis (SDS-PAGE), were transferred to a polyvinylidene fluoride (PVDF) membrane, and were blotted with primary [AMPK Rabbit Ab (1:100; Cell Signaling Technology, #5831); p-Ulk1 (Ser 555) Rabbit Ab (1:1000; Cell Signaling Technology, #5869), BNIP3L/Nix Rabbit Ab (1:1000; Cell Signaling Technology, #12396), Parkin Mouse Ab (1:1000; Cell Signaling Technology, #4211); Membrane:Article Title: The Effect of Mechanical Loading on Mitophagy in Aged Myoblasts Article Snippet: Total protein extracts were collected from C2C12 myoblasts using RIPA Buffer (Sigma-Aldrich, St.Louis, MO, USA, R0278-50ML) mixed with phosphatase inhibitor (Cell Signaling Technology, Danvers, MA, USA, #5870S) and protease inhibitor (Cell Signaling Technology, #5871S) Cocktail. .. The extracts underwent electrophoresis (SDS-PAGE), were transferred to a polyvinylidene fluoride (PVDF) membrane, and were blotted with primary [AMPK Rabbit Ab (1:100; Cell Signaling Technology, #5831); p-Ulk1 (Ser 555) Rabbit Ab (1:1000; Cell Signaling Technology, #5869), BNIP3L/Nix Rabbit Ab (1:1000; Cell Signaling Technology, #12396), Parkin Mouse Ab (1:1000; Cell Signaling Technology, #4211); Article Title: The Effect of Mechanical Loading on Mitophagy in Aged Myoblasts. Article Snippet: Total protein extracts were collected from C2C12 myoblasts using RIPA Buffer (SigmaAldrich, St.Louis, MO, USA, R0278-50ML) mixed with phosphatase inhibitor (Cell Signaling Technology, Danvers, MA, USA, #5870S) and protease inhibitor (Cell Signaling Technology, #5871S) Cocktail. .. The extracts underwent electrophoresis (SDS-PAGE), were transferred to a polyvinylidene fluoride (PVDF) membrane, and were blotted with primary [AMPK Rabbit Ab (1:100; Cell Signaling Technology, #5831); p-Ulk1 (Ser 555) Rabbit Ab (1:1000; Cell Signaling Technology, #5869), BNIP3L/Nix Rabbit Ab (1:1000; Cell Signaling Technology, #12396), Parkin Mouse Ab (1:1000; Cell Signaling Technology, #4211); Article Title: Peroxisome Proliferator-Activated Receptor α Deficiency Induces Vascular Pathologies through Endothelial Senescence in Diabetic Retinopathy Article Snippet: Chemiluminescent signals were developed by incubation with Pierce ECL Western Blotting Substrate (Thermo Fisher Scientific), quantified by densitometry using ImageJ version 1.54g, and normalized by β-actin levels. .. The primary antibodies included antibodies against PPARα (Thermo Fisher Scientific; catalog number MA1822; 1:1000), translocase of outer mitochondrial membrane 20 (TOMM20; Abcam, Waltham, MA; catalog number ab186735; 1:1000), Immunodetection:Article Title: The Effect of Mechanical Loading on Mitophagy in Aged Myoblasts Article Snippet: Total protein extracts were collected from C2C12 myoblasts using RIPA Buffer (Sigma-Aldrich, St.Louis, MO, USA, R0278-50ML) mixed with phosphatase inhibitor (Cell Signaling Technology, Danvers, MA, USA, #5870S) and protease inhibitor (Cell Signaling Technology, #5871S) Cocktail. .. The extracts underwent electrophoresis (SDS-PAGE), were transferred to a polyvinylidene fluoride (PVDF) membrane, and were blotted with primary [AMPK Rabbit Ab (1:100; Cell Signaling Technology, #5831); p-Ulk1 (Ser 555) Rabbit Ab (1:1000; Cell Signaling Technology, #5869), BNIP3L/Nix Rabbit Ab (1:1000; Cell Signaling Technology, #12396), Parkin Mouse Ab (1:1000; Cell Signaling Technology, #4211); Article Title: The Effect of Mechanical Loading on Mitophagy in Aged Myoblasts. Article Snippet: Total protein extracts were collected from C2C12 myoblasts using RIPA Buffer (SigmaAldrich, St.Louis, MO, USA, R0278-50ML) mixed with phosphatase inhibitor (Cell Signaling Technology, Danvers, MA, USA, #5870S) and protease inhibitor (Cell Signaling Technology, #5871S) Cocktail. .. The extracts underwent electrophoresis (SDS-PAGE), were transferred to a polyvinylidene fluoride (PVDF) membrane, and were blotted with primary [AMPK Rabbit Ab (1:100; Cell Signaling Technology, #5831); p-Ulk1 (Ser 555) Rabbit Ab (1:1000; Cell Signaling Technology, #5869), BNIP3L/Nix Rabbit Ab (1:1000; Cell Signaling Technology, #12396), Parkin Mouse Ab (1:1000; Cell Signaling Technology, #4211); Incubation:Article Title: The effects of the “living high–training low” model on key proteins involved in cellular aerobic and anaerobic metabolism in male C 57 BL /6 J mice Article Snippet: After this, membranes were blocked with 1% milk‐PBS (Nonfat dry milk, cat# 9999, Cell Signaling Technology®) for 1 h at room temperature. .. Regarding primary antibodies, the membranes were incubated with 5% milk in PBS‐Tween for 1 h at room temperature (OXR1, cat# 13514‐1‐AP, Proteintech) or overnight at 4°C (HIF‐1α alpha antibody, cat# NB100‐479, Novus Biologicals and other:Article Title: OGG1 increases exercise endurance via elevated skeletal muscle FGF21. Article Snippet: Known mechanisms underlying elevated FGF21 in Ogg1Tg mice. (A) Markers of ER stress were assessed using quantitative PCR in the skeletal muscle of WT and Ogg1Tg mice. (B) XBP1 splicing, another ER stress marker was assessed in the skeletal muscle of WT and Ogg1Tg mice. (C) Phosphorylation of Akt was measured using PAGE and images were quantified using ImageJ. (n=5-7); data are expressed as mean ± SD; *p<0.05 relative to WT. |
