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pet30a expression vector  (Millipore)


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    Structured Review

    Millipore pet30a expression vector
    Pet30a Expression Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet30a+expression+vector/pet28a/pmc03464273-227-0-3
    Average 90 stars, based on 1 article reviews
    pet30a expression vector - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: CpgD is a phosphoglycerate cytidylyltransferase required for ceramide diphosphoglycerate synthesis.
    Article Snippet: 180 181 Purification of His-tagged CpgD 182 cpgD was amplified (EK1708/1709) and ligated into the NdeI/HindIII site of pET-28a 183 (EMD Millipore) to yield an N-terminal fusion protein.

    Article Title: Serine/threonine/tyrosine kinase 1 drives pancreatic carcinogenesis via GSK3β sequestration-mediated Wnt/β-catenin pathway hyperactivation.
    Article Snippet: The cDNAs encoding β-catenin or GSK3β were cloned into pET-28a (EMD Biosciences, 69864-3).

    Article Title: Metformin Inhibits Cell Motility and Proliferation of Triple-Negative Breast Cancer Cells by Blocking HMGB1/RAGE Signaling.
    Article Snippet: The full-length form of HMGB1 protein cloned in pET28a+ plasmid (pET-28a(+) DNA (Cat# 69864-3, Novagen®, MilliporeSigma, Burlington, MA, USA) was expressed in modified Escherichia coli BL21 Poly Lys S as previously described [32].

    Article Title: CpgD is a phosphoglycerate cytidylyltransferase required for ceramide diphosphoglycerate synthesis
    Article Snippet: cpgD was amplified (EK1708/1709) and ligated into the NdeI/HindIII site of pET-28a (EMD Millipore) to yield an N-terminal fusion protein.

    Clone Assay:

    Article Title: Solid phase transitions as a solution to the genome folding paradox.
    Article Snippet: Ultra-long-range genomic contacts, which are key components of neuronal genome architecture, constitute a biochemical enigma.. This is because regulatory DNA elements make selective and stable contacts with DNA sequences located hundreds of kilobases away, instead of interacting with proximal sequences occupied by the exact same transcription factors.. This is exemplified in olfactory sensory neurons (OSNs), in which only a fraction of LHX2-, EBF1and LDB1-bound sites interact with each other, converging into highly selective multi-chromosomal enhancer hubs.

    Plasmid Preparation:

    Article Title: Solid phase transitions as a solution to the genome folding paradox.
    Article Snippet: Ultra-long-range genomic contacts, which are key components of neuronal genome architecture, constitute a biochemical enigma.. This is because regulatory DNA elements make selective and stable contacts with DNA sequences located hundreds of kilobases away, instead of interacting with proximal sequences occupied by the exact same transcription factors.. This is exemplified in olfactory sensory neurons (OSNs), in which only a fraction of LHX2-, EBF1and LDB1-bound sites interact with each other, converging into highly selective multi-chromosomal enhancer hubs.

    Article Title: HP1 loses its chromatin clustering and phase separation function across evolution.
    Article Snippet: .. Coding sequences were amplified by PCR and inserted into pET28 backbones (EMD Biosciences) for expression in bacteria and into pSBtet-BP backbones69 (Addgene plasmid #60496) for expression of the proteins with an N-terminal GFP-tag in mammalian cells. pET28 versions for the Nature Communications | (2025) 16:6375 8 expression of the proteins with an N-terminal GFP-tag in bacteria were also constructed. ..

    Article Title: In vitro demonstration and in planta characterization of a condensed, reverse TCA (crTCA) cycle
    Article Snippet: Qiagen pQE vector system was utilized for the overexpression of the crTCA cycle enzymes with His-tags for downstream purification. .. Some of the crTCA enzymes did not express well with the pQE vector and were instead expressed using pET21b or pET28a (MilliporeSigma), as indicated in . ..

    Construct:

    Article Title: Solid phase transitions as a solution to the genome folding paradox.
    Article Snippet: Ultra-long-range genomic contacts, which are key components of neuronal genome architecture, constitute a biochemical enigma.. This is because regulatory DNA elements make selective and stable contacts with DNA sequences located hundreds of kilobases away, instead of interacting with proximal sequences occupied by the exact same transcription factors.. This is exemplified in olfactory sensory neurons (OSNs), in which only a fraction of LHX2-, EBF1and LDB1-bound sites interact with each other, converging into highly selective multi-chromosomal enhancer hubs.

    Article Title: HP1 loses its chromatin clustering and phase separation function across evolution.
    Article Snippet: .. Coding sequences were amplified by PCR and inserted into pET28 backbones (EMD Biosciences) for expression in bacteria and into pSBtet-BP backbones69 (Addgene plasmid #60496) for expression of the proteins with an N-terminal GFP-tag in mammalian cells. pET28 versions for the Nature Communications | (2025) 16:6375 8 expression of the proteins with an N-terminal GFP-tag in bacteria were also constructed. ..

    Amplification:

    Article Title: HP1 loses its chromatin clustering and phase separation function across evolution.
    Article Snippet: .. Coding sequences were amplified by PCR and inserted into pET28 backbones (EMD Biosciences) for expression in bacteria and into pSBtet-BP backbones69 (Addgene plasmid #60496) for expression of the proteins with an N-terminal GFP-tag in mammalian cells. pET28 versions for the Nature Communications | (2025) 16:6375 8 expression of the proteins with an N-terminal GFP-tag in bacteria were also constructed. ..

    Polymerase Chain Reaction:

    Article Title: HP1 loses its chromatin clustering and phase separation function across evolution.
    Article Snippet: .. Coding sequences were amplified by PCR and inserted into pET28 backbones (EMD Biosciences) for expression in bacteria and into pSBtet-BP backbones69 (Addgene plasmid #60496) for expression of the proteins with an N-terminal GFP-tag in mammalian cells. pET28 versions for the Nature Communications | (2025) 16:6375 8 expression of the proteins with an N-terminal GFP-tag in bacteria were also constructed. ..

    Expressing:

    Article Title: HP1 loses its chromatin clustering and phase separation function across evolution.
    Article Snippet: .. Coding sequences were amplified by PCR and inserted into pET28 backbones (EMD Biosciences) for expression in bacteria and into pSBtet-BP backbones69 (Addgene plasmid #60496) for expression of the proteins with an N-terminal GFP-tag in mammalian cells. pET28 versions for the Nature Communications | (2025) 16:6375 8 expression of the proteins with an N-terminal GFP-tag in bacteria were also constructed. ..

    Bacteria:

    Article Title: HP1 loses its chromatin clustering and phase separation function across evolution.
    Article Snippet: .. Coding sequences were amplified by PCR and inserted into pET28 backbones (EMD Biosciences) for expression in bacteria and into pSBtet-BP backbones69 (Addgene plasmid #60496) for expression of the proteins with an N-terminal GFP-tag in mammalian cells. pET28 versions for the Nature Communications | (2025) 16:6375 8 expression of the proteins with an N-terminal GFP-tag in bacteria were also constructed. ..



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