expression vectors pet28a cas9 his (Addgene inc)

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Addgene inc expression vectors pet28a cas9 his
Expression Vectors Pet28a Cas9 His, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet28a+expression+vector/pET28a-Cas9-His+(Plasmid+%2398158)/pm41776280-211-0-3
Average 93 stars, based on 16 article reviews

expression vectors pet28a cas9 his - by Bioz Stars, 2026-10

93/100 stars

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Plasmid Preparation:

Article Title: A Stable and Rapid Protoplast-Based CRISPR/Cas9-Ribonucleoprotein Complex Screening System for Octoploid Strawberry ( Fragaria × ananassa ).
Article Snippet: .. The pET28a-Cas9-His plasmid (plasmid #98158; Addgene, Watertown, MA, USA) encoding Cas9 was used to transform Escherichia coli BL21 Star competent cells (C601003; Thermo Fisher Scientific, Waltham, MA, USA). .. Cas9 was subsequently expressed and purified using Ni-NTA agarose resin specific for His-tagged proteins (30210; Qiagen, Hilden, Germany) as previously described [41]. gRNAs were designed using Cas-Designer [42] to target four copies of strawberry phytoene desaturase (FaPDS) at 4A, 4B, 4C, and 4D chromosomes and one copy of polygalacturonase (FaPG1) genes at 6A chromosome [39].

Article Title: Reversible DNA Translocation as a molecular caliper to probe the nanoscale asymmetry of glass nanopores
Article Snippet: .. The 10 kbp linear DNA was prepared from Cas9-pET28a (Addgene) plasmid by growing and amplifying in a 25 μg/ml kanamycin and extracted by Midi Prep kit (Qiagen). .. The linearization of the plasmid was done by digesting with KpnI-HF restriction enzyme in 10x CutSmart buffer (NEB), resulting in 9564 bp linear DNA.

Article Title: A Stable and Rapid Protoplast-Based CRISPR/Cas9-Ribonucleoprotein Complex Screening System for Octoploid Strawberry ( Fragaria × ananassa )
Article Snippet: .. The pET28a-Cas9-His plasmid (plasmid #98158; Addgene, Watertown, MA, USA) encoding Cas9 was used to transform Escherichia coli BL21 Star competent cells (C601003; Thermo Fisher Scientific, Waltham, MA, USA). .. Cas9 was subsequently expressed and purified using Ni-NTA agarose resin specific for His-tagged proteins (30210; Qiagen, Hilden, Germany) as previously described [ ]. gRNAs were designed using Cas-Designer [ ] to target four copies of strawberry phytoene desaturase ( FaPDS ) at 4A, 4B, 4C, and 4D chromosomes and one copy of polygalacturonase ( FaPG1 ) genes at 6A chromosome [ ].

Article Title: HUH-tagged Cas9 as a platform for efficient ssODN-mediated knock-in via embryo and adult injection in insects.
Article Snippet: The BmN cell line originally from the silkworm Bombyx mori ovary was cultured in SF-900 II medium (ThermoFisher, Cat#10902088) containing 10% fetal bovine serum at 27°C. .. Expression vectors pET28a-Cas9-His (Addgene plasmid #98158)61 and pTD68-PCV-Cas9 (Addgene plasmid #123643)36 were used to produce Cas9 and SUMO-PCV-Cas9 proteins, respectively. .. To express SUMO-Cas9 protein, the expression vector pTD68-SUMO-Cas9 was generated by removing the PCV-tag sequence from pTD68-PCV-Cas9 using KOD -plusMutagenesis Kit (TOYOBO, Cat#SMK-101) (For the detailed Cas9 expression and purification protocol, refer to Supplementary Data 1).

Article Title: Focused Acoustic Vortex-Activated Dual-Stimuli Nanoplatform Synergizes with Checkpoint Blockade to Enhance Macrophage Phagocytosis and Antitumor Immunity.
Article Snippet: Macrophage activation in tumor immunotherapy is hindered by the “do not eat me” evasion mechanism, mediated by the CD47-SIRPα axis.. Current therapeutic strategies that solely block antiphagocytic signals show limited efficacy in solid tumors, indicating the urgent need to simultaneously enhance “eat me” signals.. To this end, we developed a focused acoustic vortex (FAV)-triggered dual-stimuli−responsive nanoplatform to enhance macrophage phagocytosis.

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Article Title: Finely tuned ionizable lipid nanoparticles for CRISPR/Cas9 ribonucleoprotein delivery and gene editing
Article Snippet: Cholesterol, 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), 1,2-dimyristoyl-rac-glycero-3-methoxypolyethylene glycol (DMG-PEG, MW 2000), and 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) were obtained from Avanti Polar Lipids.

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