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GenScript corporation pet-32a expression vector
Pet 32a Expression Vector, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet+expression+vector/pet+32a+expression+vector/pmc09689200-40-16-20
Average 90 stars, based on 1 article reviews
pet-32a expression vector - by Bioz Stars, 2026-11
90/100 stars

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Related Articles

Clone Assay:

Article Title: To Explore the Binding Affinity of Human γ-Secretase Activating Protein (GSAP) Isoform 4 with APP-C99 Peptides
Article Snippet: The GSAP_I4 gene construct in pET-32a(+) ( Figure S2 ) was received from GenScript and was analyzed on 0.8% agarose gel.

Article Title: Exploring Chemokine Homodimer Stability: Structural Insights into CXC and CC Interfaces
Article Snippet: The CXC and CC proteins target genes were designed as described [15] and cloned into a pET-32a Xa/LIC vector (GenScript, NJ, USA).

Article Title: Functionalization of Mesoporous Silica with a G-A-Mismatched dsDNA Chain for Efficient Identification and Selective Capturing of the MutY Protein.
Article Snippet: The Muty (UniProt: P17802) gene (NCBI Gene ID: 947447) was subcloned by Genscript Biotech Co., Ltd. by double digestion with BamHI and XhoI into the N-terminal pET-32a (+) expression vector with His6 tag to obtain plasmid pET-32a (+)-Muty, the plasmid was transfected into E. coli BL21(DE3) receptor cells.

Article Title: Recombinant expression, purification, and characterization of full-length human BST-2 from Escherichia coli.
Article Snippet: HIV-1 virus release from infected cells is blocked by human BST-2, but HIV-1 Vpu efficiently antagonises BST-2 due to direct transmembrane domain interactions that occur between each protein.. Targeting the interaction between these two proteins is seen as viable for HIV-1 antiviral intervention.. This study describes the successful over-expression and purification of a recombinant full-length human BST-2 from inclusion bodies using affinity and anion exchange chromatography.

Article Title: Characterization and protective activity of monoclonal antibodies directed against Fe (3+) ABC transporter substrate-binding protein of Glaesserella parasuis
Article Snippet: The recombinant plasmids named pET-28a-GAPDH, pET-32a-porin and pET-28a-Fe(3+) ABC transporter substrate-binding protein were sequenced (Genscript, Nanjing, China).

Article Title: Molecular mechanisms of centipede toxin SsTx-4 inhibition of inwardly rectifying potassium channels
Article Snippet: SsTx-4’s cDNA with an artificially added enterokinase (EK), and TEV protease recognition sequence at its 5′ end was optimized for recombinant expression in E. coli BL21(DE3), synthesized by GenScript (GenScript Corp), and cloned between the KpnI and BamHI sites in pET-32a(+) (pET-32a-EK/TEV-SsTx-4).

Article Title: Development of a Versatile Half-Strip Lateral Flow Assay toward the Detection of Rift Valley Fever Virus Antibodies
Article Snippet: The full-length sequence of the gene encoding the RVFV nucleocapsid (Genbank accession number NC_014395.1) cloned into pET-32a expression vector by GenScript (Piscataway, NJ, USA) was transformed into competent E. cloni ® EXPRESS BL21 Competent Cells (Lucigen, Middleton, WI, USA) for expression of the RVFV recombinant nucleocapsid protein (rNp) by following the manufacturer’s instructions.

Plasmid Preparation:

Article Title: To Explore the Binding Affinity of Human γ-Secretase Activating Protein (GSAP) Isoform 4 with APP-C99 Peptides
Article Snippet: The GSAP_I4 gene construct in pET-32a(+) ( Figure S2 ) was received from GenScript and was analyzed on 0.8% agarose gel.

Article Title: Exploring Chemokine Homodimer Stability: Structural Insights into CXC and CC Interfaces
Article Snippet: The CXC and CC proteins target genes were designed as described [15] and cloned into a pET-32a Xa/LIC vector (GenScript, NJ, USA).

Article Title: Functionalization of Mesoporous Silica with a G-A-Mismatched dsDNA Chain for Efficient Identification and Selective Capturing of the MutY Protein.
Article Snippet: The Muty (UniProt: P17802) gene (NCBI Gene ID: 947447) was subcloned by Genscript Biotech Co., Ltd. by double digestion with BamHI and XhoI into the N-terminal pET-32a (+) expression vector with His6 tag to obtain plasmid pET-32a (+)-Muty, the plasmid was transfected into E. coli BL21(DE3) receptor cells.

Article Title: Recombinant expression, purification, and characterization of full-length human BST-2 from Escherichia coli.
Article Snippet: HIV-1 virus release from infected cells is blocked by human BST-2, but HIV-1 Vpu efficiently antagonises BST-2 due to direct transmembrane domain interactions that occur between each protein.. Targeting the interaction between these two proteins is seen as viable for HIV-1 antiviral intervention.. This study describes the successful over-expression and purification of a recombinant full-length human BST-2 from inclusion bodies using affinity and anion exchange chromatography.

Article Title: Characterization and protective activity of monoclonal antibodies directed against Fe (3+) ABC transporter substrate-binding protein of Glaesserella parasuis
Article Snippet: The recombinant plasmids named pET-28a-GAPDH, pET-32a-porin and pET-28a-Fe(3+) ABC transporter substrate-binding protein were sequenced (Genscript, Nanjing, China).

Article Title: Molecular mechanisms of centipede toxin SsTx-4 inhibition of inwardly rectifying potassium channels
Article Snippet: SsTx-4’s cDNA with an artificially added enterokinase (EK), and TEV protease recognition sequence at its 5′ end was optimized for recombinant expression in E. coli BL21(DE3), synthesized by GenScript (GenScript Corp), and cloned between the KpnI and BamHI sites in pET-32a(+) (pET-32a-EK/TEV-SsTx-4).

Article Title: Development of a Versatile Half-Strip Lateral Flow Assay toward the Detection of Rift Valley Fever Virus Antibodies
Article Snippet: The full-length sequence of the gene encoding the RVFV nucleocapsid (Genbank accession number NC_014395.1) cloned into pET-32a expression vector by GenScript (Piscataway, NJ, USA) was transformed into competent E. cloni ® EXPRESS BL21 Competent Cells (Lucigen, Middleton, WI, USA) for expression of the RVFV recombinant nucleocapsid protein (rNp) by following the manufacturer’s instructions.

Construct:

Article Title: To Explore the Binding Affinity of Human γ-Secretase Activating Protein (GSAP) Isoform 4 with APP-C99 Peptides
Article Snippet: The GSAP_I4 gene construct in pET-32a(+) ( Figure S2 ) was received from GenScript and was analyzed on 0.8% agarose gel.

Article Title: Exploring Chemokine Homodimer Stability: Structural Insights into CXC and CC Interfaces
Article Snippet: The CXC and CC proteins target genes were designed as described [15] and cloned into a pET-32a Xa/LIC vector (GenScript, NJ, USA).

Article Title: Functionalization of Mesoporous Silica with a G-A-Mismatched dsDNA Chain for Efficient Identification and Selective Capturing of the MutY Protein.
Article Snippet: The Muty (UniProt: P17802) gene (NCBI Gene ID: 947447) was subcloned by Genscript Biotech Co., Ltd. by double digestion with BamHI and XhoI into the N-terminal pET-32a (+) expression vector with His6 tag to obtain plasmid pET-32a (+)-Muty, the plasmid was transfected into E. coli BL21(DE3) receptor cells.

Article Title: Recombinant expression, purification, and characterization of full-length human BST-2 from Escherichia coli.
Article Snippet: HIV-1 virus release from infected cells is blocked by human BST-2, but HIV-1 Vpu efficiently antagonises BST-2 due to direct transmembrane domain interactions that occur between each protein.. Targeting the interaction between these two proteins is seen as viable for HIV-1 antiviral intervention.. This study describes the successful over-expression and purification of a recombinant full-length human BST-2 from inclusion bodies using affinity and anion exchange chromatography.

Article Title: Characterization and protective activity of monoclonal antibodies directed against Fe (3+) ABC transporter substrate-binding protein of Glaesserella parasuis
Article Snippet: The recombinant plasmids named pET-28a-GAPDH, pET-32a-porin and pET-28a-Fe(3+) ABC transporter substrate-binding protein were sequenced (Genscript, Nanjing, China).

Article Title: Molecular mechanisms of centipede toxin SsTx-4 inhibition of inwardly rectifying potassium channels
Article Snippet: SsTx-4’s cDNA with an artificially added enterokinase (EK), and TEV protease recognition sequence at its 5′ end was optimized for recombinant expression in E. coli BL21(DE3), synthesized by GenScript (GenScript Corp), and cloned between the KpnI and BamHI sites in pET-32a(+) (pET-32a-EK/TEV-SsTx-4).

Article Title: Development of a Versatile Half-Strip Lateral Flow Assay toward the Detection of Rift Valley Fever Virus Antibodies
Article Snippet: The full-length sequence of the gene encoding the RVFV nucleocapsid (Genbank accession number NC_014395.1) cloned into pET-32a expression vector by GenScript (Piscataway, NJ, USA) was transformed into competent E. cloni ® EXPRESS BL21 Competent Cells (Lucigen, Middleton, WI, USA) for expression of the RVFV recombinant nucleocapsid protein (rNp) by following the manufacturer’s instructions.

Agarose Gel Electrophoresis:

Article Title: To Explore the Binding Affinity of Human γ-Secretase Activating Protein (GSAP) Isoform 4 with APP-C99 Peptides
Article Snippet: The GSAP_I4 gene construct in pET-32a(+) ( Figure S2 ) was received from GenScript and was analyzed on 0.8% agarose gel.

Article Title: Exploring Chemokine Homodimer Stability: Structural Insights into CXC and CC Interfaces
Article Snippet: The CXC and CC proteins target genes were designed as described [15] and cloned into a pET-32a Xa/LIC vector (GenScript, NJ, USA).

Article Title: Functionalization of Mesoporous Silica with a G-A-Mismatched dsDNA Chain for Efficient Identification and Selective Capturing of the MutY Protein.
Article Snippet: The Muty (UniProt: P17802) gene (NCBI Gene ID: 947447) was subcloned by Genscript Biotech Co., Ltd. by double digestion with BamHI and XhoI into the N-terminal pET-32a (+) expression vector with His6 tag to obtain plasmid pET-32a (+)-Muty, the plasmid was transfected into E. coli BL21(DE3) receptor cells.

Article Title: Recombinant expression, purification, and characterization of full-length human BST-2 from Escherichia coli.
Article Snippet: HIV-1 virus release from infected cells is blocked by human BST-2, but HIV-1 Vpu efficiently antagonises BST-2 due to direct transmembrane domain interactions that occur between each protein.. Targeting the interaction between these two proteins is seen as viable for HIV-1 antiviral intervention.. This study describes the successful over-expression and purification of a recombinant full-length human BST-2 from inclusion bodies using affinity and anion exchange chromatography.

Article Title: Characterization and protective activity of monoclonal antibodies directed against Fe (3+) ABC transporter substrate-binding protein of Glaesserella parasuis
Article Snippet: The recombinant plasmids named pET-28a-GAPDH, pET-32a-porin and pET-28a-Fe(3+) ABC transporter substrate-binding protein were sequenced (Genscript, Nanjing, China).

Article Title: Molecular mechanisms of centipede toxin SsTx-4 inhibition of inwardly rectifying potassium channels
Article Snippet: SsTx-4’s cDNA with an artificially added enterokinase (EK), and TEV protease recognition sequence at its 5′ end was optimized for recombinant expression in E. coli BL21(DE3), synthesized by GenScript (GenScript Corp), and cloned between the KpnI and BamHI sites in pET-32a(+) (pET-32a-EK/TEV-SsTx-4).

Article Title: Development of a Versatile Half-Strip Lateral Flow Assay toward the Detection of Rift Valley Fever Virus Antibodies
Article Snippet: The full-length sequence of the gene encoding the RVFV nucleocapsid (Genbank accession number NC_014395.1) cloned into pET-32a expression vector by GenScript (Piscataway, NJ, USA) was transformed into competent E. cloni ® EXPRESS BL21 Competent Cells (Lucigen, Middleton, WI, USA) for expression of the RVFV recombinant nucleocapsid protein (rNp) by following the manufacturer’s instructions.

Expressing:

Article Title: To Explore the Binding Affinity of Human γ-Secretase Activating Protein (GSAP) Isoform 4 with APP-C99 Peptides
Article Snippet: The GSAP_I4 gene construct in pET-32a(+) ( Figure S2 ) was received from GenScript and was analyzed on 0.8% agarose gel.

Article Title: Exploring Chemokine Homodimer Stability: Structural Insights into CXC and CC Interfaces
Article Snippet: The CXC and CC proteins target genes were designed as described [15] and cloned into a pET-32a Xa/LIC vector (GenScript, NJ, USA).

Article Title: Functionalization of Mesoporous Silica with a G-A-Mismatched dsDNA Chain for Efficient Identification and Selective Capturing of the MutY Protein.
Article Snippet: The Muty (UniProt: P17802) gene (NCBI Gene ID: 947447) was subcloned by Genscript Biotech Co., Ltd. by double digestion with BamHI and XhoI into the N-terminal pET-32a (+) expression vector with His6 tag to obtain plasmid pET-32a (+)-Muty, the plasmid was transfected into E. coli BL21(DE3) receptor cells.

Article Title: Recombinant expression, purification, and characterization of full-length human BST-2 from Escherichia coli.
Article Snippet: HIV-1 virus release from infected cells is blocked by human BST-2, but HIV-1 Vpu efficiently antagonises BST-2 due to direct transmembrane domain interactions that occur between each protein.. Targeting the interaction between these two proteins is seen as viable for HIV-1 antiviral intervention.. This study describes the successful over-expression and purification of a recombinant full-length human BST-2 from inclusion bodies using affinity and anion exchange chromatography.

Article Title: Characterization and protective activity of monoclonal antibodies directed against Fe (3+) ABC transporter substrate-binding protein of Glaesserella parasuis
Article Snippet: The recombinant plasmids named pET-28a-GAPDH, pET-32a-porin and pET-28a-Fe(3+) ABC transporter substrate-binding protein were sequenced (Genscript, Nanjing, China).

Article Title: Molecular mechanisms of centipede toxin SsTx-4 inhibition of inwardly rectifying potassium channels
Article Snippet: SsTx-4’s cDNA with an artificially added enterokinase (EK), and TEV protease recognition sequence at its 5′ end was optimized for recombinant expression in E. coli BL21(DE3), synthesized by GenScript (GenScript Corp), and cloned between the KpnI and BamHI sites in pET-32a(+) (pET-32a-EK/TEV-SsTx-4).

Article Title: Development of a Versatile Half-Strip Lateral Flow Assay toward the Detection of Rift Valley Fever Virus Antibodies
Article Snippet: The full-length sequence of the gene encoding the RVFV nucleocapsid (Genbank accession number NC_014395.1) cloned into pET-32a expression vector by GenScript (Piscataway, NJ, USA) was transformed into competent E. cloni ® EXPRESS BL21 Competent Cells (Lucigen, Middleton, WI, USA) for expression of the RVFV recombinant nucleocapsid protein (rNp) by following the manufacturer’s instructions.

Transfection:

Article Title: To Explore the Binding Affinity of Human γ-Secretase Activating Protein (GSAP) Isoform 4 with APP-C99 Peptides
Article Snippet: The GSAP_I4 gene construct in pET-32a(+) ( Figure S2 ) was received from GenScript and was analyzed on 0.8% agarose gel.

Article Title: Exploring Chemokine Homodimer Stability: Structural Insights into CXC and CC Interfaces
Article Snippet: The CXC and CC proteins target genes were designed as described [15] and cloned into a pET-32a Xa/LIC vector (GenScript, NJ, USA).

Article Title: Functionalization of Mesoporous Silica with a G-A-Mismatched dsDNA Chain for Efficient Identification and Selective Capturing of the MutY Protein.
Article Snippet: The Muty (UniProt: P17802) gene (NCBI Gene ID: 947447) was subcloned by Genscript Biotech Co., Ltd. by double digestion with BamHI and XhoI into the N-terminal pET-32a (+) expression vector with His6 tag to obtain plasmid pET-32a (+)-Muty, the plasmid was transfected into E. coli BL21(DE3) receptor cells.

Article Title: Recombinant expression, purification, and characterization of full-length human BST-2 from Escherichia coli.
Article Snippet: HIV-1 virus release from infected cells is blocked by human BST-2, but HIV-1 Vpu efficiently antagonises BST-2 due to direct transmembrane domain interactions that occur between each protein.. Targeting the interaction between these two proteins is seen as viable for HIV-1 antiviral intervention.. This study describes the successful over-expression and purification of a recombinant full-length human BST-2 from inclusion bodies using affinity and anion exchange chromatography.

Article Title: Characterization and protective activity of monoclonal antibodies directed against Fe (3+) ABC transporter substrate-binding protein of Glaesserella parasuis
Article Snippet: The recombinant plasmids named pET-28a-GAPDH, pET-32a-porin and pET-28a-Fe(3+) ABC transporter substrate-binding protein were sequenced (Genscript, Nanjing, China).

Article Title: Molecular mechanisms of centipede toxin SsTx-4 inhibition of inwardly rectifying potassium channels
Article Snippet: SsTx-4’s cDNA with an artificially added enterokinase (EK), and TEV protease recognition sequence at its 5′ end was optimized for recombinant expression in E. coli BL21(DE3), synthesized by GenScript (GenScript Corp), and cloned between the KpnI and BamHI sites in pET-32a(+) (pET-32a-EK/TEV-SsTx-4).

Article Title: Development of a Versatile Half-Strip Lateral Flow Assay toward the Detection of Rift Valley Fever Virus Antibodies
Article Snippet: The full-length sequence of the gene encoding the RVFV nucleocapsid (Genbank accession number NC_014395.1) cloned into pET-32a expression vector by GenScript (Piscataway, NJ, USA) was transformed into competent E. cloni ® EXPRESS BL21 Competent Cells (Lucigen, Middleton, WI, USA) for expression of the RVFV recombinant nucleocapsid protein (rNp) by following the manufacturer’s instructions.

Sequencing:

Article Title: To Explore the Binding Affinity of Human γ-Secretase Activating Protein (GSAP) Isoform 4 with APP-C99 Peptides
Article Snippet: The GSAP_I4 gene construct in pET-32a(+) ( Figure S2 ) was received from GenScript and was analyzed on 0.8% agarose gel.

Article Title: Exploring Chemokine Homodimer Stability: Structural Insights into CXC and CC Interfaces
Article Snippet: The CXC and CC proteins target genes were designed as described [15] and cloned into a pET-32a Xa/LIC vector (GenScript, NJ, USA).

Article Title: Functionalization of Mesoporous Silica with a G-A-Mismatched dsDNA Chain for Efficient Identification and Selective Capturing of the MutY Protein.
Article Snippet: The Muty (UniProt: P17802) gene (NCBI Gene ID: 947447) was subcloned by Genscript Biotech Co., Ltd. by double digestion with BamHI and XhoI into the N-terminal pET-32a (+) expression vector with His6 tag to obtain plasmid pET-32a (+)-Muty, the plasmid was transfected into E. coli BL21(DE3) receptor cells.

Article Title: Recombinant expression, purification, and characterization of full-length human BST-2 from Escherichia coli.
Article Snippet: HIV-1 virus release from infected cells is blocked by human BST-2, but HIV-1 Vpu efficiently antagonises BST-2 due to direct transmembrane domain interactions that occur between each protein.. Targeting the interaction between these two proteins is seen as viable for HIV-1 antiviral intervention.. This study describes the successful over-expression and purification of a recombinant full-length human BST-2 from inclusion bodies using affinity and anion exchange chromatography.

Article Title: Characterization and protective activity of monoclonal antibodies directed against Fe (3+) ABC transporter substrate-binding protein of Glaesserella parasuis
Article Snippet: The recombinant plasmids named pET-28a-GAPDH, pET-32a-porin and pET-28a-Fe(3+) ABC transporter substrate-binding protein were sequenced (Genscript, Nanjing, China).

Article Title: Molecular mechanisms of centipede toxin SsTx-4 inhibition of inwardly rectifying potassium channels
Article Snippet: SsTx-4’s cDNA with an artificially added enterokinase (EK), and TEV protease recognition sequence at its 5′ end was optimized for recombinant expression in E. coli BL21(DE3), synthesized by GenScript (GenScript Corp), and cloned between the KpnI and BamHI sites in pET-32a(+) (pET-32a-EK/TEV-SsTx-4).

Article Title: Development of a Versatile Half-Strip Lateral Flow Assay toward the Detection of Rift Valley Fever Virus Antibodies
Article Snippet: The full-length sequence of the gene encoding the RVFV nucleocapsid (Genbank accession number NC_014395.1) cloned into pET-32a expression vector by GenScript (Piscataway, NJ, USA) was transformed into competent E. cloni ® EXPRESS BL21 Competent Cells (Lucigen, Middleton, WI, USA) for expression of the RVFV recombinant nucleocapsid protein (rNp) by following the manufacturer’s instructions.

Transformation Assay:

Article Title: To Explore the Binding Affinity of Human γ-Secretase Activating Protein (GSAP) Isoform 4 with APP-C99 Peptides
Article Snippet: The GSAP_I4 gene construct in pET-32a(+) ( Figure S2 ) was received from GenScript and was analyzed on 0.8% agarose gel.

Article Title: Exploring Chemokine Homodimer Stability: Structural Insights into CXC and CC Interfaces
Article Snippet: The CXC and CC proteins target genes were designed as described [15] and cloned into a pET-32a Xa/LIC vector (GenScript, NJ, USA).

Article Title: Functionalization of Mesoporous Silica with a G-A-Mismatched dsDNA Chain for Efficient Identification and Selective Capturing of the MutY Protein.
Article Snippet: The Muty (UniProt: P17802) gene (NCBI Gene ID: 947447) was subcloned by Genscript Biotech Co., Ltd. by double digestion with BamHI and XhoI into the N-terminal pET-32a (+) expression vector with His6 tag to obtain plasmid pET-32a (+)-Muty, the plasmid was transfected into E. coli BL21(DE3) receptor cells.

Article Title: Recombinant expression, purification, and characterization of full-length human BST-2 from Escherichia coli.
Article Snippet: HIV-1 virus release from infected cells is blocked by human BST-2, but HIV-1 Vpu efficiently antagonises BST-2 due to direct transmembrane domain interactions that occur between each protein.. Targeting the interaction between these two proteins is seen as viable for HIV-1 antiviral intervention.. This study describes the successful over-expression and purification of a recombinant full-length human BST-2 from inclusion bodies using affinity and anion exchange chromatography.

Article Title: Characterization and protective activity of monoclonal antibodies directed against Fe (3+) ABC transporter substrate-binding protein of Glaesserella parasuis
Article Snippet: The recombinant plasmids named pET-28a-GAPDH, pET-32a-porin and pET-28a-Fe(3+) ABC transporter substrate-binding protein were sequenced (Genscript, Nanjing, China).

Article Title: Molecular mechanisms of centipede toxin SsTx-4 inhibition of inwardly rectifying potassium channels
Article Snippet: SsTx-4’s cDNA with an artificially added enterokinase (EK), and TEV protease recognition sequence at its 5′ end was optimized for recombinant expression in E. coli BL21(DE3), synthesized by GenScript (GenScript Corp), and cloned between the KpnI and BamHI sites in pET-32a(+) (pET-32a-EK/TEV-SsTx-4).

Article Title: Development of a Versatile Half-Strip Lateral Flow Assay toward the Detection of Rift Valley Fever Virus Antibodies
Article Snippet: The full-length sequence of the gene encoding the RVFV nucleocapsid (Genbank accession number NC_014395.1) cloned into pET-32a expression vector by GenScript (Piscataway, NJ, USA) was transformed into competent E. cloni ® EXPRESS BL21 Competent Cells (Lucigen, Middleton, WI, USA) for expression of the RVFV recombinant nucleocapsid protein (rNp) by following the manufacturer’s instructions.

Recombinant:

Article Title: To Explore the Binding Affinity of Human γ-Secretase Activating Protein (GSAP) Isoform 4 with APP-C99 Peptides
Article Snippet: The GSAP_I4 gene construct in pET-32a(+) ( Figure S2 ) was received from GenScript and was analyzed on 0.8% agarose gel.

Article Title: Exploring Chemokine Homodimer Stability: Structural Insights into CXC and CC Interfaces
Article Snippet: The CXC and CC proteins target genes were designed as described [15] and cloned into a pET-32a Xa/LIC vector (GenScript, NJ, USA).

Article Title: Functionalization of Mesoporous Silica with a G-A-Mismatched dsDNA Chain for Efficient Identification and Selective Capturing of the MutY Protein.
Article Snippet: The Muty (UniProt: P17802) gene (NCBI Gene ID: 947447) was subcloned by Genscript Biotech Co., Ltd. by double digestion with BamHI and XhoI into the N-terminal pET-32a (+) expression vector with His6 tag to obtain plasmid pET-32a (+)-Muty, the plasmid was transfected into E. coli BL21(DE3) receptor cells.

Article Title: Recombinant expression, purification, and characterization of full-length human BST-2 from Escherichia coli.
Article Snippet: HIV-1 virus release from infected cells is blocked by human BST-2, but HIV-1 Vpu efficiently antagonises BST-2 due to direct transmembrane domain interactions that occur between each protein.. Targeting the interaction between these two proteins is seen as viable for HIV-1 antiviral intervention.. This study describes the successful over-expression and purification of a recombinant full-length human BST-2 from inclusion bodies using affinity and anion exchange chromatography.

Article Title: Characterization and protective activity of monoclonal antibodies directed against Fe (3+) ABC transporter substrate-binding protein of Glaesserella parasuis
Article Snippet: The recombinant plasmids named pET-28a-GAPDH, pET-32a-porin and pET-28a-Fe(3+) ABC transporter substrate-binding protein were sequenced (Genscript, Nanjing, China).

Article Title: Molecular mechanisms of centipede toxin SsTx-4 inhibition of inwardly rectifying potassium channels
Article Snippet: SsTx-4’s cDNA with an artificially added enterokinase (EK), and TEV protease recognition sequence at its 5′ end was optimized for recombinant expression in E. coli BL21(DE3), synthesized by GenScript (GenScript Corp), and cloned between the KpnI and BamHI sites in pET-32a(+) (pET-32a-EK/TEV-SsTx-4).

Article Title: Development of a Versatile Half-Strip Lateral Flow Assay toward the Detection of Rift Valley Fever Virus Antibodies
Article Snippet: The full-length sequence of the gene encoding the RVFV nucleocapsid (Genbank accession number NC_014395.1) cloned into pET-32a expression vector by GenScript (Piscataway, NJ, USA) was transformed into competent E. cloni ® EXPRESS BL21 Competent Cells (Lucigen, Middleton, WI, USA) for expression of the RVFV recombinant nucleocapsid protein (rNp) by following the manufacturer’s instructions.

Synthesized:

Article Title: To Explore the Binding Affinity of Human γ-Secretase Activating Protein (GSAP) Isoform 4 with APP-C99 Peptides
Article Snippet: The GSAP_I4 gene construct in pET-32a(+) ( Figure S2 ) was received from GenScript and was analyzed on 0.8% agarose gel.

Article Title: Exploring Chemokine Homodimer Stability: Structural Insights into CXC and CC Interfaces
Article Snippet: The CXC and CC proteins target genes were designed as described [15] and cloned into a pET-32a Xa/LIC vector (GenScript, NJ, USA).

Article Title: Functionalization of Mesoporous Silica with a G-A-Mismatched dsDNA Chain for Efficient Identification and Selective Capturing of the MutY Protein.
Article Snippet: The Muty (UniProt: P17802) gene (NCBI Gene ID: 947447) was subcloned by Genscript Biotech Co., Ltd. by double digestion with BamHI and XhoI into the N-terminal pET-32a (+) expression vector with His6 tag to obtain plasmid pET-32a (+)-Muty, the plasmid was transfected into E. coli BL21(DE3) receptor cells.

Article Title: Recombinant expression, purification, and characterization of full-length human BST-2 from Escherichia coli.
Article Snippet: HIV-1 virus release from infected cells is blocked by human BST-2, but HIV-1 Vpu efficiently antagonises BST-2 due to direct transmembrane domain interactions that occur between each protein.. Targeting the interaction between these two proteins is seen as viable for HIV-1 antiviral intervention.. This study describes the successful over-expression and purification of a recombinant full-length human BST-2 from inclusion bodies using affinity and anion exchange chromatography.

Article Title: Characterization and protective activity of monoclonal antibodies directed against Fe (3+) ABC transporter substrate-binding protein of Glaesserella parasuis
Article Snippet: The recombinant plasmids named pET-28a-GAPDH, pET-32a-porin and pET-28a-Fe(3+) ABC transporter substrate-binding protein were sequenced (Genscript, Nanjing, China).

Article Title: Molecular mechanisms of centipede toxin SsTx-4 inhibition of inwardly rectifying potassium channels
Article Snippet: SsTx-4’s cDNA with an artificially added enterokinase (EK), and TEV protease recognition sequence at its 5′ end was optimized for recombinant expression in E. coli BL21(DE3), synthesized by GenScript (GenScript Corp), and cloned between the KpnI and BamHI sites in pET-32a(+) (pET-32a-EK/TEV-SsTx-4).

Article Title: Development of a Versatile Half-Strip Lateral Flow Assay toward the Detection of Rift Valley Fever Virus Antibodies
Article Snippet: The full-length sequence of the gene encoding the RVFV nucleocapsid (Genbank accession number NC_014395.1) cloned into pET-32a expression vector by GenScript (Piscataway, NJ, USA) was transformed into competent E. cloni ® EXPRESS BL21 Competent Cells (Lucigen, Middleton, WI, USA) for expression of the RVFV recombinant nucleocapsid protein (rNp) by following the manufacturer’s instructions.



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Hypothesis and experiment system (A) We hypothesize that vertical and horizontal gene transfer (VGT and HGT) are influenced by the characteristics of the potential recipient cell types and determine the proliferation and diversity of transconjugant cells. Because the potential recipient community comprises multiple cell types with varying growth traits and conjugation probabilities, we expect the resulting composition of transconjugant cells to be shaped by these cell type-specific traits. (B) Our experimental system consists of E . coli MG1655 lacI q <t>-pLpp-mCherry</t> as the plasmid donor strain and pB10 as the focal plasmid. pB10 donor cells express RFP from the chromosome and transconjugants express GFP from pB10.
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Hypothesis and experiment system (A) We hypothesize that vertical and horizontal gene transfer (VGT and HGT) are influenced by the characteristics of the potential recipient cell types and determine the proliferation and diversity of transconjugant cells. Because the potential recipient community comprises multiple cell types with varying growth traits and conjugation probabilities, we expect the resulting composition of transconjugant cells to be shaped by these cell type-specific traits. (B) Our experimental system consists of E . coli MG1655 lacI q <t>-pLpp-mCherry</t> as the plasmid donor strain and pB10 as the focal plasmid. pB10 donor cells express RFP from the chromosome and transconjugants express GFP from pB10.
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Hypothesis and experiment system (A) We hypothesize that vertical and horizontal gene transfer (VGT and HGT) are influenced by the characteristics of the potential recipient cell types and determine the proliferation and diversity of transconjugant cells. Because the potential recipient community comprises multiple cell types with varying growth traits and conjugation probabilities, we expect the resulting composition of transconjugant cells to be shaped by these cell type-specific traits. (B) Our experimental system consists of E . coli MG1655 lacI q <t>-pLpp-mCherry</t> as the plasmid donor strain and pB10 as the focal plasmid. pB10 donor cells express RFP from the chromosome and transconjugants express GFP from pB10.
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Hypothesis and experiment system (A) We hypothesize that vertical and horizontal gene transfer (VGT and HGT) are influenced by the characteristics of the potential recipient cell types and determine the proliferation and diversity of transconjugant cells. Because the potential recipient community comprises multiple cell types with varying growth traits and conjugation probabilities, we expect the resulting composition of transconjugant cells to be shaped by these cell type-specific traits. (B) Our experimental system consists of E . coli MG1655 lacI q <t>-pLpp-mCherry</t> as the plasmid donor strain and pB10 as the focal plasmid. pB10 donor cells express RFP from the chromosome and transconjugants express GFP from pB10.
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Hypothesis and experiment system (A) We hypothesize that vertical and horizontal gene transfer (VGT and HGT) are influenced by the characteristics of the potential recipient cell types and determine the proliferation and diversity of transconjugant cells. Because the potential recipient community comprises multiple cell types with varying growth traits and conjugation probabilities, we expect the resulting composition of transconjugant cells to be shaped by these cell type-specific traits. (B) Our experimental system consists of E . coli MG1655 lacI q <t>-pLpp-mCherry</t> as the plasmid donor strain and pB10 as the focal plasmid. pB10 donor cells express RFP from the chromosome and transconjugants express GFP from pB10.
Pet 21a 3xnls Spcas9 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Hypothesis and experiment system (A) We hypothesize that vertical and horizontal gene transfer (VGT and HGT) are influenced by the characteristics of the potential recipient cell types and determine the proliferation and diversity of transconjugant cells. Because the potential recipient community comprises multiple cell types with varying growth traits and conjugation probabilities, we expect the resulting composition of transconjugant cells to be shaped by these cell type-specific traits. (B) Our experimental system consists of E . coli MG1655 lacI q <t>-pLpp-mCherry</t> as the plasmid donor strain and pB10 as the focal plasmid. pB10 donor cells express RFP from the chromosome and transconjugants express GFP from pB10.
Pet21a 3xnls Spcas9 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Twist Bioscience pet 28a expression vectors
Hypothesis and experiment system (A) We hypothesize that vertical and horizontal gene transfer (VGT and HGT) are influenced by the characteristics of the potential recipient cell types and determine the proliferation and diversity of transconjugant cells. Because the potential recipient community comprises multiple cell types with varying growth traits and conjugation probabilities, we expect the resulting composition of transconjugant cells to be shaped by these cell type-specific traits. (B) Our experimental system consists of E . coli MG1655 lacI q <t>-pLpp-mCherry</t> as the plasmid donor strain and pB10 as the focal plasmid. pB10 donor cells express RFP from the chromosome and transconjugants express GFP from pB10.
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Addgene inc mbp asn 10 tev 1c expression vector
Hypothesis and experiment system (A) We hypothesize that vertical and horizontal gene transfer (VGT and HGT) are influenced by the characteristics of the potential recipient cell types and determine the proliferation and diversity of transconjugant cells. Because the potential recipient community comprises multiple cell types with varying growth traits and conjugation probabilities, we expect the resulting composition of transconjugant cells to be shaped by these cell type-specific traits. (B) Our experimental system consists of E . coli MG1655 lacI q <t>-pLpp-mCherry</t> as the plasmid donor strain and pB10 as the focal plasmid. pB10 donor cells express RFP from the chromosome and transconjugants express GFP from pB10.
Mbp Asn 10 Tev 1c Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Hypothesis and experiment system (A) We hypothesize that vertical and horizontal gene transfer (VGT and HGT) are influenced by the characteristics of the potential recipient cell types and determine the proliferation and diversity of transconjugant cells. Because the potential recipient community comprises multiple cell types with varying growth traits and conjugation probabilities, we expect the resulting composition of transconjugant cells to be shaped by these cell type-specific traits. (B) Our experimental system consists of E . coli MG1655 lacI q -pLpp-mCherry as the plasmid donor strain and pB10 as the focal plasmid. pB10 donor cells express RFP from the chromosome and transconjugants express GFP from pB10.

Journal: iScience

Article Title: Horizontal and vertical gene transfer shape the plasmid host range in surface-associated microbial systems

doi: 10.1016/j.isci.2026.115299

Figure Lengend Snippet: Hypothesis and experiment system (A) We hypothesize that vertical and horizontal gene transfer (VGT and HGT) are influenced by the characteristics of the potential recipient cell types and determine the proliferation and diversity of transconjugant cells. Because the potential recipient community comprises multiple cell types with varying growth traits and conjugation probabilities, we expect the resulting composition of transconjugant cells to be shaped by these cell type-specific traits. (B) Our experimental system consists of E . coli MG1655 lacI q -pLpp-mCherry as the plasmid donor strain and pB10 as the focal plasmid. pB10 donor cells express RFP from the chromosome and transconjugants express GFP from pB10.

Article Snippet: MBP- mCherry expression plasmid (Amp R ) , Addgene , Plasmid# 29747.

Techniques: Conjugation Assay, Plasmid Preparation

Transconjugant proportions and diversities after surface-associated conjugation assays for different environmental conditions (A) Proportion of transconjugant cells relative to total cells after surface-associated conjugation assays using the WWTP community as the potential recipient cell population. We conducted conjugation assays on 1×SWW, 10×SWW, or LB agar plates using E . coli MG1655 lacI q -pLpp-mCherry as the pB10 donor strain. (B) Relative abundances of bacterial class in the total potential recipient cell population (T) and the transconjugant cell population (TC) as identified by 16S rRNA gene sequencing. We separated and identified TC cells using FC-FACS-sorting of GFP-positive cells. (C) Normalized Shannon index of the transconjugant populations after surface-associated conjugation assays on 1×SWW, 10×SWW, or LB agar plates. We normalized the Shannon index of the TC populations to their corresponding T populations. (D) Principal coordinate analysis (PCoA) based on weighted UniFrac distances of T and TC populations after surface-associated conjugation assays on 1×SWW, 10×SWW, or LB agar plates. (E) Phylogenetic tree of transconjugant ASVs detected after surface-associated conjugation assays on 1×SWW, 10×SWW, or LB agar plates. The outer colored box denotes the bacterial phylum of each ASV, corresponding to the phylum-level groupings shown in panel (B). The inner heatmap box aligned with each tip shows the log 10 fold-changes in ASV abundance (TC relative to T) across the three conditions. For (A and C), each point is an independent biological replicate ( n = 3), horizontal bars are the means, error bars are ±1 standard deviation, and asterisks indicate statistically significant differences between the means based on two-way ANOVA with Holm correction (∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, ns = not significant). For (D), each point is an independent biological replicate ( n = 3).

Journal: iScience

Article Title: Horizontal and vertical gene transfer shape the plasmid host range in surface-associated microbial systems

doi: 10.1016/j.isci.2026.115299

Figure Lengend Snippet: Transconjugant proportions and diversities after surface-associated conjugation assays for different environmental conditions (A) Proportion of transconjugant cells relative to total cells after surface-associated conjugation assays using the WWTP community as the potential recipient cell population. We conducted conjugation assays on 1×SWW, 10×SWW, or LB agar plates using E . coli MG1655 lacI q -pLpp-mCherry as the pB10 donor strain. (B) Relative abundances of bacterial class in the total potential recipient cell population (T) and the transconjugant cell population (TC) as identified by 16S rRNA gene sequencing. We separated and identified TC cells using FC-FACS-sorting of GFP-positive cells. (C) Normalized Shannon index of the transconjugant populations after surface-associated conjugation assays on 1×SWW, 10×SWW, or LB agar plates. We normalized the Shannon index of the TC populations to their corresponding T populations. (D) Principal coordinate analysis (PCoA) based on weighted UniFrac distances of T and TC populations after surface-associated conjugation assays on 1×SWW, 10×SWW, or LB agar plates. (E) Phylogenetic tree of transconjugant ASVs detected after surface-associated conjugation assays on 1×SWW, 10×SWW, or LB agar plates. The outer colored box denotes the bacterial phylum of each ASV, corresponding to the phylum-level groupings shown in panel (B). The inner heatmap box aligned with each tip shows the log 10 fold-changes in ASV abundance (TC relative to T) across the three conditions. For (A and C), each point is an independent biological replicate ( n = 3), horizontal bars are the means, error bars are ±1 standard deviation, and asterisks indicate statistically significant differences between the means based on two-way ANOVA with Holm correction (∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, ns = not significant). For (D), each point is an independent biological replicate ( n = 3).

Article Snippet: MBP- mCherry expression plasmid (Amp R ) , Addgene , Plasmid# 29747.

Techniques: Conjugation Assay, Sequencing, Standard Deviation

Transconjugant growth during surface-associated conjugation assays for different environmental conditions (A) Representative fluorescence microscopy images of transconjugant cells during surface-associated conjugation assays on LB agar plates. E . coli MG1655 lacI q -pLpp-mCherry is the pB10 donor strain and show red fluorescence. Transconjugant cells are green. The time indicated in the images refers to the point at which transconjugant cells first became detectable. (B) Normalized microcolony area ( A / a 0 ) plotted as a function of time during the surface-associated conjugation assays on LB agar plates. A is the total microcolony area and a 0 is the initial transconjugant area. Connected data points are for individual colonies ( n = 12). (C) Microcolony area at the endpoint of the mating assay (t = 24 h) for different environmental conditions. The half-violin and scatterplots present the sample distribution and individual microcolony measurements for surface-associated conjugation assays on different medium (n 1xSWW = 880, n 10xSWW = 664, n LB = 1,070, for microcolony number). We performed each experiment at least three independent experiments. Horizontal bars are the mean microcolony areas, error bars are the 99% confidence intervals, and asterisks indicate statistically significant differences between the means based on two-way ANOVA with Holm correction (∗∗ p < 0.01, ∗∗∗∗ p < 0.0001, ns = not significant).

Journal: iScience

Article Title: Horizontal and vertical gene transfer shape the plasmid host range in surface-associated microbial systems

doi: 10.1016/j.isci.2026.115299

Figure Lengend Snippet: Transconjugant growth during surface-associated conjugation assays for different environmental conditions (A) Representative fluorescence microscopy images of transconjugant cells during surface-associated conjugation assays on LB agar plates. E . coli MG1655 lacI q -pLpp-mCherry is the pB10 donor strain and show red fluorescence. Transconjugant cells are green. The time indicated in the images refers to the point at which transconjugant cells first became detectable. (B) Normalized microcolony area ( A / a 0 ) plotted as a function of time during the surface-associated conjugation assays on LB agar plates. A is the total microcolony area and a 0 is the initial transconjugant area. Connected data points are for individual colonies ( n = 12). (C) Microcolony area at the endpoint of the mating assay (t = 24 h) for different environmental conditions. The half-violin and scatterplots present the sample distribution and individual microcolony measurements for surface-associated conjugation assays on different medium (n 1xSWW = 880, n 10xSWW = 664, n LB = 1,070, for microcolony number). We performed each experiment at least three independent experiments. Horizontal bars are the mean microcolony areas, error bars are the 99% confidence intervals, and asterisks indicate statistically significant differences between the means based on two-way ANOVA with Holm correction (∗∗ p < 0.01, ∗∗∗∗ p < 0.0001, ns = not significant).

Article Snippet: MBP- mCherry expression plasmid (Amp R ) , Addgene , Plasmid# 29747.

Techniques: Conjugation Assay, Fluorescence, Microscopy