pet-32a expression vector (GenScript corporation)
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Pet 32a Expression Vector, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet+expression+vector/pet+32a+expression+vector/pmc09689200-40-16-20
Average 90 stars, based on 1 article reviews
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Clone Assay:Article Title: To Explore the Binding Affinity of Human γ-Secretase Activating Protein (GSAP) Isoform 4 with APP-C99 Peptides Article Snippet: The GSAP_I4 gene construct in pET-32a(+) ( Figure S2 ) was received from Article Title: Exploring Chemokine Homodimer Stability: Structural Insights into CXC and CC Interfaces Article Snippet: The Article Title: Functionalization of Mesoporous Silica with a G-A-Mismatched dsDNA Chain for Efficient Identification and Selective Capturing of the MutY Protein. Article Snippet: The Muty (UniProt: P17802) gene (NCBI Gene ID: 947447) was subcloned by Article Title: Recombinant expression, purification, and characterization of full-length human BST-2 from Escherichia coli. Article Snippet: HIV-1 virus release from infected cells is blocked by human BST-2, but HIV-1 Vpu efficiently antagonises BST-2 due to direct transmembrane domain interactions that occur between each protein.. Targeting the interaction between these two proteins is seen as viable for HIV-1 antiviral intervention.. This study describes the successful over-expression and purification of a recombinant full-length human BST-2 from inclusion bodies using affinity and anion exchange chromatography. Article Title: Characterization and protective activity of monoclonal antibodies directed against Fe (3+) ABC transporter substrate-binding protein of Glaesserella parasuis Article Snippet: The recombinant plasmids named pET-28a-GAPDH, pET-32a-porin and pET-28a-Fe( Article Title: Molecular mechanisms of centipede toxin SsTx-4 inhibition of inwardly rectifying potassium channels Article Snippet: SsTx-4’s cDNA with an artificially added enterokinase (EK), and TEV protease recognition sequence at its 5′ end was optimized for recombinant expression in Article Title: Development of a Versatile Half-Strip Lateral Flow Assay toward the Detection of Rift Valley Fever Virus Antibodies Article Snippet: The full-length sequence of the gene encoding the RVFV nucleocapsid (Genbank accession number NC_014395.1) cloned into pET-32a expression vector by GenScript (Piscataway, NJ, USA) was transformed into competent E. cloni ® EXPRESS BL21 Competent Cells (Lucigen, Middleton, WI, USA) for expression of the RVFV recombinant nucleocapsid protein (rNp) by following the manufacturer’s instructions. Plasmid Preparation:Article Title: To Explore the Binding Affinity of Human γ-Secretase Activating Protein (GSAP) Isoform 4 with APP-C99 Peptides Article Snippet: The GSAP_I4 gene construct in pET-32a(+) ( Figure S2 ) was received from Article Title: Exploring Chemokine Homodimer Stability: Structural Insights into CXC and CC Interfaces Article Snippet: The Article Title: Functionalization of Mesoporous Silica with a G-A-Mismatched dsDNA Chain for Efficient Identification and Selective Capturing of the MutY Protein. Article Snippet: The Muty (UniProt: P17802) gene (NCBI Gene ID: 947447) was subcloned by Article Title: Recombinant expression, purification, and characterization of full-length human BST-2 from Escherichia coli. Article Snippet: HIV-1 virus release from infected cells is blocked by human BST-2, but HIV-1 Vpu efficiently antagonises BST-2 due to direct transmembrane domain interactions that occur between each protein.. Targeting the interaction between these two proteins is seen as viable for HIV-1 antiviral intervention.. This study describes the successful over-expression and purification of a recombinant full-length human BST-2 from inclusion bodies using affinity and anion exchange chromatography. Article Title: Characterization and protective activity of monoclonal antibodies directed against Fe (3+) ABC transporter substrate-binding protein of Glaesserella parasuis Article Snippet: The recombinant plasmids named pET-28a-GAPDH, pET-32a-porin and pET-28a-Fe( Article Title: Molecular mechanisms of centipede toxin SsTx-4 inhibition of inwardly rectifying potassium channels Article Snippet: SsTx-4’s cDNA with an artificially added enterokinase (EK), and TEV protease recognition sequence at its 5′ end was optimized for recombinant expression in Article Title: Development of a Versatile Half-Strip Lateral Flow Assay toward the Detection of Rift Valley Fever Virus Antibodies Article Snippet: The full-length sequence of the gene encoding the RVFV nucleocapsid (Genbank accession number NC_014395.1) cloned into pET-32a expression vector by GenScript (Piscataway, NJ, USA) was transformed into competent E. cloni ® EXPRESS BL21 Competent Cells (Lucigen, Middleton, WI, USA) for expression of the RVFV recombinant nucleocapsid protein (rNp) by following the manufacturer’s instructions. Construct:Article Title: To Explore the Binding Affinity of Human γ-Secretase Activating Protein (GSAP) Isoform 4 with APP-C99 Peptides Article Snippet: The GSAP_I4 gene construct in pET-32a(+) ( Figure S2 ) was received from Article Title: Exploring Chemokine Homodimer Stability: Structural Insights into CXC and CC Interfaces Article Snippet: The Article Title: Functionalization of Mesoporous Silica with a G-A-Mismatched dsDNA Chain for Efficient Identification and Selective Capturing of the MutY Protein. Article Snippet: The Muty (UniProt: P17802) gene (NCBI Gene ID: 947447) was subcloned by Article Title: Recombinant expression, purification, and characterization of full-length human BST-2 from Escherichia coli. Article Snippet: HIV-1 virus release from infected cells is blocked by human BST-2, but HIV-1 Vpu efficiently antagonises BST-2 due to direct transmembrane domain interactions that occur between each protein.. Targeting the interaction between these two proteins is seen as viable for HIV-1 antiviral intervention.. This study describes the successful over-expression and purification of a recombinant full-length human BST-2 from inclusion bodies using affinity and anion exchange chromatography. Article Title: Characterization and protective activity of monoclonal antibodies directed against Fe (3+) ABC transporter substrate-binding protein of Glaesserella parasuis Article Snippet: The recombinant plasmids named pET-28a-GAPDH, pET-32a-porin and pET-28a-Fe( Article Title: Molecular mechanisms of centipede toxin SsTx-4 inhibition of inwardly rectifying potassium channels Article Snippet: SsTx-4’s cDNA with an artificially added enterokinase (EK), and TEV protease recognition sequence at its 5′ end was optimized for recombinant expression in Article Title: Development of a Versatile Half-Strip Lateral Flow Assay toward the Detection of Rift Valley Fever Virus Antibodies Article Snippet: The full-length sequence of the gene encoding the RVFV nucleocapsid (Genbank accession number NC_014395.1) cloned into pET-32a expression vector by GenScript (Piscataway, NJ, USA) was transformed into competent E. cloni ® EXPRESS BL21 Competent Cells (Lucigen, Middleton, WI, USA) for expression of the RVFV recombinant nucleocapsid protein (rNp) by following the manufacturer’s instructions. Agarose Gel Electrophoresis:Article Title: To Explore the Binding Affinity of Human γ-Secretase Activating Protein (GSAP) Isoform 4 with APP-C99 Peptides Article Snippet: The GSAP_I4 gene construct in pET-32a(+) ( Figure S2 ) was received from Article Title: Exploring Chemokine Homodimer Stability: Structural Insights into CXC and CC Interfaces Article Snippet: The Article Title: Functionalization of Mesoporous Silica with a G-A-Mismatched dsDNA Chain for Efficient Identification and Selective Capturing of the MutY Protein. Article Snippet: The Muty (UniProt: P17802) gene (NCBI Gene ID: 947447) was subcloned by Article Title: Recombinant expression, purification, and characterization of full-length human BST-2 from Escherichia coli. Article Snippet: HIV-1 virus release from infected cells is blocked by human BST-2, but HIV-1 Vpu efficiently antagonises BST-2 due to direct transmembrane domain interactions that occur between each protein.. Targeting the interaction between these two proteins is seen as viable for HIV-1 antiviral intervention.. This study describes the successful over-expression and purification of a recombinant full-length human BST-2 from inclusion bodies using affinity and anion exchange chromatography. Article Title: Characterization and protective activity of monoclonal antibodies directed against Fe (3+) ABC transporter substrate-binding protein of Glaesserella parasuis Article Snippet: The recombinant plasmids named pET-28a-GAPDH, pET-32a-porin and pET-28a-Fe( Article Title: Molecular mechanisms of centipede toxin SsTx-4 inhibition of inwardly rectifying potassium channels Article Snippet: SsTx-4’s cDNA with an artificially added enterokinase (EK), and TEV protease recognition sequence at its 5′ end was optimized for recombinant expression in Article Title: Development of a Versatile Half-Strip Lateral Flow Assay toward the Detection of Rift Valley Fever Virus Antibodies Article Snippet: The full-length sequence of the gene encoding the RVFV nucleocapsid (Genbank accession number NC_014395.1) cloned into pET-32a expression vector by GenScript (Piscataway, NJ, USA) was transformed into competent E. cloni ® EXPRESS BL21 Competent Cells (Lucigen, Middleton, WI, USA) for expression of the RVFV recombinant nucleocapsid protein (rNp) by following the manufacturer’s instructions. Expressing:Article Title: To Explore the Binding Affinity of Human γ-Secretase Activating Protein (GSAP) Isoform 4 with APP-C99 Peptides Article Snippet: The GSAP_I4 gene construct in pET-32a(+) ( Figure S2 ) was received from Article Title: Exploring Chemokine Homodimer Stability: Structural Insights into CXC and CC Interfaces Article Snippet: The Article Title: Functionalization of Mesoporous Silica with a G-A-Mismatched dsDNA Chain for Efficient Identification and Selective Capturing of the MutY Protein. Article Snippet: The Muty (UniProt: P17802) gene (NCBI Gene ID: 947447) was subcloned by Article Title: Recombinant expression, purification, and characterization of full-length human BST-2 from Escherichia coli. Article Snippet: HIV-1 virus release from infected cells is blocked by human BST-2, but HIV-1 Vpu efficiently antagonises BST-2 due to direct transmembrane domain interactions that occur between each protein.. Targeting the interaction between these two proteins is seen as viable for HIV-1 antiviral intervention.. This study describes the successful over-expression and purification of a recombinant full-length human BST-2 from inclusion bodies using affinity and anion exchange chromatography. Article Title: Characterization and protective activity of monoclonal antibodies directed against Fe (3+) ABC transporter substrate-binding protein of Glaesserella parasuis Article Snippet: The recombinant plasmids named pET-28a-GAPDH, pET-32a-porin and pET-28a-Fe( Article Title: Molecular mechanisms of centipede toxin SsTx-4 inhibition of inwardly rectifying potassium channels Article Snippet: SsTx-4’s cDNA with an artificially added enterokinase (EK), and TEV protease recognition sequence at its 5′ end was optimized for recombinant expression in Article Title: Development of a Versatile Half-Strip Lateral Flow Assay toward the Detection of Rift Valley Fever Virus Antibodies Article Snippet: The full-length sequence of the gene encoding the RVFV nucleocapsid (Genbank accession number NC_014395.1) cloned into pET-32a expression vector by GenScript (Piscataway, NJ, USA) was transformed into competent E. cloni ® EXPRESS BL21 Competent Cells (Lucigen, Middleton, WI, USA) for expression of the RVFV recombinant nucleocapsid protein (rNp) by following the manufacturer’s instructions. Transfection:Article Title: To Explore the Binding Affinity of Human γ-Secretase Activating Protein (GSAP) Isoform 4 with APP-C99 Peptides Article Snippet: The GSAP_I4 gene construct in pET-32a(+) ( Figure S2 ) was received from Article Title: Exploring Chemokine Homodimer Stability: Structural Insights into CXC and CC Interfaces Article Snippet: The Article Title: Functionalization of Mesoporous Silica with a G-A-Mismatched dsDNA Chain for Efficient Identification and Selective Capturing of the MutY Protein. Article Snippet: The Muty (UniProt: P17802) gene (NCBI Gene ID: 947447) was subcloned by Article Title: Recombinant expression, purification, and characterization of full-length human BST-2 from Escherichia coli. Article Snippet: HIV-1 virus release from infected cells is blocked by human BST-2, but HIV-1 Vpu efficiently antagonises BST-2 due to direct transmembrane domain interactions that occur between each protein.. Targeting the interaction between these two proteins is seen as viable for HIV-1 antiviral intervention.. This study describes the successful over-expression and purification of a recombinant full-length human BST-2 from inclusion bodies using affinity and anion exchange chromatography. Article Title: Characterization and protective activity of monoclonal antibodies directed against Fe (3+) ABC transporter substrate-binding protein of Glaesserella parasuis Article Snippet: The recombinant plasmids named pET-28a-GAPDH, pET-32a-porin and pET-28a-Fe( Article Title: Molecular mechanisms of centipede toxin SsTx-4 inhibition of inwardly rectifying potassium channels Article Snippet: SsTx-4’s cDNA with an artificially added enterokinase (EK), and TEV protease recognition sequence at its 5′ end was optimized for recombinant expression in Article Title: Development of a Versatile Half-Strip Lateral Flow Assay toward the Detection of Rift Valley Fever Virus Antibodies Article Snippet: The full-length sequence of the gene encoding the RVFV nucleocapsid (Genbank accession number NC_014395.1) cloned into pET-32a expression vector by GenScript (Piscataway, NJ, USA) was transformed into competent E. cloni ® EXPRESS BL21 Competent Cells (Lucigen, Middleton, WI, USA) for expression of the RVFV recombinant nucleocapsid protein (rNp) by following the manufacturer’s instructions. Sequencing:Article Title: To Explore the Binding Affinity of Human γ-Secretase Activating Protein (GSAP) Isoform 4 with APP-C99 Peptides Article Snippet: The GSAP_I4 gene construct in pET-32a(+) ( Figure S2 ) was received from Article Title: Exploring Chemokine Homodimer Stability: Structural Insights into CXC and CC Interfaces Article Snippet: The Article Title: Functionalization of Mesoporous Silica with a G-A-Mismatched dsDNA Chain for Efficient Identification and Selective Capturing of the MutY Protein. Article Snippet: The Muty (UniProt: P17802) gene (NCBI Gene ID: 947447) was subcloned by Article Title: Recombinant expression, purification, and characterization of full-length human BST-2 from Escherichia coli. Article Snippet: HIV-1 virus release from infected cells is blocked by human BST-2, but HIV-1 Vpu efficiently antagonises BST-2 due to direct transmembrane domain interactions that occur between each protein.. Targeting the interaction between these two proteins is seen as viable for HIV-1 antiviral intervention.. This study describes the successful over-expression and purification of a recombinant full-length human BST-2 from inclusion bodies using affinity and anion exchange chromatography. Article Title: Characterization and protective activity of monoclonal antibodies directed against Fe (3+) ABC transporter substrate-binding protein of Glaesserella parasuis Article Snippet: The recombinant plasmids named pET-28a-GAPDH, pET-32a-porin and pET-28a-Fe( Article Title: Molecular mechanisms of centipede toxin SsTx-4 inhibition of inwardly rectifying potassium channels Article Snippet: SsTx-4’s cDNA with an artificially added enterokinase (EK), and TEV protease recognition sequence at its 5′ end was optimized for recombinant expression in Article Title: Development of a Versatile Half-Strip Lateral Flow Assay toward the Detection of Rift Valley Fever Virus Antibodies Article Snippet: The full-length sequence of the gene encoding the RVFV nucleocapsid (Genbank accession number NC_014395.1) cloned into pET-32a expression vector by GenScript (Piscataway, NJ, USA) was transformed into competent E. cloni ® EXPRESS BL21 Competent Cells (Lucigen, Middleton, WI, USA) for expression of the RVFV recombinant nucleocapsid protein (rNp) by following the manufacturer’s instructions. Transformation Assay:Article Title: To Explore the Binding Affinity of Human γ-Secretase Activating Protein (GSAP) Isoform 4 with APP-C99 Peptides Article Snippet: The GSAP_I4 gene construct in pET-32a(+) ( Figure S2 ) was received from Article Title: Exploring Chemokine Homodimer Stability: Structural Insights into CXC and CC Interfaces Article Snippet: The Article Title: Functionalization of Mesoporous Silica with a G-A-Mismatched dsDNA Chain for Efficient Identification and Selective Capturing of the MutY Protein. Article Snippet: The Muty (UniProt: P17802) gene (NCBI Gene ID: 947447) was subcloned by Article Title: Recombinant expression, purification, and characterization of full-length human BST-2 from Escherichia coli. Article Snippet: HIV-1 virus release from infected cells is blocked by human BST-2, but HIV-1 Vpu efficiently antagonises BST-2 due to direct transmembrane domain interactions that occur between each protein.. Targeting the interaction between these two proteins is seen as viable for HIV-1 antiviral intervention.. This study describes the successful over-expression and purification of a recombinant full-length human BST-2 from inclusion bodies using affinity and anion exchange chromatography. Article Title: Characterization and protective activity of monoclonal antibodies directed against Fe (3+) ABC transporter substrate-binding protein of Glaesserella parasuis Article Snippet: The recombinant plasmids named pET-28a-GAPDH, pET-32a-porin and pET-28a-Fe( Article Title: Molecular mechanisms of centipede toxin SsTx-4 inhibition of inwardly rectifying potassium channels Article Snippet: SsTx-4’s cDNA with an artificially added enterokinase (EK), and TEV protease recognition sequence at its 5′ end was optimized for recombinant expression in Article Title: Development of a Versatile Half-Strip Lateral Flow Assay toward the Detection of Rift Valley Fever Virus Antibodies Article Snippet: The full-length sequence of the gene encoding the RVFV nucleocapsid (Genbank accession number NC_014395.1) cloned into pET-32a expression vector by GenScript (Piscataway, NJ, USA) was transformed into competent E. cloni ® EXPRESS BL21 Competent Cells (Lucigen, Middleton, WI, USA) for expression of the RVFV recombinant nucleocapsid protein (rNp) by following the manufacturer’s instructions. Recombinant:Article Title: To Explore the Binding Affinity of Human γ-Secretase Activating Protein (GSAP) Isoform 4 with APP-C99 Peptides Article Snippet: The GSAP_I4 gene construct in pET-32a(+) ( Figure S2 ) was received from Article Title: Exploring Chemokine Homodimer Stability: Structural Insights into CXC and CC Interfaces Article Snippet: The Article Title: Functionalization of Mesoporous Silica with a G-A-Mismatched dsDNA Chain for Efficient Identification and Selective Capturing of the MutY Protein. Article Snippet: The Muty (UniProt: P17802) gene (NCBI Gene ID: 947447) was subcloned by Article Title: Recombinant expression, purification, and characterization of full-length human BST-2 from Escherichia coli. Article Snippet: HIV-1 virus release from infected cells is blocked by human BST-2, but HIV-1 Vpu efficiently antagonises BST-2 due to direct transmembrane domain interactions that occur between each protein.. Targeting the interaction between these two proteins is seen as viable for HIV-1 antiviral intervention.. This study describes the successful over-expression and purification of a recombinant full-length human BST-2 from inclusion bodies using affinity and anion exchange chromatography. Article Title: Characterization and protective activity of monoclonal antibodies directed against Fe (3+) ABC transporter substrate-binding protein of Glaesserella parasuis Article Snippet: The recombinant plasmids named pET-28a-GAPDH, pET-32a-porin and pET-28a-Fe( Article Title: Molecular mechanisms of centipede toxin SsTx-4 inhibition of inwardly rectifying potassium channels Article Snippet: SsTx-4’s cDNA with an artificially added enterokinase (EK), and TEV protease recognition sequence at its 5′ end was optimized for recombinant expression in Article Title: Development of a Versatile Half-Strip Lateral Flow Assay toward the Detection of Rift Valley Fever Virus Antibodies Article Snippet: The full-length sequence of the gene encoding the RVFV nucleocapsid (Genbank accession number NC_014395.1) cloned into pET-32a expression vector by GenScript (Piscataway, NJ, USA) was transformed into competent E. cloni ® EXPRESS BL21 Competent Cells (Lucigen, Middleton, WI, USA) for expression of the RVFV recombinant nucleocapsid protein (rNp) by following the manufacturer’s instructions. Synthesized:Article Title: To Explore the Binding Affinity of Human γ-Secretase Activating Protein (GSAP) Isoform 4 with APP-C99 Peptides Article Snippet: The GSAP_I4 gene construct in pET-32a(+) ( Figure S2 ) was received from Article Title: Exploring Chemokine Homodimer Stability: Structural Insights into CXC and CC Interfaces Article Snippet: The Article Title: Functionalization of Mesoporous Silica with a G-A-Mismatched dsDNA Chain for Efficient Identification and Selective Capturing of the MutY Protein. Article Snippet: The Muty (UniProt: P17802) gene (NCBI Gene ID: 947447) was subcloned by Article Title: Recombinant expression, purification, and characterization of full-length human BST-2 from Escherichia coli. Article Snippet: HIV-1 virus release from infected cells is blocked by human BST-2, but HIV-1 Vpu efficiently antagonises BST-2 due to direct transmembrane domain interactions that occur between each protein.. Targeting the interaction between these two proteins is seen as viable for HIV-1 antiviral intervention.. This study describes the successful over-expression and purification of a recombinant full-length human BST-2 from inclusion bodies using affinity and anion exchange chromatography. Article Title: Characterization and protective activity of monoclonal antibodies directed against Fe (3+) ABC transporter substrate-binding protein of Glaesserella parasuis Article Snippet: The recombinant plasmids named pET-28a-GAPDH, pET-32a-porin and pET-28a-Fe( Article Title: Molecular mechanisms of centipede toxin SsTx-4 inhibition of inwardly rectifying potassium channels Article Snippet: SsTx-4’s cDNA with an artificially added enterokinase (EK), and TEV protease recognition sequence at its 5′ end was optimized for recombinant expression in Article Title: Development of a Versatile Half-Strip Lateral Flow Assay toward the Detection of Rift Valley Fever Virus Antibodies Article Snippet: The full-length sequence of the gene encoding the RVFV nucleocapsid (Genbank accession number NC_014395.1) cloned into pET-32a expression vector by GenScript (Piscataway, NJ, USA) was transformed into competent E. cloni ® EXPRESS BL21 Competent Cells (Lucigen, Middleton, WI, USA) for expression of the RVFV recombinant nucleocapsid protein (rNp) by following the manufacturer’s instructions. |
