pet his6 mbp asn10 tev 1c expression vector (Addgene inc)
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Pet His6 Mbp Asn10 Tev 1c Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet+expression+vector/pET+His6+MBP+Asn10+TEV+LIC+cloning+vector+(1C)+(Plasmid+%2329654)/pm41266376-199-3-15
Average 93 stars, based on 21 article reviews
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Expressing:Article Title: Discovery of first-in-class inhibitors of the TRF1:TIN2 protein:protein interaction by fragment screening Article Snippet: .. In brief, a pET-His 6 - Article Title: Discovery of first-in-class inhibitors of the TRF1:TIN2 protein:protein interaction by fragment screening. Article Snippet: .. In brief, a Article Title: Discovery of a tRNA-regulatory transcription factor that suppresses breast cancer metastasis Article Snippet: .. E. coli codon-optimized DNA sequences coding ZZEF1 domains (EF hand (111-146 amino acid), DOC (226-405 amino acid), ZZ1 (1778-1833 amino acid), ZZ2 (1827-1882 amino acid), ZZ1/2 (1778-1882 amino acid)) were synthesized and cloned into a Plasmid Preparation:Article Title: Discovery of first-in-class inhibitors of the TRF1:TIN2 protein:protein interaction by fragment screening Article Snippet: .. In brief, a pET-His 6 - Article Title: Structural basis of RECQL5-induced RNA polymerase II transcription braking and subsequent reactivation. Article Snippet: The sequence for human Pol II CTD was amplified from the codon-optimized RPB1 sequence (GenScript). .. Sequences for RECQL5, Pol II CTD, CSB and CUL4A were cloned into the 438C vector (Addgene, 55220) with an N-terminal His6-MBP tag; those for CSA and RBX1 were cloned into the 438A vector with no tag (Addgene, 55218); those for DDB1 and UVSSA were cloned into the 438B vector (Addgene, 55219) with an N-terminal His6 tag, and those for ELOF1 and UbcH5b were cloned into the Article Title: Structural basis for CTCF-mediated chromatin organization Article Snippet: .. CTCF ZnF1-11 (residues Lys263-Asp581) construct was cloned into Article Title: Structural basis of RECQL5-induced RNA polymerase II transcription braking and subsequent reactivation Article Snippet: The sequence for human Pol II CTD was amplified from the codon-optimized RPB1 sequence (GenScript). .. Sequences for RECQL5, Pol II CTD, CSB and CUL4A were cloned into the 438C vector (Addgene, 55220) with an N-terminal His 6 -MBP tag; those for CSA and RBX1 were cloned into the 438A vector with no tag (Addgene, 55218); those for DDB1 and UVSSA were cloned into the 438B vector (Addgene, 55219) with an N-terminal His 6 tag, and those for ELOF1 and UbcH5b were cloned into the Article Title: Structure of a transcribing Pol II-DSIF-SPT6-U1 snRNP complex. Article Snippet: .. Human U1A, U1C, and U1-70KRRM were amplified from human cDNA and cloned into Article Title: Discovery of first-in-class inhibitors of the TRF1:TIN2 protein:protein interaction by fragment screening. Article Snippet: .. In brief, a Clone Assay:Article Title: Structural basis of RECQL5-induced RNA polymerase II transcription braking and subsequent reactivation. Article Snippet: The sequence for human Pol II CTD was amplified from the codon-optimized RPB1 sequence (GenScript). .. Sequences for RECQL5, Pol II CTD, CSB and CUL4A were cloned into the 438C vector (Addgene, 55220) with an N-terminal His6-MBP tag; those for CSA and RBX1 were cloned into the 438A vector with no tag (Addgene, 55218); those for DDB1 and UVSSA were cloned into the 438B vector (Addgene, 55219) with an N-terminal His6 tag, and those for ELOF1 and UbcH5b were cloned into the Article Title: Structural basis for CTCF-mediated chromatin organization Article Snippet: .. CTCF ZnF1-11 (residues Lys263-Asp581) construct was cloned into Article Title: Structural basis of RECQL5-induced RNA polymerase II transcription braking and subsequent reactivation Article Snippet: The sequence for human Pol II CTD was amplified from the codon-optimized RPB1 sequence (GenScript). .. Sequences for RECQL5, Pol II CTD, CSB and CUL4A were cloned into the 438C vector (Addgene, 55220) with an N-terminal His 6 -MBP tag; those for CSA and RBX1 were cloned into the 438A vector with no tag (Addgene, 55218); those for DDB1 and UVSSA were cloned into the 438B vector (Addgene, 55219) with an N-terminal His 6 tag, and those for ELOF1 and UbcH5b were cloned into the Article Title: Structure of a transcribing Pol II-DSIF-SPT6-U1 snRNP complex. Article Snippet: .. Human U1A, U1C, and U1-70KRRM were amplified from human cDNA and cloned into Article Title: Discovery of a tRNA-regulatory transcription factor that suppresses breast cancer metastasis Article Snippet: .. E. coli codon-optimized DNA sequences coding ZZEF1 domains (EF hand (111-146 amino acid), DOC (226-405 amino acid), ZZ1 (1778-1833 amino acid), ZZ2 (1827-1882 amino acid), ZZ1/2 (1778-1882 amino acid)) were synthesized and cloned into a Ligation:Article Title: Structural basis of RECQL5-induced RNA polymerase II transcription braking and subsequent reactivation. Article Snippet: The sequence for human Pol II CTD was amplified from the codon-optimized RPB1 sequence (GenScript). .. Sequences for RECQL5, Pol II CTD, CSB and CUL4A were cloned into the 438C vector (Addgene, 55220) with an N-terminal His6-MBP tag; those for CSA and RBX1 were cloned into the 438A vector with no tag (Addgene, 55218); those for DDB1 and UVSSA were cloned into the 438B vector (Addgene, 55219) with an N-terminal His6 tag, and those for ELOF1 and UbcH5b were cloned into the Article Title: Structural basis of RECQL5-induced RNA polymerase II transcription braking and subsequent reactivation Article Snippet: The sequence for human Pol II CTD was amplified from the codon-optimized RPB1 sequence (GenScript). .. Sequences for RECQL5, Pol II CTD, CSB and CUL4A were cloned into the 438C vector (Addgene, 55220) with an N-terminal His 6 -MBP tag; those for CSA and RBX1 were cloned into the 438A vector with no tag (Addgene, 55218); those for DDB1 and UVSSA were cloned into the 438B vector (Addgene, 55219) with an N-terminal His 6 tag, and those for ELOF1 and UbcH5b were cloned into the Article Title: Structure of a transcribing Pol II-DSIF-SPT6-U1 snRNP complex. Article Snippet: .. Human U1A, U1C, and U1-70KRRM were amplified from human cDNA and cloned into Article Title: Structural basis of TnsC oligomerization and transposase recruitment in type I-B CRISPR-associated transposons Article Snippet: The DNA sequence encoding Peltigera membranacea cyanobiont 210A (Pmc) TnsC was codon optimized for heterologous expression in E. coli , synthesized by GeneArt with flanking overhangs (Thermo Fisher Scientific) and amplified from the template using the primers TnsC_f and TnsC_r ( ). .. Polymerase chain reaction (PCR) products were inserted into the 1S (Addgene #29659) or Construct:Article Title: Structural basis for CTCF-mediated chromatin organization Article Snippet: .. CTCF ZnF1-11 (residues Lys263-Asp581) construct was cloned into Article Title: Structural basis of TnsC oligomerization and transposase recruitment in type I-B CRISPR-associated transposons Article Snippet: The DNA sequence encoding Peltigera membranacea cyanobiont 210A (Pmc) TnsC was codon optimized for heterologous expression in E. coli , synthesized by GeneArt with flanking overhangs (Thermo Fisher Scientific) and amplified from the template using the primers TnsC_f and TnsC_r ( ). .. Polymerase chain reaction (PCR) products were inserted into the 1S (Addgene #29659) or Cloning:Article Title: Structural basis of RECQL5-induced RNA polymerase II transcription braking and subsequent reactivation Article Snippet: The sequence for human Pol II CTD was amplified from the codon-optimized RPB1 sequence (GenScript). .. Sequences for RECQL5, Pol II CTD, CSB and CUL4A were cloned into the 438C vector (Addgene, 55220) with an N-terminal His 6 -MBP tag; those for CSA and RBX1 were cloned into the 438A vector with no tag (Addgene, 55218); those for DDB1 and UVSSA were cloned into the 438B vector (Addgene, 55219) with an N-terminal His 6 tag, and those for ELOF1 and UbcH5b were cloned into the Article Title: Structural basis of TnsC oligomerization and transposase recruitment in type I-B CRISPR-associated transposons Article Snippet: The DNA sequence encoding Peltigera membranacea cyanobiont 210A (Pmc) TnsC was codon optimized for heterologous expression in E. coli , synthesized by GeneArt with flanking overhangs (Thermo Fisher Scientific) and amplified from the template using the primers TnsC_f and TnsC_r ( ). .. Polymerase chain reaction (PCR) products were inserted into the 1S (Addgene #29659) or Amplification:Article Title: Structure of a transcribing Pol II-DSIF-SPT6-U1 snRNP complex. Article Snippet: .. Human U1A, U1C, and U1-70KRRM were amplified from human cDNA and cloned into Polymerase Chain Reaction:Article Title: Structural basis of TnsC oligomerization and transposase recruitment in type I-B CRISPR-associated transposons Article Snippet: The DNA sequence encoding Peltigera membranacea cyanobiont 210A (Pmc) TnsC was codon optimized for heterologous expression in E. coli , synthesized by GeneArt with flanking overhangs (Thermo Fisher Scientific) and amplified from the template using the primers TnsC_f and TnsC_r ( ). .. Polymerase chain reaction (PCR) products were inserted into the 1S (Addgene #29659) or Virus:Article Title: Structural basis of TnsC oligomerization and transposase recruitment in type I-B CRISPR-associated transposons Article Snippet: The DNA sequence encoding Peltigera membranacea cyanobiont 210A (Pmc) TnsC was codon optimized for heterologous expression in E. coli , synthesized by GeneArt with flanking overhangs (Thermo Fisher Scientific) and amplified from the template using the primers TnsC_f and TnsC_r ( ). .. Polymerase chain reaction (PCR) products were inserted into the 1S (Addgene #29659) or Binding Assay:Article Title: Structural basis of TnsC oligomerization and transposase recruitment in type I-B CRISPR-associated transposons Article Snippet: The DNA sequence encoding Peltigera membranacea cyanobiont 210A (Pmc) TnsC was codon optimized for heterologous expression in E. coli , synthesized by GeneArt with flanking overhangs (Thermo Fisher Scientific) and amplified from the template using the primers TnsC_f and TnsC_r ( ). .. Polymerase chain reaction (PCR) products were inserted into the 1S (Addgene #29659) or Synthesized:Article Title: Discovery of a tRNA-regulatory transcription factor that suppresses breast cancer metastasis Article Snippet: .. E. coli codon-optimized DNA sequences coding ZZEF1 domains (EF hand (111-146 amino acid), DOC (226-405 amino acid), ZZ1 (1778-1833 amino acid), ZZ2 (1827-1882 amino acid), ZZ1/2 (1778-1882 amino acid)) were synthesized and cloned into a |
