Review



pet his6 mbp asn10 tev 1c expression vector  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Addgene inc pet his6 mbp asn10 tev 1c expression vector
    Pet His6 Mbp Asn10 Tev 1c Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet+expression+vector/pET+His6+MBP+Asn10+TEV+LIC+cloning+vector+(1C)+(Plasmid+%2329654)/pm41266376-199-3-15
    Average 93 stars, based on 21 article reviews
    pet his6 mbp asn10 tev 1c expression vector - by Bioz Stars, 2026-10
    93/100 stars

    Images

    Related Articles

    Expressing:

    Article Title: Discovery of first-in-class inhibitors of the TRF1:TIN2 protein:protein interaction by fragment screening
    Article Snippet: .. In brief, a pET-His 6 -MBP-Asn 10 -TEV (1C) expression vector (gift from Dr Scott Gradia, UC Berkeley, via Addgene; #29654) with human TRF1 TRFH cDNA (encoding residues 47–268, UniProt identifier P54274-1, codon-optimised for E. coli and synthesised by GenScript) downstream of an N-terminal His 6 -MBP tag was used to transform E. coli BL21-CodonPlus(DE3)-RIL (Stratagene) for protein expression. ..

    Article Title: Discovery of first-in-class inhibitors of the TRF1:TIN2 protein:protein interaction by fragment screening.
    Article Snippet: .. In brief, a pET-His6-MBP-Asn10-TEV (1C) expression vector (gift from Dr Scott Gradia, UC Berkeley, via Addgene; #29654) with human TRF1TRFH cDNA (encoding residues 47–268, UniProt identifier P54274-1, codon-optimised for E. coli and synthesised by GenScript) downstream of an N-terminal His6-MBP tag was used to transform E. coli BL21-CodonPlus(DE3)-RIL (Stratagene) for protein expression. ..

    Article Title: Discovery of a tRNA-regulatory transcription factor that suppresses breast cancer metastasis
    Article Snippet: .. E. coli codon-optimized DNA sequences coding ZZEF1 domains (EF hand (111-146 amino acid), DOC (226-405 amino acid), ZZ1 (1778-1833 amino acid), ZZ2 (1827-1882 amino acid), ZZ1/2 (1778-1882 amino acid)) were synthesized and cloned into a His-MBP fusion expression vector (Addgene #29654) via BamHI site and Gibson assembly. ..

    Plasmid Preparation:

    Article Title: Discovery of first-in-class inhibitors of the TRF1:TIN2 protein:protein interaction by fragment screening
    Article Snippet: .. In brief, a pET-His 6 -MBP-Asn 10 -TEV (1C) expression vector (gift from Dr Scott Gradia, UC Berkeley, via Addgene; #29654) with human TRF1 TRFH cDNA (encoding residues 47–268, UniProt identifier P54274-1, codon-optimised for E. coli and synthesised by GenScript) downstream of an N-terminal His 6 -MBP tag was used to transform E. coli BL21-CodonPlus(DE3)-RIL (Stratagene) for protein expression. ..

    Article Title: Structural basis of RECQL5-induced RNA polymerase II transcription braking and subsequent reactivation.
    Article Snippet: The sequence for human Pol II CTD was amplified from the codon-optimized RPB1 sequence (GenScript). .. Sequences for RECQL5, Pol II CTD, CSB and CUL4A were cloned into the 438C vector (Addgene, 55220) with an N-terminal His6-MBP tag; those for CSA and RBX1 were cloned into the 438A vector with no tag (Addgene, 55218); those for DDB1 and UVSSA were cloned into the 438B vector (Addgene, 55219) with an N-terminal His6 tag, and those for ELOF1 and UbcH5b were cloned into the 1C vector (Addgene, 29654) with an N-terminal His6-MBP tag using ligation-independent cloning60. ..

    Article Title: Structural basis for CTCF-mediated chromatin organization
    Article Snippet: .. CTCF ZnF1-11 (residues Lys263-Asp581) construct was cloned into vector 1-C (Addgene plasmid 29654) and was fused to an N-terminal 6x His tag, MBP tag, TEV and human rhinovirus 3C protease sites. ..

    Article Title: Structural basis of RECQL5-induced RNA polymerase II transcription braking and subsequent reactivation
    Article Snippet: The sequence for human Pol II CTD was amplified from the codon-optimized RPB1 sequence (GenScript). .. Sequences for RECQL5, Pol II CTD, CSB and CUL4A were cloned into the 438C vector (Addgene, 55220) with an N-terminal His 6 -MBP tag; those for CSA and RBX1 were cloned into the 438A vector with no tag (Addgene, 55218); those for DDB1 and UVSSA were cloned into the 438B vector (Addgene, 55219) with an N-terminal His 6 tag, and those for ELOF1 and UbcH5b were cloned into the 1C vector (Addgene, 29654) with an N-terminal His 6 -MBP tag using ligation-independent cloning . ..

    Article Title: Structure of a transcribing Pol II-DSIF-SPT6-U1 snRNP complex.
    Article Snippet: .. Human U1A, U1C, and U1-70KRRM were amplified from human cDNA and cloned into 1C vector (addgene no. 29654)with anN-terminalHis6MBP-TEV tag using ligation independent cloning42. .. All SPT6 truncations were generated using ligation independent cloning, Quikchange43 or NEB HiFi assembly (SPT6NTD+CTR) into 438C vector (addgene no. 55220) with an N-terminal His6-MBP-TEV tag.

    Article Title: Discovery of first-in-class inhibitors of the TRF1:TIN2 protein:protein interaction by fragment screening.
    Article Snippet: .. In brief, a pET-His6-MBP-Asn10-TEV (1C) expression vector (gift from Dr Scott Gradia, UC Berkeley, via Addgene; #29654) with human TRF1TRFH cDNA (encoding residues 47–268, UniProt identifier P54274-1, codon-optimised for E. coli and synthesised by GenScript) downstream of an N-terminal His6-MBP tag was used to transform E. coli BL21-CodonPlus(DE3)-RIL (Stratagene) for protein expression. ..

    Clone Assay:

    Article Title: Structural basis of RECQL5-induced RNA polymerase II transcription braking and subsequent reactivation.
    Article Snippet: The sequence for human Pol II CTD was amplified from the codon-optimized RPB1 sequence (GenScript). .. Sequences for RECQL5, Pol II CTD, CSB and CUL4A were cloned into the 438C vector (Addgene, 55220) with an N-terminal His6-MBP tag; those for CSA and RBX1 were cloned into the 438A vector with no tag (Addgene, 55218); those for DDB1 and UVSSA were cloned into the 438B vector (Addgene, 55219) with an N-terminal His6 tag, and those for ELOF1 and UbcH5b were cloned into the 1C vector (Addgene, 29654) with an N-terminal His6-MBP tag using ligation-independent cloning60. ..

    Article Title: Structural basis for CTCF-mediated chromatin organization
    Article Snippet: .. CTCF ZnF1-11 (residues Lys263-Asp581) construct was cloned into vector 1-C (Addgene plasmid 29654) and was fused to an N-terminal 6x His tag, MBP tag, TEV and human rhinovirus 3C protease sites. ..

    Article Title: Structural basis of RECQL5-induced RNA polymerase II transcription braking and subsequent reactivation
    Article Snippet: The sequence for human Pol II CTD was amplified from the codon-optimized RPB1 sequence (GenScript). .. Sequences for RECQL5, Pol II CTD, CSB and CUL4A were cloned into the 438C vector (Addgene, 55220) with an N-terminal His 6 -MBP tag; those for CSA and RBX1 were cloned into the 438A vector with no tag (Addgene, 55218); those for DDB1 and UVSSA were cloned into the 438B vector (Addgene, 55219) with an N-terminal His 6 tag, and those for ELOF1 and UbcH5b were cloned into the 1C vector (Addgene, 29654) with an N-terminal His 6 -MBP tag using ligation-independent cloning . ..

    Article Title: Structure of a transcribing Pol II-DSIF-SPT6-U1 snRNP complex.
    Article Snippet: .. Human U1A, U1C, and U1-70KRRM were amplified from human cDNA and cloned into 1C vector (addgene no. 29654)with anN-terminalHis6MBP-TEV tag using ligation independent cloning42. .. All SPT6 truncations were generated using ligation independent cloning, Quikchange43 or NEB HiFi assembly (SPT6NTD+CTR) into 438C vector (addgene no. 55220) with an N-terminal His6-MBP-TEV tag.

    Article Title: Discovery of a tRNA-regulatory transcription factor that suppresses breast cancer metastasis
    Article Snippet: .. E. coli codon-optimized DNA sequences coding ZZEF1 domains (EF hand (111-146 amino acid), DOC (226-405 amino acid), ZZ1 (1778-1833 amino acid), ZZ2 (1827-1882 amino acid), ZZ1/2 (1778-1882 amino acid)) were synthesized and cloned into a His-MBP fusion expression vector (Addgene #29654) via BamHI site and Gibson assembly. ..

    Ligation:

    Article Title: Structural basis of RECQL5-induced RNA polymerase II transcription braking and subsequent reactivation.
    Article Snippet: The sequence for human Pol II CTD was amplified from the codon-optimized RPB1 sequence (GenScript). .. Sequences for RECQL5, Pol II CTD, CSB and CUL4A were cloned into the 438C vector (Addgene, 55220) with an N-terminal His6-MBP tag; those for CSA and RBX1 were cloned into the 438A vector with no tag (Addgene, 55218); those for DDB1 and UVSSA were cloned into the 438B vector (Addgene, 55219) with an N-terminal His6 tag, and those for ELOF1 and UbcH5b were cloned into the 1C vector (Addgene, 29654) with an N-terminal His6-MBP tag using ligation-independent cloning60. ..

    Article Title: Structural basis of RECQL5-induced RNA polymerase II transcription braking and subsequent reactivation
    Article Snippet: The sequence for human Pol II CTD was amplified from the codon-optimized RPB1 sequence (GenScript). .. Sequences for RECQL5, Pol II CTD, CSB and CUL4A were cloned into the 438C vector (Addgene, 55220) with an N-terminal His 6 -MBP tag; those for CSA and RBX1 were cloned into the 438A vector with no tag (Addgene, 55218); those for DDB1 and UVSSA were cloned into the 438B vector (Addgene, 55219) with an N-terminal His 6 tag, and those for ELOF1 and UbcH5b were cloned into the 1C vector (Addgene, 29654) with an N-terminal His 6 -MBP tag using ligation-independent cloning . ..

    Article Title: Structure of a transcribing Pol II-DSIF-SPT6-U1 snRNP complex.
    Article Snippet: .. Human U1A, U1C, and U1-70KRRM were amplified from human cDNA and cloned into 1C vector (addgene no. 29654)with anN-terminalHis6MBP-TEV tag using ligation independent cloning42. .. All SPT6 truncations were generated using ligation independent cloning, Quikchange43 or NEB HiFi assembly (SPT6NTD+CTR) into 438C vector (addgene no. 55220) with an N-terminal His6-MBP-TEV tag.

    Article Title: Structural basis of TnsC oligomerization and transposase recruitment in type I-B CRISPR-associated transposons
    Article Snippet: The DNA sequence encoding Peltigera membranacea cyanobiont 210A (Pmc) TnsC was codon optimized for heterologous expression in E. coli , synthesized by GeneArt with flanking overhangs (Thermo Fisher Scientific) and amplified from the template using the primers TnsC_f and TnsC_r ( ). .. Polymerase chain reaction (PCR) products were inserted into the 1S (Addgene #29659) or 1C (Addgene #29654) vectors using ligation-independent cloning (LIC) [ ], resulting in constructs carrying an N-terminal hexahistidine-SUMO tag (His 6 -SUMO) followed by a tobacco etch virus (TEV) cleavage site or an N-terminal hexahistidine-maltose binding protein tag (His 6 -MBP) followed by a TEV cleavage site, respectively. .. The tnsAB gene was amplified by PCR with the primers TnsAB_f and TnsAB_r ( ) using the vector pHelper (PmcCAST)_PmcPSP1 (Addgene #168151) as a template.

    Construct:

    Article Title: Structural basis for CTCF-mediated chromatin organization
    Article Snippet: .. CTCF ZnF1-11 (residues Lys263-Asp581) construct was cloned into vector 1-C (Addgene plasmid 29654) and was fused to an N-terminal 6x His tag, MBP tag, TEV and human rhinovirus 3C protease sites. ..

    Article Title: Structural basis of TnsC oligomerization and transposase recruitment in type I-B CRISPR-associated transposons
    Article Snippet: The DNA sequence encoding Peltigera membranacea cyanobiont 210A (Pmc) TnsC was codon optimized for heterologous expression in E. coli , synthesized by GeneArt with flanking overhangs (Thermo Fisher Scientific) and amplified from the template using the primers TnsC_f and TnsC_r ( ). .. Polymerase chain reaction (PCR) products were inserted into the 1S (Addgene #29659) or 1C (Addgene #29654) vectors using ligation-independent cloning (LIC) [ ], resulting in constructs carrying an N-terminal hexahistidine-SUMO tag (His 6 -SUMO) followed by a tobacco etch virus (TEV) cleavage site or an N-terminal hexahistidine-maltose binding protein tag (His 6 -MBP) followed by a TEV cleavage site, respectively. .. The tnsAB gene was amplified by PCR with the primers TnsAB_f and TnsAB_r ( ) using the vector pHelper (PmcCAST)_PmcPSP1 (Addgene #168151) as a template.

    Cloning:

    Article Title: Structural basis of RECQL5-induced RNA polymerase II transcription braking and subsequent reactivation
    Article Snippet: The sequence for human Pol II CTD was amplified from the codon-optimized RPB1 sequence (GenScript). .. Sequences for RECQL5, Pol II CTD, CSB and CUL4A were cloned into the 438C vector (Addgene, 55220) with an N-terminal His 6 -MBP tag; those for CSA and RBX1 were cloned into the 438A vector with no tag (Addgene, 55218); those for DDB1 and UVSSA were cloned into the 438B vector (Addgene, 55219) with an N-terminal His 6 tag, and those for ELOF1 and UbcH5b were cloned into the 1C vector (Addgene, 29654) with an N-terminal His 6 -MBP tag using ligation-independent cloning . ..

    Article Title: Structural basis of TnsC oligomerization and transposase recruitment in type I-B CRISPR-associated transposons
    Article Snippet: The DNA sequence encoding Peltigera membranacea cyanobiont 210A (Pmc) TnsC was codon optimized for heterologous expression in E. coli , synthesized by GeneArt with flanking overhangs (Thermo Fisher Scientific) and amplified from the template using the primers TnsC_f and TnsC_r ( ). .. Polymerase chain reaction (PCR) products were inserted into the 1S (Addgene #29659) or 1C (Addgene #29654) vectors using ligation-independent cloning (LIC) [ ], resulting in constructs carrying an N-terminal hexahistidine-SUMO tag (His 6 -SUMO) followed by a tobacco etch virus (TEV) cleavage site or an N-terminal hexahistidine-maltose binding protein tag (His 6 -MBP) followed by a TEV cleavage site, respectively. .. The tnsAB gene was amplified by PCR with the primers TnsAB_f and TnsAB_r ( ) using the vector pHelper (PmcCAST)_PmcPSP1 (Addgene #168151) as a template.

    Amplification:

    Article Title: Structure of a transcribing Pol II-DSIF-SPT6-U1 snRNP complex.
    Article Snippet: .. Human U1A, U1C, and U1-70KRRM were amplified from human cDNA and cloned into 1C vector (addgene no. 29654)with anN-terminalHis6MBP-TEV tag using ligation independent cloning42. .. All SPT6 truncations were generated using ligation independent cloning, Quikchange43 or NEB HiFi assembly (SPT6NTD+CTR) into 438C vector (addgene no. 55220) with an N-terminal His6-MBP-TEV tag.

    Polymerase Chain Reaction:

    Article Title: Structural basis of TnsC oligomerization and transposase recruitment in type I-B CRISPR-associated transposons
    Article Snippet: The DNA sequence encoding Peltigera membranacea cyanobiont 210A (Pmc) TnsC was codon optimized for heterologous expression in E. coli , synthesized by GeneArt with flanking overhangs (Thermo Fisher Scientific) and amplified from the template using the primers TnsC_f and TnsC_r ( ). .. Polymerase chain reaction (PCR) products were inserted into the 1S (Addgene #29659) or 1C (Addgene #29654) vectors using ligation-independent cloning (LIC) [ ], resulting in constructs carrying an N-terminal hexahistidine-SUMO tag (His 6 -SUMO) followed by a tobacco etch virus (TEV) cleavage site or an N-terminal hexahistidine-maltose binding protein tag (His 6 -MBP) followed by a TEV cleavage site, respectively. .. The tnsAB gene was amplified by PCR with the primers TnsAB_f and TnsAB_r ( ) using the vector pHelper (PmcCAST)_PmcPSP1 (Addgene #168151) as a template.

    Virus:

    Article Title: Structural basis of TnsC oligomerization and transposase recruitment in type I-B CRISPR-associated transposons
    Article Snippet: The DNA sequence encoding Peltigera membranacea cyanobiont 210A (Pmc) TnsC was codon optimized for heterologous expression in E. coli , synthesized by GeneArt with flanking overhangs (Thermo Fisher Scientific) and amplified from the template using the primers TnsC_f and TnsC_r ( ). .. Polymerase chain reaction (PCR) products were inserted into the 1S (Addgene #29659) or 1C (Addgene #29654) vectors using ligation-independent cloning (LIC) [ ], resulting in constructs carrying an N-terminal hexahistidine-SUMO tag (His 6 -SUMO) followed by a tobacco etch virus (TEV) cleavage site or an N-terminal hexahistidine-maltose binding protein tag (His 6 -MBP) followed by a TEV cleavage site, respectively. .. The tnsAB gene was amplified by PCR with the primers TnsAB_f and TnsAB_r ( ) using the vector pHelper (PmcCAST)_PmcPSP1 (Addgene #168151) as a template.

    Binding Assay:

    Article Title: Structural basis of TnsC oligomerization and transposase recruitment in type I-B CRISPR-associated transposons
    Article Snippet: The DNA sequence encoding Peltigera membranacea cyanobiont 210A (Pmc) TnsC was codon optimized for heterologous expression in E. coli , synthesized by GeneArt with flanking overhangs (Thermo Fisher Scientific) and amplified from the template using the primers TnsC_f and TnsC_r ( ). .. Polymerase chain reaction (PCR) products were inserted into the 1S (Addgene #29659) or 1C (Addgene #29654) vectors using ligation-independent cloning (LIC) [ ], resulting in constructs carrying an N-terminal hexahistidine-SUMO tag (His 6 -SUMO) followed by a tobacco etch virus (TEV) cleavage site or an N-terminal hexahistidine-maltose binding protein tag (His 6 -MBP) followed by a TEV cleavage site, respectively. .. The tnsAB gene was amplified by PCR with the primers TnsAB_f and TnsAB_r ( ) using the vector pHelper (PmcCAST)_PmcPSP1 (Addgene #168151) as a template.

    Synthesized:

    Article Title: Discovery of a tRNA-regulatory transcription factor that suppresses breast cancer metastasis
    Article Snippet: .. E. coli codon-optimized DNA sequences coding ZZEF1 domains (EF hand (111-146 amino acid), DOC (226-405 amino acid), ZZ1 (1778-1833 amino acid), ZZ2 (1827-1882 amino acid), ZZ1/2 (1778-1882 amino acid)) were synthesized and cloned into a His-MBP fusion expression vector (Addgene #29654) via BamHI site and Gibson assembly. ..



    Similar Products

    94
    Addgene inc mbp mcherry expression plasmid
    Hypothesis and experiment system (A) We hypothesize that vertical and horizontal gene transfer (VGT and HGT) are influenced by the characteristics of the potential recipient cell types and determine the proliferation and diversity of transconjugant cells. Because the potential recipient community comprises multiple cell types with varying growth traits and conjugation probabilities, we expect the resulting composition of transconjugant cells to be shaped by these cell type-specific traits. (B) Our experimental system consists of E . coli MG1655 lacI q <t>-pLpp-mCherry</t> as the plasmid donor strain and pB10 as the focal plasmid. pB10 donor cells express RFP from the chromosome and transconjugants express GFP from pB10.
    Mbp Mcherry Expression Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet+expression+vector/pET+MBP+mCherry+LIC+cloning+vector+(MBP-mCherry)+(Plasmid+%2329747)/pmc13020068-45-0-8
    Average 94 stars, based on 1 article reviews
    mbp mcherry expression plasmid - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    99
    Plasmidsaurus pet bacterial expression vectors
    Hypothesis and experiment system (A) We hypothesize that vertical and horizontal gene transfer (VGT and HGT) are influenced by the characteristics of the potential recipient cell types and determine the proliferation and diversity of transconjugant cells. Because the potential recipient community comprises multiple cell types with varying growth traits and conjugation probabilities, we expect the resulting composition of transconjugant cells to be shaped by these cell type-specific traits. (B) Our experimental system consists of E . coli MG1655 lacI q <t>-pLpp-mCherry</t> as the plasmid donor strain and pB10 as the focal plasmid. pB10 donor cells express RFP from the chromosome and transconjugants express GFP from pB10.
    Pet Bacterial Expression Vectors, supplied by Plasmidsaurus, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet+expression+vector/Bacteria/bio_rxiv__64898__2026__03__04__709699-209-9-8
    Average 99 stars, based on 1 article reviews
    pet bacterial expression vectors - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    94
    Addgene inc pet28a tfam expression vector
    Hypothesis and experiment system (A) We hypothesize that vertical and horizontal gene transfer (VGT and HGT) are influenced by the characteristics of the potential recipient cell types and determine the proliferation and diversity of transconjugant cells. Because the potential recipient community comprises multiple cell types with varying growth traits and conjugation probabilities, we expect the resulting composition of transconjugant cells to be shaped by these cell type-specific traits. (B) Our experimental system consists of E . coli MG1655 lacI q <t>-pLpp-mCherry</t> as the plasmid donor strain and pB10 as the focal plasmid. pB10 donor cells express RFP from the chromosome and transconjugants express GFP from pB10.
    Pet28a Tfam Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet+expression+vector/pET-28a(%2B)-FGF2-K128N+(Plasmid+%23120284)/pm41730877-423-5-8
    Average 94 stars, based on 1 article reviews
    pet28a tfam expression vector - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    93
    Addgene inc untagged 14 a vector
    Hypothesis and experiment system (A) We hypothesize that vertical and horizontal gene transfer (VGT and HGT) are influenced by the characteristics of the potential recipient cell types and determine the proliferation and diversity of transconjugant cells. Because the potential recipient community comprises multiple cell types with varying growth traits and conjugation probabilities, we expect the resulting composition of transconjugant cells to be shaped by these cell type-specific traits. (B) Our experimental system consists of E . coli MG1655 lacI q <t>-pLpp-mCherry</t> as the plasmid donor strain and pB10 as the focal plasmid. pB10 donor cells express RFP from the chromosome and transconjugants express GFP from pB10.
    Untagged 14 A Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet+expression+vector/pET+E%2E+coli+expression+vector+with+BioBrick+polypromoter+restriction+sites+(14-A)+(Plasmid+%2348307)/bio_rxiv__64898__2026__02__18__706689-289-7-10
    Average 93 stars, based on 1 article reviews
    untagged 14 a vector - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    Addgene inc vector 14 c
    Hypothesis and experiment system (A) We hypothesize that vertical and horizontal gene transfer (VGT and HGT) are influenced by the characteristics of the potential recipient cell types and determine the proliferation and diversity of transconjugant cells. Because the potential recipient community comprises multiple cell types with varying growth traits and conjugation probabilities, we expect the resulting composition of transconjugant cells to be shaped by these cell type-specific traits. (B) Our experimental system consists of E . coli MG1655 lacI q <t>-pLpp-mCherry</t> as the plasmid donor strain and pB10 as the focal plasmid. pB10 donor cells express RFP from the chromosome and transconjugants express GFP from pB10.
    Vector 14 C, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet+expression+vector/pET+His6+MBP+TEV+expression+vector+with+BioBrick+polypromoer+restriction+sites+(14-C)+(Plasmid+%2348309)/bio_rxiv__64898__2026__02__06__704447-199-14-16
    Average 93 stars, based on 1 article reviews
    vector 14 c - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    94
    Addgene inc pet 21a 3xnls spcas9 vector
    Hypothesis and experiment system (A) We hypothesize that vertical and horizontal gene transfer (VGT and HGT) are influenced by the characteristics of the potential recipient cell types and determine the proliferation and diversity of transconjugant cells. Because the potential recipient community comprises multiple cell types with varying growth traits and conjugation probabilities, we expect the resulting composition of transconjugant cells to be shaped by these cell type-specific traits. (B) Our experimental system consists of E . coli MG1655 lacI q <t>-pLpp-mCherry</t> as the plasmid donor strain and pB10 as the focal plasmid. pB10 donor cells express RFP from the chromosome and transconjugants express GFP from pB10.
    Pet 21a 3xnls Spcas9 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet+expression+vector/pET-21a_3xNLS_SpCas9_protein_expression+(Plasmid+%23114365)/pmc12866144-503-1-7
    Average 94 stars, based on 1 article reviews
    pet 21a 3xnls spcas9 vector - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    94
    Addgene inc pet21a 3xnls spcas9 vector
    Hypothesis and experiment system (A) We hypothesize that vertical and horizontal gene transfer (VGT and HGT) are influenced by the characteristics of the potential recipient cell types and determine the proliferation and diversity of transconjugant cells. Because the potential recipient community comprises multiple cell types with varying growth traits and conjugation probabilities, we expect the resulting composition of transconjugant cells to be shaped by these cell type-specific traits. (B) Our experimental system consists of E . coli MG1655 lacI q <t>-pLpp-mCherry</t> as the plasmid donor strain and pB10 as the focal plasmid. pB10 donor cells express RFP from the chromosome and transconjugants express GFP from pB10.
    Pet21a 3xnls Spcas9 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet+expression+vector/pET-21a_3xNLS_SpCas9_protein_expression+(Plasmid+%23114365)/pm41512877-343-25-31
    Average 94 stars, based on 1 article reviews
    pet21a 3xnls spcas9 vector - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    86
    Twist Bioscience pet 28a expression vectors
    Hypothesis and experiment system (A) We hypothesize that vertical and horizontal gene transfer (VGT and HGT) are influenced by the characteristics of the potential recipient cell types and determine the proliferation and diversity of transconjugant cells. Because the potential recipient community comprises multiple cell types with varying growth traits and conjugation probabilities, we expect the resulting composition of transconjugant cells to be shaped by these cell type-specific traits. (B) Our experimental system consists of E . coli MG1655 lacI q <t>-pLpp-mCherry</t> as the plasmid donor strain and pB10 as the focal plasmid. pB10 donor cells express RFP from the chromosome and transconjugants express GFP from pB10.
    Pet 28a Expression Vectors, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet+expression+vector/pet28a/bio_rxiv__2025__11__27__690864-94-19-23
    Average 86 stars, based on 1 article reviews
    pet 28a expression vectors - by Bioz Stars, 2026-10
    86/100 stars
      Buy from Supplier

    93
    Addgene inc mbp asn 10 tev 1c expression vector
    Hypothesis and experiment system (A) We hypothesize that vertical and horizontal gene transfer (VGT and HGT) are influenced by the characteristics of the potential recipient cell types and determine the proliferation and diversity of transconjugant cells. Because the potential recipient community comprises multiple cell types with varying growth traits and conjugation probabilities, we expect the resulting composition of transconjugant cells to be shaped by these cell type-specific traits. (B) Our experimental system consists of E . coli MG1655 lacI q <t>-pLpp-mCherry</t> as the plasmid donor strain and pB10 as the focal plasmid. pB10 donor cells express RFP from the chromosome and transconjugants express GFP from pB10.
    Mbp Asn 10 Tev 1c Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet+expression+vector/pET+His6+MBP+Asn10+TEV+LIC+cloning+vector+(1C)+(Plasmid+%2329654)/pmc12635197-217-5-19
    Average 93 stars, based on 1 article reviews
    mbp asn 10 tev 1c expression vector - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    Image Search Results


    Hypothesis and experiment system (A) We hypothesize that vertical and horizontal gene transfer (VGT and HGT) are influenced by the characteristics of the potential recipient cell types and determine the proliferation and diversity of transconjugant cells. Because the potential recipient community comprises multiple cell types with varying growth traits and conjugation probabilities, we expect the resulting composition of transconjugant cells to be shaped by these cell type-specific traits. (B) Our experimental system consists of E . coli MG1655 lacI q -pLpp-mCherry as the plasmid donor strain and pB10 as the focal plasmid. pB10 donor cells express RFP from the chromosome and transconjugants express GFP from pB10.

    Journal: iScience

    Article Title: Horizontal and vertical gene transfer shape the plasmid host range in surface-associated microbial systems

    doi: 10.1016/j.isci.2026.115299

    Figure Lengend Snippet: Hypothesis and experiment system (A) We hypothesize that vertical and horizontal gene transfer (VGT and HGT) are influenced by the characteristics of the potential recipient cell types and determine the proliferation and diversity of transconjugant cells. Because the potential recipient community comprises multiple cell types with varying growth traits and conjugation probabilities, we expect the resulting composition of transconjugant cells to be shaped by these cell type-specific traits. (B) Our experimental system consists of E . coli MG1655 lacI q -pLpp-mCherry as the plasmid donor strain and pB10 as the focal plasmid. pB10 donor cells express RFP from the chromosome and transconjugants express GFP from pB10.

    Article Snippet: MBP- mCherry expression plasmid (Amp R ) , Addgene , Plasmid# 29747.

    Techniques: Conjugation Assay, Plasmid Preparation

    Transconjugant proportions and diversities after surface-associated conjugation assays for different environmental conditions (A) Proportion of transconjugant cells relative to total cells after surface-associated conjugation assays using the WWTP community as the potential recipient cell population. We conducted conjugation assays on 1×SWW, 10×SWW, or LB agar plates using E . coli MG1655 lacI q -pLpp-mCherry as the pB10 donor strain. (B) Relative abundances of bacterial class in the total potential recipient cell population (T) and the transconjugant cell population (TC) as identified by 16S rRNA gene sequencing. We separated and identified TC cells using FC-FACS-sorting of GFP-positive cells. (C) Normalized Shannon index of the transconjugant populations after surface-associated conjugation assays on 1×SWW, 10×SWW, or LB agar plates. We normalized the Shannon index of the TC populations to their corresponding T populations. (D) Principal coordinate analysis (PCoA) based on weighted UniFrac distances of T and TC populations after surface-associated conjugation assays on 1×SWW, 10×SWW, or LB agar plates. (E) Phylogenetic tree of transconjugant ASVs detected after surface-associated conjugation assays on 1×SWW, 10×SWW, or LB agar plates. The outer colored box denotes the bacterial phylum of each ASV, corresponding to the phylum-level groupings shown in panel (B). The inner heatmap box aligned with each tip shows the log 10 fold-changes in ASV abundance (TC relative to T) across the three conditions. For (A and C), each point is an independent biological replicate ( n = 3), horizontal bars are the means, error bars are ±1 standard deviation, and asterisks indicate statistically significant differences between the means based on two-way ANOVA with Holm correction (∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, ns = not significant). For (D), each point is an independent biological replicate ( n = 3).

    Journal: iScience

    Article Title: Horizontal and vertical gene transfer shape the plasmid host range in surface-associated microbial systems

    doi: 10.1016/j.isci.2026.115299

    Figure Lengend Snippet: Transconjugant proportions and diversities after surface-associated conjugation assays for different environmental conditions (A) Proportion of transconjugant cells relative to total cells after surface-associated conjugation assays using the WWTP community as the potential recipient cell population. We conducted conjugation assays on 1×SWW, 10×SWW, or LB agar plates using E . coli MG1655 lacI q -pLpp-mCherry as the pB10 donor strain. (B) Relative abundances of bacterial class in the total potential recipient cell population (T) and the transconjugant cell population (TC) as identified by 16S rRNA gene sequencing. We separated and identified TC cells using FC-FACS-sorting of GFP-positive cells. (C) Normalized Shannon index of the transconjugant populations after surface-associated conjugation assays on 1×SWW, 10×SWW, or LB agar plates. We normalized the Shannon index of the TC populations to their corresponding T populations. (D) Principal coordinate analysis (PCoA) based on weighted UniFrac distances of T and TC populations after surface-associated conjugation assays on 1×SWW, 10×SWW, or LB agar plates. (E) Phylogenetic tree of transconjugant ASVs detected after surface-associated conjugation assays on 1×SWW, 10×SWW, or LB agar plates. The outer colored box denotes the bacterial phylum of each ASV, corresponding to the phylum-level groupings shown in panel (B). The inner heatmap box aligned with each tip shows the log 10 fold-changes in ASV abundance (TC relative to T) across the three conditions. For (A and C), each point is an independent biological replicate ( n = 3), horizontal bars are the means, error bars are ±1 standard deviation, and asterisks indicate statistically significant differences between the means based on two-way ANOVA with Holm correction (∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, ns = not significant). For (D), each point is an independent biological replicate ( n = 3).

    Article Snippet: MBP- mCherry expression plasmid (Amp R ) , Addgene , Plasmid# 29747.

    Techniques: Conjugation Assay, Sequencing, Standard Deviation

    Transconjugant growth during surface-associated conjugation assays for different environmental conditions (A) Representative fluorescence microscopy images of transconjugant cells during surface-associated conjugation assays on LB agar plates. E . coli MG1655 lacI q -pLpp-mCherry is the pB10 donor strain and show red fluorescence. Transconjugant cells are green. The time indicated in the images refers to the point at which transconjugant cells first became detectable. (B) Normalized microcolony area ( A / a 0 ) plotted as a function of time during the surface-associated conjugation assays on LB agar plates. A is the total microcolony area and a 0 is the initial transconjugant area. Connected data points are for individual colonies ( n = 12). (C) Microcolony area at the endpoint of the mating assay (t = 24 h) for different environmental conditions. The half-violin and scatterplots present the sample distribution and individual microcolony measurements for surface-associated conjugation assays on different medium (n 1xSWW = 880, n 10xSWW = 664, n LB = 1,070, for microcolony number). We performed each experiment at least three independent experiments. Horizontal bars are the mean microcolony areas, error bars are the 99% confidence intervals, and asterisks indicate statistically significant differences between the means based on two-way ANOVA with Holm correction (∗∗ p < 0.01, ∗∗∗∗ p < 0.0001, ns = not significant).

    Journal: iScience

    Article Title: Horizontal and vertical gene transfer shape the plasmid host range in surface-associated microbial systems

    doi: 10.1016/j.isci.2026.115299

    Figure Lengend Snippet: Transconjugant growth during surface-associated conjugation assays for different environmental conditions (A) Representative fluorescence microscopy images of transconjugant cells during surface-associated conjugation assays on LB agar plates. E . coli MG1655 lacI q -pLpp-mCherry is the pB10 donor strain and show red fluorescence. Transconjugant cells are green. The time indicated in the images refers to the point at which transconjugant cells first became detectable. (B) Normalized microcolony area ( A / a 0 ) plotted as a function of time during the surface-associated conjugation assays on LB agar plates. A is the total microcolony area and a 0 is the initial transconjugant area. Connected data points are for individual colonies ( n = 12). (C) Microcolony area at the endpoint of the mating assay (t = 24 h) for different environmental conditions. The half-violin and scatterplots present the sample distribution and individual microcolony measurements for surface-associated conjugation assays on different medium (n 1xSWW = 880, n 10xSWW = 664, n LB = 1,070, for microcolony number). We performed each experiment at least three independent experiments. Horizontal bars are the mean microcolony areas, error bars are the 99% confidence intervals, and asterisks indicate statistically significant differences between the means based on two-way ANOVA with Holm correction (∗∗ p < 0.01, ∗∗∗∗ p < 0.0001, ns = not significant).

    Article Snippet: MBP- mCherry expression plasmid (Amp R ) , Addgene , Plasmid# 29747.

    Techniques: Conjugation Assay, Fluorescence, Microscopy