peroxidase-based detection method  (SeraCare Life Sciences)

Journal: Cancer Cell International

Article Title: Expression of osteoprotegerin, receptor activator of nuclear factor kappa-B ligand, tumor necrosis factor-related apoptosis-inducing ligand, stromal cell-derived factor-1 and their receptors in epithelial metastatic breast cancer cell lines

doi: 10.1186/1475-2867-12-29

Figure Lengend Snippet: Expression of membrane RANKL on BC cell lines under the two different culture conditions. ( A ) Immunocytochemistry staining for mRANKL in the MDA-MB-231 cell line under both culture conditions (x 400 magnification). ( B ) Immunocytochemistry staining for mRANKL in the MCF-7 cell line under both culture conditions (x 400). Positive controls were performed in both BC cell lines incubated with anti-RANKL (cytoplasmatic RANKL = cRANKL, evaluated previously) and anti-AE1AE3 (a pan-cytokeratin epithelial marker). No staining was observed in both BC cell lines incubated with an irrelevant rabbit Ig Ab as a negative isotype control. Nuclei were counterstained with hematoxylin (purple).

Article Snippet: According to the manufacturer’s recommendations, a peroxidase-based immunocytochemistry staining method (K0690, Dako) was used for primary Ab detection, and a 3,3′-diaminobenzidine tetrahydrochloride (DAB) substrate system (K3468, Dako) was used as the chromogen.

Techniques: Expressing, Immunocytochemistry, Staining, Incubation, Marker

Journal: Cancer Cell International

Article Title: Expression of osteoprotegerin, receptor activator of nuclear factor kappa-B ligand, tumor necrosis factor-related apoptosis-inducing ligand, stromal cell-derived factor-1 and their receptors in epithelial metastatic breast cancer cell lines

doi: 10.1186/1475-2867-12-29

Figure Lengend Snippet: Expression of membrane and cytoplasmic RANK (mRANK/cRANK), SDF-1 and CXCR4 in both BC cell lines. ( A ) Immunocytochemistry staining for RANK, SDF-1 and CXCR4 in MDA-MB-231 and MCF-7 cells grown under arrest and post-stimulation culture conditions (x 400 magnification). ( B ) The MDA-MB-231 and MCF-7 cell lines were incubated with irrelevant IgG1 and mouse Igs Abs as negative isotype controls (x 400 magnification). Nuclei were counterstained with hematoxylin (purple).

Article Snippet: According to the manufacturer’s recommendations, a peroxidase-based immunocytochemistry staining method (K0690, Dako) was used for primary Ab detection, and a 3,3′-diaminobenzidine tetrahydrochloride (DAB) substrate system (K3468, Dako) was used as the chromogen.

Techniques: Expressing, Immunocytochemistry, Staining, Incubation

Journal: Cancer Cell International

Article Title: Expression of osteoprotegerin, receptor activator of nuclear factor kappa-B ligand, tumor necrosis factor-related apoptosis-inducing ligand, stromal cell-derived factor-1 and their receptors in epithelial metastatic breast cancer cell lines

doi: 10.1186/1475-2867-12-29

Figure Lengend Snippet: Expression of TRAIL, TRAIL-R1, TRAIL-R2, TRAIL-R3 and TRAIL-R4 in both BC cell lines. ( A ) Immunocytochemistry staining for TRAIL, TRAIL-R1, TRAIL-R2, TRAIL-R3 and TRAIL-R4 in the MDA-MB-231 and MCF-7 cell lines grown under arrest and post-stimulation culture conditions (x 400 magnification). ( B ) The MDA-MB-231 and MCF-7 cell lines were incubated with anti-AE1AE3 (positive control) and with irrelevant mouse Igs, goat IgG and IgG1 Abs as negative isotype controls (x 400 magnification). Nuclei were counterstained with hematoxylin (purple).

Article Snippet: According to the manufacturer’s recommendations, a peroxidase-based immunocytochemistry staining method (K0690, Dako) was used for primary Ab detection, and a 3,3′-diaminobenzidine tetrahydrochloride (DAB) substrate system (K3468, Dako) was used as the chromogen.

Techniques: Expressing, Immunocytochemistry, Staining, Incubation, Positive Control