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pepperell permeable cell culture inserts 3 0 µm  (CELLTREAT Scientific)


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    Structured Review

    CELLTREAT Scientific pepperell permeable cell culture inserts 3 0 µm
    Pepperell Permeable Cell Culture Inserts 3 0 µm, supplied by CELLTREAT Scientific, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/permeable+inserts/Permeable+Cell+Culture+Inserts/pm36976763-305-4-0
    Average 94 stars, based on 9 article reviews
    pepperell permeable cell culture inserts 3 0 µm - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Suspension:

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries.
    Article Snippet: .. A 3 μL aliquot of this bacterial suspension was carefully pipetted onto a sterile filter membrane of cell culture inserts (0.4 μm pore size, PET membrane, sterile, packed in 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA). ..

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries.
    Article Snippet: .. A 3 μL aliquot of the bacterial suspension was carefully pipetted onto permeable cell culture inserts (0.4-μm pore size, PET membrane, sterile, packed in 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA) positioned on MH agar plates in sterile polystyrene Petri dishes (100 mm× 15mm) with clear lids (FisherbrandTM, Thermo Fisher Scientific, Waltham, MA, USA). ..

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries
    Article Snippet: .. A 3 μL aliquot of the bacterial suspension was carefully pipetted onto permeable cell culture inserts (0.4-μm pore size, PET membrane, sterile, packed in 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA) positioned on MH agar plates in sterile polystyrene Petri dishes (100 mm × 15 mm) with clear lids (FisherbrandTM, Thermo Fisher Scientific, Waltham, MA, USA). ..

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries
    Article Snippet: .. A 3 μL aliquot of this bacterial suspension was carefully pipetted onto a sterile filter membrane of cell culture inserts (0.4 μm pore size, PET membrane, sterile, packed in 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA). ..

    Sterility:

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries.
    Article Snippet: .. A 3 μL aliquot of this bacterial suspension was carefully pipetted onto a sterile filter membrane of cell culture inserts (0.4 μm pore size, PET membrane, sterile, packed in 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA). ..

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries.
    Article Snippet: .. A 3 μL aliquot of the bacterial suspension was carefully pipetted onto permeable cell culture inserts (0.4-μm pore size, PET membrane, sterile, packed in 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA) positioned on MH agar plates in sterile polystyrene Petri dishes (100 mm× 15mm) with clear lids (FisherbrandTM, Thermo Fisher Scientific, Waltham, MA, USA). ..

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries
    Article Snippet: .. A 3 μL aliquot of the bacterial suspension was carefully pipetted onto permeable cell culture inserts (0.4-μm pore size, PET membrane, sterile, packed in 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA) positioned on MH agar plates in sterile polystyrene Petri dishes (100 mm × 15 mm) with clear lids (FisherbrandTM, Thermo Fisher Scientific, Waltham, MA, USA). ..

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries
    Article Snippet: .. A 3 μL aliquot of this bacterial suspension was carefully pipetted onto a sterile filter membrane of cell culture inserts (0.4 μm pore size, PET membrane, sterile, packed in 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA). ..

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries.
    Article Snippet: .. Sterile cell culture inserts (0.4 μm pore size, PET membrane, sterile, packed in 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA) were carefully positioned on the MH agar plates, ensuring that substrate stiffness does not affect the experiment. ..

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries
    Article Snippet: .. Sterile cell culture inserts (0.4 μm pore size, PET membrane, sterile, packed in 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA) were carefully positioned on the MH agar plates, ensuring that substrate stiffness does not affect the experiment. ..

    Membrane:

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries.
    Article Snippet: .. A 3 μL aliquot of this bacterial suspension was carefully pipetted onto a sterile filter membrane of cell culture inserts (0.4 μm pore size, PET membrane, sterile, packed in 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA). ..

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries.
    Article Snippet: .. A 3 μL aliquot of the bacterial suspension was carefully pipetted onto permeable cell culture inserts (0.4-μm pore size, PET membrane, sterile, packed in 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA) positioned on MH agar plates in sterile polystyrene Petri dishes (100 mm× 15mm) with clear lids (FisherbrandTM, Thermo Fisher Scientific, Waltham, MA, USA). ..

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries
    Article Snippet: .. A 3 μL aliquot of the bacterial suspension was carefully pipetted onto permeable cell culture inserts (0.4-μm pore size, PET membrane, sterile, packed in 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA) positioned on MH agar plates in sterile polystyrene Petri dishes (100 mm × 15 mm) with clear lids (FisherbrandTM, Thermo Fisher Scientific, Waltham, MA, USA). ..

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries
    Article Snippet: .. A 3 μL aliquot of this bacterial suspension was carefully pipetted onto a sterile filter membrane of cell culture inserts (0.4 μm pore size, PET membrane, sterile, packed in 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA). ..

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries.
    Article Snippet: .. Sterile cell culture inserts (0.4 μm pore size, PET membrane, sterile, packed in 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA) were carefully positioned on the MH agar plates, ensuring that substrate stiffness does not affect the experiment. ..

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries
    Article Snippet: .. Sterile cell culture inserts (0.4 μm pore size, PET membrane, sterile, packed in 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA) were carefully positioned on the MH agar plates, ensuring that substrate stiffness does not affect the experiment. ..

    Cell Culture:

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries.
    Article Snippet: .. A 3 μL aliquot of this bacterial suspension was carefully pipetted onto a sterile filter membrane of cell culture inserts (0.4 μm pore size, PET membrane, sterile, packed in 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA). ..

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries.
    Article Snippet: .. A 3 μL aliquot of the bacterial suspension was carefully pipetted onto permeable cell culture inserts (0.4-μm pore size, PET membrane, sterile, packed in 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA) positioned on MH agar plates in sterile polystyrene Petri dishes (100 mm× 15mm) with clear lids (FisherbrandTM, Thermo Fisher Scientific, Waltham, MA, USA). ..

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries
    Article Snippet: .. A 3 μL aliquot of the bacterial suspension was carefully pipetted onto permeable cell culture inserts (0.4-μm pore size, PET membrane, sterile, packed in 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA) positioned on MH agar plates in sterile polystyrene Petri dishes (100 mm × 15 mm) with clear lids (FisherbrandTM, Thermo Fisher Scientific, Waltham, MA, USA). ..

    Article Title: Influence of Insert Brand and Culture Method on Ciliary Activity and Epithelial Cell Types in Human Nasal Air–Liquid Interface Cell Cultures
    Article Snippet: .. Recently, Brocke et al. [ ] compared two commercially available cell culture inserts from CELLTREAT ® and Corning ® Transwell ® , finding no statistically significant differences in the cell differentiation outcomes of the resulting hNECs grown at the ALI. .. In this study, we conducted a comparative analysis of primary hNEC cultures from nineteen healthy non-smoking adult donors, cultivated at the ALI using three distinct commercially available culture inserts: Corning ® Transwell ® , CELLTREAT ® , and ThinCert ® .

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries
    Article Snippet: .. A 3 μL aliquot of this bacterial suspension was carefully pipetted onto a sterile filter membrane of cell culture inserts (0.4 μm pore size, PET membrane, sterile, packed in 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA). ..

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries.
    Article Snippet: .. Sterile cell culture inserts (0.4 μm pore size, PET membrane, sterile, packed in 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA) were carefully positioned on the MH agar plates, ensuring that substrate stiffness does not affect the experiment. ..

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries
    Article Snippet: .. Sterile cell culture inserts (0.4 μm pore size, PET membrane, sterile, packed in 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA) were carefully positioned on the MH agar plates, ensuring that substrate stiffness does not affect the experiment. ..

    Pore Size:

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries.
    Article Snippet: .. A 3 μL aliquot of this bacterial suspension was carefully pipetted onto a sterile filter membrane of cell culture inserts (0.4 μm pore size, PET membrane, sterile, packed in 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA). ..

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries.
    Article Snippet: .. A 3 μL aliquot of the bacterial suspension was carefully pipetted onto permeable cell culture inserts (0.4-μm pore size, PET membrane, sterile, packed in 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA) positioned on MH agar plates in sterile polystyrene Petri dishes (100 mm× 15mm) with clear lids (FisherbrandTM, Thermo Fisher Scientific, Waltham, MA, USA). ..

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries
    Article Snippet: .. A 3 μL aliquot of the bacterial suspension was carefully pipetted onto permeable cell culture inserts (0.4-μm pore size, PET membrane, sterile, packed in 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA) positioned on MH agar plates in sterile polystyrene Petri dishes (100 mm × 15 mm) with clear lids (FisherbrandTM, Thermo Fisher Scientific, Waltham, MA, USA). ..

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries
    Article Snippet: .. A 3 μL aliquot of this bacterial suspension was carefully pipetted onto a sterile filter membrane of cell culture inserts (0.4 μm pore size, PET membrane, sterile, packed in 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA). ..

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries.
    Article Snippet: .. Sterile cell culture inserts (0.4 μm pore size, PET membrane, sterile, packed in 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA) were carefully positioned on the MH agar plates, ensuring that substrate stiffness does not affect the experiment. ..

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries
    Article Snippet: .. Sterile cell culture inserts (0.4 μm pore size, PET membrane, sterile, packed in 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA) were carefully positioned on the MH agar plates, ensuring that substrate stiffness does not affect the experiment. ..

    Positron Emission Tomography:

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries.
    Article Snippet: .. A 3 μL aliquot of this bacterial suspension was carefully pipetted onto a sterile filter membrane of cell culture inserts (0.4 μm pore size, PET membrane, sterile, packed in 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA). ..

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries.
    Article Snippet: .. A 3 μL aliquot of the bacterial suspension was carefully pipetted onto permeable cell culture inserts (0.4-μm pore size, PET membrane, sterile, packed in 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA) positioned on MH agar plates in sterile polystyrene Petri dishes (100 mm× 15mm) with clear lids (FisherbrandTM, Thermo Fisher Scientific, Waltham, MA, USA). ..

    Article Title: Temporal variation in markers of immune signaling and wound repair in primary human airway epithelial cells following acute vs. repeated woodsmoke condensate exposure.
    Article Snippet: Basal cells were cultured in PneumaCult-Ex Plus media (STEMCELL Technologies 05040) supplemented with hydrocortisone (STEMCELL Technologies #07925) and penicillin/streptomycin (Thermo Fisher 15140122). .. Upon reaching approximately 80% confluency, basal cells were passaged onto 12-well permeable inserts (0.4 μm pore, sizepolyethylene terephthalate (PET), CELLTREAT Scientific Products 230621) coated with human collagen type IV (Sigma C-7521) at a density of 200,000 cells per well and expanded in PneumaCult-Ex Plus until confluent. .. Once basal cells reached confluence on inserts, apical media was removed, and basolateral media was replaced with Pneumacult-ALI media (STEMCELL Technologies 05001) supplemented with hydrocortisone, heparin (STEMCELL Technologies 07980), and penicillin/streptomycin.

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries
    Article Snippet: .. A 3 μL aliquot of the bacterial suspension was carefully pipetted onto permeable cell culture inserts (0.4-μm pore size, PET membrane, sterile, packed in 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA) positioned on MH agar plates in sterile polystyrene Petri dishes (100 mm × 15 mm) with clear lids (FisherbrandTM, Thermo Fisher Scientific, Waltham, MA, USA). ..

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries
    Article Snippet: .. A 3 μL aliquot of this bacterial suspension was carefully pipetted onto a sterile filter membrane of cell culture inserts (0.4 μm pore size, PET membrane, sterile, packed in 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA). ..

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries.
    Article Snippet: .. Sterile cell culture inserts (0.4 μm pore size, PET membrane, sterile, packed in 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA) were carefully positioned on the MH agar plates, ensuring that substrate stiffness does not affect the experiment. ..

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries
    Article Snippet: .. Sterile cell culture inserts (0.4 μm pore size, PET membrane, sterile, packed in 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA) were carefully positioned on the MH agar plates, ensuring that substrate stiffness does not affect the experiment. ..

    Cell Differentiation:

    Article Title: Influence of Insert Brand and Culture Method on Ciliary Activity and Epithelial Cell Types in Human Nasal Air–Liquid Interface Cell Cultures
    Article Snippet: .. Recently, Brocke et al. [ ] compared two commercially available cell culture inserts from CELLTREAT ® and Corning ® Transwell ® , finding no statistically significant differences in the cell differentiation outcomes of the resulting hNECs grown at the ALI. .. In this study, we conducted a comparative analysis of primary hNEC cultures from nineteen healthy non-smoking adult donors, cultivated at the ALI using three distinct commercially available culture inserts: Corning ® Transwell ® , CELLTREAT ® , and ThinCert ® .



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    Image Search Results


    Direct contact with S. aureus biofilm is critical to induce leukocyte cytotoxicity. Primary Mφs, G-MDSCs, and PMNs were co-cultured with S. aureus biofilm either directly or separated by Transwell inserts for the indicated intervals. Leukocytes were stained with anti-CD45, Zombie NIR (viability), Apotracker Green (phosphatidylserine), and MitoSOX Red (mtROS) to quantify ( A ) leukocyte viability, ( B ) early apoptosis, ( C ) late apoptosis/necrosis, and mtROS levels in ( D ) live and ( E ) early apoptotic cells. Unstimulated leukocytes were incubated in medium for 30 min or 2 h, as indicated ( n = 9 from three independent experiments; *, P < 0.05; **, P < 0.01; ****, P < 0.0001; one-way ANOVA with Dunnett’s multiple correction between cell types).

    Journal: Infection and Immunity

    Article Title: Differential sensitivity of leukocyte populations to Staphylococcus aureus biofilm

    doi: 10.1128/iai.00654-25

    Figure Lengend Snippet: Direct contact with S. aureus biofilm is critical to induce leukocyte cytotoxicity. Primary Mφs, G-MDSCs, and PMNs were co-cultured with S. aureus biofilm either directly or separated by Transwell inserts for the indicated intervals. Leukocytes were stained with anti-CD45, Zombie NIR (viability), Apotracker Green (phosphatidylserine), and MitoSOX Red (mtROS) to quantify ( A ) leukocyte viability, ( B ) early apoptosis, ( C ) late apoptosis/necrosis, and mtROS levels in ( D ) live and ( E ) early apoptotic cells. Unstimulated leukocytes were incubated in medium for 30 min or 2 h, as indicated ( n = 9 from three independent experiments; *, P < 0.05; **, P < 0.01; ****, P < 0.0001; one-way ANOVA with Dunnett’s multiple correction between cell types).

    Article Snippet: On day 4 of biofilm growth, ~45% of the medium was removed and leukocytes were added at a density of 2.5 × 10 5 cells/well in fresh medium and incubated for 15 min, 30 min, 2 h, and 6 h. For Transwell co-cultures, Transwell inserts (0.4 μm; CELLTREAT #230635) were placed in 24-well plates above the biofilm on day 4 of growth, whereupon 7.5 × 10 5 leukocytes were added to the upper chamber for 30 min or 2 h. Leukocytes were stained and acquired as described above for planktonic co-culture studies, and unstimulated cells were included for the duration of the co-culture period.

    Techniques: Cell Culture, Staining, Incubation