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permeabilization wash buffer  (R&D Systems)


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    Structured Review

    R&D Systems permeabilization wash buffer
    Permeabilization Wash Buffer, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/permeabilization+wash+buffer/Flow+Cytometry+Permeabilization%2FWash+Buffer+I+(1X)/pmc03585142-159-20-22
    Average 93 stars, based on 42 article reviews
    permeabilization wash buffer - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Labeling:

    Article Title: A new fluorescent probe for the visualization of progerin.
    Article Snippet: Cells were grown in Dulbecco’s Modified Eagle medium (DMEM, Invitrogen) supplemented with 10 % heat-inactivated fetal bovine serum (FBS, HyClone), 1 % L-glutamine (Invitrogen), 1 % sodium pyruvate (Invitrogen), 50 U/mL penicillin and 50 μg/mL streptomycin (Invitrogen) in a humidified atmosphere with 5 % CO2 at 37 ◦C. .. For labeling studies, cells were treated with 0.125 % trypsin (Invitrogen) and plated onto glass coverslips in 24-well tissue culture dishes at a density of 20,000 cells/well and cultured under the same conditions as above for an additional 24 to 48 h. Stock solutions of the compounds were prepared in DMSO and then diluted to the final concentration with permeabilization/wash buffer I (R&D Systems, FC005) so that DMSO content was <0.5 %. ..

    Cell Culture:

    Article Title: A new fluorescent probe for the visualization of progerin.
    Article Snippet: Cells were grown in Dulbecco’s Modified Eagle medium (DMEM, Invitrogen) supplemented with 10 % heat-inactivated fetal bovine serum (FBS, HyClone), 1 % L-glutamine (Invitrogen), 1 % sodium pyruvate (Invitrogen), 50 U/mL penicillin and 50 μg/mL streptomycin (Invitrogen) in a humidified atmosphere with 5 % CO2 at 37 ◦C. .. For labeling studies, cells were treated with 0.125 % trypsin (Invitrogen) and plated onto glass coverslips in 24-well tissue culture dishes at a density of 20,000 cells/well and cultured under the same conditions as above for an additional 24 to 48 h. Stock solutions of the compounds were prepared in DMSO and then diluted to the final concentration with permeabilization/wash buffer I (R&D Systems, FC005) so that DMSO content was <0.5 %. ..

    Concentration Assay:

    Article Title: A new fluorescent probe for the visualization of progerin.
    Article Snippet: Cells were grown in Dulbecco’s Modified Eagle medium (DMEM, Invitrogen) supplemented with 10 % heat-inactivated fetal bovine serum (FBS, HyClone), 1 % L-glutamine (Invitrogen), 1 % sodium pyruvate (Invitrogen), 50 U/mL penicillin and 50 μg/mL streptomycin (Invitrogen) in a humidified atmosphere with 5 % CO2 at 37 ◦C. .. For labeling studies, cells were treated with 0.125 % trypsin (Invitrogen) and plated onto glass coverslips in 24-well tissue culture dishes at a density of 20,000 cells/well and cultured under the same conditions as above for an additional 24 to 48 h. Stock solutions of the compounds were prepared in DMSO and then diluted to the final concentration with permeabilization/wash buffer I (R&D Systems, FC005) so that DMSO content was <0.5 %. ..

    Recombinant:

    Article Title: Reliable multiplex generation of pooled induced pluripotent stem cells.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-human TRA-1-60 Novus Biologicals Cat#NB100-730AF488; RRID:AB_10598626 Mouse monoclonal anti-human SSEA-4 Novus Biologicals Cat#NBP2-26644AF488; RRID:AB_657849 Rabbit monoclonal anti-human SOX2 Abcam Cat#ab196637; RRID:AB_2943098 Rabbit monoclonal anti-human OCT4 Abcam Cat#ab300092; RRID:AB_2687916 Mouse monoclonal IgG2a Kappa BioLegend Cat#400234; RRID:AB_2864287 Mouse monoclonal anti-human Nestin R&D Systems Cat#IC1259R; RRID:AB_442286 Mouse monoclonal anti-human GFAP Novus Biologicals Cat#NBP2-33184AF647; RRID:AB_2935766 Mouse monoclonal anti-human TuJ1 BioLegend Cat#801219; RRID:AB_2876748 Mouse monoclonal anti-human TuJ1 BioLegend Cat#801203; RRID:AB_2564757 DAPI solution BD Pharmingen Cat#564907; RRID:AB_2869624 Chemicals, peptides, and recombinant proteins DPBS Gibco Cat#14190144 Matrigel Basement Membrane Matrix Corning Cat#354234 StemFlex Basal Medium ThermoFisher Scientific Cat#A33493-01 1% Pen Strep Gibco Cat#15140122 Accutase Cell Detachment Solution BioLegend Cat#423201 Y-27632 dihydrochloride Abcam Cat#ab120129 Y-27632 dihydrochloride VWR Cat#103796-110 RPMI 1640 Medium ThermoFisher Scientific Cat#72400047 Fetal Bovine Serum ThermoFisher Scientific Cat#10438026 Penicillin-Streptomycin ThermoFisher Scientific Cat#15140122 ReproTeSR Medium Stem Cell Cat#05919 Flow Cytometry Permeabilization/Wash Buffer I (1x) R&D Systems CatFC005 Paraformaldehyde 16% solution Electron Microscopy Sciences Cat#15710 StemFlex(TM) Supplement (10X) Gibco Cat#A33492-01 DMEM/F-12, HEPES Gibco Cat#11330-032 N-2 Supplement (100X) Gibco Cat#17502048 GlutaMAX Supplement Gibco Cat#35050-061 Heparin sodium salt from porcine intestinal mucosa Sigma-Aldrich Cat#H3149-1MU Neurobasal Medium Gibco Cat#21103-049 B-27 Supplement (50X), minus vitamin A Gibco Cat#12587-010 B-27 Supplement (50X), serum free Gibco Cat#17504-044 MEM Non-Essential Amino Acids Solution (100X) Gibco Cat#11140-050 Insulin solution human Sigma Life Sciences Cat#11061-68-0 Trypsin-EDTA (0.25%), phenol red Gibco Cat#25200-056 Triton X-100 Sigma-Aldrich Cat#9036-19-5 DMSO ThermoFisher Scientific Cat#D12345 Fructose ThermoFisher Scientific Cat#161355000 Glycerol ThermoFisher Scientific Cat#A16205.AP Critical commercial assays Pluripotent Stem Cell MicroBeads Miltenyi Biotec Cat#130-095-804 LS Columns Miltenyi Biotec Cat#130-042-401 (Continued on next page) e1 Cell Reports Methods 3, 100570, September 25, 2023 ..

    Membrane:

    Article Title: Reliable multiplex generation of pooled induced pluripotent stem cells.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-human TRA-1-60 Novus Biologicals Cat#NB100-730AF488; RRID:AB_10598626 Mouse monoclonal anti-human SSEA-4 Novus Biologicals Cat#NBP2-26644AF488; RRID:AB_657849 Rabbit monoclonal anti-human SOX2 Abcam Cat#ab196637; RRID:AB_2943098 Rabbit monoclonal anti-human OCT4 Abcam Cat#ab300092; RRID:AB_2687916 Mouse monoclonal IgG2a Kappa BioLegend Cat#400234; RRID:AB_2864287 Mouse monoclonal anti-human Nestin R&D Systems Cat#IC1259R; RRID:AB_442286 Mouse monoclonal anti-human GFAP Novus Biologicals Cat#NBP2-33184AF647; RRID:AB_2935766 Mouse monoclonal anti-human TuJ1 BioLegend Cat#801219; RRID:AB_2876748 Mouse monoclonal anti-human TuJ1 BioLegend Cat#801203; RRID:AB_2564757 DAPI solution BD Pharmingen Cat#564907; RRID:AB_2869624 Chemicals, peptides, and recombinant proteins DPBS Gibco Cat#14190144 Matrigel Basement Membrane Matrix Corning Cat#354234 StemFlex Basal Medium ThermoFisher Scientific Cat#A33493-01 1% Pen Strep Gibco Cat#15140122 Accutase Cell Detachment Solution BioLegend Cat#423201 Y-27632 dihydrochloride Abcam Cat#ab120129 Y-27632 dihydrochloride VWR Cat#103796-110 RPMI 1640 Medium ThermoFisher Scientific Cat#72400047 Fetal Bovine Serum ThermoFisher Scientific Cat#10438026 Penicillin-Streptomycin ThermoFisher Scientific Cat#15140122 ReproTeSR Medium Stem Cell Cat#05919 Flow Cytometry Permeabilization/Wash Buffer I (1x) R&D Systems CatFC005 Paraformaldehyde 16% solution Electron Microscopy Sciences Cat#15710 StemFlex(TM) Supplement (10X) Gibco Cat#A33492-01 DMEM/F-12, HEPES Gibco Cat#11330-032 N-2 Supplement (100X) Gibco Cat#17502048 GlutaMAX Supplement Gibco Cat#35050-061 Heparin sodium salt from porcine intestinal mucosa Sigma-Aldrich Cat#H3149-1MU Neurobasal Medium Gibco Cat#21103-049 B-27 Supplement (50X), minus vitamin A Gibco Cat#12587-010 B-27 Supplement (50X), serum free Gibco Cat#17504-044 MEM Non-Essential Amino Acids Solution (100X) Gibco Cat#11140-050 Insulin solution human Sigma Life Sciences Cat#11061-68-0 Trypsin-EDTA (0.25%), phenol red Gibco Cat#25200-056 Triton X-100 Sigma-Aldrich Cat#9036-19-5 DMSO ThermoFisher Scientific Cat#D12345 Fructose ThermoFisher Scientific Cat#161355000 Glycerol ThermoFisher Scientific Cat#A16205.AP Critical commercial assays Pluripotent Stem Cell MicroBeads Miltenyi Biotec Cat#130-095-804 LS Columns Miltenyi Biotec Cat#130-042-401 (Continued on next page) e1 Cell Reports Methods 3, 100570, September 25, 2023 ..

    Flow Cytometry:

    Article Title: Reliable multiplex generation of pooled induced pluripotent stem cells.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-human TRA-1-60 Novus Biologicals Cat#NB100-730AF488; RRID:AB_10598626 Mouse monoclonal anti-human SSEA-4 Novus Biologicals Cat#NBP2-26644AF488; RRID:AB_657849 Rabbit monoclonal anti-human SOX2 Abcam Cat#ab196637; RRID:AB_2943098 Rabbit monoclonal anti-human OCT4 Abcam Cat#ab300092; RRID:AB_2687916 Mouse monoclonal IgG2a Kappa BioLegend Cat#400234; RRID:AB_2864287 Mouse monoclonal anti-human Nestin R&D Systems Cat#IC1259R; RRID:AB_442286 Mouse monoclonal anti-human GFAP Novus Biologicals Cat#NBP2-33184AF647; RRID:AB_2935766 Mouse monoclonal anti-human TuJ1 BioLegend Cat#801219; RRID:AB_2876748 Mouse monoclonal anti-human TuJ1 BioLegend Cat#801203; RRID:AB_2564757 DAPI solution BD Pharmingen Cat#564907; RRID:AB_2869624 Chemicals, peptides, and recombinant proteins DPBS Gibco Cat#14190144 Matrigel Basement Membrane Matrix Corning Cat#354234 StemFlex Basal Medium ThermoFisher Scientific Cat#A33493-01 1% Pen Strep Gibco Cat#15140122 Accutase Cell Detachment Solution BioLegend Cat#423201 Y-27632 dihydrochloride Abcam Cat#ab120129 Y-27632 dihydrochloride VWR Cat#103796-110 RPMI 1640 Medium ThermoFisher Scientific Cat#72400047 Fetal Bovine Serum ThermoFisher Scientific Cat#10438026 Penicillin-Streptomycin ThermoFisher Scientific Cat#15140122 ReproTeSR Medium Stem Cell Cat#05919 Flow Cytometry Permeabilization/Wash Buffer I (1x) R&D Systems CatFC005 Paraformaldehyde 16% solution Electron Microscopy Sciences Cat#15710 StemFlex(TM) Supplement (10X) Gibco Cat#A33492-01 DMEM/F-12, HEPES Gibco Cat#11330-032 N-2 Supplement (100X) Gibco Cat#17502048 GlutaMAX Supplement Gibco Cat#35050-061 Heparin sodium salt from porcine intestinal mucosa Sigma-Aldrich Cat#H3149-1MU Neurobasal Medium Gibco Cat#21103-049 B-27 Supplement (50X), minus vitamin A Gibco Cat#12587-010 B-27 Supplement (50X), serum free Gibco Cat#17504-044 MEM Non-Essential Amino Acids Solution (100X) Gibco Cat#11140-050 Insulin solution human Sigma Life Sciences Cat#11061-68-0 Trypsin-EDTA (0.25%), phenol red Gibco Cat#25200-056 Triton X-100 Sigma-Aldrich Cat#9036-19-5 DMSO ThermoFisher Scientific Cat#D12345 Fructose ThermoFisher Scientific Cat#161355000 Glycerol ThermoFisher Scientific Cat#A16205.AP Critical commercial assays Pluripotent Stem Cell MicroBeads Miltenyi Biotec Cat#130-095-804 LS Columns Miltenyi Biotec Cat#130-042-401 (Continued on next page) e1 Cell Reports Methods 3, 100570, September 25, 2023 ..

    Electron Microscopy:

    Article Title: Reliable multiplex generation of pooled induced pluripotent stem cells.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-human TRA-1-60 Novus Biologicals Cat#NB100-730AF488; RRID:AB_10598626 Mouse monoclonal anti-human SSEA-4 Novus Biologicals Cat#NBP2-26644AF488; RRID:AB_657849 Rabbit monoclonal anti-human SOX2 Abcam Cat#ab196637; RRID:AB_2943098 Rabbit monoclonal anti-human OCT4 Abcam Cat#ab300092; RRID:AB_2687916 Mouse monoclonal IgG2a Kappa BioLegend Cat#400234; RRID:AB_2864287 Mouse monoclonal anti-human Nestin R&D Systems Cat#IC1259R; RRID:AB_442286 Mouse monoclonal anti-human GFAP Novus Biologicals Cat#NBP2-33184AF647; RRID:AB_2935766 Mouse monoclonal anti-human TuJ1 BioLegend Cat#801219; RRID:AB_2876748 Mouse monoclonal anti-human TuJ1 BioLegend Cat#801203; RRID:AB_2564757 DAPI solution BD Pharmingen Cat#564907; RRID:AB_2869624 Chemicals, peptides, and recombinant proteins DPBS Gibco Cat#14190144 Matrigel Basement Membrane Matrix Corning Cat#354234 StemFlex Basal Medium ThermoFisher Scientific Cat#A33493-01 1% Pen Strep Gibco Cat#15140122 Accutase Cell Detachment Solution BioLegend Cat#423201 Y-27632 dihydrochloride Abcam Cat#ab120129 Y-27632 dihydrochloride VWR Cat#103796-110 RPMI 1640 Medium ThermoFisher Scientific Cat#72400047 Fetal Bovine Serum ThermoFisher Scientific Cat#10438026 Penicillin-Streptomycin ThermoFisher Scientific Cat#15140122 ReproTeSR Medium Stem Cell Cat#05919 Flow Cytometry Permeabilization/Wash Buffer I (1x) R&D Systems CatFC005 Paraformaldehyde 16% solution Electron Microscopy Sciences Cat#15710 StemFlex(TM) Supplement (10X) Gibco Cat#A33492-01 DMEM/F-12, HEPES Gibco Cat#11330-032 N-2 Supplement (100X) Gibco Cat#17502048 GlutaMAX Supplement Gibco Cat#35050-061 Heparin sodium salt from porcine intestinal mucosa Sigma-Aldrich Cat#H3149-1MU Neurobasal Medium Gibco Cat#21103-049 B-27 Supplement (50X), minus vitamin A Gibco Cat#12587-010 B-27 Supplement (50X), serum free Gibco Cat#17504-044 MEM Non-Essential Amino Acids Solution (100X) Gibco Cat#11140-050 Insulin solution human Sigma Life Sciences Cat#11061-68-0 Trypsin-EDTA (0.25%), phenol red Gibco Cat#25200-056 Triton X-100 Sigma-Aldrich Cat#9036-19-5 DMSO ThermoFisher Scientific Cat#D12345 Fructose ThermoFisher Scientific Cat#161355000 Glycerol ThermoFisher Scientific Cat#A16205.AP Critical commercial assays Pluripotent Stem Cell MicroBeads Miltenyi Biotec Cat#130-095-804 LS Columns Miltenyi Biotec Cat#130-042-401 (Continued on next page) e1 Cell Reports Methods 3, 100570, September 25, 2023 ..



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