saponin permeabilization buffer (Thermo Fisher)

Thermo Fisher is a verified supplier

Thermo Fisher manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Thermo Fisher saponin permeabilization buffer
Saponin Permeabilization Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/permeabilization+buffer/Saponin/pmc13124628-73-19-22
Average 97 stars, based on 1 article reviews

saponin permeabilization buffer - by Bioz Stars, 2026-09

97/100 stars

Images

Blocking Assay:

Article Title: Cryoelectron tomography of HIV-1 cell-cell transmission conjugates reveals a secluded environment for viral assembly and transfer
Article Snippet: .. After 3 hours, co-cultures were fixed with 4% PFA for 30 minutes at room temperature and permeabilized with saponin 0.1% (ThermoFisher, #J63209.AK) for 10 minutes, followed by a blocking step with 2% normal goat serum and 1% BSA for 15 minutes. .. The cells were incubated overnight with the broadly neutralizing anti-Env antibody 2G12 (BEI Resources, #ARP-1476) and with conjugated antibodies for detection of p24 (KC57-FITC Conjugate) (BEI Resources, #ARP-13450) and human CD4 (CD4-APC Conjugate) (Miltenyi #130-113-222) at 1:200 dilution in a solution of PBS, 0.05% saponin, 1% BSA and 2% normal goat serum.

Article Title: Cis and trans regulatory mechanisms of extrachromosomal DNA segregation.
Article Snippet: The following compounds were added before or during the nocodazole treatment, whose dosage and timing were indicated in the corresponding main figures: A485 (Selleck Chemicals), dBET6 (Selleck Chemicals or Cayman), AU15330 (Medchemexpress), actinomycin D (Selleck Chemicals), triptolide (Selleck Chemicals), α-amanitin (Santa Cruz Biotechnology), RNase A (New England Biolabs) and CDK9i (Medchemexpress). .. Single-cell suspensions were cross-linked with 2% formaldehyde (Sigma-Aldrich) for 10 min, quenched with glycine from respective kits for 10 min and permeabilized with 0.1% saponin (Thermo Scientific) in PBS for 10 min. After blocking with 1% FBS + 0.1% saponin for 30 min, the anti-phos-MPM2 (Sigma-Aldrich 05-368, 1:1,000) primary antibody was added to the blocking solution and incubated for 1 hr Cells were then washed 3× with PBS containing 0.1% saponin before AlexaFluor-conjugated secondary antibodies (Invitrogen) staining for 1 h. After another 3× washes, DNA was stained with 1 μg ml−1 DAPI for 5 min. .. Finally, cells were sorted using the FACSAria Fusion (BD Biosciences) for G1 (2 N DNA, p-MPM2-negative) and M phase (4 N DNA, p-MPM2-positive).

Protease Inhibitor:

Article Title: A role for the poly-asparagine repeat in the Plasmodium histone acetyltransferase, PfGCN5.
Article Snippet: .. Saponin pellets were resuspended in 0.1% Igepal buffer (150mM NaCl and 50mM Tris HCl pH 7.6) containing HaltTM protease inhibitor cocktail EDTA-free (ThermoFisher, cat. number 78425), 1mM AEBSF, 50mM sodium fluoride and 1mM sodium orthovanadate. ..

Single Cell:

Incubation:

Staining:

Immunofluorescence:

Article Title: Anle138b ameliorates pathological phenotypes in mouse and cellular models of Huntington's disease.
Article Snippet: .. For combined immunofluorescence, the sections were permeabilized with 100 μg/mL Saponin (Thermo Fisher Scientific), 3% BSA in PBS at RT, for 30min. ..

Lysis:

Article Title: Decoding skeletal diversity and complexity: A biomimetic and photochemical remodeling strategy.
Article Snippet: .. Parasites were lysates after 72 hours of culture by adding 100 μl of lysis buffer [saponin (0.12 mg/ml), 0.12% Triton X- 100, 30 mM trisHCl, and 7.5 mM EDTA] containing 5× SYBR Green I (Invitrogen, 10,000× stock). ..

Review