permeabilization buffer (Servicebio Inc)

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Servicebio Inc permeabilization buffer
Permeabilization Buffer, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/permeabilization+buffer/permeabilization/pm42123683-256-8-10
Average 86 stars, based on 1 article reviews

permeabilization buffer - by Bioz Stars, 2026-09

86/100 stars

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Blocking Assay:

Article Title: Restoring cardiolipin homeostasis mitigates cerebral ischemia-reperfusion injury by suppressing ATG5-mediated neuronal autophagy-dependent ferroptosis.
Article Snippet: .. Sections then underwent heat-induced antigen retrieval with citrate buffer (pH 6.0, Servicebio, G1202, Wuhan, China), permeabilization (0.1% Triton X-100 in PBS, Servicebio, G3068, Wuhan, China) and blocking (1% BSA with 5% goat serum in PBS, Cowin Biotech Co., Ltd, CW0130S, Jiangsu, China) sequentially. .. They were then incubated with primary antibodies overnight at 4, followed by the incubation with appropriate Alexa Fluor-488/594/647- or horseradish peroxidase (HRP)-conjugated secondary antibodies (Jackson ImmunoResearch, USA) for immunofluorescence (IF) or immunohistochemistry (IHC).

Article Title: Transcriptional regulation of Rankl by Txnip-Ecd in aging and diabetic related osteoporosis
Article Snippet: .. For further immunofluorescent staining, the tissue slices were handled in the order of deparaffinization, hydration, antigen retrieval, permeabilization, blocking, and primary antibody (RANKL, Servicebio, GB11235, dilution 1:100, Novus NB,100–56512, dilution 1:100; OPG, Servicebio, GB11151, dilution 1:50) incubation overnight at 4°C, 1 h secondary antibody (Servicebio, G25302 ; Servicebio, G21303 ) incubation and nuclear staining by DAPI finally. .. Observing and imaging the sections under a fluorescence microscope (Leica, Germany) or an optical microscope (Zeiss, Germany), and analyzing them using ImageJ software.

Article Title: HSP90AB1-Mediated Ubiquitin-Proteasome Degradation of ITGBL1 Promotes Osteosarcoma Progression by Inhibiting Endoplasmic Reticulum Stress-Induced Autophagy.
Article Snippet: .. Fixed cells were blocked with PBS containing 5% BSA (Servicebio) for 30 min, followed by permeabilization with 0.1% Triton X-100 (Servicebio) in PBS for 10 min. Then incubate the cells overnight at 4◦C with the primary antibody diluted with a blocking solution. .. After three washes with PBS, cells were stained with Alexa Fluor 488 (Abcam, USA)- or Alexa Fluor 594 (Abcam, USA)conjugated secondary antibodies at room temperature for 1 h in the dark.

Staining:

Article Title: Notch Signaling Is Associated with LED Light-Regulated Papilla Regeneration in the Sea Cucumber ( Apostichopus japonicus ).
Article Snippet: .. Staining protocol: After dewaxing, sections were permeabilized with permeabilization buffer (Servicebio, Cat. No. G1204) for 20 min and rinsed three times with 1 × PBS. .. Subsequently, 60 μL of Click-iT EdU-488 staining reaction solution (Servicebio, Cat. No. G1601) was added to each section, followed by incubation in the dark for 30 min.

Incubation:

Article Title: Celastrol modulates IRS1 expression to alleviate ovarian aging and to enhance follicular development.
Article Snippet: The terminal deoxynucleotidyl transferase dUTP Nick End Labeling (TUNEL) assay was conducted with a TUNEL apoptosis detection kit (Servicebio, Wuhan, China). .. After deparaffinization, the tissue sections were treated with proteinase K for 10 min, followed by three washes with phosphate-buffered saline (PBS; G0002, Servicebio), each lasting 5 min. Once the sections had slightly dried, a membranepermeabilizing solution (Triton X-100; G1204, Servicebio) was applied, and the slides were incubated at room temperature for 20 min. After a brief drying period, a buffer was added to cover the tissue sections at room temperature for 10 min, followed by incubation with the TUNEL reaction mixture for 1 h. Subsequently, slides were incubated with DAPI solution (G1012, Servicebio) for 10 min in the dark. .. Finally, the sections were mounted in an anti-fade mounting medium (G1401, Servicebio).

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permeabilization buffer (Servicebio Inc)

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