blocking/permeabilizing solution 10% normal donkey serum (jackson immunoresearch #017-000-121) in pbs-t (Jackson Immuno)

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Jackson Immuno blocking/permeabilizing solution 10% normal donkey serum (jackson immunoresearch #017-000-121) in pbs-t
Blocking/Permeabilizing Solution 10% Normal Donkey Serum (Jackson Immunoresearch #017 000 121) In Pbs T, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/permeabilization+blocking+solution/donkey+serum/pm40301465-395-24-19
Average 90 stars, based on 1 article reviews

blocking/permeabilizing solution 10% normal donkey serum (jackson immunoresearch #017-000-121) in pbs-t - by Bioz Stars, 2026-09

90/100 stars

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Phospho-proteomics:

Article Title: Exploratory analyses of factors related to 5-aminolevulinic acid-induced intraoperative hypotension during malignant glioma surgery via literature review.
Article Snippet: .. Immunofluorescent analyses for induction of eNOS phosphorylation After de-paraffinization and blocking with 10% donkey serum (#AB_2337258; Jackson ImmunoResearch, West Grove, PA), slices were incubated with the primary antibody (Phospho-eNOS (Ser1177) Polyclonal Antibody, #PA517917; Thermo Fisher Scientific, Waltham, MA) followed by incubation with the secondary antibody conjugated with fluorescence dye (Alexa Fluor 488-conjugated donkey anti-rabbit IgG H&L antibody, #A21206; Thermo Fisher Scientific). .. Finally, fluorescent images were acquired on a confocal fluorescence microscope system (LSM880; Carl Zeiss, Oberkochen, Germany).

Blocking Assay:

Article Title: Competing Programs Shape Cortical Sensorimotor-Association Axis Development
Article Snippet: Embedded samples were sectioned at 10 μm using a cryostat (Leica, CM3050S). .. The organoid sections were washed in PBS (3 x 5 min), then incubated in a blocking solution containing 0.5% (v/v) Triton X-100 and 10% (v/v) donkey serum (Jackson ImmunoResearch Laboratories, 017-000-121) in PBS for 2 h. Primary antibodies were added in 10% (v/v) donkey serum and applied at 4 °C overnight. ..

Article Title: Modified Intracranial Aneurysm Rupture Rat Model with Angiographic Imaging.
Article Snippet: .. After blocking with 3% donkey serum (#AB_2337258; Jackson ImmunoResearch, Baltimore, MD), slices were incubated with primary antibodies, followed by fluorescent dye-conjugated secondary antibodies (Jackson ImmunoResearch). .. Fluorescence images were acquired using a confocal fluorescence microscope (LSM880; Carl Zeiss, Gottingen, Germany).

Incubation:

Fluorescence:

other:

Article Title: Supplemental Information Development of a platform to investigate long-term potentiation in hu- man iPSC-derived neuronal networks
Article Snippet: The cells were then washed 3 times in PBS followed by permeabilization and blocking for 30 min in 0.1% Triton-X (Sigma-Aldrich) in blocking buffer (5% donkey serum (Jackson ImmunoResearch) in PBS).

Article Title: Development of a platform to investigate long-term potentiation in human iPSC-derived neuronal networks
Article Snippet: The cells were then washed 3 times in PBS followed by permeabilization and blocking for 30 min in 0.1% Triton-X (Sigma-Aldrich) in blocking buffer (5% donkey serum (Jackson ImmunoResearch) in PBS).

Article Title: Phosphocreatine rescues intestinal epithelial metabolic dysfunction related to creatine kinase loss and is protective in murine colitis.
Article Snippet: To block, 100uL of blocking buffer (10% goat serum (Jackson ImmunoResearch, 005-000-121) + 0.3% Triton X-100 in PBS) was added to apical chamber of inserts, which were then placed on top of a 50uL dot of blocking buffer on a Parafilm square, then incubated for 1 hour at room temperature.

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