perm buffer (10X Genomics)

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10X Genomics perm buffer
Perm Buffer, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/perm/buffer+perm/pm42106323-210-2-4
Average 86 stars, based on 1 article reviews

perm buffer - by Bioz Stars, 2026-09

86/100 stars

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Incubation:

Article Title: Autoimmune neuroinflammation leads to neuronal death via MIF nuclease-mediated parthanatos.
Article Snippet: .. Fix/Perm buffer (10x Genomics, cat. no. PN-2000517), 4% formaldehyde in water) and then incubated overnight at 4 °C. ..

Article Title: Transcriptomic Plasticity of Human Alveolar Macrophages Revealed by Single-Cell RNA Sequencing Following Drug Exposure: Implications for Therapeutic Development.
Article Snippet: .. Live cells were counted in a haemocytometer using trypan blue stain, then centrifuged and resuspended in 1 mL of freshly prepared fixation buffer, containing 1× concentration of 10× concentrated Fix & Perm Buffer (10x Genomics PN2000517) and 4% formaldehyde, and incubated overnight at 4 ◦C. .. Cells were centrifuged at 850× g for 5 min then the fixation buffer was aspirated off and cells were resuspended and mixed gently with 1 mL of chilled 1× concentration of 8× concentrated quenching buffer (10x Genomics PN-2000516).

Article Title: Autoimmune neuroinflammation leads to neuronal death via MIF nuclease-mediated parthanatos
Article Snippet: .. Fix/Perm buffer (10x Genomics, cat. no. PN-2000517), 4% formaldehyde in water) and then incubated overnight at 4 °C. ..

Article Title: Transcriptomic Plasticity of Human Alveolar Macrophages Revealed by Single-Cell RNA Sequencing Following Drug Exposure: Implications for Therapeutic Development
Article Snippet: .. Live cells were counted in a haemocytometer using trypan blue stain, then centrifuged and resuspended in 1 mL of freshly prepared fixation buffer, containing 1× concentration of 10× concentrated Fix & Perm Buffer (10x Genomics PN-2000517) and 4% formaldehyde, and incubated overnight at 4 °C. .. Cells were centrifuged at 850× g for 5 min then the fixation buffer was aspirated off and cells were resuspended and mixed gently with 1 mL of chilled 1× concentration of 8× concentrated quenching buffer (10x Genomics PN-2000516).

Staining:

Article Title: Transcriptomic Plasticity of Human Alveolar Macrophages Revealed by Single-Cell RNA Sequencing Following Drug Exposure: Implications for Therapeutic Development
Article Snippet: .. Live cells were counted in a haemocytometer using trypan blue stain, then centrifuged and resuspended in 1 mL of freshly prepared fixation buffer, containing 1× concentration of 10× concentrated Fix & Perm Buffer (10x Genomics PN-2000517) and 4% formaldehyde, and incubated overnight at 4 °C. .. Cells were centrifuged at 850× g for 5 min then the fixation buffer was aspirated off and cells were resuspended and mixed gently with 1 mL of chilled 1× concentration of 8× concentrated quenching buffer (10x Genomics PN-2000516).

Concentration Assay:

Article Title: Transcriptomic Plasticity of Human Alveolar Macrophages Revealed by Single-Cell RNA Sequencing Following Drug Exposure: Implications for Therapeutic Development
Article Snippet: .. Live cells were counted in a haemocytometer using trypan blue stain, then centrifuged and resuspended in 1 mL of freshly prepared fixation buffer, containing 1× concentration of 10× concentrated Fix & Perm Buffer (10x Genomics PN-2000517) and 4% formaldehyde, and incubated overnight at 4 °C. .. Cells were centrifuged at 850× g for 5 min then the fixation buffer was aspirated off and cells were resuspended and mixed gently with 1 mL of chilled 1× concentration of 8× concentrated quenching buffer (10x Genomics PN-2000516).

Hybridization:

Article Title: Catecholaminergic neurons boost fibroblast osteogenic activity in keloid.
Article Snippet: .. Fix & Perm Buffer (10X Genomics cat. PN-2000517) at 4°C for 20 h. Afterwards, the fixation was quenched, and the cells were stored at −80°C until probe hybridization. .. Chromium Fixed RNA Kit, Human Transcriptome (10X Genomics cat. PN-1000474) was used for hybridization and library preparation.

Review