percoll density gradient centrifugation (Pharmacia Upjohn LLC)

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Pharmacia Upjohn LLC percoll density gradient centrifugation
Percoll Density Gradient Centrifugation, supplied by Pharmacia Upjohn LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/percoll+solution/percoll+solution/pmc04407887-57-4-8
Average 90 stars, based on 1 article reviews

percoll density gradient centrifugation - by Bioz Stars, 2026-10

90/100 stars

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Saline:

Article Title: Periostin: A Downstream Mediator of EphB4-Induced Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells
Article Snippet: .. The marrow samples were diluted with 20 mL phosphate-buffered saline (PBS) and added over 20 mL Percoll solution (Pharmacia Corporation, USA) with a density of 1.073 g/mL in a 50 mL conical tube. ..

Article Title: Periostin Upregulates Wnt/β-Catenin Signaling to Promote the Osteogenesis of CTLA4- Modified Human Bone Marrow-Mesenchymal Stem Cells
Article Snippet: .. The bone marrow samples were diluted in 20 ml phosphate-buffered saline (PBS) and separated in Percoll solution (1.073 g/mL; Pharmacia Corporation) by density gradient centrifugation (900 g for 30 min at 20 °C). .. The resulting cells were resuspended and cultured at a density of 7.55 × 10 6 cells per 37.5-cm 2 flask in human MSC basal medium containing 10% fetal bovine serum (OriCell Human Mesenchymal Stem Cell Growth Medium, Cyagen Biosciences, Guangzhou, China) at 37 °C in 5% CO 2 .

Purification:

Article Title: Lower apoptosis rate and higher CD69 expression in neutrophils from atopic individuals.
Article Snippet: Objective: In this study we aimed to examine the kinetics of CD69 expression and the susceptibility to apoptosis, in eosinophils and neutrophils, in the presence or absence of GM-CSF.. We also addressed the question whether differences between atopic patients and healthy individuals exist in this respect.. Materials and Methods: Freshly isolated eosinophils and neutrophils from non-atopic and atopic donors were analysed by flow cytometry for Annexin/PI staining, caspase 3 activation and CD69 expression.

Article Title: Natural anti-FcepsilonRIalpha autoantibodies may interfere with diagnostic tests for autoimmune urticaria.
Article Snippet: IgG autoantibodies against the -chain of the high affinity IgE receptor are claimed to play a pathogenetic role in autoimmune urticaria.. The best methods for detection of functional autoantibodies are currently the autologous serum skin test and the basophil histamine release assay.. A simplified and feasible screening test would facilitate the diagnosis of autoimmune urticaria.

Centrifugation:

Isolation:

Article Title: 1α,25-Dihydroxyvitamin D3 Induces Neutrophil Apoptosis through the p38 MAPK Signaling Pathway in Chronic Obstructive Pulmonary Disease Patients
Article Snippet: .. Neutrophils were isolated by Percoll density gradient centrifugation (Pharmacia Corporation, US). ..

Gradient Centrifugation:

Article Title: EphB4 Promotes Osteogenesis of CTLA4-Modified Bone Marrow-Derived Mesenchymal Stem Cells Through Cross Talk with Wnt Pathway in Xenotransplantation
Article Snippet: EphB4 promotes osteogenesis of CTLA4 modified bone marrow derived mesenchymal stem cells through cross-talk with Wnt pathway in xenotransplantation Running title: CTLA4 promote osteogenesis of MSCs through EphB4 Fei Zhang 1,2 , Zehua Zhang 1,2 , Dong Sun 1,2 , Shiwu Dong 1,3 , Jianzhong Xu 1,2,* , Fei Dai 1,2,* 1 National & Regional United Engineering Laboratory of Tissue Engineering, Third Military Medical University, Chongqing, People's Republic of China 2 Department of Orthopaedics, Southwest Hospital, Third Military Medical University, Chongqing, People's Republic of China 3 Department of Biomedical Materials Science, School of Biomedical Engineering, Third Military Medical University, Chongqing, People's Republic of China *Co-corresponding authors Jianzhong Xu Email: xjzslw@163.com Fei Dai Email:david-feifei@163.com;Tel: +86(023)65340297 Department of Orthopaedics, Southwest Hospital, No.29, Gaotanyan Street, Shapingba District, Chongqing 400038, China T is su e E ng in ee ri ng P ar t A D ow nl oa de d fr om o nl in e. lie be rt pu b. co m b y N ew Y or k U ni v/ 7 21 43 37 1 on 0 7/ 06 /1 5.. F or p er so na l u se o nl y. Abbreviations: BMMSCs, bone marrow derived mesenchymal stem cells; CTLA4, cytotoxic T lymphocyte–associated antigen 4; TEB, tissue-engineered bone; DBM, demineralized bone matrix; EphB4, erythropoietin-producing hepatocyte receptors B4; BMP-2, bone morphogenetic protein 2; PCR, polymerase chain reaction; WB, western-blot; PHA, phytohaemagglutinin; PBMCs, peripheral blood mononuclear cells; Runx2, runt-related transcription factor 2; ALP, alkaline phosphatase; COL1, collagen 1; OCN, osteocalcin; p-GSK-3β, phospho-glycogen synthase kinase 3 beta; GSK-3β, glycogen synthase kinase 3 beta; IFN-γ, interferon γ; TNF-α, tumor necrosis factor α; IL-17, interleukin 17; blank control, con; empty control, emp T is su e E ng in ee ri ng P ar t A D ow nl oa de d fr om o nl in e. lie be rt pu b. co m b y N ew Y or k U ni v/ 7 21 43 37 1 on 0 7/ 06 /1 5.. F or p er so na l u se o nl y. Abstract Background: Cytotoxic T lymphocyte–associated antigen 4-Ig (CTLA4-Ig) modified bone marrow derived mesenchymal stem cells (MSCs-CTLA4) have excellent osteogenic function in xenografts, but their mechanism of action remains to be elucidated.

Sedimentation:

other:

Article Title: Brain-derived respiring mitochondria exhibit homogeneous, complete and cyclosporin-sensitive permeability transition.
Article Snippet: Percoll solution, Pharmacia: 17-0891-01, was purchased from Pharmacia & Upjohn (Bedminster, NJ, USA), and NAO and TMRM from Molecular Probes (Eugene, OR, USA).

Review