low-density percoll solution (Pharmacia Upjohn LLC)
90
Structured Review
Pharmacia Upjohn LLC
low-density percoll solution
Low Density Percoll Solution, supplied by Pharmacia Upjohn LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/percoll+solution/low+density+percoll+solution/pmc02327129-112-9-12
Average 90 stars, based on 1 article reviews
Low Density Percoll Solution, supplied by Pharmacia Upjohn LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/percoll+solution/low+density+percoll+solution/pmc02327129-112-9-12
Average 90 stars, based on 1 article reviews
low-density percoll solution - by Bioz Stars,
2026-10
90/100 stars
Images
1) Product Images from "Studies of delayed systemic effects of ultraviolet B radiation (UVR) on the induction of contact hypersensitivity, 2. Evidence that interleukin-10 from UVR-treated epidermis is the critical mediator"
Article Title: Studies of delayed systemic effects of ultraviolet B radiation (UVR) on the induction of contact hypersensitivity, 2. Evidence that interleukin-10 from UVR-treated epidermis is the critical mediator
Journal:
doi: 10.1046/j.1365-2567.2000.00934.x
Figure Legend Snippet: CH‐inducing capacity of dendritic cells obtained from lymph nodes from IL‐10‐treated mice. Panels of mice received IL‐10 (200 ng) injections intradermally into abdominal skin. Eight hours later, lymph nodes not draining the injected skin (all except axillary and inguinal) were removed. Lymphoid cell suspensions were separated on density gradients using Percoll solution 1·075 g/dl, layered over 1·035 g/dl solution and centrifuged. Interface cells were collected and placed in Petri dishes at 37° for 60 min, after which non‐adherent cells were discarded. The adherent cells were then incubated at 37° overnight and non‐adherent cells were collected as cell suspension enriched for DCs. As positive control, cells were prepared from lymph nodes of untreated mice. DC‐enriched suspensions derivatized with DNFB in vitro were injected into footpads of naive syngeneic mice (2 × 104 cells in 100 µl per mouse). Five days later CH was assayed as described in the legend to Fig. 1. Bars represent mean ear‐swelling responses ± SEM for groups of five mice each. Asterisks indicate mean responses significantly less than the positive control (P < 0·0005).
Techniques Used: Injection, Incubation, Positive Control, In Vitro
Related Articles
Injection:Article Title: Studies of delayed systemic effects of ultraviolet B radiation (UVR) on the induction of contact hypersensitivity, 2. Evidence that interleukin-10 from UVR-treated epidermis is the critical mediator Article Snippet: The cells were then separated on density fractions using low‐density Percoll solution ( Incubation:Article Title: Studies of delayed systemic effects of ultraviolet B radiation (UVR) on the induction of contact hypersensitivity, 2. Evidence that interleukin-10 from UVR-treated epidermis is the critical mediator Article Snippet: The cells were then separated on density fractions using low‐density Percoll solution ( Positive Control:Article Title: Studies of delayed systemic effects of ultraviolet B radiation (UVR) on the induction of contact hypersensitivity, 2. Evidence that interleukin-10 from UVR-treated epidermis is the critical mediator Article Snippet: The cells were then separated on density fractions using low‐density Percoll solution ( In Vitro:Article Title: Studies of delayed systemic effects of ultraviolet B radiation (UVR) on the induction of contact hypersensitivity, 2. Evidence that interleukin-10 from UVR-treated epidermis is the critical mediator Article Snippet: The cells were then separated on density fractions using low‐density Percoll solution ( |