isotonic percoll solution (Cytiva Europe)

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Cytiva Europe isotonic percoll solution
Isotonic Percoll Solution, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 99/100, based on 13061 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/percoll+solution/Percoll/pm41839286-90-14-20
Average 99 stars, based on 13061 article reviews

isotonic percoll solution - by Bioz Stars, 2026-10

99/100 stars

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Gradient Centrifugation:

Article Title: Targeted inhibition of inflammatory liver macrophages with a multifunctional nanoparticle ameliorates metabolic dysfunction-associated steatohepatitis.
Article Snippet: .. Briefly, C57BL/6 mice were perfused with EDTA (0.5 mM, MP Biomedicals LLC, 195173) and collagenase type IV (0.075%, Gibco, 17104-019), then apply gradient centrifugation with Percoll (20% and 50%, GE Healthcare, 10257134) to have higher yield. .. Isolated Kupffer cells cultured in Dulbecco's modified Eagle medium supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (Invitrogen) for following experiment.

Article Title: Single-allele nanoscale mapping of regulatory variants
Article Snippet: Brain and spinal cords were dissected and digested in HBSS supplemented with 0.05% collagenase D (Roche), 0.1 mg ml −1 of tosyl- L -lysyl-chloromethane hydrochloride (TLCK; Sigma-Aldrich) and 0.025 U ml −1 of deoxyribonuclease I (Thermo Fisher Scientific). .. A Percoll (GE Healthcare Life Sciences) gradient centrifugation at 200 g for 40 min was used to isolate mononuclear cells. ..

Centrifugation:

Article Title: CD112 Lactylation Drives Dual Immune Evasion From CD8+ T Cells and NK Cells.
Article Snippet: .. Tumor-infiltrating lymphocytes (TILs) were nriched by density-gradient centrifugation using Lymphocyte eparation Medium (17089101, Cytiva). ..

Incubation:

Article Title: Lysosomal multi-omics reveals altered sphingolipid catabolism associated with lysosomal dysfunction in the aging brain.
Article Snippet: Following transcardial perfusion with saline, cortices were removed and processed by mechanical disruption on a 70 μm filter screen and resuspended in RPMI-1640 (Quality Biological, 112–025-101).59 Ten Units (U) Papain (Sigma-Aldrich, P4762), 10 mg/mL DNase II (Sigma, 10104159001), 1 mg/mL Collagenase-Dispase (Sigma, 10269638001), and 1 μL of GolgiPlug containing brefeldin A (BD Biosciences, 555029) were added to the brain suspension and incubated on a shaker at 200 rotations per minute (RPM) for 1 h at 37◦C for mechanical and enzymatic digestion of the brain tissue. .. After incubation, leukocytes were separated from other brain cells by a Percoll gradient (GE Healthcare, Chicago, IL, USA, GE17-5445-02). .. Brain leukocytes were resuspended in 70% Percoll-HBSS and were slowly injected under a 30% Percoll-RPMI layer using a blunt popper pipetting needle (Sigma).

Blocking Assay:

Article Title: Acanthamoeba castellanii genome reannotation and multi-omic encystation profiling reveals cyst wall proteins and carbohydrate-active enzymes
Article Snippet: This study Calcofluor white Sigma Aldrich 1809 2,6-dichlorobenzonitrile Sigma D57558 Alexa Flour 488 NHS ester Thermo A20000 Alexa Flour 594 NHS ester Thermo A20004 (Continued on next page) e1 Cell Press Blue 1, 100125, November 16, 2026 Resource ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Alexa Flour 647 NHS ester Thermo A20006 Benzonase (Purity >99%) Millipore 70664–3 Percoll Cytiva 17089101 OptiPrep Sigma D1556 EveryBlot Blocking Buffer Bio-Rad 12010020 ProLong Glass Antifade Mountant with NucBlue Stain Thermo P36981 cOmplete Protease Inhibitor Cocktail Sigma/Roche 11697498001 TRI Reagent Solution Thermo AM9738 Water MilliporeSigma WX0008-1 UltraPure SDS Solution, 10% Invitrogen 15553–035 Triethylammonium bicarbonate buffer, 1 M MilliporeSigma 18597-500ML Magnesium chloride solution, BioUltra, Molecular Biology, ∼1 M in H2O Sigma-Aldrich 63069-100ML Aprotinin Sigma-Aldrich A6106-1MG Leupeptin Roche 11017101001 Phenylmethanesulfonyl fluoride solution, 0.1 M in ethanol (T) Sigma-Aldrich 93482-50ML-F Benzonase Nuclease MilliporeSigma E1014-5KU Pierce DTT (Dithiothreitol) Thermo Scientific A39255 Iodoacetamide Sigma-Aldrich A3221-10VL S-Trap Mini Column PROTIFI C02-mini-40 S-Trap Micro Column PROTIFI C02-micro-40 Phosphoric Acid LabChem LC186401 Formic Acid MilliporeSigma 5330020050 Acetonitrile J.T.Baker 9829–03 Sep-Pak tC18 96-well Plate, 40 mg Sorbent per Well Waters 186002320 Empore C18 Extraction Disks CSD Analytical 76333–132 Methanol Honeywell 34966-4X4L Trifluoroacetic acid Sigma-Aldrich 302031-10X1ML 1-Propanol Sigma-Aldrich 279544-1L Sequencing Grade Modified Trypsin Promega V5113 Lysyl EndopeptidaseR, Mass Spectrometry Grade (Lys-C) FUJIFILM Wako Pure Chemical Corporation 129–02541 Evotips Evosep Catalog #: EV2011 Evosep Endurance Column (0.15 mm × 15 cm, 1.9μm) Evosep Catalog #: EV1106 Evosep Stainless Steel Emitter (ID 30μm) Evosep Catalog #: EV1086 0.50-mL Alphanumeric Micronic Vial Rack Micronic Catalog #: MPW42072LBC3 Matrix SepraSeal Cap Mats Thermo Scientific Catalog #: 4466BLU 96-Well Microplate Flat Bottom Clear VWR 82050–760 PepSep C18 25 cm × 75 μm, 1.5 μm Bruker Daltonics 1893484 ReproSil-Pur 120 C18Aq media by Dr. Maisch GmbH ESI Source Solutions r119.aq.0001 UNIPLATE microplates Whatman 7701–5200 Critical commercial assays Pierce BCA Protein Assay Kit Thermo Fischer Scientific Cat# 23225 DC Protein Assay Kit Bio-Rad 5000112 Deposited data Acanthamoeba castellanii strain Neff ACANB genome annotation This study Provided as a Supplemental Dataset (Data S2, ACANB.gtf) (Continued on next page) Cell Press Blue 1, 100125, November 16, 2026 e2 Resource ll OPEN ACCESS ..

Glass:

Staining:

Protease Inhibitor:

Extraction:

Sequencing:

Modification:

Mass Spectrometry:

BIA-KA:

DC Protein Assay:

Isolation:

Article Title: Cytokine-mediated activation of exhausted T cells
Article Snippet: .. Lungs were enzymatically digested with collagenase D (Gibco) and DNase I (VWR) for 30 min, and immune cells were isolated using a 30% Percoll (GE Healthcare) gradient as described previously ( ). .. When staining for intracellular cytokines, cells were incubated with Brefeldin A (BioLegend) for 4 h at 37 °C prior to staining.

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