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Biochrom percoll® separating solution
Percoll® Separating Solution, supplied by Biochrom, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/percoll+solution/percoll+separating+solution/pmc04181728-118-20-21
Average 90 stars, based on 1 article reviews
percoll® separating solution - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

other:

Article Title: SIRT1 inhibits monocyte adhesion to the vascular endothelium by suppressing Mac-1 expression on monocytes.
Article Snippet: Peripheral blood mononuclear cells (PBMCs) were separated by density-gradient centrifugation using a Histopaque®-1077 (Sigma) separating solution (Biochrom, Berlin, Germany)26.

Article Title: Modulatory effects of bovine seminal plasma on uterine inflammatory processes.
Article Snippet: In this study, a simple model to simulate a uterine environment affected by subclinical endometritis was established by culturing isolated primary bovine uterine epithelial cells (pbUEC).. Co-incubation of pbUEC and polymorphonuclear (PMN) granulocytes derived from peripheral bovine blood samples, was performed before testing the cell culture supernatant for production of interleukin-8 (IL-8) via ELISA.. Cytokine secretion was only detectable after co-incubation of pbUEC with PMN, whereas neither pbUEC nor PMN alone generated IL-8 in relevant chemo attractive doses.

Article Title: Increased of exhaled breath condensate neutrophil chemotaxis in acute exacerbation of COPD
Article Snippet: The leukocytes-rich liquid phase was diluted three times with phosphate buffer saline (PBS) and was centrifuged through a Percoll® separating solution (Biochrom- AG- Germany) gradient density 1.077 (centrifugation at 400 g for 30 minutes at 22°C) to separate the mononuclear leukocytes from the granulocyte fraction (>95% neutrophils).

Article Title: Defective eosinophil chemotaxis to eotaxin in a patient with chronic lower baseline CD4+ T-lymphocytes and elevated CD8+ T cells
Article Snippet: Reagents The following reagents were used: eotaxin (R&D Systems, Minneapolis, MN, USA), ammonium chloride solution (Acros Organics, Morris Plains, NJ, USA), and Percoll separating solution with a density of 1.124 g/ml (Biochrom AG, Berlin, Germany).

Isolation:

Article Title: NK cells control breast cancer and related cancer stem cell hematological spread
Article Snippet: NK cells were cultured overnight in RPMI 10% FBS and 1% Penicillin/Streptomycin, either with or without 200 U/ml IL-2 (Peprotech). .. For human NK cells, blood was obtained from healthy donors and peripheral blood lymphocytes were isolated by Biocoll separating solution (Biochrom AG) density gradient centrifugation. .. Enriched NK cells were isolated from the separated PBMCs using the NK cell isolation kit (Miltenyi Biotec) according to manufacturer’s instructions.

Gradient Centrifugation:

Article Title: NK cells control breast cancer and related cancer stem cell hematological spread
Article Snippet: NK cells were cultured overnight in RPMI 10% FBS and 1% Penicillin/Streptomycin, either with or without 200 U/ml IL-2 (Peprotech). .. For human NK cells, blood was obtained from healthy donors and peripheral blood lymphocytes were isolated by Biocoll separating solution (Biochrom AG) density gradient centrifugation. .. Enriched NK cells were isolated from the separated PBMCs using the NK cell isolation kit (Miltenyi Biotec) according to manufacturer’s instructions.



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