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percoll plus solution 60  (Cytiva Europe)


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    Cytiva Europe percoll plus solution 60
    Percoll Plus Solution 60, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/percoll+plus+solution/percoll+solution/pm36040029-271-2-15
    Average 90 stars, based on 1 article reviews
    percoll plus solution 60 - by Bioz Stars, 2026-10
    90/100 stars

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    other:

    Article Title: Targeting thrombospondin-2 retards liver fibrosis by inhibiting TLR4-FAK/TGF-β signaling
    Article Snippet: Liver parenchyma cells in the filtrate were removed 6 / 42 using 30% Percoll (Cytiva, Upsala, Sweden) solution (dissolved in Hanks’ Balanced Salt Solution (HBSS) without Ca2+, Mg2+, and phenol red [Solarbio]).

    Gentle:

    Article Title: Isolation and characterization of synaptic structures from human neural organoids
    Article Snippet: .. IMR90 clone 4 (WiCell #iPS(IMR90)-4) Matrigel® Growth factor reduced (GFR) (Corning #356230) mTESR1 medium (Stem Cell Technologies #85870) Gentle cell dissociation reagent (Stem Cell Technologies #07174) STEMdiff Dorsal Forebrain Organoid Differentiation Kit (Stem Cell Technologies #08620) AggrewellTM800 24-well plates (Stem Cell Technologies #34815) ROCK inhibitor, Y-27632 (Sigma-Aldrich, #SCM075) Penicillin/Streptomycin (Gibco #15140) Amphotericin B (Thermo Scientific #15290026) H9 ESCs (WiCell, WA09) StemFlex medium (Thermo Fisher Scientific, #A3349401) Matrigel® (Corning, #356234) STEMdiff Cerebral Organoid Kit (StemCell Technologies, #08570) Ethylenediamine tetraacetic acid (Merck, #1.08418.1000) Millicell Cell Culture Insert (Millipore, #PICMORG50) Neurobasal (Thermo Fisher Scientific, #21103049) B-27 supplement (Thermo Fisher Scientific, #17504044) Glutamax (Thermo Fisher Scientific, #35050038), Antibiotic-antimycotic (Thermo Fisher Scientific, #15240062) Amphotericin B solution (Merck, #A2942-20ML) D-(+)-Glucose (Sigma, #G7021) Sucrose (Sigma, #84097) Trizma base (Sigma #93352) Dithiothreitol (DTT, Sigma #D9163) Tissue grinder (15-ml Potter-Elvehjem type Teflon-glass tissue grinder, Wheaton, 358009) PercollTM solution (Cytiva, #17089102) KCl (Sigma, #P9541) NaCl (VWR, #27810.295) HEPES (Sigma, #H3375) MgSO 4 (Merck, # 7487-88-9) CaCl 2 anhydrous (Merck, #2366663) Na 2 HPO 4 · 2H 2 O (Merck, #1.06580.0500) Sodium Pyruvate (Gibco, #11360) NaHCO 3 anhydrous (Sigma, #71345) 10 kDa spin filters, Ultra-0.5 Centrifugal Filter Unit (Amicon, #UFC5010) TEAB, Triethylammonium bicarbonate (Sigma #T7408) Sodium deoxycholate (SDS, Sigma #D6750) SafeSeal Microcentrifuge Tubes, Low-binding (Sorenson BioScience Inc.) NanoPhotometer N60/N50 (Implen) Iodoacetamide (Sigma #I1149) Methylated trypsin (made in-house) [ ] LysC, Lysyl Endopeptidase (Wako, #129-02541) Formic acid (Merck #1.11670.0250) Pierce Quantitative Fluorometric Peptide Assay (Thermo, #23290) Aurora Elite column 15 cm x 75 μm, C18 1.6 m (ESI Source Solutions) Acetonitrile (VWR #83640.320) TMTpro 16-plex (Thermo Fisher Scientific #A44520) TMTpro 18-plex, TMTpro-134C & TMTpro-135N Label Reagents (Thermo Fisher Scientific #A52046) Ammonium Formate (Riedel-de Haën #25204) Trifluoroacetic acid (Merck #1.08178.0050) Glycolic acid (Sigma #124737) Ammonium hydroxide solution (Merck #1.05428.0500) Titanium dioxide beads, 5 μm (GL Science #5020-75010) Oasis HLB column (Waters #186000132) Methanol (VWR #20864.320) Reprosil-Pur 120 C18-AQ, 5 μm (Dr. Maisch GmbH, #r15.aq.) .. Reprosil-Pur 120 C18-AQ, 3 μm (Dr. Maisch GmbH, # r13.aq.)

    Cell Culture:

    Article Title: Isolation and characterization of synaptic structures from human neural organoids
    Article Snippet: .. IMR90 clone 4 (WiCell #iPS(IMR90)-4) Matrigel® Growth factor reduced (GFR) (Corning #356230) mTESR1 medium (Stem Cell Technologies #85870) Gentle cell dissociation reagent (Stem Cell Technologies #07174) STEMdiff Dorsal Forebrain Organoid Differentiation Kit (Stem Cell Technologies #08620) AggrewellTM800 24-well plates (Stem Cell Technologies #34815) ROCK inhibitor, Y-27632 (Sigma-Aldrich, #SCM075) Penicillin/Streptomycin (Gibco #15140) Amphotericin B (Thermo Scientific #15290026) H9 ESCs (WiCell, WA09) StemFlex medium (Thermo Fisher Scientific, #A3349401) Matrigel® (Corning, #356234) STEMdiff Cerebral Organoid Kit (StemCell Technologies, #08570) Ethylenediamine tetraacetic acid (Merck, #1.08418.1000) Millicell Cell Culture Insert (Millipore, #PICMORG50) Neurobasal (Thermo Fisher Scientific, #21103049) B-27 supplement (Thermo Fisher Scientific, #17504044) Glutamax (Thermo Fisher Scientific, #35050038), Antibiotic-antimycotic (Thermo Fisher Scientific, #15240062) Amphotericin B solution (Merck, #A2942-20ML) D-(+)-Glucose (Sigma, #G7021) Sucrose (Sigma, #84097) Trizma base (Sigma #93352) Dithiothreitol (DTT, Sigma #D9163) Tissue grinder (15-ml Potter-Elvehjem type Teflon-glass tissue grinder, Wheaton, 358009) PercollTM solution (Cytiva, #17089102) KCl (Sigma, #P9541) NaCl (VWR, #27810.295) HEPES (Sigma, #H3375) MgSO 4 (Merck, # 7487-88-9) CaCl 2 anhydrous (Merck, #2366663) Na 2 HPO 4 · 2H 2 O (Merck, #1.06580.0500) Sodium Pyruvate (Gibco, #11360) NaHCO 3 anhydrous (Sigma, #71345) 10 kDa spin filters, Ultra-0.5 Centrifugal Filter Unit (Amicon, #UFC5010) TEAB, Triethylammonium bicarbonate (Sigma #T7408) Sodium deoxycholate (SDS, Sigma #D6750) SafeSeal Microcentrifuge Tubes, Low-binding (Sorenson BioScience Inc.) NanoPhotometer N60/N50 (Implen) Iodoacetamide (Sigma #I1149) Methylated trypsin (made in-house) [ ] LysC, Lysyl Endopeptidase (Wako, #129-02541) Formic acid (Merck #1.11670.0250) Pierce Quantitative Fluorometric Peptide Assay (Thermo, #23290) Aurora Elite column 15 cm x 75 μm, C18 1.6 m (ESI Source Solutions) Acetonitrile (VWR #83640.320) TMTpro 16-plex (Thermo Fisher Scientific #A44520) TMTpro 18-plex, TMTpro-134C & TMTpro-135N Label Reagents (Thermo Fisher Scientific #A52046) Ammonium Formate (Riedel-de Haën #25204) Trifluoroacetic acid (Merck #1.08178.0050) Glycolic acid (Sigma #124737) Ammonium hydroxide solution (Merck #1.05428.0500) Titanium dioxide beads, 5 μm (GL Science #5020-75010) Oasis HLB column (Waters #186000132) Methanol (VWR #20864.320) Reprosil-Pur 120 C18-AQ, 5 μm (Dr. Maisch GmbH, #r15.aq.) .. Reprosil-Pur 120 C18-AQ, 3 μm (Dr. Maisch GmbH, # r13.aq.)

    Methylation:

    Article Title: Isolation and characterization of synaptic structures from human neural organoids
    Article Snippet: .. IMR90 clone 4 (WiCell #iPS(IMR90)-4) Matrigel® Growth factor reduced (GFR) (Corning #356230) mTESR1 medium (Stem Cell Technologies #85870) Gentle cell dissociation reagent (Stem Cell Technologies #07174) STEMdiff Dorsal Forebrain Organoid Differentiation Kit (Stem Cell Technologies #08620) AggrewellTM800 24-well plates (Stem Cell Technologies #34815) ROCK inhibitor, Y-27632 (Sigma-Aldrich, #SCM075) Penicillin/Streptomycin (Gibco #15140) Amphotericin B (Thermo Scientific #15290026) H9 ESCs (WiCell, WA09) StemFlex medium (Thermo Fisher Scientific, #A3349401) Matrigel® (Corning, #356234) STEMdiff Cerebral Organoid Kit (StemCell Technologies, #08570) Ethylenediamine tetraacetic acid (Merck, #1.08418.1000) Millicell Cell Culture Insert (Millipore, #PICMORG50) Neurobasal (Thermo Fisher Scientific, #21103049) B-27 supplement (Thermo Fisher Scientific, #17504044) Glutamax (Thermo Fisher Scientific, #35050038), Antibiotic-antimycotic (Thermo Fisher Scientific, #15240062) Amphotericin B solution (Merck, #A2942-20ML) D-(+)-Glucose (Sigma, #G7021) Sucrose (Sigma, #84097) Trizma base (Sigma #93352) Dithiothreitol (DTT, Sigma #D9163) Tissue grinder (15-ml Potter-Elvehjem type Teflon-glass tissue grinder, Wheaton, 358009) PercollTM solution (Cytiva, #17089102) KCl (Sigma, #P9541) NaCl (VWR, #27810.295) HEPES (Sigma, #H3375) MgSO 4 (Merck, # 7487-88-9) CaCl 2 anhydrous (Merck, #2366663) Na 2 HPO 4 · 2H 2 O (Merck, #1.06580.0500) Sodium Pyruvate (Gibco, #11360) NaHCO 3 anhydrous (Sigma, #71345) 10 kDa spin filters, Ultra-0.5 Centrifugal Filter Unit (Amicon, #UFC5010) TEAB, Triethylammonium bicarbonate (Sigma #T7408) Sodium deoxycholate (SDS, Sigma #D6750) SafeSeal Microcentrifuge Tubes, Low-binding (Sorenson BioScience Inc.) NanoPhotometer N60/N50 (Implen) Iodoacetamide (Sigma #I1149) Methylated trypsin (made in-house) [ ] LysC, Lysyl Endopeptidase (Wako, #129-02541) Formic acid (Merck #1.11670.0250) Pierce Quantitative Fluorometric Peptide Assay (Thermo, #23290) Aurora Elite column 15 cm x 75 μm, C18 1.6 m (ESI Source Solutions) Acetonitrile (VWR #83640.320) TMTpro 16-plex (Thermo Fisher Scientific #A44520) TMTpro 18-plex, TMTpro-134C & TMTpro-135N Label Reagents (Thermo Fisher Scientific #A52046) Ammonium Formate (Riedel-de Haën #25204) Trifluoroacetic acid (Merck #1.08178.0050) Glycolic acid (Sigma #124737) Ammonium hydroxide solution (Merck #1.05428.0500) Titanium dioxide beads, 5 μm (GL Science #5020-75010) Oasis HLB column (Waters #186000132) Methanol (VWR #20864.320) Reprosil-Pur 120 C18-AQ, 5 μm (Dr. Maisch GmbH, #r15.aq.) .. Reprosil-Pur 120 C18-AQ, 3 μm (Dr. Maisch GmbH, # r13.aq.)

    Titanium Dioxide:

    Article Title: Isolation and characterization of synaptic structures from human neural organoids
    Article Snippet: .. IMR90 clone 4 (WiCell #iPS(IMR90)-4) Matrigel® Growth factor reduced (GFR) (Corning #356230) mTESR1 medium (Stem Cell Technologies #85870) Gentle cell dissociation reagent (Stem Cell Technologies #07174) STEMdiff Dorsal Forebrain Organoid Differentiation Kit (Stem Cell Technologies #08620) AggrewellTM800 24-well plates (Stem Cell Technologies #34815) ROCK inhibitor, Y-27632 (Sigma-Aldrich, #SCM075) Penicillin/Streptomycin (Gibco #15140) Amphotericin B (Thermo Scientific #15290026) H9 ESCs (WiCell, WA09) StemFlex medium (Thermo Fisher Scientific, #A3349401) Matrigel® (Corning, #356234) STEMdiff Cerebral Organoid Kit (StemCell Technologies, #08570) Ethylenediamine tetraacetic acid (Merck, #1.08418.1000) Millicell Cell Culture Insert (Millipore, #PICMORG50) Neurobasal (Thermo Fisher Scientific, #21103049) B-27 supplement (Thermo Fisher Scientific, #17504044) Glutamax (Thermo Fisher Scientific, #35050038), Antibiotic-antimycotic (Thermo Fisher Scientific, #15240062) Amphotericin B solution (Merck, #A2942-20ML) D-(+)-Glucose (Sigma, #G7021) Sucrose (Sigma, #84097) Trizma base (Sigma #93352) Dithiothreitol (DTT, Sigma #D9163) Tissue grinder (15-ml Potter-Elvehjem type Teflon-glass tissue grinder, Wheaton, 358009) PercollTM solution (Cytiva, #17089102) KCl (Sigma, #P9541) NaCl (VWR, #27810.295) HEPES (Sigma, #H3375) MgSO 4 (Merck, # 7487-88-9) CaCl 2 anhydrous (Merck, #2366663) Na 2 HPO 4 · 2H 2 O (Merck, #1.06580.0500) Sodium Pyruvate (Gibco, #11360) NaHCO 3 anhydrous (Sigma, #71345) 10 kDa spin filters, Ultra-0.5 Centrifugal Filter Unit (Amicon, #UFC5010) TEAB, Triethylammonium bicarbonate (Sigma #T7408) Sodium deoxycholate (SDS, Sigma #D6750) SafeSeal Microcentrifuge Tubes, Low-binding (Sorenson BioScience Inc.) NanoPhotometer N60/N50 (Implen) Iodoacetamide (Sigma #I1149) Methylated trypsin (made in-house) [ ] LysC, Lysyl Endopeptidase (Wako, #129-02541) Formic acid (Merck #1.11670.0250) Pierce Quantitative Fluorometric Peptide Assay (Thermo, #23290) Aurora Elite column 15 cm x 75 μm, C18 1.6 m (ESI Source Solutions) Acetonitrile (VWR #83640.320) TMTpro 16-plex (Thermo Fisher Scientific #A44520) TMTpro 18-plex, TMTpro-134C & TMTpro-135N Label Reagents (Thermo Fisher Scientific #A52046) Ammonium Formate (Riedel-de Haën #25204) Trifluoroacetic acid (Merck #1.08178.0050) Glycolic acid (Sigma #124737) Ammonium hydroxide solution (Merck #1.05428.0500) Titanium dioxide beads, 5 μm (GL Science #5020-75010) Oasis HLB column (Waters #186000132) Methanol (VWR #20864.320) Reprosil-Pur 120 C18-AQ, 5 μm (Dr. Maisch GmbH, #r15.aq.) .. Reprosil-Pur 120 C18-AQ, 3 μm (Dr. Maisch GmbH, # r13.aq.)

    Reverse Transcription:

    Article Title: Intestinal-region-specific functions of AHR in intrinsic enteric neurons during infections.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit anti-HuC/D (EPR19098), Alexa Fluor 488 Abcam Cat# ab237234 Rabbit anti-HuC/D (EPR19098), Alexa Fluor 647 Abcam Cat# ab237235 AffiniPureTM Donkey Anti-Mouse IgG (H + L), CyTM5 Jackson ImmunoResearch Cat# 715-175-151; RRID: AB_2340820 AffiniPureTM Donkey Anti-Rabbit IgG (H + L), CyTM5 Jackson ImmunoResearch Cat# 711-175-152; RRID: AB_2340607 AffiniPureTM Donkey Anti-Rabbit IgG (H + L), CyTM3 Jackson ImmunoResearch Cat# 711-165-152; RRID: AB_2307443 mouse anti-HuC/D (16A11) Thermo Fisher Scientific Cat# A-21271; RRID: AB_221448 TotalSeqTM-B0451 anti-Nuclear Pore Complex Proteins Hashtag 1 Antibody Biolegend Cat# 682235; RRID:AB_2892484 TotalSeqTM-B0452 anti-Nuclear Pore Complex Proteins Hashtag 1 Antibody Biolegend Cat# 682237; RRID:AB_2892485 TotalSeqTM-B0457 anti-Nuclear Pore Complex Proteins Hashtag 1 Antibody Biolegend Cat# 682241; RRID:AB_2892487 TotalSeqTM-B0460 anti-Nuclear Pore Complex Proteins Hashtag 10 Antibody Biolegend Cat# 682249; RRID:AB_2936637 TotalSeqTM-B0461 anti-Nuclear Pore Complex Proteins Hashtag 11 Antibody Biolegend Cat# 682251; RRID:AB_2936639 TotalSeqTM-B0462 anti-Nuclear Pore Complex Proteins Hashtag 12 Antibody Biolegend Cat# 682253; RRID:AB_2936636 TotalSeqTM-B0463 anti-Nuclear Pore Complex Proteins Hashtag 13 Antibody Biolegend Cat# 682255; RRID:AB_2936630 TotalSeqTM-B0464 anti-Nuclear Pore Complex Proteins Hashtag 14 Antibody Biolegend Cat# 682245; RRID:AB_2936618 TotalSeqTM-B0301 anti-mouse Hashtag 1 Antibody Biolegend Cat# 155831; RRID:AB_2814067 TotalSeqTM-B0302 anti-mouse Hashtag 2 Antibody Biolegend Cat# 155833; RRID:AB_2814068 TotalSeqTM-B0303 anti-mouse Hashtag 3 Antibody Biolegend Cat# 155835; RRID:AB_2814069 TotalSeqTM-B0304 anti-mouse Hashtag 4 Antibody Biolegend Cat# 155837; RRID:AB_2814070 TotalSeqTM-B0305 anti-mouse Hashtag 5 Antibody Biolegend Cat# 155839; RRID:AB_2814071 TotalSeqTM-B0306 anti-mouse Hashtag 6 Antibody Biolegend Cat# 155841; RRID:AB_2814072 TotalSeqTM-B0307 anti-mouse Hashtag 7 Antibody Biolegend Cat# 155843; RRID:AB_2814073 TotalSeqTM-B0308 anti-mouse Hashtag 8 Antibody Biolegend Cat# 155845; RRID:AB_2814074 Bacterial and virus strains C. rodentium strain (DBS100) Dr. Xiaohuan Guo, Tsinghua Univeristy N/A C. albicans strain (SC5314) Dr. Guanghua Huang, Fudan Univeristy N/A Chemicals, peptides, and recombinant proteins DAPI Sigma-Aldrich Cat# D9542 Fluoromount-G SouthernBiotech Cat#0100-01 OCT SAKURA Cat# H22135 Fixable Viability Dye eFluor 780 Thermo Fisher Scientific Cat# 65-0865-14 (Continued on next page) Cell Reports 44, 115524, April 22, 2025 11 .. REAGENT or RESOURCE SOURCE IDENTIFIER Collagenase D Roche Cat# 11088882001 DNase I Roche Cat# 10104159001 RPMI-1640 Thermo Fisher Scientific Cat# 72400-047 Percoll Plus GE(cytiva) Cat# 17089109-1 Fixation/Permeabilization Solution Kit BD Biosciences Cat#554714 FBS CellMax Cat# SA211.02 DTT,DL-DITHIOTHREITOL Sigma Cat# D9779 Triton X-100 Sigma-Aldrich Cat# T8787 Normal Donkey Serum Jackson ImmunoResearch Cat# 017-000-001; RRID: AB_2337254 UltraPure DNase/RNase-Free Distilled Water Thermo Cat# 10977015 Trypan Blue solution Sigma Cat# T8154 Tween 20 Sigma-Aldrich Cat#P9416 UltraPureTM 1 M Tris-HCI Thermo Fisher Scientific Cat#15567027 5M NaCl RNase Free Thermo Fisher Scientific Cat#AM9760G MgCl2 Sigma-Aldrich Cat#M1028 CaCl2 Sigma-Aldrich Cat#21115 Bovine Serum Albumin Sigma-Aldrich Cat#A1933 Triton X-100 Diamond Cat#A110694-0100 TCL buffer QIAGEN Cat# 1031576 2-Mercaptoethanol Sigma-Aldrich Cat# M3148-25ML iScript Reverse Transcription Supermix for RT-qPCR Bio-Rad Cat# 1708841 iTaqTM Universal SYBR Green Supermix Bio-Rad Cat# 1725121 DSS MPbio Cat# 216011080 Carmine red dye Aladdin Cat# C305814 Methylcellulose Sigma-Aldrich Cat# M0512 Evans blue dye Sigma-Aldrich Cat# E2129 AIN-76A or AIN-76A supplemented with I3C (200 mg/kg) SYSEBIO Cat# PD23040401 Streptomycin sulfate Sangon Biotech A100382 Fluconazole Sigma-Aldrich PHR1160 Gentamycin sulfate Sangon Biotech A506614 Vancomycin HCl Harvey V31515 Ampicillin sodium Sangon Biotech A610028 Critical commercial assays 30 Feature Barcode Kit 10X Genomics Cat#PN-1000262 Chromium Next GEM Single Cell 30 Kit v3.1 10X Genomics Cat#PN-1000268 Dual index Kit TT Set A 10X Genomics Cat#PN-1000215 Dual index Kit NT Set A 10X Genomics Cat#PN-1000242 Deposited Data snRNA-seq and scRNA-seq This paper GEO: GSE290795 Experimental models: Organisms/strains Mouse: C57BL/6J Jackson Laboratory JAX:000664; RRID:IMSR_JAX:000664 Mouse: Tg(Actl6b-Cre)4092Jiwu/J Jackson Laboratory JAX:027826; RRID:IMSR_JAX:027826 Mouse: B6; 129-Gt(ROSA) 26Sortm5(CAG Sun1/sfGFP)Nat/J Jackson Laboratory JAX:021039; RRID:IMSR_JAX:021039 (Continued on next page) 12 Cell Reports 44, 115524, April 22, 2025 .. C56BL/6J (Jax 000664), Actl6b-Cre (Tg(Actl6b-Cre)4092Jiwu/J, Jax 027826), Ahrflox/flox (Ahrtm3.1Bra/J, Jax 006203), Sun1-GFP (B6; 129-Gt(ROSA)26Sortm5(CAG-Sun1/sfGFP)Nat/J, Jax 21039) and Syn-Cre (B6.Cg-Tg(Syn1-cre)671Jxm/J, Jax 003966) were originally obtained from the Jackson Laboratory.

    Article Title: Fate mapping of Spp1 expression reveals age-dependent plasticity of disease-associated microglia-like cells after brain injury.
    Article Snippet: In brief The fate of disease-associated microglia (DAM) in neuropathologies remains elusive.. Lan et al. generated an Spp1 fatemapping model to track DAM-like microglia and find that the plasticity of DAM is age dependent.. Juvenile stroke leads to the elimination of DAM, whereas neonatal DAM exhibits high plasticity and reversibility after injury, along with heightened sensitivity to subsequent immune challenges.

    Quantitative RT-PCR:

    Article Title: Intestinal-region-specific functions of AHR in intrinsic enteric neurons during infections.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit anti-HuC/D (EPR19098), Alexa Fluor 488 Abcam Cat# ab237234 Rabbit anti-HuC/D (EPR19098), Alexa Fluor 647 Abcam Cat# ab237235 AffiniPureTM Donkey Anti-Mouse IgG (H + L), CyTM5 Jackson ImmunoResearch Cat# 715-175-151; RRID: AB_2340820 AffiniPureTM Donkey Anti-Rabbit IgG (H + L), CyTM5 Jackson ImmunoResearch Cat# 711-175-152; RRID: AB_2340607 AffiniPureTM Donkey Anti-Rabbit IgG (H + L), CyTM3 Jackson ImmunoResearch Cat# 711-165-152; RRID: AB_2307443 mouse anti-HuC/D (16A11) Thermo Fisher Scientific Cat# A-21271; RRID: AB_221448 TotalSeqTM-B0451 anti-Nuclear Pore Complex Proteins Hashtag 1 Antibody Biolegend Cat# 682235; RRID:AB_2892484 TotalSeqTM-B0452 anti-Nuclear Pore Complex Proteins Hashtag 1 Antibody Biolegend Cat# 682237; RRID:AB_2892485 TotalSeqTM-B0457 anti-Nuclear Pore Complex Proteins Hashtag 1 Antibody Biolegend Cat# 682241; RRID:AB_2892487 TotalSeqTM-B0460 anti-Nuclear Pore Complex Proteins Hashtag 10 Antibody Biolegend Cat# 682249; RRID:AB_2936637 TotalSeqTM-B0461 anti-Nuclear Pore Complex Proteins Hashtag 11 Antibody Biolegend Cat# 682251; RRID:AB_2936639 TotalSeqTM-B0462 anti-Nuclear Pore Complex Proteins Hashtag 12 Antibody Biolegend Cat# 682253; RRID:AB_2936636 TotalSeqTM-B0463 anti-Nuclear Pore Complex Proteins Hashtag 13 Antibody Biolegend Cat# 682255; RRID:AB_2936630 TotalSeqTM-B0464 anti-Nuclear Pore Complex Proteins Hashtag 14 Antibody Biolegend Cat# 682245; RRID:AB_2936618 TotalSeqTM-B0301 anti-mouse Hashtag 1 Antibody Biolegend Cat# 155831; RRID:AB_2814067 TotalSeqTM-B0302 anti-mouse Hashtag 2 Antibody Biolegend Cat# 155833; RRID:AB_2814068 TotalSeqTM-B0303 anti-mouse Hashtag 3 Antibody Biolegend Cat# 155835; RRID:AB_2814069 TotalSeqTM-B0304 anti-mouse Hashtag 4 Antibody Biolegend Cat# 155837; RRID:AB_2814070 TotalSeqTM-B0305 anti-mouse Hashtag 5 Antibody Biolegend Cat# 155839; RRID:AB_2814071 TotalSeqTM-B0306 anti-mouse Hashtag 6 Antibody Biolegend Cat# 155841; RRID:AB_2814072 TotalSeqTM-B0307 anti-mouse Hashtag 7 Antibody Biolegend Cat# 155843; RRID:AB_2814073 TotalSeqTM-B0308 anti-mouse Hashtag 8 Antibody Biolegend Cat# 155845; RRID:AB_2814074 Bacterial and virus strains C. rodentium strain (DBS100) Dr. Xiaohuan Guo, Tsinghua Univeristy N/A C. albicans strain (SC5314) Dr. Guanghua Huang, Fudan Univeristy N/A Chemicals, peptides, and recombinant proteins DAPI Sigma-Aldrich Cat# D9542 Fluoromount-G SouthernBiotech Cat#0100-01 OCT SAKURA Cat# H22135 Fixable Viability Dye eFluor 780 Thermo Fisher Scientific Cat# 65-0865-14 (Continued on next page) Cell Reports 44, 115524, April 22, 2025 11 .. REAGENT or RESOURCE SOURCE IDENTIFIER Collagenase D Roche Cat# 11088882001 DNase I Roche Cat# 10104159001 RPMI-1640 Thermo Fisher Scientific Cat# 72400-047 Percoll Plus GE(cytiva) Cat# 17089109-1 Fixation/Permeabilization Solution Kit BD Biosciences Cat#554714 FBS CellMax Cat# SA211.02 DTT,DL-DITHIOTHREITOL Sigma Cat# D9779 Triton X-100 Sigma-Aldrich Cat# T8787 Normal Donkey Serum Jackson ImmunoResearch Cat# 017-000-001; RRID: AB_2337254 UltraPure DNase/RNase-Free Distilled Water Thermo Cat# 10977015 Trypan Blue solution Sigma Cat# T8154 Tween 20 Sigma-Aldrich Cat#P9416 UltraPureTM 1 M Tris-HCI Thermo Fisher Scientific Cat#15567027 5M NaCl RNase Free Thermo Fisher Scientific Cat#AM9760G MgCl2 Sigma-Aldrich Cat#M1028 CaCl2 Sigma-Aldrich Cat#21115 Bovine Serum Albumin Sigma-Aldrich Cat#A1933 Triton X-100 Diamond Cat#A110694-0100 TCL buffer QIAGEN Cat# 1031576 2-Mercaptoethanol Sigma-Aldrich Cat# M3148-25ML iScript Reverse Transcription Supermix for RT-qPCR Bio-Rad Cat# 1708841 iTaqTM Universal SYBR Green Supermix Bio-Rad Cat# 1725121 DSS MPbio Cat# 216011080 Carmine red dye Aladdin Cat# C305814 Methylcellulose Sigma-Aldrich Cat# M0512 Evans blue dye Sigma-Aldrich Cat# E2129 AIN-76A or AIN-76A supplemented with I3C (200 mg/kg) SYSEBIO Cat# PD23040401 Streptomycin sulfate Sangon Biotech A100382 Fluconazole Sigma-Aldrich PHR1160 Gentamycin sulfate Sangon Biotech A506614 Vancomycin HCl Harvey V31515 Ampicillin sodium Sangon Biotech A610028 Critical commercial assays 30 Feature Barcode Kit 10X Genomics Cat#PN-1000262 Chromium Next GEM Single Cell 30 Kit v3.1 10X Genomics Cat#PN-1000268 Dual index Kit TT Set A 10X Genomics Cat#PN-1000215 Dual index Kit NT Set A 10X Genomics Cat#PN-1000242 Deposited Data snRNA-seq and scRNA-seq This paper GEO: GSE290795 Experimental models: Organisms/strains Mouse: C57BL/6J Jackson Laboratory JAX:000664; RRID:IMSR_JAX:000664 Mouse: Tg(Actl6b-Cre)4092Jiwu/J Jackson Laboratory JAX:027826; RRID:IMSR_JAX:027826 Mouse: B6; 129-Gt(ROSA) 26Sortm5(CAG Sun1/sfGFP)Nat/J Jackson Laboratory JAX:021039; RRID:IMSR_JAX:021039 (Continued on next page) 12 Cell Reports 44, 115524, April 22, 2025 .. C56BL/6J (Jax 000664), Actl6b-Cre (Tg(Actl6b-Cre)4092Jiwu/J, Jax 027826), Ahrflox/flox (Ahrtm3.1Bra/J, Jax 006203), Sun1-GFP (B6; 129-Gt(ROSA)26Sortm5(CAG-Sun1/sfGFP)Nat/J, Jax 21039) and Syn-Cre (B6.Cg-Tg(Syn1-cre)671Jxm/J, Jax 003966) were originally obtained from the Jackson Laboratory.

    SYBR Green Assay:

    Article Title: Intestinal-region-specific functions of AHR in intrinsic enteric neurons during infections.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit anti-HuC/D (EPR19098), Alexa Fluor 488 Abcam Cat# ab237234 Rabbit anti-HuC/D (EPR19098), Alexa Fluor 647 Abcam Cat# ab237235 AffiniPureTM Donkey Anti-Mouse IgG (H + L), CyTM5 Jackson ImmunoResearch Cat# 715-175-151; RRID: AB_2340820 AffiniPureTM Donkey Anti-Rabbit IgG (H + L), CyTM5 Jackson ImmunoResearch Cat# 711-175-152; RRID: AB_2340607 AffiniPureTM Donkey Anti-Rabbit IgG (H + L), CyTM3 Jackson ImmunoResearch Cat# 711-165-152; RRID: AB_2307443 mouse anti-HuC/D (16A11) Thermo Fisher Scientific Cat# A-21271; RRID: AB_221448 TotalSeqTM-B0451 anti-Nuclear Pore Complex Proteins Hashtag 1 Antibody Biolegend Cat# 682235; RRID:AB_2892484 TotalSeqTM-B0452 anti-Nuclear Pore Complex Proteins Hashtag 1 Antibody Biolegend Cat# 682237; RRID:AB_2892485 TotalSeqTM-B0457 anti-Nuclear Pore Complex Proteins Hashtag 1 Antibody Biolegend Cat# 682241; RRID:AB_2892487 TotalSeqTM-B0460 anti-Nuclear Pore Complex Proteins Hashtag 10 Antibody Biolegend Cat# 682249; RRID:AB_2936637 TotalSeqTM-B0461 anti-Nuclear Pore Complex Proteins Hashtag 11 Antibody Biolegend Cat# 682251; RRID:AB_2936639 TotalSeqTM-B0462 anti-Nuclear Pore Complex Proteins Hashtag 12 Antibody Biolegend Cat# 682253; RRID:AB_2936636 TotalSeqTM-B0463 anti-Nuclear Pore Complex Proteins Hashtag 13 Antibody Biolegend Cat# 682255; RRID:AB_2936630 TotalSeqTM-B0464 anti-Nuclear Pore Complex Proteins Hashtag 14 Antibody Biolegend Cat# 682245; RRID:AB_2936618 TotalSeqTM-B0301 anti-mouse Hashtag 1 Antibody Biolegend Cat# 155831; RRID:AB_2814067 TotalSeqTM-B0302 anti-mouse Hashtag 2 Antibody Biolegend Cat# 155833; RRID:AB_2814068 TotalSeqTM-B0303 anti-mouse Hashtag 3 Antibody Biolegend Cat# 155835; RRID:AB_2814069 TotalSeqTM-B0304 anti-mouse Hashtag 4 Antibody Biolegend Cat# 155837; RRID:AB_2814070 TotalSeqTM-B0305 anti-mouse Hashtag 5 Antibody Biolegend Cat# 155839; RRID:AB_2814071 TotalSeqTM-B0306 anti-mouse Hashtag 6 Antibody Biolegend Cat# 155841; RRID:AB_2814072 TotalSeqTM-B0307 anti-mouse Hashtag 7 Antibody Biolegend Cat# 155843; RRID:AB_2814073 TotalSeqTM-B0308 anti-mouse Hashtag 8 Antibody Biolegend Cat# 155845; RRID:AB_2814074 Bacterial and virus strains C. rodentium strain (DBS100) Dr. Xiaohuan Guo, Tsinghua Univeristy N/A C. albicans strain (SC5314) Dr. Guanghua Huang, Fudan Univeristy N/A Chemicals, peptides, and recombinant proteins DAPI Sigma-Aldrich Cat# D9542 Fluoromount-G SouthernBiotech Cat#0100-01 OCT SAKURA Cat# H22135 Fixable Viability Dye eFluor 780 Thermo Fisher Scientific Cat# 65-0865-14 (Continued on next page) Cell Reports 44, 115524, April 22, 2025 11 .. REAGENT or RESOURCE SOURCE IDENTIFIER Collagenase D Roche Cat# 11088882001 DNase I Roche Cat# 10104159001 RPMI-1640 Thermo Fisher Scientific Cat# 72400-047 Percoll Plus GE(cytiva) Cat# 17089109-1 Fixation/Permeabilization Solution Kit BD Biosciences Cat#554714 FBS CellMax Cat# SA211.02 DTT,DL-DITHIOTHREITOL Sigma Cat# D9779 Triton X-100 Sigma-Aldrich Cat# T8787 Normal Donkey Serum Jackson ImmunoResearch Cat# 017-000-001; RRID: AB_2337254 UltraPure DNase/RNase-Free Distilled Water Thermo Cat# 10977015 Trypan Blue solution Sigma Cat# T8154 Tween 20 Sigma-Aldrich Cat#P9416 UltraPureTM 1 M Tris-HCI Thermo Fisher Scientific Cat#15567027 5M NaCl RNase Free Thermo Fisher Scientific Cat#AM9760G MgCl2 Sigma-Aldrich Cat#M1028 CaCl2 Sigma-Aldrich Cat#21115 Bovine Serum Albumin Sigma-Aldrich Cat#A1933 Triton X-100 Diamond Cat#A110694-0100 TCL buffer QIAGEN Cat# 1031576 2-Mercaptoethanol Sigma-Aldrich Cat# M3148-25ML iScript Reverse Transcription Supermix for RT-qPCR Bio-Rad Cat# 1708841 iTaqTM Universal SYBR Green Supermix Bio-Rad Cat# 1725121 DSS MPbio Cat# 216011080 Carmine red dye Aladdin Cat# C305814 Methylcellulose Sigma-Aldrich Cat# M0512 Evans blue dye Sigma-Aldrich Cat# E2129 AIN-76A or AIN-76A supplemented with I3C (200 mg/kg) SYSEBIO Cat# PD23040401 Streptomycin sulfate Sangon Biotech A100382 Fluconazole Sigma-Aldrich PHR1160 Gentamycin sulfate Sangon Biotech A506614 Vancomycin HCl Harvey V31515 Ampicillin sodium Sangon Biotech A610028 Critical commercial assays 30 Feature Barcode Kit 10X Genomics Cat#PN-1000262 Chromium Next GEM Single Cell 30 Kit v3.1 10X Genomics Cat#PN-1000268 Dual index Kit TT Set A 10X Genomics Cat#PN-1000215 Dual index Kit NT Set A 10X Genomics Cat#PN-1000242 Deposited Data snRNA-seq and scRNA-seq This paper GEO: GSE290795 Experimental models: Organisms/strains Mouse: C57BL/6J Jackson Laboratory JAX:000664; RRID:IMSR_JAX:000664 Mouse: Tg(Actl6b-Cre)4092Jiwu/J Jackson Laboratory JAX:027826; RRID:IMSR_JAX:027826 Mouse: B6; 129-Gt(ROSA) 26Sortm5(CAG Sun1/sfGFP)Nat/J Jackson Laboratory JAX:021039; RRID:IMSR_JAX:021039 (Continued on next page) 12 Cell Reports 44, 115524, April 22, 2025 .. C56BL/6J (Jax 000664), Actl6b-Cre (Tg(Actl6b-Cre)4092Jiwu/J, Jax 027826), Ahrflox/flox (Ahrtm3.1Bra/J, Jax 006203), Sun1-GFP (B6; 129-Gt(ROSA)26Sortm5(CAG-Sun1/sfGFP)Nat/J, Jax 21039) and Syn-Cre (B6.Cg-Tg(Syn1-cre)671Jxm/J, Jax 003966) were originally obtained from the Jackson Laboratory.

    Article Title: Fate mapping of Spp1 expression reveals age-dependent plasticity of disease-associated microglia-like cells after brain injury.
    Article Snippet: In brief The fate of disease-associated microglia (DAM) in neuropathologies remains elusive.. Lan et al. generated an Spp1 fatemapping model to track DAM-like microglia and find that the plasticity of DAM is age dependent.. Juvenile stroke leads to the elimination of DAM, whereas neonatal DAM exhibits high plasticity and reversibility after injury, along with heightened sensitivity to subsequent immune challenges.

    Single Cell:

    Article Title: Intestinal-region-specific functions of AHR in intrinsic enteric neurons during infections.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit anti-HuC/D (EPR19098), Alexa Fluor 488 Abcam Cat# ab237234 Rabbit anti-HuC/D (EPR19098), Alexa Fluor 647 Abcam Cat# ab237235 AffiniPureTM Donkey Anti-Mouse IgG (H + L), CyTM5 Jackson ImmunoResearch Cat# 715-175-151; RRID: AB_2340820 AffiniPureTM Donkey Anti-Rabbit IgG (H + L), CyTM5 Jackson ImmunoResearch Cat# 711-175-152; RRID: AB_2340607 AffiniPureTM Donkey Anti-Rabbit IgG (H + L), CyTM3 Jackson ImmunoResearch Cat# 711-165-152; RRID: AB_2307443 mouse anti-HuC/D (16A11) Thermo Fisher Scientific Cat# A-21271; RRID: AB_221448 TotalSeqTM-B0451 anti-Nuclear Pore Complex Proteins Hashtag 1 Antibody Biolegend Cat# 682235; RRID:AB_2892484 TotalSeqTM-B0452 anti-Nuclear Pore Complex Proteins Hashtag 1 Antibody Biolegend Cat# 682237; RRID:AB_2892485 TotalSeqTM-B0457 anti-Nuclear Pore Complex Proteins Hashtag 1 Antibody Biolegend Cat# 682241; RRID:AB_2892487 TotalSeqTM-B0460 anti-Nuclear Pore Complex Proteins Hashtag 10 Antibody Biolegend Cat# 682249; RRID:AB_2936637 TotalSeqTM-B0461 anti-Nuclear Pore Complex Proteins Hashtag 11 Antibody Biolegend Cat# 682251; RRID:AB_2936639 TotalSeqTM-B0462 anti-Nuclear Pore Complex Proteins Hashtag 12 Antibody Biolegend Cat# 682253; RRID:AB_2936636 TotalSeqTM-B0463 anti-Nuclear Pore Complex Proteins Hashtag 13 Antibody Biolegend Cat# 682255; RRID:AB_2936630 TotalSeqTM-B0464 anti-Nuclear Pore Complex Proteins Hashtag 14 Antibody Biolegend Cat# 682245; RRID:AB_2936618 TotalSeqTM-B0301 anti-mouse Hashtag 1 Antibody Biolegend Cat# 155831; RRID:AB_2814067 TotalSeqTM-B0302 anti-mouse Hashtag 2 Antibody Biolegend Cat# 155833; RRID:AB_2814068 TotalSeqTM-B0303 anti-mouse Hashtag 3 Antibody Biolegend Cat# 155835; RRID:AB_2814069 TotalSeqTM-B0304 anti-mouse Hashtag 4 Antibody Biolegend Cat# 155837; RRID:AB_2814070 TotalSeqTM-B0305 anti-mouse Hashtag 5 Antibody Biolegend Cat# 155839; RRID:AB_2814071 TotalSeqTM-B0306 anti-mouse Hashtag 6 Antibody Biolegend Cat# 155841; RRID:AB_2814072 TotalSeqTM-B0307 anti-mouse Hashtag 7 Antibody Biolegend Cat# 155843; RRID:AB_2814073 TotalSeqTM-B0308 anti-mouse Hashtag 8 Antibody Biolegend Cat# 155845; RRID:AB_2814074 Bacterial and virus strains C. rodentium strain (DBS100) Dr. Xiaohuan Guo, Tsinghua Univeristy N/A C. albicans strain (SC5314) Dr. Guanghua Huang, Fudan Univeristy N/A Chemicals, peptides, and recombinant proteins DAPI Sigma-Aldrich Cat# D9542 Fluoromount-G SouthernBiotech Cat#0100-01 OCT SAKURA Cat# H22135 Fixable Viability Dye eFluor 780 Thermo Fisher Scientific Cat# 65-0865-14 (Continued on next page) Cell Reports 44, 115524, April 22, 2025 11 .. REAGENT or RESOURCE SOURCE IDENTIFIER Collagenase D Roche Cat# 11088882001 DNase I Roche Cat# 10104159001 RPMI-1640 Thermo Fisher Scientific Cat# 72400-047 Percoll Plus GE(cytiva) Cat# 17089109-1 Fixation/Permeabilization Solution Kit BD Biosciences Cat#554714 FBS CellMax Cat# SA211.02 DTT,DL-DITHIOTHREITOL Sigma Cat# D9779 Triton X-100 Sigma-Aldrich Cat# T8787 Normal Donkey Serum Jackson ImmunoResearch Cat# 017-000-001; RRID: AB_2337254 UltraPure DNase/RNase-Free Distilled Water Thermo Cat# 10977015 Trypan Blue solution Sigma Cat# T8154 Tween 20 Sigma-Aldrich Cat#P9416 UltraPureTM 1 M Tris-HCI Thermo Fisher Scientific Cat#15567027 5M NaCl RNase Free Thermo Fisher Scientific Cat#AM9760G MgCl2 Sigma-Aldrich Cat#M1028 CaCl2 Sigma-Aldrich Cat#21115 Bovine Serum Albumin Sigma-Aldrich Cat#A1933 Triton X-100 Diamond Cat#A110694-0100 TCL buffer QIAGEN Cat# 1031576 2-Mercaptoethanol Sigma-Aldrich Cat# M3148-25ML iScript Reverse Transcription Supermix for RT-qPCR Bio-Rad Cat# 1708841 iTaqTM Universal SYBR Green Supermix Bio-Rad Cat# 1725121 DSS MPbio Cat# 216011080 Carmine red dye Aladdin Cat# C305814 Methylcellulose Sigma-Aldrich Cat# M0512 Evans blue dye Sigma-Aldrich Cat# E2129 AIN-76A or AIN-76A supplemented with I3C (200 mg/kg) SYSEBIO Cat# PD23040401 Streptomycin sulfate Sangon Biotech A100382 Fluconazole Sigma-Aldrich PHR1160 Gentamycin sulfate Sangon Biotech A506614 Vancomycin HCl Harvey V31515 Ampicillin sodium Sangon Biotech A610028 Critical commercial assays 30 Feature Barcode Kit 10X Genomics Cat#PN-1000262 Chromium Next GEM Single Cell 30 Kit v3.1 10X Genomics Cat#PN-1000268 Dual index Kit TT Set A 10X Genomics Cat#PN-1000215 Dual index Kit NT Set A 10X Genomics Cat#PN-1000242 Deposited Data snRNA-seq and scRNA-seq This paper GEO: GSE290795 Experimental models: Organisms/strains Mouse: C57BL/6J Jackson Laboratory JAX:000664; RRID:IMSR_JAX:000664 Mouse: Tg(Actl6b-Cre)4092Jiwu/J Jackson Laboratory JAX:027826; RRID:IMSR_JAX:027826 Mouse: B6; 129-Gt(ROSA) 26Sortm5(CAG Sun1/sfGFP)Nat/J Jackson Laboratory JAX:021039; RRID:IMSR_JAX:021039 (Continued on next page) 12 Cell Reports 44, 115524, April 22, 2025 .. C56BL/6J (Jax 000664), Actl6b-Cre (Tg(Actl6b-Cre)4092Jiwu/J, Jax 027826), Ahrflox/flox (Ahrtm3.1Bra/J, Jax 006203), Sun1-GFP (B6; 129-Gt(ROSA)26Sortm5(CAG-Sun1/sfGFP)Nat/J, Jax 21039) and Syn-Cre (B6.Cg-Tg(Syn1-cre)671Jxm/J, Jax 003966) were originally obtained from the Jackson Laboratory.

    Article Title: Fate mapping of Spp1 expression reveals age-dependent plasticity of disease-associated microglia-like cells after brain injury.
    Article Snippet: In brief The fate of disease-associated microglia (DAM) in neuropathologies remains elusive.. Lan et al. generated an Spp1 fatemapping model to track DAM-like microglia and find that the plasticity of DAM is age dependent.. Juvenile stroke leads to the elimination of DAM, whereas neonatal DAM exhibits high plasticity and reversibility after injury, along with heightened sensitivity to subsequent immune challenges.

    cDNA Synthesis:

    Article Title: Fate mapping of Spp1 expression reveals age-dependent plasticity of disease-associated microglia-like cells after brain injury.
    Article Snippet: In brief The fate of disease-associated microglia (DAM) in neuropathologies remains elusive.. Lan et al. generated an Spp1 fatemapping model to track DAM-like microglia and find that the plasticity of DAM is age dependent.. Juvenile stroke leads to the elimination of DAM, whereas neonatal DAM exhibits high plasticity and reversibility after injury, along with heightened sensitivity to subsequent immune challenges.

    TUNEL Assay:

    Article Title: Fate mapping of Spp1 expression reveals age-dependent plasticity of disease-associated microglia-like cells after brain injury.
    Article Snippet: In brief The fate of disease-associated microglia (DAM) in neuropathologies remains elusive.. Lan et al. generated an Spp1 fatemapping model to track DAM-like microglia and find that the plasticity of DAM is age dependent.. Juvenile stroke leads to the elimination of DAM, whereas neonatal DAM exhibits high plasticity and reversibility after injury, along with heightened sensitivity to subsequent immune challenges.

    Polymerase Chain Reaction:

    Article Title: Fate mapping of Spp1 expression reveals age-dependent plasticity of disease-associated microglia-like cells after brain injury.
    Article Snippet: In brief The fate of disease-associated microglia (DAM) in neuropathologies remains elusive.. Lan et al. generated an Spp1 fatemapping model to track DAM-like microglia and find that the plasticity of DAM is age dependent.. Juvenile stroke leads to the elimination of DAM, whereas neonatal DAM exhibits high plasticity and reversibility after injury, along with heightened sensitivity to subsequent immune challenges.

    RNA sequencing:

    Article Title: Fate mapping of Spp1 expression reveals age-dependent plasticity of disease-associated microglia-like cells after brain injury.
    Article Snippet: In brief The fate of disease-associated microglia (DAM) in neuropathologies remains elusive.. Lan et al. generated an Spp1 fatemapping model to track DAM-like microglia and find that the plasticity of DAM is age dependent.. Juvenile stroke leads to the elimination of DAM, whereas neonatal DAM exhibits high plasticity and reversibility after injury, along with heightened sensitivity to subsequent immune challenges.



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