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Larex Inc percoll-larex gradients
Percoll Larex Gradients, supplied by Larex Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/percoll+gradient/percoll+larex+gradients/us10632212-463-5-7
Average 90 stars, based on 1 article reviews
percoll-larex gradients - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Centrifugation:

Article Title: Oxidative status of valinomycin-resistant normal, beta-thalassemia and sickle red blood cells.
Article Snippet: Exposure of red blood cells (RBC) to the K-ionophore valinomycin (val), causes loss of KCl and water, resulting in cell dehydration, manifested by increased cell density.. While almost all normal val-treated RBC dehydrate, in sickle cell anemia (SCA) a portion of the RBC fail to dehydrate and maintain a light density, indicating the existence of val-resistant (val-res) RBC.. In thalassemia and sickle cell disease (SCD), although the primary lesion is in the globin genes, damage to the RBC is partly mediated by oxidative stress.

other:

Article Title: Hemoglobin C in transgenic mice: effect of HbC expression from founders to full mouse globin knockouts.
Article Snippet: When present in the homozygous form, hemoglobin C (HbC, CC disease) increases red cell density, a feature that is the major factor underlying the pathology in patients with SC disease (Fabry et al., JCI 70, 1315, 1982).. The basis for the increased red cell density has not yet been fully defined.. We have generated a HbC mouse in which the most successful founder expresses 56% human a and 34% human b.

Article Title: Therapeutic retroviral vectors for gene therapy
Article Snippet: RBC densities were examined on Percoll-Larex gradients (Larex International, St Paul), as described in Fabry et al. (1984) Blood 90:3332.

Article Title: Second generation knockout sickle mice: the effect of HbF.
Article Snippet: Sickle transgenic mice expressing exclusively human globins are desirable for studying pathophysiology and testing gene therapy strategies, but they must have significant pathology and show evidence of amelioration by antisickling hemoglobins.. Mice were generated that expressed exclusively human sickle hemoglobin with 3 levels of HbF using their previously described sickle constructs (cointegrated human miniLCRa2 and miniLCRbS [PNAS 89:12150, 1992]), mouse aand b-globin-knockouts, and 3 different human g-transgenes.. It was found that, at all 3 levels of HbF expression, these mice have balanced chain synthesis, nearly normal mean corpuscular hemoglobin, and, in some cases, F cells.

Article Title: Phase Separation and Crystallization of Hemoglobin C in Transgenic Mouse and Human Erythrocytes
Article Snippet: Liquid-liquid phase separation To test whether L-L phase separation occurs in the cytosol of RBC, two sources of HbC containing RBC were studied: 1), the RBC dense fraction (separated on a Percoll-Larex gradient) obtained from human CC patients; and 2), RBC from transgenic mice expressing exclusively human HbC ( 31 ).

Article Title: Therapeutic retroviral vectors for gene therapy
Article Snippet: RBC densities were examined on Percoll-Larex gradients (Larex International, St Paul), as described in Fabry et al. (1984) Blood 90:3332.

Article Title: Arginine supplementation of sickle transgenic mice reduces red cell density and Gardos channel activity.
Article Snippet: Nitric oxide (NO), essential for maintaining vascular tone, is produced from arginine by nitric oxide synthase.. Plasma arginine levels are low in sickle cell anemia, and it is reported here that low plasma arginine is also found in our sickle transgenic mouse model that expresses human a, human bS, and human bS-Antilles and is homozygous for the mouse bmajor deletion (S1S-Antilles).. S1S-Antilles mice were supplemented with a 4-fold increase in arginine that was maintained for several months.

Article Title: Therapeutic retroviral vectors for gene therapy
Article Snippet: RBC densities were examined on Percoll-Larex gradients (Larex International, St Paul), as described in Fabry et al. (1984) Blood 90:3332.



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