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Pharmacia LKB Biotechnology Inc discontinuous percoll gradient
Discontinuous Percoll Gradient, supplied by Pharmacia LKB Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/percoll+gradient/discontinuous+percoll+gradient/pm09512252-52-5-8
Average 90 stars, based on 1 article reviews
discontinuous percoll gradient - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Centrifugation:

Article Title: Expression of interleukin-1 system genes in human gametes.
Article Snippet: .. Semen was liquefied at room temperature for 30 min. After centrifugation at 500 3 g for 30 min at room temperature, semen cells were fractionated on discontinuous Percoll gradients (Pharmacia LKB Nuclear Inc., Gaithersburg, MD) consisting of 90%, 70%, and 45% layers. .. Cells harvested from interfaces— which we designated a (0–45%), b (45–70%), c (70–90%), and d (pellet) d—were washed once with Ham’s F-10 with HEPES (Gibco BRL Life Technologies, Grand Island, NY).

Article Title: Shedding of tumour necrosis factor receptors from purified villous term trophoblasts and cytotrophoblastic BeWo cells.
Article Snippet: .. Cells were then pooled by centrifugation, resuspended in DMEM and layered on top of a discontinuous Percoll gradient (Pharmacia LKB Biotechnology, Uppsala, Sweden) ranging from 63% to 4.5%. ..

Staining:

Article Title: Role of alveolar macrophages in innate immunity in neonates: evidence for selective lipopolysaccharide binding protein production by rat neonatal alveolar macrophages.
Article Snippet: As the first line of defense against inhaled substances, alveolar macrophages (AM) play a crucial role in maintaining lung homeostasis.. This is achieved via phagocytosis of foreign material and the secretion of a wide range of mediator molecules, including those involved in neutrophil recruitment.. Neonates are known to manifest increased susceptibility to lung infections, and we hypothesize that this may be due in part to a deficiency in the function of AM.

Purification:

Article Title: Role of alveolar macrophages in innate immunity in neonates: evidence for selective lipopolysaccharide binding protein production by rat neonatal alveolar macrophages.
Article Snippet: As the first line of defense against inhaled substances, alveolar macrophages (AM) play a crucial role in maintaining lung homeostasis.. This is achieved via phagocytosis of foreign material and the secretion of a wide range of mediator molecules, including those involved in neutrophil recruitment.. Neonates are known to manifest increased susceptibility to lung infections, and we hypothesize that this may be due in part to a deficiency in the function of AM.

other:

Article Title: Estradiol-17 beta and progesterone increase ovine uterine suppressor cell activity.
Article Snippet: We evaluated the regulation of ovine uterine (UT) suppressor cell activity by progesterone (P4) , estradiol-17b (E2) , and P4 + E2 in ovariectomized (OVX) ewes.. Following 14 d of steroid injections, endometrial cells (designated as UT cells) were recovered postmortem, and unfractionated and fractionated cells were assessed for suppression of autologous phytohemagglutinin (PHA)-treated peripheral blood lymphocytes (PBL).. Supernatants from cultured UT cells were also assessed for suppressor activity.



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