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mutagenesis human mcoln3 trpml3 in pegfpc1  (TaKaRa)


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    Structured Review

    TaKaRa mutagenesis human mcoln3 trpml3 in pegfpc1
    Mutagenesis Human Mcoln3 Trpml3 In Pegfpc1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 776 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfpc1+vector/pCMV-HA+Vector+Set/pmc06333453-409-3-8
    Average 96 stars, based on 776 article reviews
    mutagenesis human mcoln3 trpml3 in pegfpc1 - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Epigenetic Silencing of CYYR1 Promotes Lung Adenocarcinoma Progression Through TRIM21-Dependent Regulation of HMMR Stability.
    Article Snippet: .. pCMV-HA vector (Clontech) was used to express ubiquitin Ub). ..

    Article Title: An alternative reverse genetics system for PRRS virus and its application to define the role of endocytic sorting signal in GP3 protein intracellular trafficking
    Article Snippet: .. It was based on the pCMV vector (Clontech, Takara Bio, Mountain View, CA, United States) in which the internal intron and multiple cloning sites between the TATA box and the viral genome were deleted to minimize non-viral leader sequences. ..

    Article Title: Identification of host proteins interacting with NS3 protein of Bluetongue virus
    Article Snippet: .. The DNA fragment NS3t was amplified and sub-cloned in pCMV-HA-N vector (Clontech, USA). ..

    Article Title: Seneca Valley virus 2B and 3 C proteins attenuate the cGAS-STING signaling pathway by targeting STING for degradation.
    Article Snippet: Seneca Valley virus (SVV), an emerging porcine picornavirus, has attracted significant attention recently as it presents a substantial threat to the global pork industry.. Stimulator of interferon genes (STING) is an endoplasmic reticulum (ER)-associated adaptor protein that plays a crucial role in the type I interferon (IFN-I) signaling pathway, which triggers IFN-stimulated gene (ISG) production to elicit antiviral innate immunity.. SVV has evolved numerous strategies to counteract the IFN-I signaling pathway and evade innate immunity.

    Article Title: A Fish MDA5 Integrates Canonical RIG-I and MDA5 Ligand Recognition.
    Article Snippet: .. The Lc MAVS (XM_010756308.3) expression plasmid was generated by inserting its ORF into the pCMVHA-C (Clontech) vector. .. Plasmids for Flag- and Myc-tagged Lc STING (XM_010732873.3) expression were constructed by cloning the ORF into the p3xFLAG-CMV-14 and pcDNA3.1/mycHis( + ) A (Sigma-Aldrich) vectors, respectively.

    Ubiquitin Proteomics:

    Article Title: Epigenetic Silencing of CYYR1 Promotes Lung Adenocarcinoma Progression Through TRIM21-Dependent Regulation of HMMR Stability.
    Article Snippet: .. pCMV-HA vector (Clontech) was used to express ubiquitin Ub). ..

    Hemagglutination Assay:

    Article Title: Acetylation promotes thyroid hormone receptor ubiquitination and degradation by nuclear proteasomes.
    Article Snippet: cDNAs for TRβ1 single site acetylation mimics (K184Q, K188Q, or K190Q) and TRβ1 single site nonacetylation mimics (K184R, K188R, or K190R) were synthesized by Invitrogen GeneArt Gene Synthesis (Thermo Fisher Scientific) and subcloned into the pEGFP-C1 vector (Clontech). .. HA-TRα1, HA-TRβ1, HA-TRβ2, and triple site acetylation and nonacetylation mimics were each constructed using PCR-modified GFP-TRα1, GFP-TRβ1, and GFP-TRβ2 insertions into pCMV-HA-N vectors (Clontech). .. Coding regions for C392X TRα1 acetylation and nonacetylation mimics were acquired from Invitrogen GeneArt Gene Synthesis (Thermo Fisher Scientific) and subcloned into pCMV-HA-N and pEGFP-C1.

    Construct:

    Article Title: Acetylation promotes thyroid hormone receptor ubiquitination and degradation by nuclear proteasomes.
    Article Snippet: cDNAs for TRβ1 single site acetylation mimics (K184Q, K188Q, or K190Q) and TRβ1 single site nonacetylation mimics (K184R, K188R, or K190R) were synthesized by Invitrogen GeneArt Gene Synthesis (Thermo Fisher Scientific) and subcloned into the pEGFP-C1 vector (Clontech). .. HA-TRα1, HA-TRβ1, HA-TRβ2, and triple site acetylation and nonacetylation mimics were each constructed using PCR-modified GFP-TRα1, GFP-TRβ1, and GFP-TRβ2 insertions into pCMV-HA-N vectors (Clontech). .. Coding regions for C392X TRα1 acetylation and nonacetylation mimics were acquired from Invitrogen GeneArt Gene Synthesis (Thermo Fisher Scientific) and subcloned into pCMV-HA-N and pEGFP-C1.

    Article Title: Seneca Valley virus 2B and 3 C proteins attenuate the cGAS-STING signaling pathway by targeting STING for degradation.
    Article Snippet: Seneca Valley virus (SVV), an emerging porcine picornavirus, has attracted significant attention recently as it presents a substantial threat to the global pork industry.. Stimulator of interferon genes (STING) is an endoplasmic reticulum (ER)-associated adaptor protein that plays a crucial role in the type I interferon (IFN-I) signaling pathway, which triggers IFN-stimulated gene (ISG) production to elicit antiviral innate immunity.. SVV has evolved numerous strategies to counteract the IFN-I signaling pathway and evade innate immunity.

    Polymerase Chain Reaction:

    Article Title: Acetylation promotes thyroid hormone receptor ubiquitination and degradation by nuclear proteasomes.
    Article Snippet: cDNAs for TRβ1 single site acetylation mimics (K184Q, K188Q, or K190Q) and TRβ1 single site nonacetylation mimics (K184R, K188R, or K190R) were synthesized by Invitrogen GeneArt Gene Synthesis (Thermo Fisher Scientific) and subcloned into the pEGFP-C1 vector (Clontech). .. HA-TRα1, HA-TRβ1, HA-TRβ2, and triple site acetylation and nonacetylation mimics were each constructed using PCR-modified GFP-TRα1, GFP-TRβ1, and GFP-TRβ2 insertions into pCMV-HA-N vectors (Clontech). .. Coding regions for C392X TRα1 acetylation and nonacetylation mimics were acquired from Invitrogen GeneArt Gene Synthesis (Thermo Fisher Scientific) and subcloned into pCMV-HA-N and pEGFP-C1.

    other:

    Article Title: Seneca Valley virus 3C protease targets the Nrf2/HO-1 pathway to antagonize its antiviral activity
    Article Snippet: TBHQ (HY-100489), ML385 (HY-100523), Cobalt protoporphyrin IX (CoPP, HY-W250116), ZnPP (HY-101193), CORM-2 (HY-W033577), BV (HY-135005A), NAC (HY-B0215), bilirubin (HY-N0323, BR), ODQ (HY-101255, an sGC inhibitor), and KT5823 (HY-N6791, a PKG inhibitor) were obtained from MedChemExpress (Shanghai, China).

    Cloning:

    Article Title: An alternative reverse genetics system for PRRS virus and its application to define the role of endocytic sorting signal in GP3 protein intracellular trafficking
    Article Snippet: .. It was based on the pCMV vector (Clontech, Takara Bio, Mountain View, CA, United States) in which the internal intron and multiple cloning sites between the TATA box and the viral genome were deleted to minimize non-viral leader sequences. ..

    Amplification:

    Article Title: Identification of host proteins interacting with NS3 protein of Bluetongue virus
    Article Snippet: .. The DNA fragment NS3t was amplified and sub-cloned in pCMV-HA-N vector (Clontech, USA). ..

    Synthesized:

    Article Title: Seneca Valley virus 2B and 3 C proteins attenuate the cGAS-STING signaling pathway by targeting STING for degradation.
    Article Snippet: Seneca Valley virus (SVV), an emerging porcine picornavirus, has attracted significant attention recently as it presents a substantial threat to the global pork industry.. Stimulator of interferon genes (STING) is an endoplasmic reticulum (ER)-associated adaptor protein that plays a crucial role in the type I interferon (IFN-I) signaling pathway, which triggers IFN-stimulated gene (ISG) production to elicit antiviral innate immunity.. SVV has evolved numerous strategies to counteract the IFN-I signaling pathway and evade innate immunity.

    Clone Assay:

    Article Title: A helix-loop-helix gene orchestrates male fate in common carp
    Article Snippet: Promoter regions of cyp19a1a-A and cyp19a1a-B were amplified and cloned into pGL3-Basic (Promega, USA; catalog no. E1751) to generate luciferase reporter constructs (table S16). .. The coding sequences of common carp nr5a1a and foxl2b were cloned into p3×FLAG-CMV-10 (Sigma-Aldrich, USA; catalog no. E7658), and the full-length pangu coding sequence and a 288-bp deletion variant (from XY pangu Δ288 individuals) were cloned into pCMV-HA (Clontech/Takara Bio USA; catalog no. 635690). ..

    Sequencing:

    Article Title: A helix-loop-helix gene orchestrates male fate in common carp
    Article Snippet: Promoter regions of cyp19a1a-A and cyp19a1a-B were amplified and cloned into pGL3-Basic (Promega, USA; catalog no. E1751) to generate luciferase reporter constructs (table S16). .. The coding sequences of common carp nr5a1a and foxl2b were cloned into p3×FLAG-CMV-10 (Sigma-Aldrich, USA; catalog no. E7658), and the full-length pangu coding sequence and a 288-bp deletion variant (from XY pangu Δ288 individuals) were cloned into pCMV-HA (Clontech/Takara Bio USA; catalog no. 635690). ..

    Variant Assay:

    Article Title: A helix-loop-helix gene orchestrates male fate in common carp
    Article Snippet: Promoter regions of cyp19a1a-A and cyp19a1a-B were amplified and cloned into pGL3-Basic (Promega, USA; catalog no. E1751) to generate luciferase reporter constructs (table S16). .. The coding sequences of common carp nr5a1a and foxl2b were cloned into p3×FLAG-CMV-10 (Sigma-Aldrich, USA; catalog no. E7658), and the full-length pangu coding sequence and a 288-bp deletion variant (from XY pangu Δ288 individuals) were cloned into pCMV-HA (Clontech/Takara Bio USA; catalog no. 635690). ..

    Expressing:

    Article Title: A Fish MDA5 Integrates Canonical RIG-I and MDA5 Ligand Recognition.
    Article Snippet: .. The Lc MAVS (XM_010756308.3) expression plasmid was generated by inserting its ORF into the pCMVHA-C (Clontech) vector. .. Plasmids for Flag- and Myc-tagged Lc STING (XM_010732873.3) expression were constructed by cloning the ORF into the p3xFLAG-CMV-14 and pcDNA3.1/mycHis( + ) A (Sigma-Aldrich) vectors, respectively.

    Generated:

    Article Title: A Fish MDA5 Integrates Canonical RIG-I and MDA5 Ligand Recognition.
    Article Snippet: .. The Lc MAVS (XM_010756308.3) expression plasmid was generated by inserting its ORF into the pCMVHA-C (Clontech) vector. .. Plasmids for Flag- and Myc-tagged Lc STING (XM_010732873.3) expression were constructed by cloning the ORF into the p3xFLAG-CMV-14 and pcDNA3.1/mycHis( + ) A (Sigma-Aldrich) vectors, respectively.



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    HEK293T cells were transfected with <t>pEGFPC1</t> vector or pEGFPC1 plasmids carrying TF or 6K. Twenty-four hours post-transfection, cells were fixed, and immunostaining was performed for Scribble (red). Inset shows zoomed images of an EGFP-TF expressing cell. Arrowheads indicate Scribble puncta and co-localization with TF (A). Mutational analysis of TF and its effect on Scribble localization (B). Expression data of TF and TF mutants in HEK293T cells. Twenty-four hours post-transfection, cells were lysed, and 6K and TF expression were analyzed using western blot with anti-GFP antibody (C). L: protein ladder, C1: pEGFPC1 empty vector, TF Mut1: TF-HGAATV, TF Mut2: TF-AAAATV.
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    HEK293T cells were transfected with <t>pEGFPC1</t> vector or pEGFPC1 plasmids carrying TF or 6K. Twenty-four hours post-transfection, cells were fixed, and immunostaining was performed for Scribble (red). Inset shows zoomed images of an EGFP-TF expressing cell. Arrowheads indicate Scribble puncta and co-localization with TF (A). Mutational analysis of TF and its effect on Scribble localization (B). Expression data of TF and TF mutants in HEK293T cells. Twenty-four hours post-transfection, cells were lysed, and 6K and TF expression were analyzed using western blot with anti-GFP antibody (C). L: protein ladder, C1: pEGFPC1 empty vector, TF Mut1: TF-HGAATV, TF Mut2: TF-AAAATV.
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    HEK293T cells were transfected with <t>pEGFPC1</t> vector or pEGFPC1 plasmids carrying TF or 6K. Twenty-four hours post-transfection, cells were fixed, and immunostaining was performed for Scribble (red). Inset shows zoomed images of an EGFP-TF expressing cell. Arrowheads indicate Scribble puncta and co-localization with TF (A). Mutational analysis of TF and its effect on Scribble localization (B). Expression data of TF and TF mutants in HEK293T cells. Twenty-four hours post-transfection, cells were lysed, and 6K and TF expression were analyzed using western blot with anti-GFP antibody (C). L: protein ladder, C1: pEGFPC1 empty vector, TF Mut1: TF-HGAATV, TF Mut2: TF-AAAATV.
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    Image Search Results


    Journal: eLife

    Article Title: SAFB regulates hippocampal stem cell fate by targeting Drosha to destabilize Nfib mRNA

    doi: 10.7554/eLife.74940

    Figure Lengend Snippet:

    Article Snippet: Recombinant DNA reagent , pEGFPC1-6XHis- FLKSRP (plasmid) , , RRID: Addgene_23001 , pEGFPC1 expression vector.

    Techniques: Protease Inhibitor, Recombinant, Clone Assay, Bicinchoninic Acid Protein Assay, Mutagenesis, Transfection, Sequencing, esiRNA, Blocking Assay, Plasmid Preparation, Expressing, Software

    HEK293T cells were transfected with pEGFPC1 vector or pEGFPC1 plasmids carrying TF or 6K. Twenty-four hours post-transfection, cells were fixed, and immunostaining was performed for Scribble (red). Inset shows zoomed images of an EGFP-TF expressing cell. Arrowheads indicate Scribble puncta and co-localization with TF (A). Mutational analysis of TF and its effect on Scribble localization (B). Expression data of TF and TF mutants in HEK293T cells. Twenty-four hours post-transfection, cells were lysed, and 6K and TF expression were analyzed using western blot with anti-GFP antibody (C). L: protein ladder, C1: pEGFPC1 empty vector, TF Mut1: TF-HGAATV, TF Mut2: TF-AAAATV.

    Journal: bioRxiv

    Article Title: Downregulation of a cell polarity protein potentiates Chikungunya Virus infection in host cells

    doi: 10.1101/2023.07.24.550336

    Figure Lengend Snippet: HEK293T cells were transfected with pEGFPC1 vector or pEGFPC1 plasmids carrying TF or 6K. Twenty-four hours post-transfection, cells were fixed, and immunostaining was performed for Scribble (red). Inset shows zoomed images of an EGFP-TF expressing cell. Arrowheads indicate Scribble puncta and co-localization with TF (A). Mutational analysis of TF and its effect on Scribble localization (B). Expression data of TF and TF mutants in HEK293T cells. Twenty-four hours post-transfection, cells were lysed, and 6K and TF expression were analyzed using western blot with anti-GFP antibody (C). L: protein ladder, C1: pEGFPC1 empty vector, TF Mut1: TF-HGAATV, TF Mut2: TF-AAAATV.

    Article Snippet: The cDNA sequences of CHIKV 6K and TF (Supplementary Figure S1) were cloned in the pEGFPC1 vector (Clontech) to generate fusion proteins tagged with the Enhanced Green Fluorescent Protein (EGFP) at the N-terminus.

    Techniques: Transfection, Plasmid Preparation, Immunostaining, Expressing, Western Blot

    HEK293T cells were infected with CHIKV at 0.1 MOI. Scribble levels were analyzed at 24 and 48 hpi using western blot and quantitated using densitometry (A). Twenty-four hours post-infection, the cells were fixed, and immunostaining was performed for Scribble and CHIKV capsid and E2 proteins. Inset shows zoomed images of an E2 expressing cell. Arrowheads indicate Scribble puncta (B). Effect of TF on Scribble expression. HEK293T cells were transfected with pEGFPC1-TF and its mutants. Only vector and pEGFPC1-6K were also transfected, and Scribble levels were analyzed 48 h post-transfection using western blot and quantitated using densitometry (C). Electron microscopy (EM) imaging of CHIKV-infected HEK293T cells. HEK293T cells were infected with CHIKV at 1.0 MOI and Scribble was visualized using a gold-labeled secondary antibody (D-i). EM images of an infected cell at higher magnification (D-ii). Immunostaining for ubiquitin and Scribble in CHIKV infected HEK293T cells (E). Data represents mean ± SEM of two biological replicates.

    Journal: bioRxiv

    Article Title: Downregulation of a cell polarity protein potentiates Chikungunya Virus infection in host cells

    doi: 10.1101/2023.07.24.550336

    Figure Lengend Snippet: HEK293T cells were infected with CHIKV at 0.1 MOI. Scribble levels were analyzed at 24 and 48 hpi using western blot and quantitated using densitometry (A). Twenty-four hours post-infection, the cells were fixed, and immunostaining was performed for Scribble and CHIKV capsid and E2 proteins. Inset shows zoomed images of an E2 expressing cell. Arrowheads indicate Scribble puncta (B). Effect of TF on Scribble expression. HEK293T cells were transfected with pEGFPC1-TF and its mutants. Only vector and pEGFPC1-6K were also transfected, and Scribble levels were analyzed 48 h post-transfection using western blot and quantitated using densitometry (C). Electron microscopy (EM) imaging of CHIKV-infected HEK293T cells. HEK293T cells were infected with CHIKV at 1.0 MOI and Scribble was visualized using a gold-labeled secondary antibody (D-i). EM images of an infected cell at higher magnification (D-ii). Immunostaining for ubiquitin and Scribble in CHIKV infected HEK293T cells (E). Data represents mean ± SEM of two biological replicates.

    Article Snippet: The cDNA sequences of CHIKV 6K and TF (Supplementary Figure S1) were cloned in the pEGFPC1 vector (Clontech) to generate fusion proteins tagged with the Enhanced Green Fluorescent Protein (EGFP) at the N-terminus.

    Techniques: Infection, Western Blot, Immunostaining, Expressing, Transfection, Plasmid Preparation, Electron Microscopy, Imaging, Labeling