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srrgeco1 expression plasmid  (Addgene inc)


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    Structured Review

    Addgene inc srrgeco1 expression plasmid
    Srrgeco1 Expression Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfpc1+expression+vector/pEGFPC1-FLAG-Synaptojanin+1-170+(Plasmid+%2322294)/pmc11708886-262-0-6
    Average 93 stars, based on 3 article reviews
    srrgeco1 expression plasmid - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Recombinant:

    Article Title: Correlative single-molecule and structured illumination microscopy of fast dynamics at the plasma membrane.
    Article Snippet: First-order diffractions are imaged to the back focal plane of the objective by two relay lenses (L13; #47-647, Edmund optics, andL14;ACT508-400-A, Thorlabs).Weused two identical 3mm polychroic mirrors (DM3 and DM4; zt405/488/561/640rpc, Chroma, 3mm) to minimize wave front distortions and to guarantee azimuthal polarization state after reflection. .. The first dichroic mirror (DM3) is positioned such that the s- and p-axes are switched compared to the Table 1 | Recombinant DNA Denomination Plasmid name Source tdALFAnb pET-21a tdALFAnb-H6 this study (Addgene #222944) mXFP-TpoR pSems leader mXFP-TpoR Wilmes et al.41 ALFA-mXFP-TpoR pSems leader ALFA-mXFP-TpoR this study (Addgene #222945) Jak2-tdmCherry pSems JAK2-tdmCherry this study (Addgene #222946) Jak2ΔTK-mEGFP pSems JAK2 (1-827)-mEGFP Wilmes et al.41 LifeAct-HaloTag pSems LifeAct-HaloTag Wilmes et al.101 LifeAct-StayGold (LifeAct-SG) pSems LifeAct-StayGold • StayGold from Addgene #185823102 this study (Addgene #222947) StayGold-2xFYVE (SG-2xFYVE) pSems StayGold tdmFYVE • StayGold from Addgene #185823102 • 2xFYVE from Addgene #140047103 this study (Addgene #222948) TOM20-mEGFP pSems TOM20-meGFP Appelhans et al.104 TOM20-HaloTag pSems TOM20-HaloTag Appelhans et al.104 farnesyl-GFP pSems farnesyl-GFP this study (Addgene #222949) Nature Communications | (2024) 15:5813 11 second dichroic mirror (DM4) located in the filter turret of the microscope51,87. .. The back focal plane could also be imaged by an inspection CMOS camera (BFP Cam; Alvium 1800 U-319m, Allied Vision) by using a flip mirror (FM) and two relay lenses (L15 and L16; AC254-200-A and LA1229-A, Thorlabs).

    Plasmid Preparation:

    Article Title: Correlative single-molecule and structured illumination microscopy of fast dynamics at the plasma membrane.
    Article Snippet: First-order diffractions are imaged to the back focal plane of the objective by two relay lenses (L13; #47-647, Edmund optics, andL14;ACT508-400-A, Thorlabs).Weused two identical 3mm polychroic mirrors (DM3 and DM4; zt405/488/561/640rpc, Chroma, 3mm) to minimize wave front distortions and to guarantee azimuthal polarization state after reflection. .. The first dichroic mirror (DM3) is positioned such that the s- and p-axes are switched compared to the Table 1 | Recombinant DNA Denomination Plasmid name Source tdALFAnb pET-21a tdALFAnb-H6 this study (Addgene #222944) mXFP-TpoR pSems leader mXFP-TpoR Wilmes et al.41 ALFA-mXFP-TpoR pSems leader ALFA-mXFP-TpoR this study (Addgene #222945) Jak2-tdmCherry pSems JAK2-tdmCherry this study (Addgene #222946) Jak2ΔTK-mEGFP pSems JAK2 (1-827)-mEGFP Wilmes et al.41 LifeAct-HaloTag pSems LifeAct-HaloTag Wilmes et al.101 LifeAct-StayGold (LifeAct-SG) pSems LifeAct-StayGold • StayGold from Addgene #185823102 this study (Addgene #222947) StayGold-2xFYVE (SG-2xFYVE) pSems StayGold tdmFYVE • StayGold from Addgene #185823102 • 2xFYVE from Addgene #140047103 this study (Addgene #222948) TOM20-mEGFP pSems TOM20-meGFP Appelhans et al.104 TOM20-HaloTag pSems TOM20-HaloTag Appelhans et al.104 farnesyl-GFP pSems farnesyl-GFP this study (Addgene #222949) Nature Communications | (2024) 15:5813 11 second dichroic mirror (DM4) located in the filter turret of the microscope51,87. .. The back focal plane could also be imaged by an inspection CMOS camera (BFP Cam; Alvium 1800 U-319m, Allied Vision) by using a flip mirror (FM) and two relay lenses (L15 and L16; AC254-200-A and LA1229-A, Thorlabs).

    Article Title: Electrical signaling and coordinated behavior in the closest relative of animals
    Article Snippet: .. SrRGECO1 expression plasmid is deposited at Addgene (ID 222940). ..

    Expressing:

    Article Title: Electrical signaling and coordinated behavior in the closest relative of animals
    Article Snippet: .. SrRGECO1 expression plasmid is deposited at Addgene (ID 222940). ..

    other:

    Article Title: Correlative single-molecule and structured illumination microscopy of fast dynamics at the plasma membrane
    Article Snippet: Jak2-tdmCherry , pSems JAK2-tdmCherry , this study (Addgene #222946).

    Article Title: Correlative single-molecule and structured illumination microscopy of fast dynamics at the plasma membrane
    Article Snippet: All plasmids from this study were deposited at Addgene (https://www.addgene.org/, IDs 222944-222949).

    Article Title: Correlative single-molecule and structured illumination microscopy of fast dynamics at the plasma membrane
    Article Snippet: ALFA-mXFP-TpoR , pSems leader ALFA-mXFP-TpoR , this study (Addgene #222945).



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    Figure 2: Co-immunoprecipitation experiments in HEK293 cells transfected with (A) pEF-BOS-SNAP25-FLAG alone or together with <t>pEGFPC1-TrkB.</t> (B) pEF-BOS-VAMP2-FLAG alone or together with pEGFPC1-TrkB. (C) pEF-BOS-SNAP25-FLAG, pEF-BOS- VAMP2-FLAG and TrkA-HA. (D) pEF-BOS-SNAP25-FLAG, pEF-BOS-VAMP2-FLAG and TrkC-myc. No co-immunoprecipitation was observed between proteins analyzed with anti α-GFP, anti-myc, anti-FLAG, or anti-HA antibodies. Two transfections in HEK cells per condition were run in parallel for each experiment and three experiments were done. Arrows indicate specific bands.
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    Image Search Results


    Figure 2: Co-immunoprecipitation experiments in HEK293 cells transfected with (A) pEF-BOS-SNAP25-FLAG alone or together with pEGFPC1-TrkB. (B) pEF-BOS-VAMP2-FLAG alone or together with pEGFPC1-TrkB. (C) pEF-BOS-SNAP25-FLAG, pEF-BOS- VAMP2-FLAG and TrkA-HA. (D) pEF-BOS-SNAP25-FLAG, pEF-BOS-VAMP2-FLAG and TrkC-myc. No co-immunoprecipitation was observed between proteins analyzed with anti α-GFP, anti-myc, anti-FLAG, or anti-HA antibodies. Two transfections in HEK cells per condition were run in parallel for each experiment and three experiments were done. Arrows indicate specific bands.

    Journal: Oncotarget

    Article Title: Syntaxin-1/TI-VAMP SNAREs interact with Trk receptors and are required for neurotrophin-dependent outgrowth.

    doi: 10.18632/oncotarget.26307

    Figure Lengend Snippet: Figure 2: Co-immunoprecipitation experiments in HEK293 cells transfected with (A) pEF-BOS-SNAP25-FLAG alone or together with pEGFPC1-TrkB. (B) pEF-BOS-VAMP2-FLAG alone or together with pEGFPC1-TrkB. (C) pEF-BOS-SNAP25-FLAG, pEF-BOS- VAMP2-FLAG and TrkA-HA. (D) pEF-BOS-SNAP25-FLAG, pEF-BOS-VAMP2-FLAG and TrkC-myc. No co-immunoprecipitation was observed between proteins analyzed with anti α-GFP, anti-myc, anti-FLAG, or anti-HA antibodies. Two transfections in HEK cells per condition were run in parallel for each experiment and three experiments were done. Arrows indicate specific bands.

    Article Snippet: Full-length Sytx1A or truncated DNAs (Sytx1 ACYT, Sytx1AHabc, Sytx1AH3TM, Sytx1AH3, and Sytx1ATM) were cloned into EcoRI sites in pEGFPC1 fusion mammalian protein expression vector (Clontech), as described [18].

    Techniques: Immunoprecipitation, Transfection

    Journal: eLife

    Article Title: SAFB regulates hippocampal stem cell fate by targeting Drosha to destabilize Nfib mRNA

    doi: 10.7554/eLife.74940

    Figure Lengend Snippet:

    Article Snippet: Recombinant DNA reagent , pEGFPC1-6XHis- FLKSRP (plasmid) , , RRID: Addgene_23001 , pEGFPC1 expression vector.

    Techniques: Protease Inhibitor, Recombinant, Clone Assay, Bicinchoninic Acid Protein Assay, Mutagenesis, Transfection, Sequencing, esiRNA, Blocking Assay, Plasmid Preparation, Expressing, Software