Journal: Oncotarget
Article Title: Syntaxin-1/TI-VAMP SNAREs interact with Trk receptors and are required for neurotrophin-dependent outgrowth.
doi: 10.18632/oncotarget.26307
Figure Lengend Snippet: Figure 2: Co-immunoprecipitation experiments in HEK293 cells transfected with (A) pEF-BOS-SNAP25-FLAG alone or together with pEGFPC1-TrkB. (B) pEF-BOS-VAMP2-FLAG alone or together with pEGFPC1-TrkB. (C) pEF-BOS-SNAP25-FLAG, pEF-BOS- VAMP2-FLAG and TrkA-HA. (D) pEF-BOS-SNAP25-FLAG, pEF-BOS-VAMP2-FLAG and TrkC-myc. No co-immunoprecipitation was observed between proteins analyzed with anti α-GFP, anti-myc, anti-FLAG, or anti-HA antibodies. Two transfections in HEK cells per condition were run in parallel for each experiment and three experiments were done. Arrows indicate specific bands.
Article Snippet: Full-length Sytx1A or truncated DNAs (Sytx1 ACYT, Sytx1AHabc, Sytx1AH3TM, Sytx1AH3, and Sytx1ATM) were cloned into EcoRI sites in pEGFPC1 fusion mammalian protein expression vector (Clontech), as described [18].
Techniques: Immunoprecipitation, Transfection