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pcr direct sequencing method abi 3730xl  (Thermo Fisher)


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    Structured Review

    Thermo Fisher pcr direct sequencing method abi 3730xl
    Pcr Direct Sequencing Method Abi 3730xl, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcr-direct+sequence+method/pmc09289245-91-13-17
    Average 90 stars, based on 1 article reviews
    pcr direct sequencing method abi 3730xl - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Multiplex Assay:

    Article Title: Comparative genetic analysis of blood and semen samples in sperm donors from Hunan, China.
    Article Snippet: .. For gene copy number detection of SMN1/2, we used multiplex fluorescent PcR and capillary electrophoresis (aBi 3730Xl), and lR-PcR combined with NGs was used to detect and validate 15 common SMN1 mutations in the chinese population. .. the sequencing data were aligned to GRch37/hg19 reference genome (map reads to reference) by sentieon® software (san Jose, ca, Usa). and then marking duplicates, base quality score recalibration (BQsR) and variant calling were processed.

    Electrophoresis:

    Article Title: Comparative genetic analysis of blood and semen samples in sperm donors from Hunan, China.
    Article Snippet: .. For gene copy number detection of SMN1/2, we used multiplex fluorescent PcR and capillary electrophoresis (aBi 3730Xl), and lR-PcR combined with NGs was used to detect and validate 15 common SMN1 mutations in the chinese population. .. the sequencing data were aligned to GRch37/hg19 reference genome (map reads to reference) by sentieon® software (san Jose, ca, Usa). and then marking duplicates, base quality score recalibration (BQsR) and variant calling were processed.

    Article Title: Genetic diversity, population structure and quality-trait associations in Blumea balsamifera revealed by EST-SSR markers
    Article Snippet: .. Prior to fluorescence capillary electrophoresis on a ABI 3730XL sequencer, 10 μL of Hi-Di formamide and 0.5 μL of an internal standard were mixed thoroughly, and 1 μL of the diluted PCR product was added. ..

    Polymerase Chain Reaction:

    Article Title: Molecular genotyping of multi-system rare blood types in foreign blood donors based on DNA sequencing and its clinical significance
    Article Snippet: .. PCR products were verified by 1.5% agarose gel electrophoresis before being sent to BGI for bidirectional sequencing using ABI 3730XL sequencer and BigDye Terminator v3.1 kit. ..

    Article Title: Green and Chemical Synthesis of Copper Oxide Nanoparticles for Antifungal Protection and Plant Growth Enhancement of Chickpea via Nanopriming.
    Article Snippet: This study reports the synthesis of copper oxide nanoparticles (CuO NPs) using chemical and green methods utilizing pineapple waste, including fresh peels, dried peels, and pineapple pomace from two different copper precursors, targeting antifungal activity and plant growth enhancement.. Phytochemical analysis confirmed the presence of bioactive compounds with dry peel extract proving to be most effective for green synthesis.. CuO NP formation was evidenced by color change and confirmed by UV−vis, Fourier transform infrared (FTIR), X-ray diffraction (XRD), and transmission electron microscopy (TEM) analysis, revealing particle sizes under 50 nm.

    Article Title: Fungal Biosystems for the Fabrication of Copper Nanoparticles: An Effective Approach for the Treatment of Bacterial Biofilms.
    Article Snippet: Multidrug-resistant bacterial infections present a serious global public health threat.. This threat is exacerbated by biofilm-forming bacteria that have greater intrinsic resistance to various classes of currently available antibiotics.. This study reports the fabrication of copper nanoparticles (CuNPs) by using a cell-free lysate of Aspergillus niger (A. niger).

    Article Title: Genetic diversity, population structure and quality-trait associations in Blumea balsamifera revealed by EST-SSR markers
    Article Snippet: .. Prior to fluorescence capillary electrophoresis on a ABI 3730XL sequencer, 10 μL of Hi-Di formamide and 0.5 μL of an internal standard were mixed thoroughly, and 1 μL of the diluted PCR product was added. ..

    Article Title: Novel DNA Barcoding and Multiplex PCR Strategy for the Molecular Identification and Mycotoxin Gene Detection of Fusarium spp. in Maize from Bulgaria
    Article Snippet: The obtained PCR products were purified with a GeneMATRIX Short DNA Clean-Up Purification Kit (Cat. No. E3515, EURx Ltd., Gdansk, Poland) and sequenced in both directions by a PlateSeq kit (Eurofins Genomics Ebersberg, Gdansk, Germany). .. For all PCR products, Sanger Sequencing cycle sequencing technology (dideoxy chain termination/cycle sequencing) on an ABI 3730XL sequencing machine was used. ..

    Agarose Gel Electrophoresis:

    Article Title: Molecular genotyping of multi-system rare blood types in foreign blood donors based on DNA sequencing and its clinical significance
    Article Snippet: .. PCR products were verified by 1.5% agarose gel electrophoresis before being sent to BGI for bidirectional sequencing using ABI 3730XL sequencer and BigDye Terminator v3.1 kit. ..

    Sequencing:

    Article Title: Molecular genotyping of multi-system rare blood types in foreign blood donors based on DNA sequencing and its clinical significance
    Article Snippet: .. PCR products were verified by 1.5% agarose gel electrophoresis before being sent to BGI for bidirectional sequencing using ABI 3730XL sequencer and BigDye Terminator v3.1 kit. ..

    Article Title: Green and Chemical Synthesis of Copper Oxide Nanoparticles for Antifungal Protection and Plant Growth Enhancement of Chickpea via Nanopriming.
    Article Snippet: This study reports the synthesis of copper oxide nanoparticles (CuO NPs) using chemical and green methods utilizing pineapple waste, including fresh peels, dried peels, and pineapple pomace from two different copper precursors, targeting antifungal activity and plant growth enhancement.. Phytochemical analysis confirmed the presence of bioactive compounds with dry peel extract proving to be most effective for green synthesis.. CuO NP formation was evidenced by color change and confirmed by UV−vis, Fourier transform infrared (FTIR), X-ray diffraction (XRD), and transmission electron microscopy (TEM) analysis, revealing particle sizes under 50 nm.

    Article Title: Fungal Biosystems for the Fabrication of Copper Nanoparticles: An Effective Approach for the Treatment of Bacterial Biofilms.
    Article Snippet: Multidrug-resistant bacterial infections present a serious global public health threat.. This threat is exacerbated by biofilm-forming bacteria that have greater intrinsic resistance to various classes of currently available antibiotics.. This study reports the fabrication of copper nanoparticles (CuNPs) by using a cell-free lysate of Aspergillus niger (A. niger).

    Article Title: Novel DNA Barcoding and Multiplex PCR Strategy for the Molecular Identification and Mycotoxin Gene Detection of Fusarium spp. in Maize from Bulgaria
    Article Snippet: The obtained PCR products were purified with a GeneMATRIX Short DNA Clean-Up Purification Kit (Cat. No. E3515, EURx Ltd., Gdansk, Poland) and sequenced in both directions by a PlateSeq kit (Eurofins Genomics Ebersberg, Gdansk, Germany). .. For all PCR products, Sanger Sequencing cycle sequencing technology (dideoxy chain termination/cycle sequencing) on an ABI 3730XL sequencing machine was used. ..

    DNA Sequencing:

    Article Title: Green and Chemical Synthesis of Copper Oxide Nanoparticles for Antifungal Protection and Plant Growth Enhancement of Chickpea via Nanopriming.
    Article Snippet: This study reports the synthesis of copper oxide nanoparticles (CuO NPs) using chemical and green methods utilizing pineapple waste, including fresh peels, dried peels, and pineapple pomace from two different copper precursors, targeting antifungal activity and plant growth enhancement.. Phytochemical analysis confirmed the presence of bioactive compounds with dry peel extract proving to be most effective for green synthesis.. CuO NP formation was evidenced by color change and confirmed by UV−vis, Fourier transform infrared (FTIR), X-ray diffraction (XRD), and transmission electron microscopy (TEM) analysis, revealing particle sizes under 50 nm.

    Amplification:

    Article Title: Green and Chemical Synthesis of Copper Oxide Nanoparticles for Antifungal Protection and Plant Growth Enhancement of Chickpea via Nanopriming.
    Article Snippet: This study reports the synthesis of copper oxide nanoparticles (CuO NPs) using chemical and green methods utilizing pineapple waste, including fresh peels, dried peels, and pineapple pomace from two different copper precursors, targeting antifungal activity and plant growth enhancement.. Phytochemical analysis confirmed the presence of bioactive compounds with dry peel extract proving to be most effective for green synthesis.. CuO NP formation was evidenced by color change and confirmed by UV−vis, Fourier transform infrared (FTIR), X-ray diffraction (XRD), and transmission electron microscopy (TEM) analysis, revealing particle sizes under 50 nm.

    Article Title: Fungal Biosystems for the Fabrication of Copper Nanoparticles: An Effective Approach for the Treatment of Bacterial Biofilms.
    Article Snippet: Multidrug-resistant bacterial infections present a serious global public health threat.. This threat is exacerbated by biofilm-forming bacteria that have greater intrinsic resistance to various classes of currently available antibiotics.. This study reports the fabrication of copper nanoparticles (CuNPs) by using a cell-free lysate of Aspergillus niger (A. niger).

    Purification:

    Article Title: Heat Stress Response in Ruditapes Decussatus : Transcriptional Regulation of Key Pathways and Novel SNPs in Antioxidant Genes
    Article Snippet: All accurately amplified PCR products were cleaned using the NucleoSpin Gel and PCR Clean‐up kit (Macherey Nagel, Düren, Germany). .. The purified samples were sequenced in both directions in an ABI 3730xl automatic sequencer. .. Sequencing quality was evaluated by visual examination of the derived chromatograms using Finch TV 1.4.0 (Geospiza, Seattle, WA, USA) and BioEdit (Hall ).

    other:

    Article Title: Neuroprotective and probiotic potential of Lactiplantibacillus plantarum AM2 in cognitive impairment.
    Article Snippet: The isolate that showed the highest production of ACh underwent molecular sequencing (ABI PRISM 3730xl DNA sequencer), biochemical identification (Biologsystem VITEK 2 Version 7.01), and matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) proteomic identification (SAI, LT2 +, UK).

    Fluorescence:

    Article Title: Genetic diversity, population structure and quality-trait associations in Blumea balsamifera revealed by EST-SSR markers
    Article Snippet: .. Prior to fluorescence capillary electrophoresis on a ABI 3730XL sequencer, 10 μL of Hi-Di formamide and 0.5 μL of an internal standard were mixed thoroughly, and 1 μL of the diluted PCR product was added. ..



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    Image Search Results


    Comparative analysis of aligned sequencing reads on the human BCL2L12 gene, derived from the presented 5′ RACE-seq approach and publicly available Direct RNA sequencing datasets. A Visualization of the aligned sequencing reads derived from the presented 5′ RACE-seq approach. B Visualization of the aligned sequencing reads derived from publicly available Direct RNA sequencing datasets deposited on the Sequence Read Archive database (SRA). C Indicative raw nanopore sequencing reads from the generated FASTQ file of the presented 5′ RACE-seq approach, highlighting the incorporation of the drTSO specifically to the 5′ end of each target mRNA transcript

    Journal: BMC Genomics

    Article Title: A versatile 5′ RACE-Seq methodology for the accurate identification of the 5′ termini of mRNAs

    doi: 10.1186/s12864-022-08386-y

    Figure Lengend Snippet: Comparative analysis of aligned sequencing reads on the human BCL2L12 gene, derived from the presented 5′ RACE-seq approach and publicly available Direct RNA sequencing datasets. A Visualization of the aligned sequencing reads derived from the presented 5′ RACE-seq approach. B Visualization of the aligned sequencing reads derived from publicly available Direct RNA sequencing datasets deposited on the Sequence Read Archive database (SRA). C Indicative raw nanopore sequencing reads from the generated FASTQ file of the presented 5′ RACE-seq approach, highlighting the incorporation of the drTSO specifically to the 5′ end of each target mRNA transcript

    Article Snippet: Our results led to the accurate identification of the 5′ UTRs of the BCL2L12 gene, being in accordance with the 5′ ends provided by both direct RNA and PCR-cDNA sequencing method from Oxford Nanopore Technologies.

    Techniques: Sequencing, Derivative Assay, RNA Sequencing, Nanopore Sequencing, Generated

    Template-switching oligonucleotides (TSOs) and 5′ RACE primers used in the present work

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    Figure Lengend Snippet: Template-switching oligonucleotides (TSOs) and 5′ RACE primers used in the present work

    Article Snippet: Our results led to the accurate identification of the 5′ UTRs of the BCL2L12 gene, being in accordance with the 5′ ends provided by both direct RNA and PCR-cDNA sequencing method from Oxford Nanopore Technologies.

    Techniques: Sequencing, Modification