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deep vent dna polymerase  (New England Biolabs)


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    Structured Review

    New England Biolabs deep vent dna polymerase
    Deep Vent Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 3337 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcr-based+pyrosequencing+method/Deep+Vent+DNA+Polymerase/custom%40m0258%4019820095
    Average 96 stars, based on 3337 article reviews
    deep vent dna polymerase - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    other:

    Article Title: Purifying and polymerizing 3′-blocked nucleotides
    Article Snippet: A nonlimiting example of a polishing polymerase is a “thermostable” polymerase, which refers to a polymerase that may function well at relatively high temperatures, e.g., at about 30° C. to about 100° C., or at about 40° C. to about 80° C., or at about 50° C. to about 70° C. Examples of thermostable polymerases include the Pyrococcus sp. (strain GB-D) DNA polymerase with trade name DEEP VENT® DNA Polymerase (example working temperature 75° C.), Thermus aquaticus DNA polymerase I (Taq polymerase) (example working temperature 75° C.), Bst (example working temperature 65° C.), Sulfolobus DNA Polymerase IV (example working temperature 55° C.), and Pfu (Phusion) (example working temperature 75° C.), all of which are commercially available from New England Biolabs, Inc. (Ipswich, MA).

    Article Title: Methods for the detection of a nucleic acid
    Article Snippet: Non-limiting examples of a proofreading DNA polymerase that can be used in methods as provided herein include PHUSION HOT START IITM DNA polymerase (ThermoFisher Scientific, cat. #F549L); Q5® HOT START HIGH-FIDELITYTM DNA polymerase (New England Biolabs, cat. #M0493L); DEEP VENT® DNA polymerase (New England Biolabs, cat. #M0258L) and PLATINUM SUPERFI IITM DNA polymerase (ThermoFisher Scientific, cat. #12361050).

    Article Title: Quantifying replication fidelity of unnatural base pairs using nanopore sequencing
    Article Snippet: Thermostable polymerases Q5 (M0491L), Phusion (M0530S), DeepVent (M0258S), DeepVent (exo-) (M0259S), and OneTaq (M0480S) were purchased from New England Biolabs (Ipswich, MA).

    Article Title: Methods for the detection of a nucleic acid
    Article Snippet: Non-limiting examples of a proofreading DNA polymerase that can be used in methods as provided herein include PHUSION HOT START IITM DNA polymerase (ThermoFisher Scientific, cat. #F549L); Q5® HOT START HIGH-FIDELITYTM DNA polymerase (New England Biolabs, cat. #M0493L); DEEP VENT® DNA Polymerase (New England Biolabs, cat. #M0258L) and PLATINUM SUPERFI IITM DNA polymerase (ThermoFisher Scientific, cat. #12361050).

    Article Title: Methods for the detection of a nucleic acid
    Article Snippet: Non-limiting examples of a proofreading DNA polymerase that can be used in methods as provided herein include PHUSION HOT START IITM DNA polymerase (ThermoFisher Scientific, cat. #F549L); Q5® Hot Start High-Fidelity DNA polymerase (New England Biolabs, cat. #M0493L); Deep Vent® DNA polymerase (New England Biolabs, cat. #M0258L) and PLATINUM SUPERFI IITM DNA polymerase (ThermoFisher Scientific, cat. #12361050).

    Reverse Transcription:

    Article Title: Isolation and detection of DNA from plasma
    Article Snippet: .. Polymerases include but are not limited to T7 DNA polymerase, T3 DNA polymerase, T4 DNA polymerase, T7 RNA polymerase, T3 RNA polymerase, SP6 RNA polymerase, DNA polymerase 1, Klenow fragment, Thermophilus aquaticus DNA polymerase, Tth DNA polymerase, Vent DNA polymerase (New England Biolabs), Deep Vent DNA polymerase (New England Biolabs), Bst DNA Polymerase Large Fragment, Stoeffel Fragment, 9° N DNA Polymerase, Pfu DNA Polymerase, Tfl DNA Polymerase, RepliPHI Phi29 Polymerase, Tli DNA polymerase, eukaryotic DNA polymerase beta, telomerase, Therminator polymerase (New England Biolabs), KOD HiFi DNA polymerase (Novagen), KOD1 DNA polymerase, Q-beta replicase, terminal transferase, AMV reverse transcriptase, M-MLV reverse transcriptase, Phi6 reverse transcriptase, HIV-1 reverse transcriptase, novel polymerases discovered by bioprospecting, and polymerases cited in US 2007/0048748, U.S. Pat. ..

    Recombinant:

    Article Title: A simple self-mediated nucleic acid elongation-driven amplification strategy for sensitive detection of terminal deoxynucleotidyl transferase activity
    Article Snippet: Terminal deoxynucleotidyl transferase (TdT) plays a pivotal role in the human immune system.. Its increased activity in leukemic cells renders it a vital biomarker for leukemia.. However, current TdT assays usually involve elaborate protocols and high costs.

    Purification:

    Article Title: Purifying and polymerizing 3′-blocked nucleotides
    Article Snippet: .. More specifically, the next data points to the right (“Vent polishing 60 C”) correspond to polymerization of 3′-blocked nucleotides that had been heated as indicated above and were purified using the Deep Vent polymerase at 60° C. (DEEP VENT® DNA Polymerase (New England Biolabs, Inc., Ipswich, MA)); these 3′-blocked nucleotides were found to have a normalized phasing 921 of about 0.12% and a normalized prephasing 922 of about 0.15%. ..



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