pcr buffer (Genesee Scientific)

Genesee Scientific is a verified supplier

Genesee Scientific manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Genesee Scientific pcr buffer
Pcr Buffer, supplied by Genesee Scientific, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcr+buffer/PCR+Buffer+I%2C+Mg+Free/pmc07593134-83-10-31
Average 93 stars, based on 10 article reviews

pcr buffer - by Bioz Stars, 2026-10

93/100 stars

Images

Polymerase Chain Reaction:

Article Title: Thrips tabaci Population Genetic Structure and Polyploidy in Relation to Competency as a Vector of Tomato Spotted Wilt Virus
Article Snippet: .. PCRs were carried out in 12 μl total volumes, each containing 1× PCR buffer, 1.75 mM MgCl 2 , 100 mM dNTPs, ∼20 ng DNA template, 0.5 U Apex Taq DNA polymerase (Genesee Scientific, San Diego, CA), and ddH 2 O to 12 μl. .. Primer concentration varied between 0.10 and 0.15 μM ( ) with the forward primer end-labeled with an M13F-29/IRD700 or 800 IRDye tag (Li-Cor Inc., Lincoln, NE).

Article Title: New insights on facultative parthenogenesis in pythons
Article Snippet: .. Polymerase chain reactions (PCR) were carried out in 12-μL total volumes, each containing 1 × PCR buffer, 1.75 mM MgCl2, 100 mM dNTPs, approximately 20 ng of DNA template, 1 pmol of primer, 0.5 U of Apex Taq DNA polymerase (Genesee Scientific), and PCR-grade H2O to 12 μL. .. The forward primer of each pair was endlabelled with a M13F-29 IRDye tag (LI-COR Biosciences).

Article Title: Extensive Mitochondrial Heteroplasmy in Natural Populations of a Resurging Human Pest, the Bed Bug (Hemiptera: Cimicidae)
Article Snippet: .. PCR was performed in 18-μl volumes containing: 1× PCR Buffer, 2 mM MgCl 2 , 100 mM dNTPs, 2.5 μM of each primer, 0.5 U Taq DNA polymerase (Apex, Genesee Scientific, San Diego), ∼50 ng of DNA template, and ddH 2 O to make 18 μl. ..

Article Title: An eDNA diagnostic test to detect a rare, secretive marsh bird
Article Snippet: We extracted genomic DNA using a DNeasy Blood and Tissue Kit (Qiagen). .. We amplified 2 μL of DNA template in 25 μL reactions with 11.5 μL nuclease free water, 2.5 μL Apex 10x Mg-free PCR buffer, 1.5 mM MgCl2, 0.2 mM dNTPs, 0.5 μM of each primer (Laterallus COI or Rail COI), and 1.25 Units Taq Polymerase (Apex, Genesee Scientific). ..

Article Title: Disentangling the mechanisms of mate choice in a captive koala population
Article Snippet: .. PCRs were carried out in a 10 μL reaction volume containing 1× PCR buffer, 2.5 mM MgCl, 0.2 mM dNTPs (Apex; Genesee Scientific, San Deigo, CA, USA), 0.2 μM forward primer, 0.6 μM reverse primer and 0.5 U AmpliTaq Gold (Applied Biosystems, Waltham, MA, USA) (see for thermocycling conditions). .. Samples were genotyped on an ABI 3130xl using an internal GeneScan 500 ROX size standard and alleles were automatically called then manually checked using GeneMapper (ABI).

Article Title: An eDNA diagnostic test to detect a rare, secretive marsh bird
Article Snippet: We tested the primers for cross-species amplification with genomic DNA from blood or tissue samples of sympatric rail species (king rail, clapper rail, yellow rail, common gallinule, Virginia rail, sora) using traditional PCR (BioRad T100 thermocycler). .. The 10 μL reactions included 5.6 μL nuclease-free water (Fisher Scientific), 1X Apex Mg-free PCR buffer, 1.5 mM MgCl2, 0.2 mM dNTPs, 0.5 μM of each BLRA COI2 primer, 0.5 Units Taq Polymerase (Apex, Genesee Scientific), and 2 μL genomic DNA template. ..

Article Title: Temporal Occurrence and Niche Preferences of Phytophthora spp. Causing Brown Rot of Citrus in the Central Valley of California
Article Snippet: .. Each 25-μl reaction contained 1 μl of DNA extract (10 to 20 ng DNA), 2.5 μl of 10× PCR buffer containing 150 μM MgCl2 (Apex; Genesee Scientific, San Diego, CA), 200 μM dNTP, 2.5 μg of bovine serum albumin (BSA), 0.4 μM each primer, and 1.25 U of Taq DNApolymerase (Apex; Genesee Scientific). .. PCR was performed using a Programmed Thermal Controller (PTC-100; MJ Research, Inc., Watertown, MA) with conditions as follows: initial denaturation at 95 C for 2 min; 35 cycles of denaturation at 95 C for 30 s, annealing at 55 C for ITS primers or 58 C for cox primers for 30 s, and extension at 72 C for 30 s; and a final extension at 72 C for 2 min.

Article Title: Reproductive compatibility among populations and host‐associated lineages of the common bed bug ( Cimex lectularius L.)
Article Snippet: .. PCRs were performed in 20 μl volumes each containing 1× PCR buffer, 2 mM MgCl 2 , 100 mM dNTPs, 2.5 mM of each primer, 0.5 U Taq DNA polymerase (Apex, Genesee Scientific), 50 ng of DNA template, and ddH 2 O to make 20 μl. ..

Review

pcr buffer (Genesee Scientific)

Structured Review

Images

Related Articles

Polymerase Chain Reaction:

Amplification:

Similar Products

Image Search Results