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2x pcr buffer  (Jumpstart Fertility)

 
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    Structured Review

    Jumpstart Fertility 2x pcr buffer
    2x Pcr Buffer, supplied by Jumpstart Fertility, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcr+buffer/2x+buffer+pcr/pm42086800-74-7-10
    Average 86 stars, based on 1 article reviews
    2x pcr buffer - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Amplification:

    Article Title: Investigating the genetic potential of Ashwagandha (Withania somnifera L. Dunal) germplasm through SCoT and EST-SSR molecular markers.
    Article Snippet: .. Amplification was performed using 5 μl of 2X PCR buffer (Jumpstart ready mix-P1107) with 1μL of 25 ng of template DNA and 1 μL of primer in a reaction volume of 10 μL and conducted using a thermal cycler (Biorad) for an initial denaturation at 94 °C for 3 minutes, followed by 40 cycles consisting of denaturation at 94 °C for 1 minute, annealing at 50 °C- 57 °C for 1 minute, and extension at 72 °C for 2 minutes with final extension for 5 min at 72 °C. .. Agarose gel electrophoresis was used to separate the PCR products on a 1.5% agarose gel for 2 hours at 80 V, and the Gel Documentation system (Eppendorf, USA) was used to capture images under UV light.

    Polymerase Chain Reaction:

    Article Title: Investigating the genetic potential of Ashwagandha (Withania somnifera L. Dunal) germplasm through SCoT and EST-SSR molecular markers.
    Article Snippet: .. Amplification was performed using 5 μl of 2X PCR buffer (Jumpstart ready mix-P1107) with 1μL of 25 ng of template DNA and 1 μL of primer in a reaction volume of 10 μL and conducted using a thermal cycler (Biorad) for an initial denaturation at 94 °C for 3 minutes, followed by 40 cycles consisting of denaturation at 94 °C for 1 minute, annealing at 50 °C- 57 °C for 1 minute, and extension at 72 °C for 2 minutes with final extension for 5 min at 72 °C. .. Agarose gel electrophoresis was used to separate the PCR products on a 1.5% agarose gel for 2 hours at 80 V, and the Gel Documentation system (Eppendorf, USA) was used to capture images under UV light.



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