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Valiant Co Ltd taq dna polymerase
Taq Dna Polymerase, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 895 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcr+buffer/Taq+DNA+polymerase/pm18783356-63-31-34
Average 94 stars, based on 895 article reviews
taq dna polymerase - by Bioz Stars, 2026-09
94/100 stars

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Related Articles

Modification:

Article Title: Genetic diversity and evolution of endogenous pararetroviruses across Solanaceae : How farming systems drive dynamic tomato EPRVS changes under salt stress
Article Snippet: 500ng of DNA was treated with sodium bisulfite ( ) using the Methylation Kit (Zymo, USA). .. Modified DNA was amplified by PCR using IGR1/2primers and Taq DNA polymerase (MP Biomedicals, Europe). .. The amplicons were purified from agarose gel and cloned using the pGEM-TEasy vector system (Promega).

Amplification:

Article Title: Genetic diversity and evolution of endogenous pararetroviruses across Solanaceae : How farming systems drive dynamic tomato EPRVS changes under salt stress
Article Snippet: 500ng of DNA was treated with sodium bisulfite ( ) using the Methylation Kit (Zymo, USA). .. Modified DNA was amplified by PCR using IGR1/2primers and Taq DNA polymerase (MP Biomedicals, Europe). .. The amplicons were purified from agarose gel and cloned using the pGEM-TEasy vector system (Promega).

Article Title: Biombalance TM : A Specific Oligomeric Procyanidin-Rich Grape Seed Extract as Multifunctional Ingredient Integrating Antibacterial, Antioxidant, and Anti-Inflammatory Activities with Beneficial Gut–Brain Axis Modulation
Article Snippet: Approximately 100 mg of stool was homogenised in Tris-EDTA buffer (0.1 mM Tris, pH 8; 1 M EDTA; 1 mL buffer per 200 mg faeces), and lysozyme (300 mg/mL, Sigma, St. Louis, MO, USA) was added at a 1:100 ratio, followed by incubation at 37 °C for 1 h. DNA was then extracted from 300 μL of the mixture using the NucleoSpin ® Soil Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions, and quantified with a NanoDrop One spectrophotometer (Thermo Fisher Scientific, Wilmington, DE, USA) [ ]. .. The V3–V4 region of the 16S rRNA gene was amplified using primers 338F and 806R, with PCR performed using the MP Taq DNA Polymerase kit (MP Biomedicals, Illkirch, France) under standard cycling conditions [ ]. .. The PCR protocol involved an initial denaturation at 95 °C for 5 min, followed by 30 cycles comprising 30 s at 95 °C, 30 s at 52 °C for primer binding, and 45 s at 72 °C for strand elongation; the procedure concluded with a final elongation step at 72 °C for 2 min. Amplicons were sequenced on an Illumina MiSeq platform (Genotoul, Toulouse, France), and sequence quality was assessed using GALAXY FROGS 4.1.

Polymerase Chain Reaction:

Article Title: Genetic diversity and evolution of endogenous pararetroviruses across Solanaceae : How farming systems drive dynamic tomato EPRVS changes under salt stress
Article Snippet: 500ng of DNA was treated with sodium bisulfite ( ) using the Methylation Kit (Zymo, USA). .. Modified DNA was amplified by PCR using IGR1/2primers and Taq DNA polymerase (MP Biomedicals, Europe). .. The amplicons were purified from agarose gel and cloned using the pGEM-TEasy vector system (Promega).

Article Title: Repeated loss of function at HD mating-type genes and of recombination in anther-smut fungi.
Article Snippet: .. Boiling PCR was performed in a final volume of 30μL containing 3μL of fungal suspension, 15μL of 2X Buffer (containing a mix of dNTPs 5mM in addition of bovine serum albumin (BSA) 20mg/ml, W1 detergent 1%, home made 10X Buffer (Ammonium Sulfat 1M, Tris HCl 1M, MgCl26H2O 1M and sterile deionized H2O), 1.2μL of each forward and reverse primers (10μM), 0.15μL of Taq DNA Polymerase (5 U/μL, MP Biomedicals) and 9.8μL of sterile H2O. ..

Article Title: Biombalance™, an Oligomeric Procyanidins-Enriched Grape Seed Extract, Prevents Inflammation and Microbiota Dysbiosis in a Mice Colitis Model
Article Snippet: For Illumina microbiota sequencing, the 16S rRNA gene’s V3–V4 region was amplified using forward primer 338F (5′-ACTCCTACGGGAGGCAGCA-3′) and reverse primer 806R (5′-GGACTACHVGGGTWTCTAAT-3′), to prepare Illumina libraries. .. The PCR mixture, containing 10 ng of DNA, 10 μM of each primer and PCR-grade water in a final volume of 50 μL, was prepared using the MP Taq DNA Polymerase kit (Q-Bio DNA polymerase, MP Biomedicals, Illkirch-Graffenstaden, France). ..

Article Title: Genetic diversity and evolution of endogenous pararetroviruses across Solanaceae : How farming systems drive dynamic tomato EPRVS changes under salt stress
Article Snippet: .. PCR reaction was performed in a final volume of 25 μl containing 100 ng of each primer, 5 μl of 10X buffer, 1 μl of dNTP (10 mM each), 1.5 μl MgCl 2 (1.5 mM), 1U of Taq DNA polymerase (MP Biomedicals, Europe), 2 μl of extracted DNA, and sterile water. .. PCR amplifications were performed on a T professional TRIO system thermocycler (Biometra, Germany) using the following cycling program: One first denaturation step of 3 min at 94°C followed by 35 amplification cycles, each consisting of 30 sec at 94°C, 50 sec at 55°C, 1 min at 72°C.

Article Title: Yeast diversity in Brazilian artisanal cheeses: Unveiling technologically relevant species to improve traditional cheese production
Article Snippet: Although less studied than bacterial biota, the presence of yeast during the artisanal cheese’s production is of fundamental importance.. Yeasts can prevent the growth of undesirable species in the cheese’s core and surface and actively participate in the aromatic development of these products.. On the other hand, reintroducing the most abundant yeast species can help mitigate potential health risks related to artisanal production.

Article Title: Biombalance TM : A Specific Oligomeric Procyanidin-Rich Grape Seed Extract as Multifunctional Ingredient Integrating Antibacterial, Antioxidant, and Anti-Inflammatory Activities with Beneficial Gut–Brain Axis Modulation
Article Snippet: Approximately 100 mg of stool was homogenised in Tris-EDTA buffer (0.1 mM Tris, pH 8; 1 M EDTA; 1 mL buffer per 200 mg faeces), and lysozyme (300 mg/mL, Sigma, St. Louis, MO, USA) was added at a 1:100 ratio, followed by incubation at 37 °C for 1 h. DNA was then extracted from 300 μL of the mixture using the NucleoSpin ® Soil Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions, and quantified with a NanoDrop One spectrophotometer (Thermo Fisher Scientific, Wilmington, DE, USA) [ ]. .. The V3–V4 region of the 16S rRNA gene was amplified using primers 338F and 806R, with PCR performed using the MP Taq DNA Polymerase kit (MP Biomedicals, Illkirch, France) under standard cycling conditions [ ]. .. The PCR protocol involved an initial denaturation at 95 °C for 5 min, followed by 30 cycles comprising 30 s at 95 °C, 30 s at 52 °C for primer binding, and 45 s at 72 °C for strand elongation; the procedure concluded with a final elongation step at 72 °C for 2 min. Amplicons were sequenced on an Illumina MiSeq platform (Genotoul, Toulouse, France), and sequence quality was assessed using GALAXY FROGS 4.1.

Article Title: Repeated loss of function at HD mating-type genes and of recombination in anther-smut fungi
Article Snippet: .. Boiling PCR was performed in a final volume of 30 μL containing 3 μL of fungal suspension, 15 μL of 2X Buffer (containing a mix of dNTPs 5 mM in addition of bovine serum albumin (BSA) 20 mg/ml, W1 detergent 1%, home made 10X Buffer (Ammonium Sulfat 1 M, Tris HCl 1 M, MgCl 2 6H 2 O 1 M and sterile deionized H 2 O), 1.2 μL of each forward and reverse primers (10 μM), 0.15 μL of Taq DNA Polymerase (5 U/μL, MP Biomedicals) and 9.8 μL of sterile H 2 O. .. Boiling PCR was performed in a final volume of 30 μL containing 3 μL of fungal suspension, 15 μL of 2X Buffer (containing a mix of dNTPs 5 mM in addition of bovine serum albumin (BSA) 20 mg/ml, W1 detergent 1%, home made 10X Buffer (Ammonium Sulfat 1 M, Tris HCl 1 M, MgCl 2 6H 2 O 1 M and sterile deionized H 2 O), 1.2 μL of each forward and reverse primers (10 μM), 0.15 μL of Taq DNA Polymerase (5 U/μL, MP Biomedicals) and 9.8 μL of sterile H 2 O.

Article Title: Biombalance™, an Oligomeric Procyanidins-Enriched Grape Seed Extract, Prevents Inflammation and Microbiota Dysbiosis in a Mice Colitis Model.
Article Snippet: For Illumina microbiota sequencing, the 16S rRNA gene’s V3–V4 region was amplified using forward primer 338F (5′-ACTCCTACGGGAGGCAGCA3′) and reverse primer 806R (5′-GGACTACHVGGGTWTCTAAT-3′), to prepare Illumina libraries. .. The PCR mixture, containing 10 ng of DNA, 10 μM of each primer and PCR-grade water in a final volume of 50 μL, was prepared using the MP Taq DNA Polymerase kit (Q-Bio DNA polymerase, MP Biomedicals, Illkirch-Graffenstaden, France). ..

Suspension:

Article Title: Repeated loss of function at HD mating-type genes and of recombination in anther-smut fungi.
Article Snippet: .. Boiling PCR was performed in a final volume of 30μL containing 3μL of fungal suspension, 15μL of 2X Buffer (containing a mix of dNTPs 5mM in addition of bovine serum albumin (BSA) 20mg/ml, W1 detergent 1%, home made 10X Buffer (Ammonium Sulfat 1M, Tris HCl 1M, MgCl26H2O 1M and sterile deionized H2O), 1.2μL of each forward and reverse primers (10μM), 0.15μL of Taq DNA Polymerase (5 U/μL, MP Biomedicals) and 9.8μL of sterile H2O. ..

Article Title: Yeast diversity in Brazilian artisanal cheeses: Unveiling technologically relevant species to improve traditional cheese production
Article Snippet: Although less studied than bacterial biota, the presence of yeast during the artisanal cheese’s production is of fundamental importance.. Yeasts can prevent the growth of undesirable species in the cheese’s core and surface and actively participate in the aromatic development of these products.. On the other hand, reintroducing the most abundant yeast species can help mitigate potential health risks related to artisanal production.

Article Title: Repeated loss of function at HD mating-type genes and of recombination in anther-smut fungi
Article Snippet: .. Boiling PCR was performed in a final volume of 30 μL containing 3 μL of fungal suspension, 15 μL of 2X Buffer (containing a mix of dNTPs 5 mM in addition of bovine serum albumin (BSA) 20 mg/ml, W1 detergent 1%, home made 10X Buffer (Ammonium Sulfat 1 M, Tris HCl 1 M, MgCl 2 6H 2 O 1 M and sterile deionized H 2 O), 1.2 μL of each forward and reverse primers (10 μM), 0.15 μL of Taq DNA Polymerase (5 U/μL, MP Biomedicals) and 9.8 μL of sterile H 2 O. .. Boiling PCR was performed in a final volume of 30 μL containing 3 μL of fungal suspension, 15 μL of 2X Buffer (containing a mix of dNTPs 5 mM in addition of bovine serum albumin (BSA) 20 mg/ml, W1 detergent 1%, home made 10X Buffer (Ammonium Sulfat 1 M, Tris HCl 1 M, MgCl 2 6H 2 O 1 M and sterile deionized H 2 O), 1.2 μL of each forward and reverse primers (10 μM), 0.15 μL of Taq DNA Polymerase (5 U/μL, MP Biomedicals) and 9.8 μL of sterile H 2 O.

Sterility:

Article Title: Repeated loss of function at HD mating-type genes and of recombination in anther-smut fungi.
Article Snippet: .. Boiling PCR was performed in a final volume of 30μL containing 3μL of fungal suspension, 15μL of 2X Buffer (containing a mix of dNTPs 5mM in addition of bovine serum albumin (BSA) 20mg/ml, W1 detergent 1%, home made 10X Buffer (Ammonium Sulfat 1M, Tris HCl 1M, MgCl26H2O 1M and sterile deionized H2O), 1.2μL of each forward and reverse primers (10μM), 0.15μL of Taq DNA Polymerase (5 U/μL, MP Biomedicals) and 9.8μL of sterile H2O. ..

Article Title: Genetic diversity and evolution of endogenous pararetroviruses across Solanaceae : How farming systems drive dynamic tomato EPRVS changes under salt stress
Article Snippet: .. PCR reaction was performed in a final volume of 25 μl containing 100 ng of each primer, 5 μl of 10X buffer, 1 μl of dNTP (10 mM each), 1.5 μl MgCl 2 (1.5 mM), 1U of Taq DNA polymerase (MP Biomedicals, Europe), 2 μl of extracted DNA, and sterile water. .. PCR amplifications were performed on a T professional TRIO system thermocycler (Biometra, Germany) using the following cycling program: One first denaturation step of 3 min at 94°C followed by 35 amplification cycles, each consisting of 30 sec at 94°C, 50 sec at 55°C, 1 min at 72°C.

Article Title: Repeated loss of function at HD mating-type genes and of recombination in anther-smut fungi
Article Snippet: .. Boiling PCR was performed in a final volume of 30 μL containing 3 μL of fungal suspension, 15 μL of 2X Buffer (containing a mix of dNTPs 5 mM in addition of bovine serum albumin (BSA) 20 mg/ml, W1 detergent 1%, home made 10X Buffer (Ammonium Sulfat 1 M, Tris HCl 1 M, MgCl 2 6H 2 O 1 M and sterile deionized H 2 O), 1.2 μL of each forward and reverse primers (10 μM), 0.15 μL of Taq DNA Polymerase (5 U/μL, MP Biomedicals) and 9.8 μL of sterile H 2 O. .. Boiling PCR was performed in a final volume of 30 μL containing 3 μL of fungal suspension, 15 μL of 2X Buffer (containing a mix of dNTPs 5 mM in addition of bovine serum albumin (BSA) 20 mg/ml, W1 detergent 1%, home made 10X Buffer (Ammonium Sulfat 1 M, Tris HCl 1 M, MgCl 2 6H 2 O 1 M and sterile deionized H 2 O), 1.2 μL of each forward and reverse primers (10 μM), 0.15 μL of Taq DNA Polymerase (5 U/μL, MP Biomedicals) and 9.8 μL of sterile H 2 O.

Isolation:

Article Title: Yeast diversity in Brazilian artisanal cheeses: Unveiling technologically relevant species to improve traditional cheese production
Article Snippet: Although less studied than bacterial biota, the presence of yeast during the artisanal cheese’s production is of fundamental importance.. Yeasts can prevent the growth of undesirable species in the cheese’s core and surface and actively participate in the aromatic development of these products.. On the other hand, reintroducing the most abundant yeast species can help mitigate potential health risks related to artisanal production.



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