Review

pcdna3 1 expression vector (TaKaRa)

TaKaRa is a verified supplier

TaKaRa manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

TaKaRa pcdna3 1 expression vector
Pcdna3 1 Expression Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 7409 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcdna3+expression+vector/T4+DNA+Ligase/pm41148321-70-7-15
Average 97 stars, based on 7409 article reviews

pcdna3 1 expression vector - by Bioz Stars, 2026-10

97/100 stars

Images

Recombinant:

Article Title: Menaquinone-7 production by engineered Saccharomyces cerevisiae through de novo Synthesis and fermentation optimization.
Article Snippet: Menaquinone-7 (MK-7) is a vital subform of vitamin K2.. MK-7 comprises 2-methyl-1,4-naphthoquinone rings and seven isoprene unit side chains (Mahdinia et al. 2017).. MK-7 shows significant therapeutic potential and is used in pharmaceuticals, foods, and supplements for managing osteoporosis, cardiovascular calcification, and Parkinson’s disease (Fusaro et al. 2020; Schwalfenberg et al. 2017).

Plasmid Preparation:

Incubation:

Article Title: A core transcriptional regulatory circuitry controls super-enhancer-driven activation of LGR5 in colorectal cancer.
Article Snippet: .. Intra-molecular ligationwasperformed ina diluted system containing 2μg digested DNA with 400U T4 DNA ligase (Takara) at 22 °C for 4 h. The resulting chromatin was incubated at 65 °C overnight to reverse the cross-links. .. The DNA was purified using the QIAquick PCR purification Kit (Qiagen) for subsequent PCR analysis.

other:

Article Title: Computational framework-guided engineering of microbial transglutaminase enables site-specific conjugation of Trastuzumab.
Article Snippet: Microbial transglutaminase (MTGase) specifically catalyzes the conjugation between primary amine-containing molecules and glutamine residues, making it a promising enzymatic approach for producing homogeneous antibody-drug conjugates (ADCs).. However, this strategy faces challenges associated with glycan-induced steric hindrance, insufficient enzyme activity, and poor thermal stability.. In this study, we engineered MTGase from Streptomyces mobaraensis (MTGase-Sm) using a computational framework-guided strategy to enhance its catalytic cc for site-specific conjugation at the Q295 site of trastuzumab.

Staining:

Article Title: α-Herpesviruses exploit host ssDNA gap prevention pathways for GC-rich genome replication and reactivation.
Article Snippet: .. with 5–15% methanol Sinopharm Chemical Reagent Co.,Ltd Cat# C119690025 Crystal Violet Ammonium Oxalate Solution,2.5% For Gram Stain Solarbio Cat# G1061 PrimeSTAR GXL DNA Polymerase Takara Cat# R050Q T4 DNA Ligase NEB Cat# M0202S Streptavidin Beads 6FF MedChemExpress Cat# SA021005 ProLong Gold Antifade Mountant with DAPI Thermo Fisher Scientific Cat# P36931 Lipofectamine 2000 Thermo Fisher Scientific Cat# 11668500 Lipofectamine RNAiMAX Thermo Fisher Scientific Cat# 13778100 polybrene MedChemExpress Cat# HY-112735 Benzonase Nuclease Sigma-Aldrich Cat# 9025–65-4 Nylon Membrane N+(0.45μm) Solarbio Cat#YA1760 (Continued on next page) 20 Cell Reports 45, 118044, October 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays In situ Proximity Ligation Assay reagent Sigma-Aldrich CCL-81 Deposited data Raw data Mendeley Data https://doi.org/10.17632/prxnnnn39f.1 Experimental models: Cell lines Vero ATCC CCL-81

Membrane:

Marker:

Article Title: Development of a genetically engineered multivalent subunit vaccine against "struck", "braxy", lamb dysentery, and enterotoxemia in sheep.
Article Snippet: BL21 (DE3) competent cells were obtained from Beijing TransGen Biotech Co., Ltd. (Beijing, China). .. Restriction enzymes BamHI and XhoI, T4 DNA ligase, a 2000 DNA marker, sodium dodecyl sulfate, isopropyl-β-D-thiogalactopyranoside (IPTG), and Taq PCR Master Mix were provided by Takara Biotechnology Co., Ltd. (Dalian, China). .. Agarose, a DNA extraction kit, a DNA rapid purification and recovery kit, a plasmid rapid isolation kit, and a pre-stained protein marker were obtained from Beijing TransGen Biotech Co., Ltd. Ni-NTA spin columns (5 mL) and sizeexclusion chromatography columns (Superdex 2000) were obtained from GE Healthcare Bio-Sciences (Pittsburgh, PA, USA).

Polymerase Chain Reaction:

Similar Products

Image Search Results

$( A – E ) Distribution of ATP5H ( A ), NDUFC1 ( B ), NDUFV2 ( C ), SLC25A4 ( D ), and UCP2 ( E ) mRNAs across ribosome fractions (free RNA/RNP, 40S/60S/80S, light polysome, heavy polysome) from sgNC and sgMETTL1 BxPC-3 and MiaPaCa-2 cells, quantified by qRT-PCR. Bar graphs show the proportion of untranslated and translated fractions. ** P < 0.01 and *** P < 0.001, by 2-tailed, unpaired t test. ( F ) Relative protein levels of NDUFV2 and UCP2 in PDAC control and METTL1-KO cells by Western blotting. ( G ) Relative protein levels of NDUFV2 and UCP2 in PDAC control and valine tRNA–KD cells by Western blotting. ( H and I ) Oxygen consumption rate in control and METTL1-KO groups from BxPC-3 ( H ) and MiaPaCa-2 ( I ) cells by Seahorse Mito Stress testing. *** P < 0.001, by 1-way ANOVA followed by Dunnett’s multiple-comparison test. ( J and K ) Oxygen consumption rate in control and valine tRNA–KD group from BxPC-3 ( J ) and MiaPaCa-2 ( K ) cells by Seahorse Mito Stress testing. *** P < 0.001, by1-way ANOVA followed by Dunnett’s multiple-comparison test. Data are representative of 3 independent experiments. All data are presented as the mean ± SD.$

Journal: The Journal of Clinical Investigation

Article Title: METTL1-mediated m 7 G modification of valine tRNAs drives metabolic adaptation in pancreatic ductal adenocarcinoma cells

doi: 10.1172/JCI204312

Figure Lengend Snippet: ( A – E ) Distribution of ATP5H ( A ), NDUFC1 ( B ), NDUFV2 ( C ), SLC25A4 ( D ), and UCP2 ( E ) mRNAs across ribosome fractions (free RNA/RNP, 40S/60S/80S, light polysome, heavy polysome) from sgNC and sgMETTL1 BxPC-3 and MiaPaCa-2 cells, quantified by qRT-PCR. Bar graphs show the proportion of untranslated and translated fractions. ** P < 0.01 and *** P < 0.001, by 2-tailed, unpaired t test. ( F ) Relative protein levels of NDUFV2 and UCP2 in PDAC control and METTL1-KO cells by Western blotting. ( G ) Relative protein levels of NDUFV2 and UCP2 in PDAC control and valine tRNA–KD cells by Western blotting. ( H and I ) Oxygen consumption rate in control and METTL1-KO groups from BxPC-3 ( H ) and MiaPaCa-2 ( I ) cells by Seahorse Mito Stress testing. *** P < 0.001, by 1-way ANOVA followed by Dunnett’s multiple-comparison test. ( J and K ) Oxygen consumption rate in control and valine tRNA–KD group from BxPC-3 ( J ) and MiaPaCa-2 ( K ) cells by Seahorse Mito Stress testing. *** P < 0.001, by1-way ANOVA followed by Dunnett’s multiple-comparison test. Data are representative of 3 independent experiments. All data are presented as the mean ± SD.

Article Snippet: The NDUFV2 expression vector (pcDNA3.1/NDUFV2/myc-His) was obtained from Addgene (127491).

Techniques: Quantitative RT-PCR, Control, Western Blot, Comparison